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Citations for Bio-Rad :
1101 - 1150 of 2194 citations for Rabbit Anti Sheep IgG H+L FITC since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: The isolated sEVs and m/lEVs from 1.5 × 106 cells following 72 h of cell culture were lysed with 4X sample buffer (Bio-Rad) with β-mercaptoethanol (for detecting TSG101 and flotillin-1 ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were incubated for one h at room temperature (RT) with horseradish peroxidase (HRP)-coupled secondary Abs at 1:2000 for one h (all secondary Abs from Biorad, raised in goatanti-rat HRP Cat # 5204-2504 and anti-mouse HRP Cat # 1721011) ...
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bioRxiv - Synthetic Biology 2023Quote: ... and incubated for 24 h in a thermal gradient using a DNA Engine Tetrad 2 Peltier Thermal Cycler (Bio-Rad) with the lid set to 50°C ...
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bioRxiv - Neuroscience 2023Quote: ... membranes were subsequently blocked for 2 h at room temperature with 5% non-fat milk (Bio-Rad, Hercules, CA, USA) or 5% bovine serum albumin (BSA ...
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bioRxiv - Immunology 2023Quote: Experiments were carried out on hydrogel-coated glass slides (Nexterion Slide H, Schott) by using a BioOdyssey Calligrapher MiniArrayer (BioRad). A 14-point dilution series of RBD was prepared with a combination of a ½ and ⅓ diluting series ...
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bioRxiv - Cell Biology 2023Quote: ... the membrane was cut lengthwise into strips to isolate bands of particular molecular weights before being washed with TBST and incubated at room temperature for 1 H in a 5% blocking buffer solution (Biorad). The western blot strips were then incubated overnight at 4 C in a solution consisting of the primary antibody diluted into blocking buffer ...
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bioRxiv - Biochemistry 2022Quote: ... The PVDF membranes were then incubated with HRP-coupled secondary antibodies (1:10,000, CST) for 2 h at 25°C and visualized by ChemiDoc XRS (Bio-Rad) instrument.
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bioRxiv - Genomics 2022Quote: ... After incubation with HRP-coupled secondary antibody diluted in saturation buffer (1 h, room temperature), membranes were revelated using ECL kit (Pierce, 34076) on Chemidoc XRS+ imager (Biorad). Images were processed and analyzed using ImageJ software ...
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bioRxiv - Cancer Biology 2023Quote: ... the membranes were washed in 0.1% TBS-Tween and incubated with the secondary antibody (1:10000) for 1 h at room temperature (RT) and revealed by chemiluminescence (Clarity ECL western blotting substrates, BioRad) using ImageQuant LAS 500 chemiluminescence CCD camera (GE Healthcare Bioscience AB) ...
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bioRxiv - Molecular Biology 2024Quote: ... Gels were run at 150 V for 1 h and transferred onto 0.2 µm PVDF membranes using the High MW setting on the Trans-Blot Turbo (Bio-Rad). Membranes were blocked with 10% skim milk in TBS-T for 2h with gentle agitation at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... resuspended in medium and counted at different incubation times (0, 24, 48, 72 and 96 h) on a TC 20 automated cell counter (Biorad). The experiments were conducted in triplicate for each timepoint ...
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bioRxiv - Biophysics 2023Quote: ... and yeast cell growth was monitored by measuring the OD600 at 2 h increments in a microplate reader (Benchmark Plus, BioRad). The remainder of the 10 ml cultures was used to assess Pgp expression by Western blot analysis of microsomal membrane preparations using the monoclonal C219 anti-Pgp antibody.
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bioRxiv - Microbiology 2023Quote: ... The secondary antibodies (rabbit and mouse) were purchased from Biorad and the HRP signal was developed using Clarity Western ECL Substrate (Biorad).
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bioRxiv - Biochemistry 2023Quote: ... The secondary antibodies (rabbit and mouse) were purchased from Biorad and the HRP signal was developed using Clarity Western ECL Substrate (Biorad).
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bioRxiv - Microbiology 2022Quote: ... Samples for both HA and IgG assays were then passed through a Micro-Bio spin column (Bio-Rad) and eluted in 50 μL 1x sample buffer for 5 min at room temperature ...
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bioRxiv - Immunology 2019Quote: ... we measured bacterial growth by detection of luminescence at λ = 470 using a Spectramax L luminometer (Bio-Rad) or by dilution of infected cultures on BYCE agar plates for enumeration of CFU ...
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bioRxiv - Immunology 2023Quote: ... unreacted ITC-DTPA was removed by dialysis against 0.25 M ammonium acetate (NH4Ac) pH 5.4–5.5 (metal free) supplemented with 2 g/L Chelex (Bio-Rad) using 20,000 molecular weight cut-off dialysis cassettes (Slide-a-Lyzer ...
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bioRxiv - Biophysics 2022Quote: ... then 6 L of RO water with Ca2+ chelating beads (BIO-RAD Chelex 100 Resin Cat#142-1253), and finally 6 L of RO water alone ...
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bioRxiv - Microbiology 2023Quote: ... folic acid 500 μg/L) or on Columbia agar solid support enriched with 5% horse blood (COH) (Biorad). Anoxic conditions were generated in Gaspack (BD ...
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bioRxiv - Developmental Biology 2024Quote: ... 1 g SDS in 1 L distilled water) using the Mini-PROTEAN® Tetra electrophoresis system (Bio-Rad). Then ...
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bioRxiv - Immunology 2024Quote: ... SDS 10g/L) and transferred onto a PVDF membrane with the Trans-Blot Turbo Transfer System (Bio-Rad). The membrane was blocked for 2h with a blocking solution (TBS ...
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bioRxiv - Cancer Biology 2021Quote: ... 0.2–2.2 Mb, S. cerevisiae Ladder: Bio-Rad, 1703605; CHEF DNA Size Marker, 1–3.1 Mb, H. wingei Ladder: Bio-Rad, 1703667) and PFGE was performed using the CHEF Mapper XA System (Bio-Rad ...
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bioRxiv - Cell Biology 2019Quote: ... IL-10 and MCP-1 in cell culture supernatants at 24 and 96 h post-infection on the Bioplex 200 (Bio-Rad). The Bioplex Manager 6.1 software was used for data analysis (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: ... to pH 8.88 first for 5 min at 95 °C followed by 60 °C for 3 h on a T100 Thermal Cycler (Bio-Rad) with the heated lid set to 105 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Signals were developed by incubating with 1/5000 fold dilution of anti-rabbit or anti-mouse secondary antibodies for 1 h at room temperature and quantified using the Image Lab Software on a ChemiDoc XRS system (Bio-Rad).
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bioRxiv - Molecular Biology 2020Quote: Following electrophoretic separation, oligonucleotide-protein complexes were transferred by electro-blotting (330 mA, 1 h) onto specific positively charged nylon membranes (Bio-Rad) and detected using an anti-digoxigenin antibody conjugated with alkaline phosphatase.
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bioRxiv - Cell Biology 2022Quote: ... 100 μl of the cell suspension was spread on a single partitioned YPDA plate and incubated at 30°C for 48 h prior to imaging on a ChemiDoc imaging system (Bio-Rad). The area of all colonies ...
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bioRxiv - Microbiology 2019Quote: DNA fragments that co-precipitated with H-NSHA were quantified by real-time quantitative PCR using SsoFast EvaGreen Supermix (Bio-Rad) according to the manufacturer’s instructions and using gene-specific primers ...
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bioRxiv - Biochemistry 2021Quote: ... Western blot was performed using 8 or 10% mini gels followed by wet transfer (1 h, 100 V) to Western grade nitrocellulose (Bio-Rad). Blots were incubated in Intercept blocking buffer (LI-COR ...
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bioRxiv - Biochemistry 2021Quote: ... The cells were replenished with compounds every 24 h and counted on days 1-9 using automated cell counter (Bio-Rad). KYSE-520 cells were plated onto 96-well plates (500 cells per well ...
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bioRxiv - Plant Biology 2021Quote: ... benthamiana leaves was infiltrated with 1 mM luciferin 40 h after inoculation of the Agrobacteria and then imaged under the Gel Doc XR+ System (Bio-Rad) with the Bolt mode ...
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bioRxiv - Microbiology 2020Quote: ... Clarified lysates were incubated with 2 ml of glutathione resin and rotated for 1 h at 4°C in a 25 ml disposable drip column (Bio-Rad). Settled resin was washed with 25 column volumes (CV ...
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bioRxiv - Microbiology 2021Quote: ... Then the samples were treated at 60oC for 1 h and then enzyme deactivated 95oC for 15 min in a thermocycler (Bio-rad). After the treatment ...
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bioRxiv - Cell Biology 2021Quote: Whole-cell extracts obtained from 2·105 cells for HEK293 and 5·105 cells for Hela and A549 at 15 h post-transfection (p.t.) were separated by SDS-PAGE in 12% TGX Stain-Free gel (Bio-Rad, USA). After electrophoresis ...
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bioRxiv - Cell Biology 2022Quote: ... Western blotting was performed according to the manufacturer’s instructions with minor modifications (SDS-PAGE run at 110 V, transfer onto PVDF membrane for 1 h at 110 V (BIO-RAD)) ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were separated on a pre-heated 45 cm sequencing gel (45 °C, 65 W, 1 h) and visualized using a ChemiDoc Imaging System (Bio-Rad).
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bioRxiv - Plant Biology 2022Quote: ... RNA electrophoresis was performed at 200 V for 1 h and then RNA was transferred to a nylon membrane using MiniProtean 3 system (BioRad, USA). Transfer conditions were 100 V for 1 h at 4 °C in TBE buffer 1 × ...
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bioRxiv - Neuroscience 2022Quote: ... was performed for 2 h at room temperature and protein bands were visualized using the Clarity Western ECL Substrate (BIO-RAD).
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bioRxiv - Molecular Biology 2019Quote: ... the membrane was incubated overnight at 4°C with primary antibodies diluted 1:1000 followed by a further 1 h incubation in the corresponding horseradish peroxidase conjugated secondary antibody (Bio-Rad) at a 1:10000 dilution ...
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bioRxiv - Cancer Biology 2021Quote: ... Blots were incubated with the appropriate secondary antibodies for 1 h at room temperature and visualized using the Chemi-Doc XRS+ system (Bio-Rad). Primary antibodies used were anti-BCL-XL (abcam ...
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bioRxiv - Molecular Biology 2021Quote: ... The formaldehyde crosslinks were reversed by heating the samples for 1 h at 70 °C and RNA was extracted using PureZOL™ (BioRAD) as described in the RNA extraction section ...
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bioRxiv - Immunology 2019Quote: ... to nitrocellulose membranes using a constant voltage of 100 V for 2 h using the BioRad Midi PROTEAN Western blotting module according to the manufacturer’s instructions (BioRad, CA, USA). The membranes were blocked with blocking solution [Tris-buffered saline (TBS ...
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bioRxiv - Biochemistry 2020Quote: ... After 24 h incubation at 4 °C the chelating agent was removed using a desalting column (Econo-Pac 10DG from Bio-Rad), pre-equilibrated with 20 mM Hepes buffer ...
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bioRxiv - Biochemistry 2021Quote: ... was incubated at 55°C for 2 h and then purified by 6% non-denaturing PAGE using a Prep Cell apparatus (Bio-Rad).
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was incubated for 1 h at 4°C with 0.5 mL Profinity IMAC resin (Bio-Rad Laboratories, Hercules, CA) in a buffer containing 20 mM MOPS-Na (pH 7.4) ...
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bioRxiv - Microbiology 2021Quote: ... incubated with specific primary antibodies for 1 h and detected with horseradish peroxidase (HRP)-conjugated secondary antibodies by using ClarityTM Western ECL Blotting Substrate (Bio-Rad). For pulldown assays between cell extract and purified Nyx and MAG mutants ...
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bioRxiv - Biochemistry 2020Quote: ... in TBST/5% wt/vol non-fat milk (1 h, RT), washed (5x, TBST) followed by addition of ECL substrate (Bio-Rad) and imaged (ChemiDoc ...
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bioRxiv - Immunology 2021Quote: ... The primary antibody was removed the next day and the membrane was incubated with secondary antibody at room temperature for 1 h. Enhanced chemiluminescence substrate (Clarity Western ECL Substrate, Catalog no. 1705060) from Bio-Rad Laboratories (Mississauga ...
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bioRxiv - Molecular Biology 2020Quote: ... or in transfer buffer (25 mM Tris, 200 mM glycine, 20% ethanol) for 2 h at 52V with a liquid transfer system (Bio-Rad). Total proteins were revealed by Pierce® Reversible Stain (Thermo Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... for 1 h at room temperature and subsequently probed with appropriate secondary antibodies coupled to Horseradish peroxidase for 1 h at room temperature followed by chemiluminescent detection with ECL reagent (Bio-Rad).