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Citations for Bio-Rad :
9851 - 9900 of 10000+ citations for Hepatitis E ORF2 protein mouse Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Proteins were transferred to a 0.45 µm nitrocellulose membrane for proteins larger than 35 kDa and to 0.1 µm nitrocellulose or 0.2 µm PVDF membranes for proteins smaller than 35 kDa for 30 min using Trans-Blot Turbo transfer system (Bio-Rad, 1704150) or for 2.5 h using wet transfer system (TG buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Between 20-30 µg of protein was electrophoresed in 4-15% miniPROTEAN TGX Stain-Free pre-cast gels (BioRad, 4568084/4568085/4568086) using electrophoresis buffer [24.76 mM Tris Base (American Bio AB02000-05000) ...
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bioRxiv - Cell Biology 2023Quote: Equal amounts of proteins were separated on 8% reducing SDS-PAGE and transferred to Nitrocellulose membranes (Ref# 162-0112, BIO-RAD, Germany). Primary antibodies anti-SERCA2a and anti-SERCA2b targeted the specific ends of SERCA2a and SERCA2b (see supplement) ...
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bioRxiv - Neuroscience 2023Quote: ... The proteins in the gel were transferred to a nitrocellulose membrane by Trans-Blot® Turbo™ Transfer System (Bio-Rad Laboratories) at 25 V ...
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bioRxiv - Microbiology 2023Quote: ... Gels were loaded with samples containing 10 μg total protein before electrophoresis on a 16.5% Tris Tricine gel (Bio-Rad, Hercules, CA). Gels were stained with the Pierce Silver Stain kit (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were prepared for gel electrophoresis by adding a volume containing 20 ug protein to 3 uL 1 M DTT and 7.5 μL 4X Laemmli sample buffer (BioRad Cat. No. 1610747) and topping up to 30 μL with deionized water ...
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bioRxiv - Developmental Biology 2024Quote: ... of total protein in Laemmli Buffer were subjected to electrophoresis on precast polyacrylamide gels and transferred to PVDF membranes (Bio-Rad Laboratories). List of primary antibodies is provided in Supplementary Table 2 ...
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bioRxiv - Cell Biology 2024Quote: ... Protein was resolved using SDS-PAGE and transferred to the nitrocellulose membrane using a transfer system (Trans-Blot Turbo; Bio-Rad Laboratories). The membrane was blocked using 5% skimmed milk (American Bioanalytical ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred onto Trans-Blot® Turbo™ Mini PVDF Transfer membranes with the Trans-Blot Turbo Transfer System (Bio-Rad). Membranes were blocked with 5% skimmed milk in PBS with added Tween20 0.05% (Merck ...
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bioRxiv - Microbiology 2024Quote: ... The blots were washed three times in PBS-T and the proteins were visualized using Clarity Western ECL blotting substrate (Bio-Rad, #1705061) or SuperSignal West Femto (ThermoScientific ...
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bioRxiv - Cell Biology 2024Quote: ... The stain-free gel was UV-activated for 1 minute and imaged for total protein (BioRad Chemi Doc XR+, Hercules, CA, USA) with ImageLab software (BioRad Image Lab v6) ...
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bioRxiv - Biochemistry 2024Quote: ... and boiled at 95° C for 5 minutes before separation on 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad). In-gel fluorescence was imaged first ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were washed as before and protein bands visualised using the enhanced chemiluminescent substrate (ECL) with the ChemiDoc™ XRS system (BioRad, USA). Details of primary and secondary antibodies used are in Table 2.
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bioRxiv - Neuroscience 2024Quote: ... The proteins were then transferred from the gel to a nitrocellulose membrane using the Trans-Blot Turbo™ Transfer System (Bio-Rad) at 25V ...
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bioRxiv - Neuroscience 2024Quote: ... 30 µg of protein was loaded into each well of a 4-15% Stain-Free polyacrylamide gel (Bio-Rad, Hercules, CA, USA) and resolved via SDS-PAGE ...
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bioRxiv - Cell Biology 2024Quote: ... boiled for 5 min at 95°C and resolved by SDS-PAGE in 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (#4561086, BioRad). After electrophoresis ...
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bioRxiv - Cancer Biology 2024Quote: ... A molecular weight marker was added onto each gel for reference (Precision Plus Protein Dual Color, #1610374, Bio-Rad, Hercules, CA, USA). Transfer on PVDF membranes was done using Pierce Western blot transfer buffer (#35040 ...
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bioRxiv - Cell Biology 2024Quote: ... Then an equal amount of proteins were separated on a polyacrylamide gel with pre-cast gradient (Mini-PROTEAN® Precast Gels, acrylamide 4% −20%, Bio-Rad) and transferred to nitrocellulose membrane (Trans-Blot® Turbo ™ Midi Nitrocellulose Transfer Packs ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Proteins from total and bound fractions were separated by 7.5 % SDS-PAGE and transferred to polyvinylidene difluoride membranes (Bio-Rad, Hercules, CA). To reduce the background signal ...
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bioRxiv - Plant Biology 2024Quote: ... The protein bands containing PSII supercomplexes were cut out and eluted by a Model 422 Electro-Eluter (Bio-Rad, Hercules, CA, USA). The relative amount of PSII supercomplexes were quantified by dot immunoblot analysis with D1 antibody (Agrisera ...
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bioRxiv - Cancer Biology 2024Quote: ... Thirty micrograms of each protein sample were separated by SDS-PAGE and transferred onto a nitrocellulose membrane using the Transblot Turbo Transfer System (Bio-Rad, 1704150EDU). Non-specific binding sites on the membranes were blocked with 5% BSA in TBS-Tween 0.1% for 60 min ...
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The HUSH epigenetic repressor complex silences PML nuclear bodies-associated HSV-1 quiescent genomesbioRxiv - Microbiology 2024Quote: ... Protein extracts were boiled for 10 minutes and then loaded on polyacrylamide gels (TGX Stain-Free FastCast gels (Bio-Rad, 1610181/1610183) or home-made gels ...
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bioRxiv - Microbiology 2024Quote: ... 20 μg of total protein was loaded onto each lane of SDS-PAGE on a 10% acrylamide gel (BIO-RAD cat. #4561034) and run 45 min at 60 V followed by 45 min at 110 V ...
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bioRxiv - Microbiology 2024Quote: ... 8 μL of lysate were loaded in each lane and resolved on a 7.5% Mini-PROTEAN® TGX™ precast protein gel (Biorad 4561025) for PBP1a and PBP1b samples ...
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bioRxiv - Immunology 2024Quote: ... Electrophoresis was conducted in MES buffer at 100-120 V for 40 min with protein standards (NEB, P7119S, or Bio-Rad, #1610373). Proteins were transferred to nitrocellulose membrane and membranes blocked in 5% milk powder/PBS overnight at 4 °C ...
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bioRxiv - Physiology 2024Quote: ... Proteins were separated by SDS-PAGE and transferred to PVDF membranes using a semi-dry transfer cell (Bio-Rad, Hercules, CA, USA). Membranes were blocked with 5% non-fat dry milk in TBS-Tween for 1 h at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... The indicated total protein amount was loaded on SDS-PAGE gels (4-20% Mini-protean® TGX™ precast gels, [Bio-Rad] ...
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bioRxiv - Cell Biology 2024Quote: ... After running the samples by an SDS polyacrylamide gradient gel (4–20% Criterion™ TGX™ Precast Midi Protein Gel, Bio-Rad) and transferring them to a PVDF membrane (350mA/1h) ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were denatured at 95°C for 10 mins and subjected to SDS-PAGE in a 4–12% Criterion™ XT Bis-Tris Protein Gel (Bio-Rad) using XT MES Running Buffer (Bio-Rad) ...
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bioRxiv - Plant Biology 2024Quote: ... the immunoprecipitated and input proteins were separated on SDS–PAGE gels and transferred onto PVDF membranes via a Trans-Blot Turbo Transfer System (Bio-Rad, USA). After blocking the membranes with a solution of 5% skim milk prepared in PBST with 0.1% Tween 20 ...
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bioRxiv - Immunology 2024Quote: ... A total of 80 µg of protein homogenate were separated by SDS-PAGE and transferred onto PVDF membranes (Bio-Rad, Hercules, CA). Membranes were blocked with fat-free milk powder (5% w/v ...
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bioRxiv - Immunology 2021Quote: ... The monolayer was fixed with 4% paraformaldehyde and permeabilized with Triton-X100 and stained with mouse anti-NP IgG1 (clone AA5H, Bio-Rad Antibodies) followed by goat anti-mouse HRP (DAKO ...
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bioRxiv - Molecular Biology 2019Quote: ... The blot was washed 4 times with TBS-T and subsequently incubated with a goat anti-mouse-HRP antibody (Bio-Rad #1706516) for 30 minutes ...
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bioRxiv - Developmental Biology 2019Quote: ... Subsequent analysis was conducted on a 12% SDS-PAGE gel and blots were probed with either mouse α-V5 (SV5-Pk1, 1:1000, Bio-Rad), mouse α-HA (HA.11 ...
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bioRxiv - Neuroscience 2021Quote: ... The blotted membranes were rinsed three times with 0.05% Tween 20 in Tris-HCl-buffered saline (TBST) and incubated with horseradish peroxidase-conjugated goat polyclonal antibody against mouse IgG (Bio-Rad Laboratories) or guinea pig IgG (Bioss Inc. ...
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bioRxiv - Cancer Biology 2021Quote: ... membranes were washed 3x with TBS-T and incubated with either Goat Anti-Mouse IgG (H+L)-HRP Conjugate (Bio-Rad, #1721011) or Goat Anti-Rabbit IgG (H+L)-HRP Conjugate (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... The membrane was washed three times for 10 minutes with 1x PBS and incubated with Goat Anti-Mouse IgG (H+L)-HRP Conjugate (BioRad, #172-1011). Chemiluminescence was induced by incubation with SuperSignal West Femto Maximum Sensitivity Substrate (ThermoFisher ...
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bioRxiv - Microbiology 2021Quote: Bio-Plex assays for cytokines and chemokines were carried out in Bio-Plex 200 system using Bio-Plex Pro Mouse Cytokine Grp I Panel 23-Plex assay kit (Bio-Rad, M60009RDPD) following manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... Secondary antibodies used for Western blotting were: Donkey anti-mouse- and anti-rabbit-HRP (#172-1011 and #170-6515, Bio-Rad, USA). Secondary antibodies used for immunofluorescence were ...
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bioRxiv - Microbiology 2022Quote: ... rabbit polyclonal anti-nsP1 (both validated in our laboratory) [11] and mouse monoclonal anti-GAPDH (Cat. # VMA00046, Bio-Rad, Hercules, CA, USA). Then ...
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bioRxiv - Bioengineering 2022Quote: ... The PVDF membrane was then washed 3 × 10 min with TBST and incubated with horseradish peroxidase (HRP)-conjugated goat anti-mouse (diluted 1:20,000; Bio-Rad, Hercules, CA) secondary antibody for one hour at room temperature followed by 6 × 10 min washes with TBST and detection using the Radiance Plus chemiluminescence substrate (Azure Biosystems ...
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bioRxiv - Cancer Biology 2022Quote: ... slides were washed twice with PBS and incubated with secondary antibodies (Desmin and MYOD1: anti-rabbit-HRP, BioRad 170-6515; Myogenin: anti-mouse HRP, BioRad 170-6516) 1:500 in PBS-1% BSA ...
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bioRxiv - Microbiology 2022Quote: ... cells were heat-fixated in 96-well plates and a sandwich assay with NP-specific monoclonal antibody H16-L10-4R5 (www.atcc.org) and a peroxidase-coupled goat anti-mouse IgG HRP polyclonal antibody (Bio-Rad Laboratories GmbH, Germany) was carried out ...
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bioRxiv - Microbiology 2021Quote: ... The purity of the eluted fractions was assessed by SDS-PAGE and Western blot using a mouse anti-histidine antibody (BioRad, AbD Serotec).
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bioRxiv - Cancer Biology 2022Quote: ... Blots were incubated in secondary antibody for one hour at room temperature (anti-rabbit; Amersham, #NA934) (anti-mouse; Amersham, #NA931) and visualized with Clarity Western ECL Substrate (Bio-Rad, #1705061) on a ChemiDoc Imaging System (Bio-Rad).
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bioRxiv - Developmental Biology 2021Quote: ... Five hundred ng of total RNA (from 21EM15 cells or mouse lenses) or 10 ng of total RNA (from explants) were reverse transcribed using the iScript cDNa Synthesis Kit (Bio-Rad, CA) per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies used for Western blotting were: Goat anti-mouse- and anti-rabbit-HRP (#172-1011 and #170-6515, Bio-Rad, USA).
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bioRxiv - Cancer Biology 2022Quote: ... membranes were incubated with complementary HRP conjugated secondary antibody (sheep anti-mouse IgG-HRP, 1:10000, GE Healthcare, NA931; goat anti-rabbit IgG-HRP, 1:5000, Bio-Rad, 1706515 ...
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bioRxiv - Immunology 2022Quote: ... To each well 50 µL of 2 µg/mL of biotin-conjugated mouse anti-bovine IFN-γ mAb (CC302, Bio-Rad Antibodies) diluted in PBS was added and plates incubated for 2 hours at RT ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were rinsed 3x for 15 minutes in 1X TBST and incubated in the proper secondary antibody (Goat anti-mouse BioRad #170-6516 or Goat anti-rabbit BioRad #170-6515) for 1 hour ...