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Citations for Bio-Rad :
9551 - 9600 of 10000+ citations for Human Protein N terminal asparagine amidohydrolase NTAN1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... The protein samples were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (PAGE) and transferred to a nitrocellulose membrane (Bio-Rad, Canada). The expression levels of ABCB1 ...
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bioRxiv - Microbiology 2020Quote: ... Forty μg of total proteins were separated by electrophoresis on 10% polyacrylamide gels and transferred to PVDF membranes (Bio-Rad, CA, USA). The membranes were blocked with 5% BSA in Tris-buffered saline for 1 h at room temperature ...
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bioRxiv - Microbiology 2021Quote: Cell lysates were run under reducing conditions on an Any kD™ Mini-PROTEAN® TGX Stain-Free™ Protein Gel (BioRad) and imaged using a ChemiDoc imaging system (Biorad) ...
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bioRxiv - Microbiology 2021Quote: ... The separated protein was transferred to a nitrocellulose membrane in Towbin buffer 1× using the Mini Trans-Blot system (Biorad, United States) at 120mA for 120 minutes in ice cold conditions ...
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bioRxiv - Biochemistry 2020Quote: ... Purification of the desalted bacteriocin protein was performed using an Uno S-6 prepacked monolith cation exchange column (12×55 mm, Bio-Rad, USA) on a FPLC system (BioLogic DuoFlow System ...
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bioRxiv - Neuroscience 2019Quote: Protein extracts were loaded at 20μg per lane and resolved by standard electrophoresis in 4-20% Mini-PROTEAN TGX Precast polyacrylamide protein gels (Bio-Rad, Hercules, CA). Gels were transferred onto polyvinyl difluoride membranes (PVDF ...
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Checkpoint adaptation in repair-deficient cells drives aneuploidy and resistance to genotoxic agentsbioRxiv - Cell Biology 2019Quote: ... using 7.5 % polyacrylamide gels for Rad53 detection or a gradient of 4-15 % polyacrylamide for all other proteins in Mini-PROTEAN® electrophoresis chambers (Bio-Rad). Proteins were transferred to 0.45 µm pore size nitrocellulose membranes (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... the desalted protein fraction was purified using the Uno S6 prepacked monolith cation exchange column (12 × 53 mm, 6 mL, Bio-Rad, USA). The eluted fraction containing purified bacteriocin was concentrated to 4.6 mg/mL in an Amicon centrifugal filter concentrator with a 3 kDa cutoff membrane (Millipore ...
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bioRxiv - Bioengineering 2020Quote: Mouse tissues from mdx4cv mice treated with or without AAV9-NG or AAV9-iNG were lysed with cold RIPA buffer supplemented with protease inhibitors and extracted protein samples were separated by SDS-PAGE (BioRad, 4-15%) and transferred onto Nitrocelluloase membranes (0.45 μm) ...
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bioRxiv - Pathology 2019Quote: ... 2019) Thirty micrograms of total proteins were separated by SDS-PAGE gel (Mini-Protean Precast Acrylamide Gels, Bio-Rad, Hercules, CA, USA) and further transferred to the nitrocellulose membrane (Trans-Blot Turbo transfer pack ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were detected by specific primary antibody and secondary antibody then visualized using the ChemiDoc MP Imaging System (Bio-Rad, Hercules, CA).
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bioRxiv - Immunology 2021Quote: ... 10 μg of total cell lysates proteins were separated by electrophoresis on 10% SDS polyacrylamide gels (previously prepared following the Bio-Rad protocol) and transferred to a polyvinylidene difluoride (PVDF ...
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bioRxiv - Genetics 2021Quote: ... Equal amounts of proteins (40 μg) were loaded onto 10% Tris-glycine gel (CriterionTM XT precast gel) using XT Tricine Running Buffer (Bio-Rad, USA). After electrophoresis ...
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bioRxiv - Cell Biology 2020Quote: ... and 8 μg of protein was added from each sample to a 26-well Criterion TGX 4-20% precast gel (Bio-rad, 5671095). Proteins were transferred to an Immun-Blot low flouresence PVDF membrane (Bio-rad ...
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bioRxiv - Physiology 2021Quote: ... the membranes were rinsed three times in TBS-T for 5 min and the proteins were visualized by enhanced chemiluminescense (BioRad, Clarity 1705061) in a BioRad Universal III Hood with Image Lab software v.6.0.1 ...
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bioRxiv - Biochemistry 2021Quote: Intensities of bands corresponding to RBD proteins were measured using Gel Doc 2000 and Image Lab software (Bio-Rad, Hercules, CA, USA) in order to measure protein expression levels ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... and 20 µg of protein from each sample was separated by SDS-PAGE on 4–20% precast polyacrylamide gel (Bio-Rad, 4561096). Western blot analyses were performed according to standard protocols using the following antibodies ...
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bioRxiv - Plant Biology 2021Quote: ... 20 μl of eluted protein samples were separated on SDS gel (12%) and transferred to PVDF membrane (Immun-Blot® PVDF, Bio-Rad) through semi-wet blotting protocol ...
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bioRxiv - Cell Biology 2021Quote: ... 1∼2 μg of total protein for each lane) was loaded on a precast 4-20% gradient polyacrylamide gel (PAGE, Bio-Rad: 4561096) and electrophoresed at a constant voltage of 200 V for 35 min ...
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bioRxiv - Developmental Biology 2021Quote: ... Proteins were separated by SDS-PGE on a 4-15% Mini-PROTEAN® TGX Stain-Free™ gel (Bio-Rad #456-8084) and transferred with the Trans-Blot® Turbo™ RTA Transfer Kit (Bio-Rad #170-4275 ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were centrifuged at 20,000 x g for 10 min at 4°C and protein concentration in the supernatant was determined by Bradford assay (Bio-Rad, 5000202) and normalized across samples ...
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bioRxiv - Cell Biology 2021Quote: ... and the protein concentration was determined by interpolation on to absorbance curves generated by recombinant protein standards using iMark™ Microplate Absorbance Reader (Bio-Rad).
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bioRxiv - Evolutionary Biology 2020Quote: ... 1-5 μg of purified antigen or 20 μg protein extract were loaded in a precast 4-20% acrylamide gel (BioRad 456-1094). Gels were run for the first 20-30 min at 40V ...
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bioRxiv - Immunology 2020Quote: ... the supernatant was discarded and the Protein G beads were resuspended in sterile PBS and transferred into Spin columns (Micro Bio-Spin Chromatography Columns, Bio-Rad, UK). The beads were washed 4 x with sterile PBS before addition of 117 µl Glycine pH 2.7 per sample and incubated for 5 min at room temperature ...
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bioRxiv - Immunology 2020Quote: ... 1ug of purified recombinant S RBD-His6 was diluted in PBS and Laemmli buffer to a final volume of 20 μl and added to a 7.5% Mini-PROTEAN® TGX™ Precast Protein Gel (BIO-RAD). Resolved proteins were then transferred to a PVDF membrane ...
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bioRxiv - Microbiology 2020Quote: ... or lacking (non-reducing) 100 mM β-mercaptoethanol and resolved by SDS-PAGE in a 4-20% Mini-PROTEAN TGX Precast Protein Gel (BIO-RAD). Proteins were transferred to nitrocellulose membranes using a Trans-Blot SD Semi-Dry Transfer Cell (BIO-RAD ...
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bioRxiv - Molecular Biology 2021Quote: ... Equal volumes of leaf protein were separated by SDS-PAGE (Mini-PROTEAN® TGX stain-free™ precast gels; Bio-Rad system) and cys-OLE was detected by immunoblotting as described in Winichayakul et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... even protein amounts were loaded and separated on mini-PROTEAN TGX precast 4-15% Bis-Tris gels (#4568085, Bio-Rad, Copenhagen, Denmark). Using a Bio- Rad Trans-Blot semidry transfer system proteins were transferred to a nitrocellulose membrane (#1704159 ...
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bioRxiv - Microbiology 2021Quote: ... and then proteins in the gel were transferred to PVDF membrane using Trans-Blot Turbo Mini PVDF Transfer Packs (Bio-Rad, USA) and Trans-Blot Turbo System (Bio-Rad) ...
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bioRxiv - Cell Biology 2021Quote: ... GFP-Cdc42p and ubiquitin were detected under non-saturated conditions and normalized to the housekeeping protein Pgk1p using the Image Lab Software (Bio-Rad, Inc.). Wild-type cells and control conditions were set to 1 and adjusted for other samples accordingly.
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bioRxiv - Cancer Biology 2020Quote: ... 50μg of proteins were electrophoresed in 8% or 12% polyacrylamide gel and then transferred to PVDF membranes (Bio-Rad Laboratory, CA, USA) overnight ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were separated by polyacrylamide gel electrophoresis (PAGE) on a 10% mini-PROTEAN® TGX™ precast gel (Bio-Rad, CA, USA) at 140 V and 300 mA for 1 h ...
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bioRxiv - Cancer Biology 2022Quote: ... prestained protein ladder were resolved on 10% acrylamide gels by SDS-polyacrylamide gel electrophoresis and electrophoretically transferred to nitrocellulose membrane (BioRad Cat. # 1620112) at 100 volts for 90 min.
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bioRxiv - Cancer Biology 2022Quote: ... An equal amount of protein from control and each experiment was loaded onto 4-20% SDS-PAGE gels (Mini-PROTEAN TGX Gels, Bio-Rad, USA) and transferred to the nitrocellulose membranes (Amersham™ Protran® 0.45 μM ...
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bioRxiv - Bioengineering 2022Quote: ... Samples (10-15ug protein/lane) were run by electrophoresis on pre-cast SDS-Page Stain Free Gels (Bio-Rad, Hercules, CA, USA). Separated proteins were transferred to low fluorescence PVDF membranes using the TransBlot Turbo (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... Quantification of band intensity were performed under non-saturated conditions and normalized to the Pgk1p protein using the Image Lab Software (Bio-Rad, Inc.).
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bioRxiv - Cell Biology 2022Quote: ... Samples and PageRulerTM Prestained Protein Ladder (ThermoScientific) were loaded onto Mini-PROTEAN® precast gradient (4-20 %) polyacrylamide gels (Bio-Rad Laboratories) and proteins were separated in an electric field of 90-120 V for varying time spans in SDS Running Buffer (25 mM Tris-base ...
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bioRxiv - Cancer Biology 2022Quote: Whole cell extracts of NB4 and HT93 were prepared using urea lysis buffer and 30-60μg total protein was loaded on a denaturing 12%-polyacrylamide gel self-cast gel (Bio-Rad Laboratories, CA). Proteins were transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Cell Biology 2022Quote: Equal concentration of proteins from EV samples (10μg) were loaded onto precast SDS-polyacrylamide gels (BioRad, Mini Protean TGX Cat# 456-1086) and transferred to Immobilon-E PVDF Transfer membrane (Sigma ...
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bioRxiv - Immunology 2022Quote: ... aliquots of frozen mEVs were thawed at 37°C and Bio-Rad Protein Assay (Bradford 1976) was performed following manufacturer’s instructions (500-0006 Bio-Rad. München, Germany). Samples were diluted with filtered PBS 1:50 and bovine serum albumin (BioRad Laboratories ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentration of the supernatant was determined using the BCA protein assay (Pierce™) and 15 µg was loaded on the gel (3450124, Bio-rad), which was next turbo transferred on nitrocellulose membrane (1704159 ...
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bioRxiv - Immunology 2022Quote: ... Peak fractions pooled and the concentrations of conjugated mi3 particles were determined using a Bio-Rad Protein Assay (Bio-Rad, Hercules, CA). Conjugated nanoparticles were characterized by electron microscopy imaging and SEC as shown in Figure S1C-E ...
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bioRxiv - Molecular Biology 2022Quote: ... Either 50 μg or 25 μg (puromycin blots only) of total protein was separated by SDS-PAGE and transferred to a PVDF membrane (Bio-Rad, 1620177) for blotting ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies used in this study were anti-myelin basic protein (MBP, rat, monoclonal, clone 12, Bio-Rad, cat# MCA409S, 1:1000), anti-Neurofilament 200 (NF200 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the whole cell membrane pellets were resuspended in buffer and protein concentration was estimated by dye binding (Coomassie Brilliant Blue R-250; Bio-Rad, USA). The binding reaction was carried out in a final volume of 0.1 ml of Na+-containing buffer (20 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2023Quote: ... Equal amounts of protein extract (20–30 μg) were resolved by SDS-PAGE (4–15% gradient precast TGX polyacrylamide gel, Bio-Rad Laboratories), followed by standard western blot procedure ...
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bioRxiv - Molecular Biology 2023Quote: ... The high molecular weight protein GCN1 was transferred using the Trans-Blot Turbo Transfer System (Bio-Rad, Cat# 1704150 and Cat# 1704158). Subsequently ...
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bioRxiv - Plant Biology 2023Quote: Equal amounts (10 µg) of nuclear proteins from different samples were separated using SDS– PAGE and electrotransferred to a nitrocellulose membrane (BioRad, 162-0115) for 1.5 h at constant 400 mA at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins (30 μg/lane) were separated on an SDS-polyacrylamide gel and transferred to a polyvinylidene difluoride (PVDF) membrane (Bio-Rad Laboratories). The membrane was immunoblotted for CSF1R (#MAB3291 ...
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bioRxiv - Neuroscience 2022Quote: ... Protein samples (∼15 μg) were loaded and separated on 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE, Bio-Rad, Hercules, CA), and transferred to polyvinilidene difluoride membranes (PVDF ...