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Citations for Bio-Rad :
9451 - 9500 of 10000+ citations for Human Protein N terminal asparagine amidohydrolase NTAN1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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VPS13B is localized at the cis-trans Golgi complex interface and is a functional partner of FAM177A1bioRxiv - Cell Biology 2023Quote: ... cDNA was synthesized with the iScript kit (Bio-Rad, #1708841) using 1mg of total RNA ...
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bioRxiv - Immunology 2023Quote: cDNA was synthesized by iScript cDNA Synthesis Kit (Bio-Rad) using an extended 1h amplification ...
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bioRxiv - Immunology 2024Quote: ... Reverse transcription was performed using iScriptTM cDNA Synthesis kits (BioRad) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... complementary DNA (cDNA) prepared (iScriptTM cDNA Synthesis Kit (Bio-Rad)) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and reverse transcribed with iScript cDNA Synthesis kit (Bio-Rad). Partial fragments of each genes were amplified with PCR by using gene-specific primers and cloned into pCR4-TOPO TA vector (Invitrogen ...
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bioRxiv - Pathology 2024Quote: ... 5 μl of 2X iTaq Universal probe kit (Bio-Rad), 300 nM of forward and reverse primers and 200 nM of probe (Table S5 ...
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bioRxiv - Microbiology 2024Quote: ... and qPCR performed using iTaq Universal Probes Kit (Bio-Rad) for SARS-CoV-2 primers52 ...
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bioRxiv - Immunology 2024Quote: ... using SYBR Green PCR Master Mix Kit (Bio-Rad Laboratories) to examine lung mRNA expression of Cxcl1 ...
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bioRxiv - Immunology 2024Quote: ... cDNA synthesis was performed using iScript cDNA synthesis kit (BioRad) according to the manufacturer guidelines ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was synthesized using the iScript cDNA Synthesis Kit (BioRad) and qRT-PCR was performed using PCRBio SyGreen Blue Mix Lo-Rox (PCR Biosystems ...
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bioRxiv - Microbiology 2024Quote: ... using iTaq universal Probes One-step RT-qPCR kit (BioRad) with N2 primers and probes (IDT ...
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bioRxiv - Molecular Biology 2024Quote: ... Germany and cDNA Reverse transcription kit was procured from Biorad. Lipofectmine reagent and Drabkins reagent ...
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bioRxiv - Microbiology 2024Quote: ... and iTaq Universal SYBR Green One-Step kit (Bio-Rad). Primers for RT-qPCR are described in Table 3.
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bioRxiv - Cell Biology 2024Quote: ... The membrane was washed and developed using ECL kit (BioRad). Image analysis and quantification were performed using ImageLab software (BioRad).
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bioRxiv - Plant Biology 2024Quote: ... we extracted RNA using Aurum Total RNA Mini Kit (BioRad), according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... We centrifuged the dialysis contents to remove any insoluble protein and then purified using cation exchange chromatography (BioRad UNOsphere Rapid S column) with an NaCl gradient ...
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bioRxiv - Biophysics 2021Quote: The removal of DDM in the protein solutions was done by fixing the protein concentration at 1 mg/ml and using 30 mg of BioBeads SM2 (Bio-Rad, CA) per mg of protein ...
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bioRxiv - Cell Biology 2020Quote: ... Equivalent amounts of total protein per lysate were loaded onto Criterion XT 4-12% Bis-Tris polyacrylamide gels (Bio-Rad, Hercules, CA), transferred onto PVDF membranes (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... Samples were boiled at 95°C for 5 minutes with Laemmli buffer and 20 μg of each was loaded into a 4-20% Mini-PROTEAN TGX Precast Protein Gel (BioRad, Hercules, CA). Gels were run at 100 V for 30-60 minutes and then transferred to PVDF membranes for 2 hours at 250 mV ...
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bioRxiv - Plant Biology 2020Quote: ... the recombinant protein in the filtrate was further purified by size exclusion chromatography (NGCTM chromatography system with EnrichTM SEC 650 column, BioRad, Munich, Germany) in 50 mM NaH2PO4 /Na2HPO4 ...
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bioRxiv - Developmental Biology 2021Quote: ... by immunoprecipitation of endogenous or endogenously Flag-tagged Piwi (eF-Piwi): The anti-Piwi antibody and Surebeads Protein A Magnetic bead (Bio-Rad, 1614013) were used to immunoprecipitated endogenous Piwi-piRNAs from witld type OSC ...
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bioRxiv - Microbiology 2022Quote: ... Brain homogenates were assayed at neat for all analytes and results were normalised to their total protein concentrations (Bio-Rad, Hercules, California).
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bioRxiv - Neuroscience 2021Quote: ... 20 μg of total cell proteins were run in a 12% SDS-polyacrylamide gel and blotted to the PVDF membrane (Bio-Rad, USA). Membranes were incubated overnight at 4 °C with mature BDNF primary antibody (ThermoFisher OSB00017W ...
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bioRxiv - Neuroscience 2021Quote: ... samples heat denatured and 20 µg of total protein was loaded per lane on 4-20% Tris-HCl polyacrylamide gels (BioRad, California, USA). After transfer to PVDF ...
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bioRxiv - Molecular Biology 2020Quote: ... Biotinylated proteins were then eluted by boiling the magnetic beads in 30 μL 4 χ Laemmli sample buffer (Bio-Rad, Cat.# 1610747) supplemented with 20 mM DTT and 2 mM biotin.
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bioRxiv - Molecular Biology 2020Quote: ... The membrane was incubated with primary antibody and horseradish peroxidase–conjugated secondary antibody, and then proteins were detected using the Pierce™ ECL Western Blotting Substrate (Thermo, #32209) by BIO-RAD ChemiDoc™ XRS+ system ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein was detected using an ECL system (Amersham Pharmacia, Piscataway, NJ, USA) and was analysed using Quantity One software (BIO-RAD, USA).
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bioRxiv - Cell Biology 2022Quote: Protein homogenates (5 or 15 μg/lane) were size fractionated on 4–15% or 15% Criterion TGX gels (Biorad Laboratories, Oslo, Norway) and transferred to 0.45 μM PVDF-membranes (GE Healthcare) ...
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bioRxiv - Neuroscience 2019Quote: ... 5uL of the sample was loaded into 4-6% polyacrylamide gel along with Precision Plus Protein™ Dual Color Standard (#1610374S, Bio-Rad) and was run at 120 V ...
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bioRxiv - Molecular Biology 2019Quote: ... Denatured proteins (20-40 µg) were electrophoresed in 9% SDS-PAGE gels or MiniPROTEAN® TGX™ 4-15% Precast gels (BIORAD), transferred onto a nitrocellulose membrane and probed with specific antibodies ...
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bioRxiv - Biophysics 2019Quote: ... The concentration of the dialyzed protein was estimated using the Quick Start®Bradford Dye Reagent 1X (BIO-RAD Cat. #500-0205). The protein was stored at 4 °C before use.
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bioRxiv - Developmental Biology 2020Quote: ... 1-20μg of tissue protein to be analyzed were combined with equal parts 2X Laemmli sample buffer (Bio-Rad, catalog 161-0737) containing 10% β-mercaptoethanol ...
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bioRxiv - Cancer Biology 2019Quote: ... 40µg of protein from each sample was loaded onto the 4– 15% Mini-PROTEAN® TGX™ Gel (BioRad, Catalog number: 4561083) for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Neuroscience 2019Quote: ... and 50 μg protein per sample were run on an SDS-PAGE gel and transferred to an Immun-Blot PVDF Membrane (Bio-Rad Laboratories) using standard protocols ...
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bioRxiv - Physiology 2019Quote: ... Diluted proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) on a 4-20% gradient Criterion TGX Precast Midi Protein gel (Bio-Rad, CA), and subsequently transferred onto nitrocellulose membranes (Bio-Rad ...
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Virus-mediated transient expression techniques enable genetic modification of Alopecurus myosuroidesbioRxiv - Genetics 2020Quote: Equal volumes of protein were loaded onto pre-cast BioRad Mini-Protean TGX precast Gel 4-20% (15-wells BioRad cat# 4568096). The gels were run according to the manufacturer’s guidelines (110Volts for 3 minutes ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... Proteins were separated on SDS-containing 8 to 12 % polyacrylamide gels and transferred to PVDF membranes (Bio-Rad Laboratories, Inc., Hercules, USA). After staining with primary and secondary antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... 9 µL of each cell lysate and 14 µL of each secreted protein sample were run on a 4–20% Tris-glycine gel (Bio-Rad; 4561094) together with the Precision Plus Protein Dual Color Standards molecular weight marker (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: Electrophoretic analyses of viral proteins and equine formulations were performed in 4-20% SDS-PAGE gel Mini-PROTEAN TGX (BioRad; California, USA) and 7.5% SDS- polyacrylamide gels (SDS-PAGE) ...
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bioRxiv - Biochemistry 2020Quote: ... protein was eluted from the beads by boiling for 5 minutes at 95°C in Laemmli Sample Buffer (BioRad, catalog #161-0747). Proteins were stored at −80°C until western blotting.
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bioRxiv - Bioengineering 2021Quote: ... Produced protein was sequentially purified by affinity chromatography using Ni-NTA agarose beads and fast protein liquid chromatography (FPLC, NGC Quest 10 Chromatography system, Biorad, Hercules, CA). Protein fractions detected at λ = 280 were collected ...
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bioRxiv - Bioengineering 2020Quote: ... Tumors were homogenized and lysed using RIPA buffer and the total protein concentrations were determined using DC assay reagent (Bio-Rad Laboratories). Cell lysates (50 μg ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were then loaded onto 4-15% Criterion™ TGX™ Precast Midi Protein Gels (12 + 2 wells, 45 μl) (Bio-Rad) and subjected to SDS-PAGE at 200 V for ~45 min in 10× Tris/Glycine/SDS running buffer (Bio-Rad) ...
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bioRxiv - Neuroscience 2020Quote: ... A gel slice corresponding to 100 to 150 kd was isolated (based on size markers in an adjacent lane; Precision Plus Protein™ Dual Color Standards from Bio-Rad). The gel slice was transferred to a D-Tube Dialyzer Midi Tube (EMD-Millipore) ...
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bioRxiv - Cell Biology 2021Quote: ... 10 µg of the cleared lysate were separated by SDS-PAGE using 4%-15% Mini-PROTEAN TGX Precast Protein gels (Bio-Rad, #4561085) and blotted onto a Amersham Protran Premium Western blotting nitrocellulose membrane (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... Equal amounts of protein were run on 6% SDS-PAGE gels and blotted onto nitrocellulose using a semi-dry Trans-Blot (Bio-Rad, 1703940). Blots were incubated overnight at 4°C with a rabbit anti-myosin 18A polyclonal antibody that recognizes the C-terminal 18 residues of both myosin 18Aα and 18Aβ (Bruun et al. ...
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bioRxiv - Cell Biology 2019Quote: ... The protein extract was first separated by loading equal amounts of protein on 4-20 % Mini-PROTEAN® TGX™ Gel SDS–PAGE gradient gels (456-1096, Biorad) and then transferred to the nitrocellulose membrane with Trans-Blot Turbo Transfer Pack (170-4159 ...
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bioRxiv - Physiology 2021Quote: ... 10 μg of total protein for each sample was separated by SDS-PAGE and transferred to Immun-Blot PVDF membrane (Bio-Rad Laboratories). Subsequently ...
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bioRxiv - Biochemistry 2020Quote: ... The clarified supernatant protein extracts were subjected to SDS-PAGE and proteins were transferred to a nitrocellulose membrane according to manufacturer’s instructions (Bio-RAD, Hercules, CA). Protein blots were first probed with GL2-specific antibody ...
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bioRxiv - Cell Biology 2021Quote: ... 3–8% Tris-acetate gels (Life Technologies. Proteins were transferred to nitrocellulose membrane by semi-dry transfer (Trans-Blot Turbo transfer system; Bio-Rad) or ...