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Citations for Bio-Rad :
9501 - 9550 of 10000+ citations for Hepatitis E ORF2 protein mouse Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: Seven DIV slice cultures were transfected with enhanced yellow fluorescent protein (eYFP) by particle-mediated gene transfer using a Helios Gene Gun (Bio-Rad), as described (Alonso et al. ...
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bioRxiv - Neuroscience 2023Quote: ... 10 ug of protein from cortical samples and 45ug of muscle samples were diluted in 4x Laemmli sample buffer (Bio-Rad). P3 brain samples were already directly resuspended in 4x Laemmli sample buffer ...
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bioRxiv - Neuroscience 2023Quote: ... The secondary antibody was washed away with PBS-T and stained proteins were detected by chemiluminescence (Clarity western ECL, Bio-rad). The western blot signal was acquired using Amersham Image Quant8000.
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bioRxiv - Cell Biology 2023Quote: ... The denatured protein samples (20 µg) were then separated on 4-20 % SDS-polyacrylamide gels using a Tris-glycine running buffer (Bio-Rad) and electroblotted onto nitrocellulose membranes (0.2 µm pore size ...
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bioRxiv - Microbiology 2023Quote: ... USA) and the relative optical density of protein bands was quantified using Quantity One Software from Bio-Rad (Hercules, CA, USA).
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bioRxiv - Immunology 2023Quote: ... Samples normalized to equal amounts were denatured in Laemmli’s buffer at 95°C for 10 min and resolved by SDS-PAGE using Criterion TM TGX TM Midi protein gels (Bio-Rad). Resolved proteins were transferred onto 0.2μM PVDF membrane by the Criterion TM Blotter (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: ... 35 µg of lysate was loaded on 10% SDS-PAGE after quantifying the protein concentration using Bradford assay kit (Bio-Rad). After transferring bands to nitrocellulose membrane Ponceau S staining was performed for checking equal loading of protein on SDS-PAGE ...
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bioRxiv - Microbiology 2023Quote: Dilution series containing equal amounts of protein were prepared and separated on 12% Mini-PROTEAN TGX gels (BioRad, Hercules, CA, USA) by SDS-PAGE and then transferred onto 0.2 µM polyvinylidene difluoride (PVDF ...
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bioRxiv - Cancer Biology 2023Quote: ... and the proteins were transferred to nitrocellulose membranes using a Turbo-Blot semidry transfer apparatus from Bio-Rad (Hercules, CA, USA). Immunoblotting was performed by incubating the membranes with 5% milk or bovine serum albumin for 60 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins (10–30 μg) were run on a 10% SDS–polyacrylamide gels and then transferred to a nitrocellulose membrane (Biorad®). The membranes were blocked in 5% lowfat milk-PBS-tween solution and then incubated overnight at 4 °C with primary antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... 200 μg aliquots of mitochondria were flash-frozen in liquid nitrogen after measuring the protein concentration by a Bradford assay (Bio-Rad) and stored at −80°C.
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of protein was denatured at 95°C for 5 min in 1x Laemmli buffer containing 5% β-mercaptoethanol and separated in 4-15% mini-PROTEAN TGX Precast Protein Gels (Biorad) by electrophoresis and blotted onto Nitrocellulose membrane (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein extracts were subjected to electrophoresis under reducing conditions on 4–15% polyacrylamide gels (Bio-Rad Laboratories, Inc., Hercules, CA) and subsequently transferred onto a nitrocellulose membrane (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2023Quote: ... 40-80 μg protein was separated on SDS-PAGE gels and transferred to polyvinylidene difluoride (PVDF) membranes (#162–0177; Bio-Rad). Membranes were blocked in phosphate buffered saline (PBS)-Tween with 5% non-fat dry milk ...
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bioRxiv - Cell Biology 2023Quote: ... 30 μg of total protein per sample was subjected to reducing and denaturing SDS-PAGE prior to Western transfer onto nitrocellulose membrane (Bio-Rad). After blocking with 5% skim milk in PBS containing 0.1% (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... Equal amounts of protein lysates (20 µg) were separated in 10% SDS-PAGE gels and transferred to PVDF membranes (Bio-Rad). The membranes were incubated with specific primary antibodies (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... For each protein of interest 10 µg of sample was loaded in a 4-15% Tris-Glycine gel (Bio-Rad, 4568083). The gel was transfered onto PVDF membrane (GenScript ...
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bioRxiv - Biochemistry 2023Quote: ... Bio-Safe™ Coomassie stain and Precision Plus Protein™ dual color standard were obtained from Bio-Rad (Hercules, CA, USA). Gamborg’s B-5 Medium was acquired from Life Technologies (Carlsbad ...
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bioRxiv - Biophysics 2023Quote: ... samples were processed using a Bio-Rad NGC Quest 10 Plus fast protein liquid chromatography (FPLC) apparatus with a 5 mL EconoFit Nuvia IMAC affinity column (Bio-Rad). To purify protein from the soluble fraction ...
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bioRxiv - Cancer Biology 2023Quote: ... different amounts of protein lysate were first loaded onto 12.5% SDS-PAGE test gels and proteins separated using the BioRad SDS-PAGE system (Biorad, Hercules, CA). Gels were run at 150 V for 50 min. ...
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bioRxiv - Neuroscience 2023Quote: ... 10 µg of proteins in Laemmli buffer and protein standard were loaded on 4%-15% Criterion TGX Stain-Free protein gel in running buffer TGS 1XL(all from Bio-Rad) and transferred to a 0.2Lµm PVDF membrane with the Trans-Blot Turbo RTA Midi Transfer Kit (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: ... proteins (20 μg per lane) were separated by SDS–polyacrylamide gel electrophoresis and transferred to a polyvinylidene difluoride membrane (Bio-Rad). Membranes were blocked in 4% milk in TBST [0.01 M tris (pH7.4) ...
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bioRxiv - Cell Biology 2023Quote: All the protein samples were separated by sodium dodecyl sulfate polyacrylamide (SDS)-PAGE and were transferred to nitrocellulose membrane (Bio-Rad laboratories ...
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bioRxiv - Molecular Biology 2023Quote: ... About 15 μg of protein samples from lysates were boiled at 95°C for 5 min in Laemmli sample buffer (Bio-Rad) and resolved in 4-15% gradient SDS-PAGE gels (Mini-Protean TGX ...
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bioRxiv - Neuroscience 2023Quote: ... All lysates were normalized using bicinchoninic acid (BCA; Pierce BCA Protein Assay Kit) and combined with 4x Laemmli buffer (Bio-Rad). We loaded 50 μg of reduced protein per gel lane and performed transfer with Trans-Blot® TurboTM Transfer System (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were separated by sodium-dodecyl-sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using Mini TGX gels (10%, Bio-Rad, Hercules, USA) according to standard protocols and transferred to nitrocellulose membranes (0.2 μm ...
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bioRxiv - Synthetic Biology 2024Quote: ... The purity and size of the proteins were validated on an SDS–PAGE gel (Mini-PROTEAN TGX and Mini-TETRA cell, Bio-Rad). Purified proteins were stored at –20 °C.
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bioRxiv - Bioengineering 2024Quote: ... SDS-PAGE was performed using 4–15% Mini-PROTEAN® TGX™ Precast Protein Gel (#4561084, Bio-Rad Laboratories, Inc, USA). Before and after the laser exposure ...
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bioRxiv - Biochemistry 2024Quote: ... pH 7.6 with HCl) for one hour and then divided for blotting based on the prestained protein ladder (Bio-Rad, 1610373). Primary antibodies (see antibody table for vendor and dilutions ...
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bioRxiv - Plant Biology 2024Quote: ... 5 μl supernatant was loaded onto 10% SDS polyacrylamide gels and the proteins were transferred onto PVDF membranes using the Trans-Blot Turbo RTA Transfer Kit (1704275, Bio-rad). Membranes were blocked in PBS-T (tablets 524650-1EA ...
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Pushed to the edge: hundreds of myosin 10s pack into filopodia and could cause traffic jams on actinbioRxiv - Cell Biology 2024Quote: ... and heated at 70°C for 10 min before loading onto a 4-20% Mini-PROTEAN TGX Stain-Free protein gel (BioRad, 4568095). The gel was run at 180 V for 45 min and imaged on a ChemiDoc ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were boiled for 5 min and 20 to 30 μg of protein were separated by SDS–polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes (Bio-Rad). Membranes were blocked in 3% milk in Tris-buffered saline with 0.1% Tween 20 (TBST ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were subjected to SDS-PAGE for 4 minutes (or until all the sample had entered the gel) at 200V (two wells per sample; 4-20% Mini-PROTEAN TGX precast protein gel, BIO-RAD), then stained with Coomassie blue for ten minutes before being washed 4 X with ddH2O (10 minutes each ...
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bioRxiv - Molecular Biology 2024Quote: ... and 0.1 vol of DTT 1M was added and the mixture heated at 95°C for 5 min before loading onto Any kD Mini-PROTEAN TGX Stain-Free protein gel (4568124, Bio-Rad) and migration in 1x Tris-Glycine-SDS buffer at 200V ...
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bioRxiv - Biochemistry 2024Quote: ... Fractions containing protein were pooled and desalted using the Econo-Pac® 10-DG Desalting Columns (Bio-Rad, Cat#732-2010). To this end ...
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bioRxiv - Cell Biology 2024Quote: ... The refolded protein was separated by size-exclusion chromatography (HiLoad 16/600 Superdex 200 pg, Cytiva) via FPLC (NGC, Bio-Rad) to remove protein aggregates ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were eluted from beads using 400 mM Imidazole and their concentrations were determined using a Bradford Assay (BioRad, Hercules, CA) with BSA standard ...
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bioRxiv - Neuroscience 2024Quote: ... Each gel was transferred to a methanol-activated Immobilon-FL PVDF membrane (MilliporeSigma) which was then UV-activated and imaged for total protein content on a Chemidoc MP (Bio-Rad). Membranes were blocked at room temperature for 1 hr in a 1:1 mixture of PBS and fluorescent blocking buffer (Rockland Immunochemicals ...
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bioRxiv - Neuroscience 2024Quote: ... Approximately 15 μg protein was separated by SDS-PAGE on a precast 4-12% Criterion XT Bis-Tris gel (Bio-Rad) and transferred to a nitrocellulose membrane using TurboBlot (Bio-Rad) ...
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bioRxiv - Plant Biology 2024Quote: ... Twenty-five μg of protein per well were analyzed in an SDS-PAGE using a Mini-PROTEAN Tetra electrophoresis system (Bio-Rad) and transferred to a Trans-Blot transfer nitrocellulose membrane (Bio-Rad) ...
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bioRxiv - Plant Biology 2024Quote: ... Stain-free gels were activated and total protein on gel were detected and recorded using a ChemiDoc™ Imaging System (BioRad).
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bioRxiv - Cancer Biology 2024Quote: ... ten micrograms of proteins were separated by SDS-PAGE and transferred onto 0.2 μm PVDF membranes (Bio-Rad Laboratories, Inc., 1704156) using the Trans-Blot Turbo Transfer System (Bio-Rad Laboratories ...
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bioRxiv - Bioengineering 2024Quote: The outcome of the reaction was analysed using native PAGE (4-20% Mini- PROTEAN TGX Precast Protein Gels, Bio-Rad, 4561096). 1 µL of the retic lysate reaction was diluted with 2 µL 1×PBS and mixed with 2× sample buffer containing 62.5 mM Tris ...
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bioRxiv - Bioengineering 2024Quote: ... Gels were stained for proteins using Coomassie R-250 and subsequent gel images were captured on a Chemidoc MP Imaging System (Bio-Rad).
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bioRxiv - Cancer Biology 2024Quote: ... Secondary antibodies were applied for 1 hour at room temperature (1:5,000; CST, #7074) before detecting protein bands at the BioRad ChemiDoc MP Imaging System using chemiluminescence (BioRad, 170-5061).
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bioRxiv - Microbiology 2024Quote: ... Fractions with the protein of interest were concentrated and further subjected to anion exchange chromatography on a Resource Q (Bio-Rad) 6 ml-column to remove remaining contaminants with a NaCl salt gradient from 50 mM to 500 mM ...
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bioRxiv - Microbiology 2024Quote: ... Samples were denatured for 10 min at 95°C and then 10 µl were loaded onto a precast 4-15% protein gel (Bio-Rad). Electrophoresis was performed by applying a constant voltage of 200 V in SDS running buffer (Tris/Glycine/SDS ...
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bioRxiv - Immunology 2024Quote: ... Proteins lysates were denatured with DTT and boiled prior to separation by SDS-PAGE and transferred onto PVDF membranes (Bio-Rad). Primary antibody incubations (Supplementary Table 2 ...
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bioRxiv - Cell Biology 2024Quote: ... A total 20 μg protein per extract was boiled in 1X reducing sample buffer and used for SDS-PAGE (BioRad, 4561083). Validation of gene knockdowns was performed by Western blotting using primary antibodies against STARD7 (Proteintech ...
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bioRxiv - Molecular Biology 2024Quote: ... boiled for 10 min at 95°C and examined by western blot using 4-15% Mini-PROTEAN TGX precast protein gels (BioRad #4561083). After blocking step with 10% non-fat milk for 1h at room temperature ...