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Citations for Bio-Rad :
9801 - 9850 of 10000+ citations for Hepatitis E ORF2 protein mouse Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... SDS-PAGE was performed using 10 μg of NP-40 extract protein mixed with an equal volume of 2X Laemmli buffer (BioRad cat#: 1610737) supplemented with 5% 2-mercaptoethanol (without boiling) ...
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bioRxiv - Cell Biology 2024Quote: ... Transfer of the proteins from gel to the Immun-Blot® Low Fluorescence PVDF membrane with low fluorescent transfer buffer (BIORAD; #1704275) was performed in Trans-Blot Turbo Transfer System (BIORAD ...
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bioRxiv - Cancer Biology 2024Quote: ... Gel electrophoresis was performed on 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad Laboratories AG, Cat#4561094/4561096). Proteins were transferred to Trans-Blot Turbo 0.2 µm Nitrocellulose Transfer Membrane (Bio-Rad ...
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bioRxiv - Biochemistry 2024Quote: ... The presence of the complex was assessed by 4-15% gradient SDS-PAGE using Mini-PROTEAN® TGX™ Precast Protein gels (BIORAD). Concentration of the RNAP in these fractions was determined by absorbance at 280 nm using NanoDrop™ One/OneC Microvolume UV-Vis Spectrophotometer (Thermofisher ...
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bioRxiv - Biophysics 2024Quote: ... The pellet was then resuspended in binding buffer (50mM Tris, 120 mM NaCl, pH 7.4) Bradford protein assay (Bio-Rad, Hercules, CA) was used to determine the protein concentration and the membranes were diluted to 1000 μg/mL and stored in a −80 °C freezer for later use ...
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bioRxiv - Cell Biology 2024Quote: ... The protein concentrations of cell lysates were measured using BCA assay (Thermo 23225) and equal amounts of proteins were loaded onto 4 – 15 % polyacrylamide gradient gels (Bio-Rad 4561084) and fractionated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Neuroscience 2023Quote: ... The eluted protein was subjected to an additional purification step using fast protein liquid chromatography (FPLC) and a Superdex 200 Increase 10/300 GL column (Cytiva, 28990944) in Chelex 100-treated (Bio-Rad, 1422832) dialysis buffer (25 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2023Quote: ... A normalized volume equivalent to 20 μg total protein in the predigested sample was resolved on a 4-12% Criterion XT Bis-Tris gel (Bio-Rad 3450124) at 100V for 2 hours ...
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bioRxiv - Immunology 2023Quote: ... A total of 30 µg of protein per well was loaded onto a 4-15% precast SDS-PAGE gel (Bio-Rad, 4561083). SDS-PAGE running conditions are as follows ...
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bioRxiv - Microbiology 2023Quote: ... Protein samples were fractionated by SDS-polyacrylamide gel electrophoresis using a 4%–20% Mini-PRO TEAN TGX Gel (BioRad, Hercules CA, USA), and the resolved proteins were transferred by electroblotting onto polyvinylidene difluoride membranes (Immobilon-P PVDF Transfer Membranes) ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were then vortexed for 1 min at max speed and loaded into a 10% sodium dodecyl sulfate (SDS)-polyacrylamide gel and run at constant 100 V for 1 h 45 min in running buffer (25 mM Tris, 190 mM Glycine, 3.46 mM SDS) alongside a Precision Plus Protein Dual Color Standard (Bio-Rad, Cat #1610374) in ...
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bioRxiv - Neuroscience 2022Quote: ... extracted proteins were first immobilized and resolved based on molecular weight on 4–15% Mini-PROTEAN TGX Precast gels (Bio-Rad Laboratories) through sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) ...
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bioRxiv - Developmental Biology 2023Quote: 30 to 60 µg of protein obtained from AT2 were separated on 4-20% Criterion TGX Gels (Bio-Rad. Cat. No. 5678093), transferred to PVDF membranes (Trans-Blot Turbo System ...
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bioRxiv - Neuroscience 2023Quote: ... A 150 µl aliquot of this crude homogenate was removed and protein concentration was determined using the Bradford method (BioRad, Hercules, CA). Subcellular fractionation by density gradient ultracentrifugation using Optiprep was performed on remaining 600 µl sample for the 6- and 12-month-old mice as previously described 47.
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bioRxiv - Neuroscience 2023Quote: ... Once ready for testing we quantified the amount of mitochondria from each isolation using Bio-Rad Protein Assay Kit (Bio-Rad, #5000001), plated 3µg/50µl/well onto a 96 well plate with all animals having 6-8 technical replicates (101085-004 ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatants were collected and stored at -80 until α5 subunit and its binding partners were immunoprecipitated using SureBeads protein G magnetic beads according to the manufacturer’s protocol (Biorad, Hercules, CA, USA). Western blots (detailed in section 2.8 ...
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bioRxiv - Biochemistry 2023Quote: ... and 30 μg of the proteins were loaded on 4-12% Criterion TGX Stain-Free precast polyacrylamide gels (BIO-RAD, Cat# 5678084) and transferred to nitrocellulose membranes which were subsequently blocked by 5% BSA for 1 hour at RT ...
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bioRxiv - Neuroscience 2023Quote: ... Equal amounts of protein were loaded onto 10% or 12% pre-cast Tris-HCl Mini-PROTEAN gels (Bio-Rad, Hercules, CA, USA) and proteins separated by electrophoresis ...
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ALS iPSC-derived microglia and motor neurons respond to astrocyte-targeted IL-10 and CCL2 modulationbioRxiv - Neuroscience 2023Quote: ... Equal amounts of protein were loaded onto 10% or 12% pre-cast Tris-HCl Mini-PROTEAN gels (Bio-Rad, Hercules, CA, USA) and proteins separated by electrophoresis ...
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bioRxiv - Microbiology 2023Quote: Volumes of lysates corresponding to 20 μg of protein were then run on a Criterion XT 4- 12% Bis-Tris Precast Gel (Bio-Rad, 3450124) in MES running buffer (50 mM MES ...
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bioRxiv - Neuroscience 2023Quote: ... Soluble proteins were separated using SDS-PAGE and transferred to a 0.45 µm nitrocellulose membrane (Cat# 1620115, Bio-Rad, Hercules, CA, USA). Primary antibodies (Table 1 ...
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bioRxiv - Neuroscience 2023Quote: ... An equal amount of protein samples was loaded and separated on an SDS-PAGE 7.5% PROTEAN TGX Stain-Free gel (Bio-Rad, Cat.# 4568024), then transferred to 0.2 µm PVDF membranes (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2023Quote: Recombinant VP1 protein and lysate prepared from Vero E6 cells infected with EMCV strain SING-M105 were separated by SDS-PAGE (Bio-Rad, USA) and the proteins transferred onto polyvinylidene fluoride membrane (Immobilion-P ...
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bioRxiv - Cell Biology 2023Quote: ... Equal protein loading was confirmed using a stain-free visualization system (Criterion TGX Stain-Free Precast Gels, BioRad Laboratories, Hatfield, PA, USA) and the protein transferred to 0.45 mm polyvinylidene difluoride (PVDF ...
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bioRxiv - Biochemistry 2023Quote: ... The determination of casein concentration by the Lowry method was performed using the DC™ protein assay kit purchased from Bio-Rad Laboratories (California ...
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bioRxiv - Biochemistry 2023Quote: ... GFP-Cdc42p and ubiquitin were detected under non-saturated conditions and normalized to the housekeeping protein Pgk1p using the Image Lab Software (Bio-Rad, Inc.). Wild-type cells and control conditions were set to a value of one and adjusted for other samples accordingly.
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bioRxiv - Bioengineering 2022Quote: ... Fifteen micrograms total protein extracts were separated by 4–20 % stain-Free SDS-PAGE gels and transferred onto polyvinylidene difluoride (PVDF) membranes (Bio-Rad Laboratories). Membranes were blocked using Tris-buffered saline (TBS ...
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bioRxiv - Biochemistry 2022Quote: ... Gel demonstrating protein purity was generated using by loading 1 μg of each protein onto a Mini-PROTEAN TGX 4-20% gradient gel (BIO-RAD, 4561096) and then staining with Coomassie blue.
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bioRxiv - Cancer Biology 2023Quote: ... and 1 μg/μL of total cellular protein from each sample was used to run a PAGE and transferred onto a PVDF membrane (Bio-Rad, 1704157). The membranes were soaked in a blocking solution of 5% BSA (AMRESCO ...
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bioRxiv - Biophysics 2023Quote: ... an aliquot of 25µg of total protein per sample was loaded into 4–20% Mini-PROTEAN® TGX™ precast gels (Bio-Rad) for SDS-PAGE separation ...
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bioRxiv - Genetics 2023Quote: ... and equal amounts of protein sample were then loaded onto a 4-20% gradient SDS-PAGE gel (Bio-Rad, cat no. 4561095) and run at 120V before being transferred to a PVDF membrane (Bio-Rad ...
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bioRxiv - Evolutionary Biology 2023Quote: ... purified protein fractions were separated with 10%–20% SDS-PAGE and transferred onto polyvinylidene difluoride (PVDF) membrane (Bio-Rad, Hercules, CA, USA) using a semi-dry blotter (ATTO ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatants of the cell lysates were normalized for total protein concentration according to Bradford assay (#5000006, Bio-Rad, Hercules, CA, USA), mixed with SDS loading dye ...
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bioRxiv - Cell Biology 2023Quote: ... The protein concentration of the supernatant was determined by Bradford measurement (64) according to the manufacturer’s instructions (Bio-Rad, cat n° 5000006).
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bioRxiv - Neuroscience 2023Quote: ... Supernatants were collected and 10 µl of protein sample was loaded in each well of precast Mini-PROTEAN TGX 4-20% gradient Gel (BIO-RAD 4561096). Following SDS-PAGE ...
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bioRxiv - Cell Biology 2023Quote: Proteins were resolved by SDS-PAGE using 4-20 % or 4-15% TRIS-glycine gradient midi gels (Bio-Rad, Hercules, CA, USA), in electrode buffer (0.1% w/v SDS ...
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bioRxiv - Neuroscience 2023Quote: ... 50 ug of protein was diluted with Laemmli buffer and loaded without heat denaturation onto a Criterion 10-20% tris-HCl gel (Bio-Rad, 3450043) run in tris-glycine SDS buffer ...
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bioRxiv - Bioengineering 2023Quote: ... 15 ug protein were loaded and separated using 4–15% Mini-PROTEAN® TGX™ Precast SDS-PAGE Gels (Bio-rad, #4561086). Samples were then transferred to nitrocellulose membranes for immunoblotting ...
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bioRxiv - Bioengineering 2023Quote: ... All samples with equal amounts of protein were loaded on gels and separated by reducing SDS-PAGE and transferred to PVDF membranes (Bio-Rad, US). Membranes were blocked with 5% milk powder and incubated with rabbit anti-human Sox2 (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2023Quote: ... the supernatant in a new tube received 20% glycerol and protein concentration was estimated by Bradford’s Dye Reagent Concentrate (BioRad Laboratories, Hercules, CA). For gel electrophoresis ...
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bioRxiv - Plant Biology 2023Quote: ... and proteins were separated in SDS/urea–PAGE (15% polyacrylamide, 6 M urea) (Laemmli, 1970) using mini-protean apparatus (Bio-Rad USA). These separated proteins were transferred to a nitrocellulose membrane using trans-blot apparatus (Bio-Rad USA) ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 mg of whole cell extract or 40-80 mg of liver homogenate was loaded on Mini-Protean TGX protein gel (4-20% or 7.5%) (Bio-Rad, Hercules, CA) and transferred to Immobilon-P membranes by electrophoresis (MilliporeSigma ...
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bioRxiv - Cancer Biology 2023Quote: ... were loaded on 10 % or 4–20 % Mini-PROTEANR TGX™ Precast Protein Gels and separated at 100 V in Tris/Glycine/SDS buffer in Mini-Protean Tetra System (Bio-Rad). The proteins were transferred to a polyvinylidene difluoride membrane (PVDF ...
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bioRxiv - Microbiology 2023Quote: ... extracellular proteins were separated on SDS 15% (m/v) polyacrylamide gels and electroblotted to Immun-Blot polyvinylidene difluoride membranes (Bio-Rad, USA) using a Mini Trans-Blot electrophoretic transfer cell (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: The interaction between nucleic acids (ligands) and the proteins (analytes) were measured by the surface plasmon resonance (SPR) technique using a ProteOn XPR36TM (Bio-Rad Laboratories). NLC Sensor chips precoated with NeutrAvidin (Bio-Rad Laboratories ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of protein samples (15 μg/lane) were subjected to analysis by 10% SDS-PAGE and transferred to a PVDF membrane (Bio-Rad, 1620264) using either semi-dry or wet electroblotting (for proteins with a molecular weight of 100 kDa and above) ...
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bioRxiv - Neuroscience 2023Quote: ... We loaded 50 μg of reduced protein per gel lane and performed transfer with Trans-Blot® TurboTM Transfer System (Bio-Rad) at 25 V for 7 min ...
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bioRxiv - Genetics 2023Quote: ... and luciferase activities were normalized to total protein levels in the WCEs extracts determined using the Bradford assay kit (Bio-Rad Laboratories).
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bioRxiv - Immunology 2023Quote: ... a total of 10–20 µg protein was prepared and loaded onto 4 to 20% gradient Mini-PROTEAN TGX Gels (Bio-Rad, 4561093) and electrophoretically separated at 135 V ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration was determined using a colorimetric assay based on the Bradford dye-binding method (Bio-Rad Protein Assay Dye Reagent, Bio-Rad Laboratories ...