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Citations for Bio-Rad :
9401 - 9450 of 10000+ citations for Hepatitis E ORF2 protein mouse Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... Growth media was exchanged after 24 hours and cells were checked for protein expression using a fluorescent cell imager (Bio-Rad). Cells were harvested after another 24 hours in cold PBS before purification ...
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bioRxiv - Biophysics 2024Quote: ... The reaction was then centrifuged at 14,000rpm for 5 minutes and the Ire1-LD concentrations in supernatant and pellet (after washing three times in HMK buffer) were measured using the Bradford protein assay (Bio-Rad) with bovine serum albumin as a standard ...
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bioRxiv - Cancer Biology 2024Quote: ... 30 µg of protein lysate were then run per lane on a 10% polyacrylamide gel (Bio-Rad Laboratories, Hercules, CA, USA) and transferred to a PVDF membrane (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2024Quote: ... 30-50 μg of total protein was boiled at 99°C for 5 minutes with the addition of Laemmli Sample Buffer (1610747, Bio-Rad) containing 1% 2-Mercaptoethanol in a total volume of 60-80 μl ...
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bioRxiv - Microbiology 2024Quote: ... The samples were then treated according to the instruction of the beads and the protein complexes were then eluted by incubating at 99°C for 5 minutes with Laemmli Sample Buffer (1610747, Bio-Rad).
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bioRxiv - Synthetic Biology 2024Quote: ... the maximum amount of protein that could be loaded to keep protein mass constant across all samples on a gel) was loaded onto a 4–15% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad) and run at 50 V for 10 min followed by 100 V for at least 1 h ...
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bioRxiv - Physiology 2024Quote: ... Protein obtained was run on SDS-PAGE on precast polyacrylamide gel (Mini-PROTEAN TGX, 4%-20%; Bio-Rad Laboratories, Hercules, CA) and transferred to a polyvinylidene difluoride membrane via a semi-wet transfer equipment (Trans-Blot Turbo Transfer System ...
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bioRxiv - Neuroscience 2024Quote: ... They were resolved by SDS–PAGE using a Bio-rad system and 4–15% Mini-PROTEAN TGX Precast Protein Gels (#4561086, Bio-rad) and transferred to nitrocellulose membranes (pore size 0.45 µm ...
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bioRxiv - Molecular Biology 2024Quote: ... Equal amounts of protein lysates (20 µg) were separated in 10% SDS-PAGE gels and transferred to PVDF membranes (Bio-Rad). The membranes were incubated with specific primary antibodies (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were denatured at 95°C for 10 min and run on 4-20% Criterion TGX Precast Midi Protein gels (Bio-Rad). Resolved protein was transferred to nitrocellulose using Trans-Blot turbo transfer packs ...
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bioRxiv - Neuroscience 2024Quote: ... The proteins were eluted from beads by adding the loading buffer (4X Laemmli sample buffer [Bio-Rad] with 20 mM dithiothreitol) followed by a 10 min incubation at 95°C ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were centrifuged for 30 min at 20,000 x g at 4 °C and protein concentrations of the supernatants were measured with standard Bradford assay (Bio-Rad Laboratories). 25 or 50 µg of proteins were diluted in Laemmli buffer 3× (188 mM Tris-HCl ...
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bioRxiv - Neuroscience 2024Quote: Biotinylated proteins were eluted from streptavidin beads by the addition of 20 μL of elution buffer (2X Laemmli sample buffer [Bio-Rad] ...
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bioRxiv - Cell Biology 2023Quote: ... proteins incubated with Mal-B were electrotransferred to PVDF membranes using a Trans-blot SD semi-dry transfer cell (Bio-Rad). Biotin incorporation was assessed by incubating blots with HRP-streptavidin (1:1000 v/v ...
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bioRxiv - Microbiology 2024Quote: ... Proteins of interest (FtsZ, FtsN and MinD) were transferred to nitrocellulose membranes using a semi-dry Transfer-Blot apparatus (Bio-Rad). The Nitrocellulose membranes were then blocked in 5% (w/v ...
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bioRxiv - Immunology 2024Quote: ... the proteins were transferred to a polyvinylidene difluoride membrane (110V, 1h, 4°C) using a Mini Trans-Blot cell apparatus (Bio-Rad). Non-specific binding sites were blocked using PBS/Tween 20 (0.05% Tween 20 ...
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bioRxiv - Immunology 2024Quote: ... Equal amounts of pre-cleared lysate samples were separated in 8 – 16% Mini protean-TGX stain free protein gel (Bio-Rad) and the gel was transferred to a Trans-Blot Turbo Mini-PVDF membrane (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: 10 µL of the normalized protein samples were loaded onto a Mini-PROTEAN TGX Any kD (Bio-Rad Catalog #456-9036) SDS-polyacrylamide gel and electrophoresed in 1X SDS-PAGE Running Buffer (25 mM Tris ...
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bioRxiv - Molecular Biology 2023Quote: ... and 30 µg of total protein extract were loaded on 10% acrylamide-bis-acrylamide gels or 4-20% Mini-PROTEAN TGX Precast Gels (Bio-Rad). Proteins were separated by migration at 135 V in 1X Tris-Glycine-SDS buffer (Euromedex) ...
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bioRxiv - Molecular Biology 2023Quote: ... resin with immunoprecipitated proteins were resuspended in SDS-loading buffer and electrophoresed on a 4-20% TGX stain-free gel (Bio-Rad) for 10 min ...
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bioRxiv - Cell Biology 2023Quote: ... Equal amounts of protein lysates (20 µg) were separated in 10% SDS-PAGE gels and transferred to PVDF membranes (Bio-Rad). The membranes were incubated with specific primary antibodies (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins (10–30 μg) were run on a 10% SDS–polyacrylamide gels and then transferred to a nitrocellulose membrane (Biorad®). The membranes were blocked in 5% lowfat milk-PBS-tween solution and then incubated overnight at 4 °C with primary antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... 200 μg aliquots of mitochondria were flash-frozen in liquid nitrogen after measuring the protein concentration by a Bradford assay (Bio-Rad) and stored at −80°C.
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bioRxiv - Cell Biology 2023Quote: ... For each protein of interest 10 µg of sample was loaded in a 4-15% Tris-Glycine gel (Bio-Rad, 4568083). The gel was transfered onto PVDF membrane (GenScript ...
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bioRxiv - Cell Biology 2023Quote: ... 40-80 μg protein was separated on SDS-PAGE gels and transferred to polyvinylidene difluoride (PVDF) membranes (#162–0177; Bio-Rad). Membranes were blocked in phosphate buffered saline (PBS)-Tween with 5% non-fat dry milk ...
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bioRxiv - Cell Biology 2023Quote: ... 30 μg of total protein per sample was subjected to reducing and denaturing SDS-PAGE prior to Western transfer onto nitrocellulose membrane (Bio-Rad). After blocking with 5% skim milk in PBS containing 0.1% (v/v ...
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bioRxiv - Immunology 2023Quote: ... Samples normalized to equal amounts were denatured in Laemmli’s buffer at 95°C for 10 min and resolved by SDS-PAGE using Criterion TM TGX TM Midi protein gels (Bio-Rad). Resolved proteins were transferred onto 0.2μM PVDF membrane by the Criterion TM Blotter (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: ... USA) and the relative optical density of protein bands was quantified using Quantity One Software from Bio-Rad (Hercules, CA, USA).
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bioRxiv - Evolutionary Biology 2024Quote: ... 50 µg of the membrane protein eluate fraction quantified by BSA were diluted to 32 μl in lysis buffer containing loading Laemmli buffer (Bio-Rad) supplemented with 10% 2-mercaptoethanol ...
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bioRxiv - Cancer Biology 2024Quote: ... The insoluble material was separated at 13,000 rpm for 30 minutes at 4 °C and total protein concentration was measured on supernatants using Bradford reagent (Bio-Rad). Protein extracts were denatured with NuPAGE®LDS (Invitrogen ...
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bioRxiv - Synthetic Biology 2023Quote: ... The purity and size of the proteins were validated on an SDS-PAGE gel (Mini-PROTEAN TGX and Mini-TETRA cell, Bio-Rad). Purified proteins were stored at 4° C.
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bioRxiv - Molecular Biology 2023Quote: ... proteins were transferred from the unstained gel to polyvinylidene fluoride membranes using the Trans-Blot turbo transfer system (Bio-Rad Laboratories). An iBind™ Flex system (Invitrogen/ThermoFischer ...
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bioRxiv - Synthetic Biology 2023Quote: ... 10 μL of each sample was separated on an Any kD™ Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad). Proteins were electrophoretically transferred to a PVDF membrane using a Trans-Blot® Turbo™ Transfer System (BioRad ...
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bioRxiv - Cell Biology 2023Quote: ... Approximately equal loads of extracted protein were subjected to SDS-PAGE and transferred to a 0.2 µm nitrocellulose membrane (Bio-Rad 1620112). Transferred proteins were then stained with Ponceau S Solution (Sigma-Aldrich P3504) ...
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bioRxiv - Microbiology 2023Quote: ... 12 % SDS-PA gels were fixed with fixation solution (40 % ethanol, 10 % acetic acid) o/n and stained in Flamingo fluorescent protein dye (Bio-Rad) for up to 6 h and imaged with the ChemiDoc (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: ... The blots were washed and the labelled protein bands were visualized by using Pierce ECL reagent and a ChemiDoc XRS+ system (Bio-Rad).
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bioRxiv - Cancer Biology 2022Quote: 40 µg of proteins were separated using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) on a 10% acrylamide gel (BIO-RAD) and transferred to nitrocellulose membrane by semi-dry blotting using the Trans-Blot Semi-Dry Transfer Cell (Bio-Rad ...
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bioRxiv - Genetics 2023Quote: ... The rabbit polyclonal antiserum was raised against purified recombinant Thp1 protein and subsequently affinity purified as Thp1p coated affinity beads (Affi-Gel 10 beads, Bio-Rad) according to standard procedures ...
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bioRxiv - Molecular Biology 2023Quote: ... 40 μg of protein extract was loaded into a 10% TGX Stain-Free FastCast Acrylamide Gel (Bio-Rad, Hercules, CA, USA) or SDS polyacrylamide 15% running gel in the case of histone analysis ...
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bioRxiv - Molecular Biology 2023Quote: ... and light units were normalized by the total protein concentrations of the corresponding WCEs determined using the Bradford reagent (BioRad, 5000006).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... before being loaded on a SDS-PAGE gel (4–20% Criterion™ TGX Stain-Free™ Protein Gel, Bio-Rad #5678095). Western blot was then performed as described in the previous section.
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bioRxiv - Plant Biology 2023Quote: ... The proteins were then transferred to polyvinylidene difluoride membranes using NuPAGE Transfer Buffer using a Trans-Blot Turbo Transfer System (Bio-Rad) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were denatured by boiling for 10 minutes in the DTT containing sample buffer and separated by 4-20% Mini-PROTEIN TGX gels (BIO-RAD) and then transferred to the 0.2 μm PVDF membrane (BIO-RAD) ...
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bioRxiv - Biochemistry 2023Quote: ... Cyt-b5 and other protein band intensities were determined using the volume contour tool Image Lab (Bio-Rad; Hercules, CA, USA) or ImageJ software ...
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bioRxiv - Cell Biology 2023Quote: ... Equal amounts of total protein samples (40-50 µg) were resolved by polyacrylamide gels using self-made or pre-cast gels (Bio-Rad). Proteins were electroblotted on nitrocellulose membranes (Cytiva ...
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bioRxiv - Physiology 2023Quote: ... The subsequent protein pellet was solubilized in 50 µL Laemmli sample buffer and 20 µg protein was run in a 12.5% SDS-Page gel (BioRad Criterion system). The gels were fixed and stained with Coomassie blue (GelCode blue ...
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bioRxiv - Neuroscience 2023Quote: ... 10 µg of proteins in Laemmli buffer and protein standard were loaded on 4%-15% Criterion TGX Stain-Free protein gel in running buffer TGS 1XL(all from Bio-Rad) and transferred to a 0.2Lµm PVDF membrane with the Trans-Blot Turbo RTA Midi Transfer Kit (Bio-Rad) ...
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bioRxiv - Immunology 2023Quote: Purified recombinant His-tagged proteins were diluted to 0.5µg/ml in either Laemmli sample buffer alone (Bio-Rad, Hercules, CA, USA) or containing 50 mM dithiothreitol (DTT ...
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bioRxiv - Neuroscience 2023Quote: ... proteins (20 μg per lane) were separated by SDS–polyacrylamide gel electrophoresis and transferred to a polyvinylidene difluoride membrane (Bio-Rad). Membranes were blocked in 4% milk in TBST [0.01 M tris (pH7.4) ...
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bioRxiv - Cell Biology 2023Quote: All the protein samples were separated by sodium dodecyl sulfate polyacrylamide (SDS)-PAGE and were transferred to nitrocellulose membrane (Bio-Rad laboratories ...