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Citations for Bio-Rad :
9451 - 9500 of 10000+ citations for Human Protein N terminal asparagine amidohydrolase NTAN1 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... 0.5% NP-40) and once with TE buffer and proteins were eluted in 50 μl of 4X Laemmli loading buffer (Bio-Rad) and boiled 10 minutes at 95C.
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bioRxiv - Neuroscience 2024Quote: ... Proteins were resolved in 1x Laemelli reducing buffer by SDS-PAGE electrophoresis and transferred to a Nitrocellulose blotting membrane (Bio-Rad). Subsequently ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins were then transferred to nitrocellulose membranes using Trans-Blot® Turbo (TM Transfer System, Biorad, catalog no.170-01918). Membranes were blocked using 1x TBST (0.2 mM Tris Base pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... ∼50 μg total protein from each sample was boiled for 10 min in 1X SDS PAGE protein loading buffer and then separated by electrophoresis through 4-20% gradient gels (Bio-Rad). The separated proteins were transferred to a nitrocellulose membrane (iBlot Transfer Stack ...
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bioRxiv - Microbiology 2024Quote: ... Samples were adjusted according to cell pellet protein content and ran on a pre-cast 10% TGX acrylamide gel (Bio-Rad) with the Precision Plus Protein Dual Color Standards ladder (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... and the same transferred membrane was stripped and re-probed for Staphylococcus Protein A detection with HRP-conjugated Anti-rabbit (Bio-Rad) at 1/3000 dilution.
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bioRxiv - Microbiology 2024Quote: ... The same volume of each sample was separated on a 12.5% SDS-PAGE gel and proteins were transferred to a blotting membrane (Immunobilon-P) using a wet mini trans-blot cell (Bio-Rad) at 4°C ...
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bioRxiv - Genomics 2024Quote: ... Proteins were extracted as described previously,53 and separated with sodium dodecyl sulfate-polyacrylamide gel electrophoresis using 12% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad). Transfer to the membrane was performed with iBind Western System (Thermo Fisher Scientific ...
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Transcription factors SlMYB41, SlMYB92 and SlWRKY71 regulate gene expression in the tomato exodermisbioRxiv - Plant Biology 2024Quote: ... we boiled the lysates for 1 min at 96°C and we loaded them on a 4% to 15% mini-PROTEAN TGX stain-free protein gels (Bio-Rad). We transferred the proteins onto a polyvinylidene difluoride (PVD ...
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bioRxiv - Immunology 2024Quote: ... Thirty micrograms of total protein were subjected to SDS-PAGE on a 10% gel in reducing conditions and transferred to PVDF membranes (Bio-Rad). To block nonspecific interactions ...
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bioRxiv - Molecular Biology 2024Quote: ... as well as either Coomassie Blue staining (for the TGX Precast Protein Gel) or the ChemiDoc Touch Imaging System (Bio-Rad) (for the TGX Stain-Free™ Protein Gel).
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bioRxiv - Neuroscience 2024Quote: ... Samples were split and electrotransferred using 4-20% Mini-PROTEAN TGX Stain-Free Protein Gels and the PVDF membrane Trans-Blot Turbo Transfer System (Bio-Rad Laboratories ...
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bioRxiv - Cancer Biology 2024Quote: ... ten micrograms of proteins were separated by SDS-PAGE and transferred onto 0.2 μm PVDF membranes (Bio-Rad Laboratories, Inc., 1704156) using the Trans-Blot Turbo Transfer System (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were eluted from beads using 400 mM Imidazole and their concentrations were determined using a Bradford Assay (BioRad, Hercules, CA) with BSA standard ...
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bioRxiv - Plant Biology 2024Quote: ... Stain-free gels were activated and total protein on gel were detected and recorded using a ChemiDoc™ Imaging System (BioRad).
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bioRxiv - Plant Biology 2024Quote: ... Twenty-five μg of protein per well were analyzed in an SDS-PAGE using a Mini-PROTEAN Tetra electrophoresis system (Bio-Rad) and transferred to a Trans-Blot transfer nitrocellulose membrane (Bio-Rad) ...
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bioRxiv - Neuroscience 2024Quote: ... Approximately 15 μg protein was separated by SDS-PAGE on a precast 4-12% Criterion XT Bis-Tris gel (Bio-Rad) and transferred to a nitrocellulose membrane using TurboBlot (Bio-Rad) ...
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bioRxiv - Neuroscience 2024Quote: ... Each gel was transferred to a methanol-activated Immobilon-FL PVDF membrane (MilliporeSigma) which was then UV-activated and imaged for total protein content on a Chemidoc MP (Bio-Rad). Membranes were blocked at room temperature for 1 hr in a 1:1 mixture of PBS and fluorescent blocking buffer (Rockland Immunochemicals ...
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bioRxiv - Bioengineering 2024Quote: The outcome of the reaction was analysed using native PAGE (4-20% Mini- PROTEAN TGX Precast Protein Gels, Bio-Rad, 4561096). 1 µL of the retic lysate reaction was diluted with 2 µL 1×PBS and mixed with 2× sample buffer containing 62.5 mM Tris ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were centrifuged at 15700 g for 30 min at 4°C and protein concentration was determined by Bradford assay (Bio-Rad). 600 µM of GppNHp Rab8a1-176 was titrated into the cell containing 30 µM bMERB domain and for full-length Mical1 ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were separated by SDS-PAGE on 16% acrylamide gels immobilized by transfer to a PVDF membrane (Cat. #1620177, Bio-Rad) using a Trans-Blot Turbo Transfer System (Trans-Blot ®TurboTM ...
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bioRxiv - Microbiology 2024Quote: ... Samples were denatured for 10 min at 95°C and then 10 µl were loaded onto a precast 4-15% protein gel (Bio-Rad). Electrophoresis was performed by applying a constant voltage of 200 V in SDS running buffer (Tris/Glycine/SDS ...
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bioRxiv - Microbiology 2024Quote: ... Fractions with the protein of interest were concentrated and further subjected to anion exchange chromatography on a Resource Q (Bio-Rad) 6 ml-column to remove remaining contaminants with a NaCl salt gradient from 50 mM to 500 mM ...
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bioRxiv - Cell Biology 2024Quote: ... A total 20 μg protein per extract was boiled in 1X reducing sample buffer and used for SDS-PAGE (BioRad, 4561083). Validation of gene knockdowns was performed by Western blotting using primary antibodies against STARD7 (Proteintech ...
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bioRxiv - Molecular Biology 2024Quote: ... for at least 30 min at 4 °C cleared by centrifugation and protein concentration was determined by Bradford assay (Bio-Rad).
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bioRxiv - Molecular Biology 2024Quote: ... the cells were irradiated with 400 mJ/cm2 UV light to fixate the RNA-protein complexes by covalent cross-linking in the cells (GS Gene Linker UV Chamber, Bio-Rad); except for the control samples which were not UV-treated ...
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bioRxiv - Molecular Biology 2024Quote: ... boiled for 10 min at 95°C and examined by western blot using 4-15% Mini-PROTEAN TGX precast protein gels (BioRad #4561083). After blocking step with 10% non-fat milk for 1h at room temperature ...
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bioRxiv - Immunology 2024Quote: ... Proteins lysates were denatured with DTT and boiled prior to separation by SDS-PAGE and transferred onto PVDF membranes (Bio-Rad). Primary antibody incubations (Supplementary Table 2 ...
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bioRxiv - Neuroscience 2024Quote: ... The protein chemiluminescence signal was obtained and visualized with the ChemiDocTM Touch Gel Imaging System (Bio-Rad Laboratories, Hercules, CA, USA). After FasII or NCAM1 detection ...
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bioRxiv - Microbiology 2024Quote: ... protein concentrations were measured using the BCA assay and 20 μg of proteins from whole cell lysates were resolved on pre-cast 12% tris-glycine PAGE (Bio-Rad) followed by transfer to a LF-PVDF membrane using a Bio-Rad semi dry transfer apparatus ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were immediately lysed in ice cold Radioimmunoprecipitation Assay (RIPA) buffer and protein was quantified using a Bradford Assay (Bio-Rad), per manufacturer’s instructions.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using 4-20% Mini-PROTEAN TGX precast protein gels (Bio-Rad) and were transferred onto a nitrocellulose membrane using wet western blot transfer ...
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bioRxiv - Physiology 2024Quote: ... Protein was transferred to a methanol-activated PVDF membrane (IPFL85R, sigma) at 30 V overnight at 4°C (1703930, Bio-Rad). The membrane was then washed with 1x TBST with 2% SDS ...
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bioRxiv - Bioengineering 2024Quote: ... Samples collected from undifferentiated iPSCs every odd passage from 35 to 49 were loaded onto 4–20% Criterion TGX Precast Protein Gels (Bio-Rad) at a total protein concentration of 50 µg ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were separated by electrophoresis at 120 Volts for 1.5 h and then electroeluted onto a PVDF membrane (BIO-RAD, #10026933) at 25 Volts ...
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bioRxiv - Neuroscience 2024Quote: ... The seeds were concentrated by immunoprecipitation after labelling by a C-20 antibody and precipitation with protein A-conjugated magnet beads (Bio-Rad), and then eluted by the SDS sample buffer at 56°C for 15 min ...
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bioRxiv - Neuroscience 2024Quote: ... We loaded 30 µg cytoplasmic and 20 µg nuclear fractions on Any kD Mini-PROTEAN TGX Precast Protein Gels (Bio-Rad) and ran at 100V for 1.5 h ...
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bioRxiv - Molecular Biology 2024Quote: ... and 0.1 vol of DTT 1M was added and the mixture heated at 95°C for 5 min before loading onto Any kD Mini-PROTEAN TGX Stain-Free protein gel (4568124, Bio-Rad) and migration in 1x Tris-Glycine-SDS buffer at 200V ...
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bioRxiv - Bioengineering 2024Quote: ... SDS-PAGE was performed using 4–15% Mini-PROTEAN® TGX™ Precast Protein Gel (#4561084, Bio-Rad Laboratories, Inc, USA). Before and after the laser exposure ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were boiled for 5 min and 20 to 30 μg of protein were separated by SDS–polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes (Bio-Rad). Membranes were blocked in 3% milk in Tris-buffered saline with 0.1% Tween 20 (TBST ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentration was determined using ROTI Nanoquant (Carl Roth GmbH) with a Bio-Rad SmartSpec Plus UV/Vis Spectrophotometer (Bio-Rad). Denatured protein extracts (20 μg ...
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bioRxiv - Molecular Biology 2024Quote: ... and protein signal was detected with the Clarity Western ECL Substrate and imaged on the ChemiDoc MP Imaging System (Bio-Rad).
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bioRxiv - Neuroscience 2024Quote: ... diluted to working concentration and denatured 70°C for 10 minutes prior to loading on 4–20% Mini-PROTEAN TGX Stain-Free Protein Gels (Bio-rad) or 4-12% Bolt Bis-Tris Plus Mini Protein Gels (Invitrogen) ...
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bioRxiv - Molecular Biology 2024Quote: ... Approximately 30-40 μg of protein were loaded onto either 10% in-house acrylamide gels or 4-20% Mini-PROTEAN Tris-Glycine Precast Protein Gels (BioRad, #4561094). Separated proteins were transferred to Odyssey nitrocellulose membranes (LI-COR Biosciences ...
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bioRxiv - Microbiology 2024Quote: ... were incubated with 4 µg of protein for 1h30 min at room temp and then analysed by SDS-PAGE (4-20% resolving gel, Bio-Rad) for cleavage ...
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bioRxiv - Neuroscience 2024Quote: ... sample protein (5-8 µg) and molecular weight ladders were loaded onto a 4–15% Criterion TGX precast gels (Bio-Rad) for gel electrophoresis separation ...
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bioRxiv - Neuroscience 2024Quote: ... 10 µg of total proteins were denatured at 95°C for 5 min in 100 mM dithiothreitol (DTT) and 1x Laemmli buffer (Bio-Rad). Proteins mixtures were separated using a Mini-PROTEAN Tetra Vertical Eletrophoresis Cell for Mini Precast Gels apparatus (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... proteins were eluted from magnetic beads with 1x Laemmli buffer and beta-mercaptoethanol (BME) mixture (Boston biosciences BP111R, Biorad 1610737, Sigma) and boiled at 98°C for 8 min and subsequently examined by SDS-PAGE (immunoblot) ...
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bioRxiv - Cancer Biology 2024Quote: ... Equal amounts of protein (30 µg) were loaded to each well of a mini-PROTEAN 4-20% TGX stain-free precast gels (Bio-Rad). Protein was transferred to nitrocellulose membranes using the TransBlot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Biophysics 2024Quote: ... The gels were run at 130 V until the protein standard lanes were resolved to satisfaction and then stained with Coomassie Brilliant Blue R-250 staining solution from Bio-Rad. Gels were de-stained with the Coomassie Brilliant Blue R-250 de-staining solution.