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Citations for Bio-Rad :
9401 - 9450 of 10000+ citations for Human Protein N terminal asparagine amidohydrolase NTAN1 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Proteins were heated in reducing Laemmli sample buffer at 95°C and resolved in SDS-PAGE protein gel and transferred to PVDF membrane (Bio-Rad). Membranes were blocked using 5% BSA (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... 5 µl HeLa lysate was preincubated with 1 µl of accessory protein for 10 min at room temperature in PCR plates (HSP9601, Bio-Rad). Reaction was started by adding 2 µl reaction mix ...
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bioRxiv - Cell Biology 2022Quote: ... Total protein was resolved on 10% SDS–polyacrylamide gel electrophoresis gels and transferred onto PVDF membranes (Bio-Rad, catalog no. 1704156) by Trans-Blot Turbo (Bio-rad) ...
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The MuSK-BMP pathway maintains myofiber size in slow muscle through regulation of Akt-mTOR signalingbioRxiv - Cell Biology 2022Quote: ... Membranes were visualized by chemiluminescence and under epi-blue to visualize total protein No-Stain labeling using a ChemiDoc MP imaging system (Bio-Rad). Densitometric analysis was performed using ImageLab software (Bio-Rad).
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bioRxiv - Genetics 2022Quote: ... and 25µg of protein was loaded in each well and resolved on 4-15% gradient Tris-Glycine gel (Bio-Rad - 4561086). Semi-dry transfer was done onto 0.2µm Nitrocellulose membrane as per Trans-BlotTurbo Kit (Bio-Rad - 1704270 ...
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bioRxiv - Genetics 2022Quote: ... or 25 µg (mouse tissues) of proteins were loaded per lane on Any kD or 4-20% Criterion TGX stain-free precast gels (Bio-Rad) and transferred to Immobilon-FL PVDF membrane (Millipore ...
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bioRxiv - Plant Biology 2022Quote: ... The purity of the recombinant protein was evaluated by SDS-PAGE and Coomassie staining (ChemiDoc Imaging System; BioRad, Hercules, CA, USA), and the concentration of the purified recombinant protein was determined with a Bio-Rad protein assay kit using bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2022Quote: ... the displayed S-trimer protein bands on SDS-PAGE gels were scanned and quantified by ChemiDoc MP imaging system (Bio-Rad) and ImageJ.
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bioRxiv - Microbiology 2022Quote: ... All radioactive protein samples were separated by size on a 4-to-20% Mini-Protean TGX precast SDS-PAGE gel (Bio-Rad). The gel was then dried and imaged using a Typhoon phosphorimager (Molecular Dynamics GE Healthcare Life Sciences) ...
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bioRxiv - Microbiology 2022Quote: ... His6 tagged proteins were detected with anti-His6 primary antibody (Pierce; 1:6,000) and anti-mouse secondary antibody (Biorad; 1:10,000). GroEL was detected with anti-GroEL primary antibody (Pierce ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were heated at 95 °C for 5 min and loaded on 12% Mini-PROTEAN TGX precast protein gel (#4561043, Bio-Rad). The gel was run using 1x Tris/Glycine running buffer at 100 V for 90 min ...
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bioRxiv - Synthetic Biology 2022Quote: ... Obtained soluble and insoluble fraction (pellet of cell debris) was analyzed by SDS-PAGE using 4–20% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad) and Precision Plus Protein Dual Color Standard (Bio-Rad).
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bioRxiv - Systems Biology 2022Quote: ... semidry electrotransfer of proteins to a PVDF membrane was carried out in a Trans-Blot Turbo Transfer System (Bio-Rad, USA). The membrane was incubated in blocking buffer (PBST ...
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bioRxiv - Neuroscience 2022Quote: ... 200 µg of protein extracts were loaded and separated on a 10% SDS-PAGE gels and transferred onto PVDF membranes (Bio-Rad). Membranes were blocked with a 3% BSA in TBS-T (Tris-Buffered Saline with Tween-20 containing in mM ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Twenty-five µg of protein from each sample was ran (100 V, 100 min) on polyacrylamide gels (Cat. No. 5671095, Bio-Rad) using tris-glycine SDS running buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Protein samples (25 µg) were separated by electrophoresis on 4-15% mini-PROTEAN TGX precast gels (Bio-Rad, cat# 456-1083) and transferred to PVDF membranes (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... Thermal stability of the protein samples was measured in a CFX96 Dx Real-Time qPCR instrument (Bio-Rad, Hercules, CA, USA); program ...
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bioRxiv - Bioengineering 2023Quote: ... Equal amounts of the proteins (50 µg) were electrophoresed (125 volts, 1 h) on a 4-20% Mini protean TGX stain-free protein gel (Bio-Rad) and transferred onto nitrocellulose membranes (125 volts ...
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bioRxiv - Cell Biology 2023Quote: ... 20-40 μg of total protein was resolved on 10% SDS-PAGE gels followed by immunoblotting on PVDF membranes (Bio-rad). Membranes were developed by chemiluminescence (SuperSignal West Pico ...
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bioRxiv - Biochemistry 2022Quote: NKX2-5 homeodomain purity was evaluated through SDS-PAGE using 15 well Mini-PROTEAN TGX Precast Protein Gels® (Bio-Rad). Samples were prepared with 4X loading buffer containing β-mercaptoethanol (BME ...
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bioRxiv - Cell Biology 2023Quote: ... for protein extracted from cultured cells or brain sections and micro bicinchoninic acid (microBCA) for protein extracted from EVs according to the manufacturer’s instructions (Bio-Rad Laboratories).
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bioRxiv - Cancer Biology 2023Quote: ... and the samples were boiled for 5 minutes before being loaded on the gel (4-15% Criterion TGX Precast Midi Protein Gel, Bio-Rad). Proteins were separated by SDS-PAGE and blotted into a polyvinylidene fluoride (PVDF ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein samples were subjected to 10–12% SDS-PAGE and transferred to a polyvinylidene fluoride membrane (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Cell Biology 2023Quote: ... Protein content was confirmed by mixing 2 μL of each elution step with 200 μL Bradford solution (Protein assay dye reagent (Bio-Rad) diluted 1:5 in H2O) ...
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bioRxiv - Molecular Biology 2023Quote: ... and Precision Plus Protein Unstained Standards (#1610363) was resolved by SDS-PAGE using precast 4-20% MiniProtean TGX Stain Free Gels (Bio-Rad). The blot in Fig ...
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bioRxiv - Microbiology 2022Quote: ... and proteins were analysed either by western blot or by silver staining after electrophoretic separation on polyacrylamide SDS-PAGE gel (Bio-Rad 4–20% Criterion™ TGX Stain-Free™ Protein Gel ...
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bioRxiv - Bioengineering 2023Quote: ... Approximately 5 x 108 vesicles and purified HaloTag standard curve were loaded and run on a 12% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad) at 150 V for 90 min or 50V for 10 min followed by 100V for approximately 70 min ...
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bioRxiv - Microbiology 2023Quote: ... equivalent total protein quantities from membrane samples were resolved by SDS-PAGE using Any kDTM polyacrylamide gels (Bio-Rad, Hercules, CA). Proteins transferred to a nitrocellulose membrane were probed with a monoclonal anti-His6 antibody (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... 25 µg or 50 µg of normalized protein for LapA-HA or MapA-HA tagged strains respectively was mixed with 4x Laemmli Sample Buffer (Bio-Rad) and 2-mercaptoethanol to a 1x working dilution and samples were boiled for 5 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... 10-30 μg of protein extracts were run at 100V for 1 hr on 10% pre-cast SDS-PAGE gels (Bio-Rad), then electro-transferred to 0.2 μm nitrocellulose membranes using the Trans-Blot Turbo Transfer system (Bio-Rad) ...
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bioRxiv - Physiology 2024Quote: ... Proteins in the samples were separated by polyacrylamide gel electrophoresis at 200 V for 40 min and the separated proteins were transferred onto a polyvinylidene difluoride (PVDF) membrane using a wet transfer cell (Bio-Rad) at 100 mAmps (4°C overnight) ...
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bioRxiv - Plant Biology 2024Quote: ... The proteins were then transferred to polyvinylidene difluoride membranes using NuPAGE Transfer Buffer using a Trans-Blot Turbo Transfer System (Bio-Rad) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... using the BioRad Mixed Molecular Weight Protein Transfer setting (7 min, 1.3 A, 25 V) on the Trans-Blot Turbo Transfer System (Bio-Rad, 1704150) with Trans-Blot Turbo Transfer Buffer (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... The proteins were then transferred from the gel onto a nitrocellulose membrane using a semi-dry method (BioRad Trans-Blot Turbo). The membrane was blocked with 5% non-fat dry milk in 1X TBST for 1 hour ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Samples were separated by SDS-PAGE using 7.5% gels or Any kD Mini-PROTEAN TGX Precast Protein Gels (Biorad, cat. 4569033) and transferred to PVDF membranes using a Trans-Blot Turbo Transfer System (Biorad ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were visualized using horseradish-peroxidase-conjugated secondary antibodies followed by chemiluminescence detection with ECL (Clarity Western ECL substrate, Bio-Rad).
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bioRxiv - Neuroscience 2024Quote: ... 7µg of total protein was incubated in loading buffer as described above and loaded into AnykD Mini-PROTEAN TGX Precast Protein Gels (15 wells, 15uL; Bio-Rad). Electrophoresis and protein transfer were performed as above ...
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bioRxiv - Immunology 2024Quote: The pellet can be saved as a source of LPS and the soluble protein antigens were treated overnight with Affi-Prep Polymyxin Resin (Bio-Rad) to remove trace amounts of LPS remaining.
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bioRxiv - Plant Biology 2024Quote: ... the samples were combined with SureBeads™ Protein G (10 μL in 50% incubation buffer slurry; Bio-Rad, Hercules, CA, USA). After 3 h of incubation at 4 °C with rotation ...
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bioRxiv - Synthetic Biology 2024Quote: ... The purity and size of the proteins were validated on an SDS–PAGE gel (Mini-PROTEAN TGX and Mini-TETRA cell, Bio-Rad). Purified proteins were stored at –20 °C.
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bioRxiv - Cell Biology 2024Quote: ... The refolded protein was separated by size-exclusion chromatography (HiLoad 16/600 Superdex 200 pg, Cytiva) via FPLC (NGC, Bio-Rad) to remove protein aggregates ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were subjected to SDS-PAGE for 4 minutes (or until all the sample had entered the gel) at 200V (two wells per sample; 4-20% Mini-PROTEAN TGX precast protein gel, BIO-RAD), then stained with Coomassie blue for ten minutes before being washed 4 X with ddH2O (10 minutes each ...
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bioRxiv - Cancer Biology 2024Quote: ... Secondary antibodies were applied for 1 hour at room temperature (1:5,000; CST, #7074) before detecting protein bands at the BioRad ChemiDoc MP Imaging System using chemiluminescence (BioRad, 170-5061).
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bioRxiv - Biochemistry 2024Quote: ... pH 7.6 with HCl) for one hour and then divided for blotting based on the prestained protein ladder (Bio-Rad, 1610373). Primary antibodies (see antibody table for vendor and dilutions ...
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Pushed to the edge: hundreds of myosin 10s pack into filopodia and could cause traffic jams on actinbioRxiv - Cell Biology 2024Quote: ... and heated at 70°C for 10 min before loading onto a 4-20% Mini-PROTEAN TGX Stain-Free protein gel (BioRad, 4568095). The gel was run at 180 V for 45 min and imaged on a ChemiDoc ...
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bioRxiv - Biochemistry 2024Quote: ... Fractions containing protein were pooled and desalted using the Econo-Pac® 10-DG Desalting Columns (Bio-Rad, Cat#732-2010). To this end ...
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bioRxiv - Bioengineering 2024Quote: ... Gels were stained for proteins using Coomassie R-250 and subsequent gel images were captured on a Chemidoc MP Imaging System (Bio-Rad).
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bioRxiv - Physiology 2024Quote: ... appropriate volumes of protein were combined with 4x Laemmli sample buffer supplemented with 10% β-mercaptoethanol (#1610710, Bio-Rad, Hercules, CA) and loaded onto a Bio-Rad 4%–20% gradient SDS-polyacrylamide gel ...
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bioRxiv - Molecular Biology 2024Quote: ... samples were denaturated for 5 minutes at 100°C upon addition of laemmli sample buffer and samples loaded on 4–15% Mini-Protean TGX precast protein gels (Bio-Rad).
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bioRxiv - Molecular Biology 2024Quote: ... samples were denaturated for 5 minutes at 100°C upon addition of laemmli sample buffer and samples loaded on 4–15% Mini-Protean TGX precast protein gels (Bio-Rad).