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Citations for Bio-Rad :
9301 - 9350 of 9412 citations for Recombinant Human FGF12 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... Proteins extracts were boiled at 95°C and then separated onto Mini-PROTEAN ® TGX™ precast protein SDS-PAGE (4-15% gradient) gels (BIO-RAD, #4561083). Proteins were transferred onto polyvinylidene fluoride (PVDF ...
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bioRxiv - Molecular Biology 2021Quote: ... The secondary antibodies were detected via chemiluminescence using Westar Supernova (XLS3,0020, Cyanogen) and the protein bands were visualised in a ChemiDoc XRS+ imager (Bio-rad with image Lab software).
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bioRxiv - Cancer Biology 2021Quote: Westerns were performed by loading 30-50ug of total protein per well into 15 well polyacrylamide gels (Bio-Rad, California, USA, Cat #456-1086) and run for 30min at 200V ...
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bioRxiv - Cancer Biology 2021Quote: Separation of 20 μg or 50 μg protein from cell lysate was performed on a 10% Mini-PROTEAN® TGX™ gel (BioRad, Portland, ME) and subsequently transferred onto Immunoblot PVDF 0.45μm membranes (BioRad ...
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bioRxiv - Cell Biology 2021Quote: ... Fluorescent dye-conjugated secondary antibodies were used at 1:3000 and the proteins were detected using ChemiDoc MP Imaging System (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Microbiology 2022Quote: ... Resulting lipopolysaccharides were separated using gradient 4–20% sodium dodecyl sulfate□polyacrylamide gel (4–20% Mini□PROTEAN® TGX™ Precast Protein Gel, BioRad, Hercules, USA) electrophoresis (SDS□PAGE ...
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bioRxiv - Bioengineering 2019Quote: ... Western blot analysis was performed using 30 to 50 µg proteins run on Mini-PROTEAN TGX precast gels (Bio-Rad cat. nr. 456-1094). αRANBP9 primary antibody was purchased from Abcam (cat ...
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bioRxiv - Neuroscience 2021Quote: ... to a final concentration of 10 mM and shielded from light for 30 minutes.We performed an automated detergent compatible protein assay (DCA) (Bio-Rad, Hercules, CA, Cat. #5000111), normalization (to 1 μg/μL ...
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bioRxiv - Microbiology 2021Quote: ... The native molecular mass of 12β-HSDH was determined by comparing its elution volume to that of Gel Filtration Standard proteins (Bio-Rad, Hercules, CA, USA): thyroglobulin ...
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bioRxiv - Bioengineering 2021Quote: Ramos B cells were retrovirally transduced with a construct encoding spike protein tagged with the fluorophore mScarlet at the C-terminus and sorted by FACS (Bio-Rad S3e Cell Sorter). For the experiment ...
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ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: Proteins were transferred from gels to PVDF membranes using a Trans-Blot® Turbo™ Transfer System and associated accessories (Bio-Rad Laboratories, Inc.). Membranes were probed with anti-Strep-II tag and anti-CB2 antibodies according to manufacturer instructions.
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bioRxiv - Plant Biology 2019Quote: ... equal protein amounts were loaded onto 7.5% polyacrylamide gels (Mini-PROTEAN®TGX Stain-Free™ Precast Gels, Bio-Rad Laboratories, Hercules, CA, USA) and fractionated by gel electrophoresis under reducing conditions and then blotted onto PVDF membranes (Immun-Blot®PVDF Membrane ...
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bioRxiv - Microbiology 2020Quote: ... as per the manufacturer’s running conditions then activated for 5 min under UV to determine total protein (Bio-Rad ChemiDoc XR, Image Lab Software). Proteins were transferred to PVDF membranes (Bio-Rad) ...
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bioRxiv - Cancer Biology 2019Quote: ... 50ug protein was separated on 10% ECL gels (GE, Pittsburgh, PA) and transferred to nitrocellulose using the Trans-Blot® System (Bio-Rad, Hercules, CA). Antibodies are listed in Supplemental Table S10.
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bioRxiv - Bioengineering 2019Quote: ... the protein bands were visualized upon few minutes of incubation of the membranes with Bio-Rad Clarity Western ECL Substrate (Bio-Rad#1705061, Cressier, Switzerland). Blots were imaged using ChemiDoc imaging system for chemiluminescence assays with 0.1 s exposure ...
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bioRxiv - Immunology 2020Quote: ... A chemiluminescence image of the ABCC4 blot was taken in tandem with an image of total protein loading using Bio-Rad Image Lab software (Bio-Rad Laboratories, Mississauga, Canada).
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bioRxiv - Synthetic Biology 2021Quote: ... diluted 1:200 in 10 mM Tris-HCl pH 7.5 and 9 μL loaded (along with 1 μL 1 M DTT and 10 μL 2X Laemmli Buffer) into an Any kD™ Mini-PROTEAN® TGX™ Precast Protein Gel (Bio-rad). For Western blot ...
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bioRxiv - Plant Biology 2021Quote: ... Protein quantities were measured by Bradford assays according to the manual provided with the Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad Laboratories; Hercules, USA). Proteins were immunoprecipitated by 50 µl Anti-HA MicroBeads (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2021Quote: ... total protein was extracted and 15-20µg from each sample was separated on a 10% Mini-PROTEAN® TGX™ Precast Protein Gel (Biorad, Hercules, CA., USA) adjacent to the Li-COR Chameleon ladder (Li-Cor ...
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bioRxiv - Genetics 2020Quote: ... diluted samples were boiled for 5min at 95 °C and subsequently separated on a 4-15% Criterion™ TGX Stain-Free™ Protein Gel (Bio-Rad Laboratories) and ran in a Criterion™ Cell geltank (Bio-Rad Laboratories ...
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bioRxiv - Biochemistry 2020Quote: ... The eluted proteins or whole cell lysates were separated by SDS-polyacrylamide gel electrophoresis on a 4-20% gradient gel (Bio-Rad Laboratories, Hercules, CA) and transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Biochemistry 2021Quote: ... Protein extracts (50 μg of protein) in β-mercaptoethanol containing SDS sample buffer were separated in 4% to 12% gradient SDS-polyacrylamide gels (Bio-Rad #456-8094) and transferred to nitrocellulose membranes (Bio-Rad #170-4271 ...
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bioRxiv - Molecular Biology 2021Quote: ... 25 µg of each protein mixture was separated by SDS-PAGE (polyacrylamide gel electrophoresis) on a Criterion XT 4-12% Bis-Tris gel (Biorad Laboratories cat. No. 3450124). Proteins were transferred to a PVDF membrane (Merck Millipore cat ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... following which they were subjected to separation via sodium dodecyl sulfate-polyacrylamide gel electrophoresis using 8% or 10% gels and the Mini-Protein II apparatus (Bio-Rad, CA, United States). Protein bands were electroblotted onto the polyvinylidene difluoride (PVDF ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins were transferred on a PVDF membrane according to the manufacturer’s protocol (Trans-Blot® Turbo™ Rapid Transfer System, BIORAD, Les-Ulis, France) using a small protein transfer program ...
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bioRxiv - Cancer Biology 2021Quote: ... 20 to 30 μg of lysate protein was subjected to electrophoresis on a 4–15% gradient mini-Protean TGX gel (Bio-rad, Hercules, CA, USA). It was then transferred to a membrane using a semidry method with a Trans-Blot Turbo Transfer System (Bio-rad) ...
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bioRxiv - Immunology 2021Quote: We mixed 500 μg of extracted cell lysates and 100 μL of patients’ sera with 100 μL of SureBeads™ Protein G Magnetic Beads (Bio-Rad, Hercules, CA) and added phosphate-buffered saline containing 0.2% of Tween 20 (PBS-T ...
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bioRxiv - Immunology 2021Quote: ... 20 to 30 µg of lysate protein was subjected to electrophoresis on a 4–15% gradient mini-Protean TGX gel (Bio-Rad, Hercules, CA, USA). It was then transferred to a membrane using a semidry method with a Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Cell Biology 2021Quote: ... Protein extracts (50 µg of protein) in β-mercaptoethanol containing SDS sample buffer were separated in 4% to 12% gradient SDS-polyacrylamide gels (Bio-Rad #456-8094) and transferred to nitrocellulose membranes (Bio-Rad #170-4271 ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 μl of serial dilutions of standard protein and samples were mixed with 250 μl of Bradford reagent (500-0205, BIO-RAD Quick Start™). Samples were incubated 10 minutes at room temperature and measured at OD595 using an ELISA plate reader (TECAN Infinite M200 Pro) ...
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bioRxiv - Biochemistry 2022Quote: Protein overexpression and purification were analysed via 10% SDS-PAGE and imaged via ChemiDoc™ MP Imaging System (Bio-Rad; Fig. S1 and S4). Immunoblotting was performed to confirm purification results with primary anti-6xHis polyclonal mouse antibody (Beyotime® ...
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bioRxiv - Cell Biology 2022Quote: ... The radio-labelled proteins were then separated with SDS-PAGE on a 4-12% Criterion XT Bis-Tris gels (Bio-Rad, Hercules, CA, USA) in MES buffer (Bio-Rad) ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies (Supplemental Table 1) were incubated for 1 hour at RT and protein bands were visualized using a ChemiDoc XRS+ (Bio-Rad Laboratories, Hercules, USA).
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bioRxiv - Genomics 2022Quote: ... Protein concentration was quantified with the Quick Start Bovine Serum Albumin Bradford assay according to the manufacturer’s protocol (Bio-Rad Laboratories, Inc., Hercules, CA) and using an Eppendorf Biophotometer 6131.
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bioRxiv - Physiology 2022Quote: ... Samples were prepared for electrophoresis by denaturing 20μg of protein per sample in XT Sample Buffer (Bio-Rad, Hercules, CA, USA, Cat# 161-0791) and XT Reducing agent (Bio-Rad Cat# 3161-0792 ...
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bioRxiv - Neuroscience 2023Quote: ... A 5 μL aliquot of the aSyn protein not incubated or incubated for different periods under aggregating conditions was transferred to a nitrocellulose membrane (Bio-rad®; 0.45 μm). The sample was then blocked with OdysseyTM blocking buffer for 60 min at 22° C ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... An equal amount of total protein was resolved by a 10% sodium dodecyl sulfate polyacrylamide gel (SDS-PAGE) and transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). Membranes were blocked and incubated with either anti Phospho-(Ser/Thr ...
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bioRxiv - Neuroscience 2022Quote: ... were loaded onto 4-12% Criterion XT precast gels and proteins were separated by SDS-PAGE under reducing conditions (Bio-Rad Laboratories, Hercules, CA). The proteins were then electroblotted onto a 0.45 μm nitrocellulose membrane ...
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bioRxiv - Molecular Biology 2022Quote: 20µg of protein extracts or 10% Co-IP samples were resolved on 10-12% SDS–PAGE and proteins were transferred to PVDF membranes using a semi dry Western blot system (Bio-Rad Trans-Blot SD). Membranes were blocked in 5% non-fat milk in PBS-T (1x PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... Equal amounts of protein were resolved by sodium dodecyl sulfate-PAGE and transferred to a polyvinylidene fluoride membrane (Bio-Rad Laboratories, Hercules, CA, USA). Blots were probed with the indicated primary antibodies listed in Extended Data Table 4 ...
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bioRxiv - Genomics 2022Quote: ... XCell II Blot module was used for wet electrophoretic transfer of proteins from SDS-PAGE gel to nitrocellulose membrane (Biorad, Cat # 1620145, 0.45 μm) in Tris-Glycine Electroblotting buffer (National Diagnostic ...
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bioRxiv - Immunology 2022Quote: The purity and quality of the proteins were characterized at all stages of the purification process using 4%-20% SDS–PAGE (Bio-Rad, Catalog number 4561096) and 3%-12% Bis-Tris gel (ThermoFisher ...
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bioRxiv - Microbiology 2024Quote: ... purity was checked by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in a 12% polyacrylamide gel using the Bio-Rad Protein Assay (Bio-Rad, Hercules, CA, USA), according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... The total protein concentration of pooled saliva samples was measured to be approximately 20 mg/mL using the Bradford assay kit (Bio-Rad, Hercules, CA, USA) and bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2023Quote: ... Equal amounts of proteins (20 μg) were separated by SDS-PAGE and then transferred onto nitrocellulose membranes (ref 1704271, Bio-Rad, Hercules, California, USA). Membranes were incubated in blocking buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 μl of serial dilutions of standard protein and samples were mixed with 250 μl of Bradford reagent (500-0205, BIO-RAD Quick Start™).
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bioRxiv - Developmental Biology 2024Quote: Protein lysates (40 µg per lane) from uteri or oviducts were loaded on a 4-15% SDS-PAGE gel (Bio-Rad, Hercules, CA, USA), separated in 1X Tris-Glycine Buffer (Bio-Rad) ...
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bioRxiv - Immunology 2024Quote: ... 20 to 30 µg of lysate protein was subjected to electrophoresis on a 4–20% gradient mini-Protean TGX gel (Bio-rad, Hercules, CA, USA). It was then transferred to a PVDF membrane using a semidry method with a Trans-Blot Turbo Transfer System (Bio-rad) ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 to 30 µg of lysate protein was subjected to SDS-PAGE electrophoresis on a 4–15% gradient mini-Protean TGX gel (Bio-rad, Hercules, CA, USA). It was then transferred to a membrane using a semidry method with a Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... After gel migration proteins were transferred to Amersham nitrocellulose Protran Western blotting membranes (GE10600001) using semi-dry transfer (SEMI DRY BLOT APPARATUS, Bio-Rad, cat. no.1703940). Blots were blocked in TBS-T and 5 % milk and probed with relevant antibodies overnight ...