Labshake search
Citations for Bio-Rad :
9551 - 9600 of 10000+ citations for Hepatitis E ORF2 protein mouse Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: ... Proteins were separated by SDS-PAGE on 16% acrylamide gels immobilized by transfer to a PVDF membrane (Cat. #1620177, Bio-Rad) using a Trans-Blot Turbo Transfer System (Trans-Blot ®TurboTM ...
-
bioRxiv - Microbiology 2024Quote: ... protein concentrations were measured using the BCA assay and 20 μg of proteins from whole cell lysates were resolved on pre-cast 12% tris-glycine PAGE (Bio-Rad) followed by transfer to a LF-PVDF membrane using a Bio-Rad semi dry transfer apparatus ...
-
bioRxiv - Neuroscience 2024Quote: ... The protein chemiluminescence signal was obtained and visualized with the ChemiDocTM Touch Gel Imaging System (Bio-Rad Laboratories, Hercules, CA, USA). After FasII or NCAM1 detection ...
-
bioRxiv - Molecular Biology 2024Quote: ... as well as either Coomassie Blue staining (for the TGX Precast Protein Gel) or the ChemiDoc Touch Imaging System (Bio-Rad) (for the TGX Stain-Free™ Protein Gel).
-
bioRxiv - Neuroscience 2024Quote: ... Samples were split and electrotransferred using 4-20% Mini-PROTEAN TGX Stain-Free Protein Gels and the PVDF membrane Trans-Blot Turbo Transfer System (Bio-Rad Laboratories ...
-
bioRxiv - Neuroscience 2024Quote: ... Cells were immediately lysed in ice cold Radioimmunoprecipitation Assay (RIPA) buffer and protein was quantified using a Bradford Assay (Bio-Rad), per manufacturer’s instructions.
-
bioRxiv - Biochemistry 2024Quote: ... Samples were centrifuged at 15700 g for 30 min at 4°C and protein concentration was determined by Bradford assay (Bio-Rad). 600 µM of GppNHp Rab8a1-176 was titrated into the cell containing 30 µM bMERB domain and for full-length Mical1 ...
-
bioRxiv - Physiology 2024Quote: ... Proteins in the samples were separated by polyacrylamide gel electrophoresis at 200 V for 40 min and the separated proteins were transferred onto a polyvinylidene difluoride (PVDF) membrane using a wet transfer cell (Bio-Rad) at 100 mAmps (4°C overnight) ...
-
bioRxiv - Plant Biology 2024Quote: ... The proteins were then transferred to polyvinylidene difluoride membranes using NuPAGE Transfer Buffer using a Trans-Blot Turbo Transfer System (Bio-Rad) as per the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... The proteins were then transferred from the gel onto a nitrocellulose membrane using a semi-dry method (BioRad Trans-Blot Turbo). The membrane was blocked with 5% non-fat dry milk in 1X TBST for 1 hour ...
-
bioRxiv - Immunology 2024Quote: The pellet can be saved as a source of LPS and the soluble protein antigens were treated overnight with Affi-Prep Polymyxin Resin (Bio-Rad) to remove trace amounts of LPS remaining.
-
bioRxiv - Cell Biology 2024Quote: ... using the BioRad Mixed Molecular Weight Protein Transfer setting (7 min, 1.3 A, 25 V) on the Trans-Blot Turbo Transfer System (Bio-Rad, 1704150) with Trans-Blot Turbo Transfer Buffer (Bio-Rad ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... Samples were separated by SDS-PAGE using 7.5% gels or Any kD Mini-PROTEAN TGX Precast Protein Gels (Biorad, cat. 4569033) and transferred to PVDF membranes using a Trans-Blot Turbo Transfer System (Biorad ...
-
bioRxiv - Molecular Biology 2024Quote: ... for at least 30 min at 4 °C cleared by centrifugation and protein concentration was determined by Bradford assay (Bio-Rad).
-
bioRxiv - Molecular Biology 2024Quote: ... the cells were irradiated with 400 mJ/cm2 UV light to fixate the RNA-protein complexes by covalent cross-linking in the cells (GS Gene Linker UV Chamber, Bio-Rad); except for the control samples which were not UV-treated ...
-
bioRxiv - Plant Biology 2024Quote: ... the samples were combined with SureBeads™ Protein G (10 μL in 50% incubation buffer slurry; Bio-Rad, Hercules, CA, USA). After 3 h of incubation at 4 °C with rotation ...
-
bioRxiv - Neuroscience 2024Quote: ... 7µg of total protein was incubated in loading buffer as described above and loaded into AnykD Mini-PROTEAN TGX Precast Protein Gels (15 wells, 15uL; Bio-Rad). Electrophoresis and protein transfer were performed as above ...
-
bioRxiv - Cell Biology 2024Quote: ... For western blotting the proteins were transferred to polyvinylidene difluoride (PVDF) membranes using the Trans-Blot Turbo RTA Mini PVDF Transfer Kit (Bio-Rad) and the Trans-Blot Turbo Transfer System (Bio-Rad) ...
-
bioRxiv - Cell Biology 2024Quote: ... Proteins were visualized using horseradish-peroxidase-conjugated secondary antibodies followed by chemiluminescence detection with ECL (Clarity Western ECL substrate, Bio-Rad).
-
bioRxiv - Microbiology 2020Quote: ... Lysates were analyzed by Luminex profiling (Luminex Corporation) using the Bio-Plex Pro Mouse Chemokine 31-plex panel (Bio-Rad; 12009159) at a 1:2 dilution following the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2019Quote: ... and horseradish peroxidase (HRP)-conjugated goat anti-mouse immunoglobulin G (IgG) antibody (catalog no. 1721011, batch no. 64109318) were all purchased from Bio-Rad Laboratories (Hercules ...
-
bioRxiv - Cell Biology 2020Quote: ... membranes were incubated with StarBright Blue 700 Goat Anti-Mouse IgG secondary antibody (1:2500, Bio-Rad, Cat# 12004159, RRID: AB_2884948), StarBright Blue 520 Goat Anti-Rabbit IgG secondary antibody (1:2500 ...
-
bioRxiv - Microbiology 2020Quote: ... Excess antibody was washed away with 3 x TBST rinses before the membrane was incubated with anti-mouse secondary antibody (Bio-rad) conjugated to horseradish peroxidase at a 1:15000 dilution in TBST at 25°C for 1 hour ...
-
bioRxiv - Cell Biology 2020Quote: ... for the rapamycin blot or with the fluorescent StarBright Blue 700 anti-mouse (1:5000) and StarBright Blue 700 anti-rabbit (1:5000) secondary antibodies (Bio-Rad) for the MRT68921 blots in TBS-T containing 5% powder milk ...
-
bioRxiv - Cell Biology 2020Quote: ... Membranes incubated with HRP (horseradish peroxidase)–conjugated antibodies (HRP rabbit, 111 035 144; HRP mouse, 115 035 146) were developed using Clarity western ECL substrate solutions (Bio-Rad) with a ChemiDoc XRS+ imaging system (Bio-Rad).
-
bioRxiv - Immunology 2021Quote: ... Slides were incubated overnight at 4 °C with the following primary antibodies (1:100, in blocking buffer): mouse anti-human MBP (BMK-13, antihuman MBP (BMK-13, MCA5751 Bio-RAD), rabbit anti-human PD-L1 (E1L3N ...
-
bioRxiv - Developmental Biology 2021Quote: ... horseradish peroxidase-conjugated goat anti-rabbit IgG (1706565, 1:500) and goat anti-mouse IgG (1706516, 1:500) from Bio-Rad. DAPI were purchased from Invitrogen ...
-
bioRxiv - Neuroscience 2021Quote: ... CD11b+ cells were used for flow cytometric analysis after cells were stained with Alexa647-conjugated rat anti-mouse CD204 antibody (clone 2F8; Bio-Rad Laboratories GmbH ...
-
bioRxiv - Molecular Biology 2020Quote: ... Membranes were washed thrice with TBS-T before incubation for one hour at room temperature with secondary antibodies (horse radish peroxidase goat anti-mouse or anti-rabbit; Bio-Rad), all at 1:5000 dilution ...
-
bioRxiv - Molecular Biology 2020Quote: ... A gate was established for each factor using negative control samples stained with mouse IgG1 negative control antibodies (BIO-RAD, MCA928) or polyclonal rabbit IgG (R&D Systems ...
-
bioRxiv - Biochemistry 2021Quote: ... The membranes were then incubated with secondary antibodies (1:10000, goat anti-rabbit or goat anti-mouse IgG HRP conjugate (Bio-Rad) for 1 hour and detection was performed using the Immobilon-luminol reagent assay (EMP Millipore).
-
bioRxiv - Microbiology 2020Quote: ... or anti-PfADF1 (1:2000, (Wong et al, 2011)) and HRP-coupled goat anti-mouse or -rabbit secondary antibody (1:5000, STAR120P/STAR121P, Bio-Rad). sBlots were washed in PBST and detected using ECL reagent (Amersham ...
-
bioRxiv - Microbiology 2021Quote: ... and revelation with a IRDye 800CW Goat anti-mouse IgG secondary antibody (dilution 1/10000, Li-Cor Biosciences) using a ChemiDoc− Touch Imaging System (Bio-Rad).
-
bioRxiv - Microbiology 2021Quote: ... qRT-PCR was carried out with the gene-specific mouse primers using SsoAdvanced SYBR Green supermix (Bio-Rad, Hercules, CA, USA) and CFX96 thermal cycler (Bio-Rad ...
-
bioRxiv - Neuroscience 2020Quote: ... the membranes were further incubated in secondary antibody solutions (TBST with 5% Non-Fat Dry Skinned Milk and Horseradish Peroxidase conjugated secondary antibodies Goat Anti-Rabbit/Mouse, 1:10000, Bio-Rad) for 2 hours at room temperature ...
-
bioRxiv - Plant Biology 2021Quote: ... The blot was washed 3 times with TBST and incubated for 30 min in 1% milk prepared in TBST with anti-mouse HRP conjugate (Bio-Rad). Chemiluminescent detection was performed using SuperSignal West Dura Extended Duration (Thermo ...
-
bioRxiv - Microbiology 2020Quote: ... Plates were washed thrice before the addition of horseradish peroxidase (HRP)-conjugated anti-mouse IgG (H+L) (Bio-rad, 170-6516) at 1:3,000 ...
-
bioRxiv - Microbiology 2020Quote: ... followed by secondary horseradish peroxidase-conjugated anti-mouse or anti-rabbit immunoglobulin antibodies and chemiluminescence Clarity or Clarity max substrate (Bio-Rad). A Bio-Rad ChemiDoc imager was used.
-
bioRxiv - Neuroscience 2022Quote: ... followed by incubation for 2 hours at room temperature with HRP-conjugated goat anti-rabbit or goat anti-mouse secondary antibodies (Bio-Rad), diluted 1:5000 in 5% nonfat milk in TBS-T ...
-
bioRxiv - Cell Biology 2022Quote: ... were used for Western blotting together with anti-mouse IgG antibody conjugated with horseradish peroxidase (goat-polyclonal, 5178-2504, Bio-Rad). Chemicals were purchased from Sigma-Aldrich unless specified otherwise.
-
bioRxiv - Microbiology 2022Quote: ... membranes were incubated at 37°C for 1 h with goat anti-mouse IgG StarBright Blue 520 or anti-rabbit IgG Starbright Blue 700 (Bio-Rad) secondary antibodies ...
-
bioRxiv - Microbiology 2019Quote: ... Lungs were homogenized in D-PBS containing protease inhibitors to perform the cytokines quantification using a commercial multiplex mouse cytokine bead assay (Bio-Rad, Bio-Plex Pro Mouse Cytokine 23-plex Assay ...
-
bioRxiv - Molecular Biology 2019Quote: ... were purchased from Thermo Fisher Scientific while secondary antibodies Goat anti-mouse HRP (172-1011) and Goat anti-rabbit HRP (170-8241) were from Bio-Rad. MG132 (C2211 ...
-
bioRxiv - Genetics 2019Quote: ... blocked in 5% non-fat dry milk, primary blotting using mouse anti-mNeonGreen (1:1000, Chromtek 32F6) or hFAB™ Rhodamine Anti-Actin (12004164 BioRad), and secondary blotting using 1:3000 anti-mouse HRP (NXA931 ...
-
bioRxiv - Cancer Biology 2020Quote: ... StarBright Blue 520 Goat anti-Rabbit IgG and StarBright Blue 520 Goat anti-Mouse IgG (12005867) were purchased from Bio-Rad. Antibody against RIT1 (#53720 ...
-
bioRxiv - Molecular Biology 2020Quote: ... 1:1000)The membranes were then incubated with secondary antibodies (1:10000, goat anti-rabbit or goat anti-mouse IgG HRP conjugate (Bio-Rad) for 1hr and the detection was performed using the immobilon-luminol reagent assay (EMP Millipore).
-
bioRxiv - Plant Biology 2021Quote: ... membranes were incubated with HRP-conjugated secondary antibodies (rat (IgM)/LM20 and LM19 mouse (IgG)/2F4 (in 5% milk PBS) and after washing chemiluminescence induced with Clarity Western ECL Substrate (Biorad, #1705061) and detected with ChemiDoc imaging system (Biorad).
-
bioRxiv - Microbiology 2021Quote: ... Cytokines in the supernatants were then measured by using Bio-Plex Pro Mouse Cytokine 23-plex Assay (Biorad Laboratories, Hercules, CA) following the manufacturer’s standard protocol.
-
bioRxiv - Pathology 2020Quote: ... The sections were rinsed in PBS three times and incubated with Alexa Fluor 488- or Alexa Fluor 594-labelled goat anti-mouse (Bio-Rad Laboratories ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Plaques of FCoV were stained with specific antibodies (primary antibody: mouse anti feline coronavirus monoclonal antibody at 1:500 dilution solution, Bio-Rad; secondary antibody ...