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Citations for Bio-Rad :
9151 - 9200 of 10000+ citations for Monkey Caveolin 1 CAV1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: 2 μg of total protein derived from meiotic or post-meiotic germ cell populations was loaded onto a Mini-PROTEAN TGX gel (Bio-Rad, 456-8093) and separated by SDS-PAGE for 1 hour at 150V ...
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bioRxiv - Cell Biology 2019Quote: ... Equal amounts of proteins were fractionated via 8 ∼ 14% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and the separated proteins were transferred onto polyvinylidene difluoride (PVDF) membrane using a Trans-blot apparatus (Bio-Rad, Hercules, CA). The PVDF membranes were then sequentially subject to blocking ...
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bioRxiv - Cell Biology 2021Quote: ... Forty µg of denaturated proteins were subjected to a Western blot analysis after 4–15 % MiniPROTEAN® TGX StainFree™ gel electrophoresis (Bio-Rad) followed by probing antibodies against TLR4 or β-Actin (TLR4:1:400 ...
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bioRxiv - Microbiology 2022Quote: ... 10 μg of protein was loaded from each sample into separate wells of a 4-15% polyacrylamide gel (Bio-Rad Laboratories, 456-8084). Electrophoresis was performed in 1x Tris/Glycine Buffer (Bio-Rad Laboratories ...
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bioRxiv - Cancer Biology 2022Quote: ... Lysates (32 μg proteins/lane) were loaded to and separated on 10% SDS-PAGE gels and electrophoretically transferred to PVDF membrane (1620264, Bio-Rad, Hercules, CA). The membranes were blocked in 5% BSA/TBST (0.1% Tween-20 ...
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bioRxiv - Cell Biology 2022Quote: ... 20-30 μg of protein extract was loaded onto 4 to 20% SDS-polyacrylamide gel electrophoresis (SDS-PAGE) gels (Bio-Rad, Hercules, CA) and transferred to Immobilon-P PVDF membranes (Bio-Rad ...
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bioRxiv - Genetics 2022Quote: ... Approximately 20 μg of each sample were loaded on a 4-20% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad cat no. 4561094). Proteins were transferred to a Nitrocellulose Membrane (Bio-Rad cat no ...
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bioRxiv - Genetics 2022Quote: ... Duplicate samples run on the same gels were used for total protein staining with QC Colloidal Coomassie Stain (Bio-Rad cat no. 1610803).
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bioRxiv - Microbiology 2022Quote: ... and mixed 1:1 with 2X Laemmli buffer followed by SDS-PAGE using 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels and staining with Coomassie Brilliant Blue R-250 (Bio-Rad) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... full-length S protein samples were prepared from cell pellets and resolved in 4-15% Mini-Protean TGX gel (Bio-Rad, Hercules, CA) and transferred onto PVDF membranes ...
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bioRxiv - Molecular Biology 2023Quote: ... An equal amount of total protein (20 – 50 μ per sample) was separated by SDS-PAGE and transferred to a PVDF membrane (Bio-Rad, 1620177) for blotting.
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bioRxiv - Neuroscience 2022Quote: ... 30 μg of protein was heated in a 70°C water bath for 10 min prior to loading into into a 4-15% Criterion TGX precast Midi protein gel (Bio-Rad, Cat#5671094) for SDS-PAGE followed by wet transfer to a nitrocellulose membrane ...
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bioRxiv - Microbiology 2022Quote: ... steady-state levels of protein were detected by Coomassie-staining of the gel and scanning employing the Gel Doc XR+ System (Bio-Rad, USA, Hercules). Signals were quantified with ImageJ 1.53e (29).
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bioRxiv - Immunology 2022Quote: ... Both anti-IL-9 and the isotype antibody were purified from the culture supernatant of hybridoma cells using the Proteus protein purification spin kit (Bio-Rad, Hercules, CA). Anti-IL-13 is an IgG1 antibody (55 ...
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bioRxiv - Molecular Biology 2022Quote: ... the protein bands were detected by LumiQ HRP Substrate solution (Share-bio, SSA003) and imaged with ChemiDoc™MP imaging system (Bio-Rad, USA)
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bioRxiv - Cancer Biology 2024Quote: Total protein concentrations were determined against a standard curve of BSA in a 96-well plate using the DC™ Protein assay (Bio-Rad Laboratories). Proteins were separated on reducing 10% NuPAGE™ Novex™ Bis-Tris Protein Gels and transferred onto PVDF membranes ...
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bioRxiv - Bioengineering 2023Quote: ... and boiled at 95 °C for 5 min before loading and running in 4-15% Mini-PROTEAN TGX Precast Protein Gels (Bio-Rad, Cat# 4561083) with Precision Plus Protein Dual Color Standards (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... 10-20 µl of the cell lysates were separated on 4-15% Mini-PROTEAN® TGX™ Stain-FreeTM Protein Gels from Bio-Rad and transferred to methanol-soaked polyvinylidene difluoride (PVDF ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein lysates from each cell line were analysed on multiple gels (four per cell line) with Precision Plus Protein Dual Color Standard (1610394, Bio-Rad, Hercules, CA) as ladder and blotted to membranes to separately probe for phosphorylated and total forms of the same proteins ...
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bioRxiv - Systems Biology 2024Quote: 25 μg of RIPA or 5ug of lipid raft protein lysate was loaded onto a dot blot apparatus (Bio-Rad, Hercules, CA, USA) and were filtered through 0.45μm PVDF for 2 hours by gravity filtration ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting pellets were resuspended in resuspension buffer (RB; Table S7) and proteins levels were quantified by Lowry (Bio-Rad #5000113, #5000114, #5000114). 600 µg aliquots of each sample were pre-heated at 95°C for 5 min ...
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bioRxiv - Pathology 2024Quote: ... 15 µg of proteins were resolved on Criterion TGX Stain-Free 4-15%,18 and 26-well gel (Bio-Rad Laboratories, #5678084, #5678085) in a Criterion large format electrophoresis cell (Bio-Rad Laboratories ...
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bioRxiv - Physiology 2024Quote: ... Then we separated proteins into 4-15% gradient SDS-PAGE gels and transferred them to PVDF immuno-blot membranes (Bio-Rad, Hercules, CA). The primary antibodies S6 (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... The relative amount of transferred protein per sample was determined after Ponceau S staining by a GS-800 densitometer (Bio-Rad, Hercules, USA) and used as loading control ...
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bioRxiv - Cell Biology 2023Quote: ... Total nascent peptides were detected by in-gel imaging at 647 µm and total protein was detected by far red epi imaging after staining with 0.1% Coomassie brilliant blue (BioRad ChemiDoc MP Imaging System). For detection of nascent insulin and tubulin ...
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bioRxiv - Neuroscience 2023Quote: ... the membranes were washed three times with TBS-Tween 0.2 % for 15 min and immunoreactive proteins were detected with enhanced chemiluminescence reagents according to the manufacturer instructions (Bio-Rad, Hercules, CA USA). Signals were captured with the ChemiDoc system (Bio-Rad ...
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bioRxiv - Pathology 2023Quote: ... The total protein concentrations of each liver samples were determine using a standard curve generated with BSA at different known concentration using the Quick Start™ Bradford Protein Assay (Bio-Rad, U.S.A.). On the basis of the measure protein cencetrions ...
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bioRxiv - Cell Biology 2023Quote: ... then centrifuged at 13,000 rpm for 30 min at 4 °C and the resulting supernatants were subjected to Bradford protein assay (Bio-Rad, Hercules, California, USA) for measuring total protein concentration ...
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bioRxiv - Microbiology 2023Quote: ... 12 μl of purified recombinant orthologous proteins at a concentration of 250 nM were loaded onto a TGX FastCast 12% stain-free gel (Bio-Rad, Feldkirchen, Germany). Electrophoresis was conducted at 220 V for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... 20 µL of protein lysate was then loaded into 4–20% Criterion™ TGX Stain-Free™ precast gels (Bio-Rad, Cat. #5678094). Proteins were separated at 200V for 45 min and then transferred to a low-fluorescence PVDF membrane at 25V for 30 min using the Trans-Blot Turbo Transfer System and Kit (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... ECL signals and total protein loading amounts were measured with a Bio-Rad ChemiDoc MP imaging system (Bio-Rad Laboratories Inc., Hercules, CA). The acquired images were analyzed with Bio-Rad ImageLab software version 6.1 (Bio-Rad Laboratories).
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bioRxiv - Cancer Biology 2023Quote: ... Electrophoresis and blotting of protein extracts were performed using mini-PROTEAN tetra handcast and trans-blot turbo transfer systems (Bio-Rad, Hercules, CA).
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bioRxiv - Immunology 2023Quote: ... Same protein amounts were loaded onto 4-12% NuPAGE Gels and protein was transferred to PVDF membranes by using Trans-Blot® Turbo™ Transfer System (Biorad#17001917). Membrane was blocked with 5% milk-TBS ...
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bioRxiv - Cell Biology 2023Quote: ... 20-40 μg protein were separated by SDS-PAGE on 4-15% Mini- PROTEAN® TGX Stain-Free™ Gels (Bio-Rad®). and transferred onto PVDF membranes using Trans-Blot Turbo RTA Mini LF PVDF Transfer Kit (Bio-Rad®) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the concentrated AmGSTD1 protein was injected onto a Hydroxyapatite column (HA) connected to the NGC chromatography System (Bio-Rad Laboratories, Hercules, CA, USA). A gradient of 5 mM NaPi and 5 mM HEPES ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein contents were visualized on a ChemiDoc MP system using the ImageLab software for quantification (both from Bio-Rad Laboratories GmbH, Munich, Germany). For the demonstration of equal loading ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were then transferred onto 0.2 µm nitrocellulose membranes using Trans-Blot® Turbo™ Mini Nitrocellulose Transfer Packs (Bio-Rad, Cat #: 1704158EDU) on a Trans-Blot Turbo TM Transfer System (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: Some of the affinity chromatography-purified proteins were run through a Superdex200 Increase 10/300 GL column integrated in an NGC chromatography system (Bio-Rad, CA, USA) to obtain a profile of the content in different species (aggregates ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal quantity of the protein was resolved in a 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels and transferred to an Immun-Blot PVDF Membrane (Bio-Rad) for antibody incubation ...
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bioRxiv - Neuroscience 2023Quote: ... heated for 5 min at 95°C and loaded onto 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad, Germany). Proteins were transferred on nitrocellulose membranes and membranes were blocked with 5% BSA in PBS-Tween ...
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bioRxiv - Molecular Biology 2023Quote: 20-25μg of protein lysate with 4x Laemmli buffer (cat# 1610747; Bio-Rad Laboratories was separated on 4-15% Mini-PROTEAN TGX Precast Protein Gels (cat# 4561086; Bio-Rad Laboratories) and transferred to Immun-Blot PVDF Membranes for Protein Blotting (cat# 1620177 ...
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bioRxiv - Physiology 2023Quote: ... 25 µg of total protein was loaded into precast 10 % BIO-RAD Mini-PROTEAN® TGX Stain-FreeTM SDS-polyacrylamide gel (Bio-RAD Laboratories), then electrophoretically transferred to 0.2 µm nitrocellulose membrane (Trans-Blot® TurboTM Transfer Pack ...
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bioRxiv - Cell Biology 2023Quote: ... and the protein transferred to 0.45 mm polyvinylidene difluoride (PVDF) membrane using the Trans-Blot Turbo transfer system (BioRad Laboratories, Hatfield, PA, USA).
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bioRxiv - Biochemistry 2023Quote: ... Eluted proteins were first analysed using SDS-polyacrylamide gel electrophoresis (SDS-PAGE) using TGX Stain-Free precast gels (Bio-Rad, Hercules, Ca, USA). The molecular weight of the recombinant proteins was estimated using Invitrogen™ BenchMark™ Pre-stained Protein Ladder (Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were centrifuged at 16,000Xg for 30 min at 4°C then protein concentrations were determined by Quick Start™ Bradford 1x Dye Reagent (Bio-Rad #5000205). For each sample ...
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bioRxiv - Genetics 2023Quote: ... Co-IP reactions were rotated overnight at 4°C with 100 µl of SureBeads Protein G Magnetic Beads (BioRad; Catalog# 1614013; Hercules, CA). The bead lysates were washed five times in the co-IP buffer using a DynaMag Magnet (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: ... Protein concentrations of selected cytokines and chemokines were measured in supernatants of macrophages using a BioPlex MAGPIX Multiplex Reader (BIO-RAD, United States) according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... The centrifuged samples (10 μL sample volume) were loaded into a 4-15% gradient Mini-Protean TGX precast protein gel (Biorad, CA, Cat # 4561086) and run at 140 V ...
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bioRxiv - Biophysics 2023Quote: ... Cleavage of LD650-labelled GPΔTM was verified by in-gel fluorescence imaging of uncleaved and cleaved protein on a 4-20% polyacrylamide using a ChemiDocTM MP imaging system (Bio-Rad, Hercules, CA) followed by Coomassie staining ...
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Post-stroke rapamycin treatment improves post-recanalization cerebral blood flow and outcome in ratsbioRxiv - Neuroscience 2023Quote: ... 50 µg of protein was denatured (95°C for 5 minutes) using lysis and Laemmli sample buffer (161-0737, Bio-Rad, with DTT). Samples were separated on a 10 % gradient gel (Criterion TGX Precast Gel ...