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Citations for Bio-Rad :
9051 - 9100 of 10000+ citations for Monkey Caveolin 1 CAV1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... boiled for 5 min at 95°C and resolved by SDS-PAGE in 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (#4561086, BioRad). After electrophoresis ...
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bioRxiv - Cancer Biology 2024Quote: ... A molecular weight marker was added onto each gel for reference (Precision Plus Protein Dual Color, #1610374, Bio-Rad, Hercules, CA, USA). Transfer on PVDF membranes was done using Pierce Western blot transfer buffer (#35040 ...
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bioRxiv - Cell Biology 2024Quote: ... Then an equal amount of proteins were separated on a polyacrylamide gel with pre-cast gradient (Mini-PROTEAN® Precast Gels, acrylamide 4% −20%, Bio-Rad) and transferred to nitrocellulose membrane (Trans-Blot® Turbo ™ Midi Nitrocellulose Transfer Packs ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Proteins from total and bound fractions were separated by 7.5 % SDS-PAGE and transferred to polyvinylidene difluoride membranes (Bio-Rad, Hercules, CA). To reduce the background signal ...
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bioRxiv - Plant Biology 2024Quote: ... The protein bands containing PSII supercomplexes were cut out and eluted by a Model 422 Electro-Eluter (Bio-Rad, Hercules, CA, USA). The relative amount of PSII supercomplexes were quantified by dot immunoblot analysis with D1 antibody (Agrisera ...
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bioRxiv - Cancer Biology 2024Quote: ... Thirty micrograms of each protein sample were separated by SDS-PAGE and transferred onto a nitrocellulose membrane using the Transblot Turbo Transfer System (Bio-Rad, 1704150EDU). Non-specific binding sites on the membranes were blocked with 5% BSA in TBS-Tween 0.1% for 60 min ...
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The HUSH epigenetic repressor complex silences PML nuclear bodies-associated HSV-1 quiescent genomesbioRxiv - Microbiology 2024Quote: ... Protein extracts were boiled for 10 minutes and then loaded on polyacrylamide gels (TGX Stain-Free FastCast gels (Bio-Rad, 1610181/1610183) or home-made gels ...
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bioRxiv - Microbiology 2024Quote: ... 20 μg of total protein was loaded onto each lane of SDS-PAGE on a 10% acrylamide gel (BIO-RAD cat. #4561034) and run 45 min at 60 V followed by 45 min at 110 V ...
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bioRxiv - Microbiology 2024Quote: ... 8 μL of lysate were loaded in each lane and resolved on a 7.5% Mini-PROTEAN® TGX™ precast protein gel (Biorad 4561025) for PBP1a and PBP1b samples ...
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bioRxiv - Immunology 2024Quote: ... Electrophoresis was conducted in MES buffer at 100-120 V for 40 min with protein standards (NEB, P7119S, or Bio-Rad, #1610373). Proteins were transferred to nitrocellulose membrane and membranes blocked in 5% milk powder/PBS overnight at 4 °C ...
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bioRxiv - Physiology 2024Quote: ... Proteins were separated by SDS-PAGE and transferred to PVDF membranes using a semi-dry transfer cell (Bio-Rad, Hercules, CA, USA). Membranes were blocked with 5% non-fat dry milk in TBS-Tween for 1 h at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... The indicated total protein amount was loaded on SDS-PAGE gels (4-20% Mini-protean® TGX™ precast gels, [Bio-Rad] ...
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bioRxiv - Cell Biology 2024Quote: ... After running the samples by an SDS polyacrylamide gradient gel (4–20% Criterion™ TGX™ Precast Midi Protein Gel, Bio-Rad) and transferring them to a PVDF membrane (350mA/1h) ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were denatured at 95°C for 10 mins and subjected to SDS-PAGE in a 4–12% Criterion™ XT Bis-Tris Protein Gel (Bio-Rad) using XT MES Running Buffer (Bio-Rad) ...
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bioRxiv - Plant Biology 2024Quote: ... the immunoprecipitated and input proteins were separated on SDS–PAGE gels and transferred onto PVDF membranes via a Trans-Blot Turbo Transfer System (Bio-Rad, USA). After blocking the membranes with a solution of 5% skim milk prepared in PBST with 0.1% Tween 20 ...
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bioRxiv - Immunology 2024Quote: ... A total of 80 µg of protein homogenate were separated by SDS-PAGE and transferred onto PVDF membranes (Bio-Rad, Hercules, CA). Membranes were blocked with fat-free milk powder (5% w/v ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies were anti-mouse (Bio-Rad, #170-6516, dilution 1:1000 or 1:2000) and anti-rabbit (Bio-Rad ...
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bioRxiv - Microbiology 2019Quote: ... 1:5000 and 1:10000 dilutions of the libraries in triplicate on iQ5 (Bio-Rad) and CFX96 Touch qPCR machines ...
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bioRxiv - Plant Biology 2021Quote: ... 0.05% SDS) and a semi dry transfer cell (Bio-Rad, 1 mA/cm2, 1 h). Membrane was blocked with protein free blocking solution T20 (Pierce ...
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bioRxiv - Microbiology 2019Quote: ... was diluted 1:5000 in 1% (w/v) casein blocking buffer (Bio-Rad, 161-0782). Blots were incubated at room temperature for 2 h ...
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bioRxiv - Bioengineering 2021Quote: ... samples were mixed 1:1 with 2x Laemmli sample buffer with 2-mercaptoethanol (Bio-Rad) and boiled for 10min ...
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bioRxiv - Microbiology 2021Quote: ... 1× iTaq Universal SYBR Green Supermix or 1× iTaq Universal Probe Supermix (Bio-Rad, USA), 300 nM each of forward primer (late RT product ...
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bioRxiv - Physiology 2021Quote: ... Sections were incubated with F4/80 antibody (Clone Cl:A3-1, Bio-Rad, MCA497R, 1:100) followed by biotinylated antibody (Vector ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were incubated with the HRP-conjugated secondary antibodies (1:2500-1:10000, Bio-Rad) for 1h at RT ...
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bioRxiv - Immunology 2024Quote: Samples were prepared in 1 x Laemmli buffer with or without 1 x dithiothreitol (Biorad). Samples were then heated for 10 min at 95 °C and loaded onto a precast NuPAGE™ 4-12 % Bis-Tris polyacrylamide gel in NuPAGE™ MES SDS running buffer (Thermo Fisher Scientific) ...
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bioRxiv - Physiology 2023Quote: ... prepared at a 1:1 ratio and acquired using the ChemiDoc MP System (Bio-Rad).
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bioRxiv - Genetics 2022Quote: Samples taken from prior assays were mixed 1:1 with Laemmli Sample Buffer (Bio-Rad) with 10% 2-mercaptoethanol and vortexed to ensure adequate mixing ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was diluted 1:1 with Laemmli Sample Buffer (1610737, BIO-RAD, Hercules, CA) and heated to 80°C for 30 min.
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bioRxiv - Neuroscience 2019Quote: ... or BIC (20 μM) were added to the culture and cells were harvested with a 1:1 mixture of 2X Laemmeli buffer (BioRad, 1610737, 1:20 beta-mercaptoethanol) and radioimmunoprecipitation assay (RIPA ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were incubated with secondary antibodies for 1 hour at room temperature (anti-rabbit or anti-mouse, 1:10.000, Bio-Rad, anti-goat, 1:7500, Pierce). Antibody binding was detected by chemiluminescence(Clarity kit ...
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bioRxiv - Microbiology 2021Quote: ... followed by secondary antibodies (1:4000 donkey anti-goat, Abcam; 1:4000 goat anti-mouse, Biorad). Immunoblots were developed using SuperSignal West Pico PLUS Chemiluminescent Substrate (ThermoFisher).
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bioRxiv - Microbiology 2021Quote: ... 20 uL cell suspension was diluted 1:1 with 0.4% Trypan Blue solution (Bio-Rad #1450021) and cells were counted by hemocytometer (RPE-1 cells ...
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bioRxiv - Neuroscience 2021Quote: ... Each compartment of each sample was diluted 1:1 in Laemlli Sample Buffer (BioRad, Hercules, CA) mixed with 350mM DTT (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... The following antibodies and dilutions were used: F4/80 1:100 (C1:A3-1, Bio-RAD), αSMA 1:100 (AS-29553 ...
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bioRxiv - Microbiology 2022Quote: ... 100 μl fresh urine and several dilutions (1/10; 1/100) were plated on UriSelect4 (Biorad) and KFS (Biolife italiana ...
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bioRxiv - Plant Biology 2023Quote: ... 0.3% (w/v) CHAPS containing protease inhibitors (1 tablet Biorad/10ml ; 1/100 v/v PMSF). Phosphatase inhibitors were also added in L3 and L4 experiments ...
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bioRxiv - Physiology 2022Quote: ... monoclonal rat anti-F4/80 antibody (dilution 1:200) (clone Cl:A3-1, MCA497G, Biorad Laboratories, Germany) After multiple washes ...
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bioRxiv - Cell Biology 2020Quote: ... The protein samples were mixed with LDS sample loading buffer at 4X concentration followed by addition of beta-mercaptoethanol (5%) (Bio-Rad, Hercules, CA). The resultant mixture was denatured at 95°C for 9 min ...
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bioRxiv - Cell Biology 2020Quote: ... 20 mg of total protein was separated by SDS-PAGE on polyacrylamide gels (10%) and transferred to a polyvinylidene fluoride membrane (Bio-Rad Laboratories, Inc.). The membrane was probed with antibodies against Flag (#F7425 ...
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bioRxiv - Cell Biology 2020Quote: ... The purity and integrity of the proteins was evaluated for each batch by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) using 4-15% gradient gels (Mini-PROTEAN® TGX® Precast Protein Gels, Bio-Rad) with a Precision Plus Protein® Kaleidoscope® (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... Twenty micrograms of protein were loaded on 4-15% gradient TGX Stain-Free™ Protein Gels and transferred to Immun-Blot® LF PVDF membrane (Bio-Rad). The membranes were blocked with 1X TBS with 0.05% Tween 20 and 5% skim milk powder for 2 hours at 25°C ...
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bioRxiv - Neuroscience 2021Quote: ... Around 10-15 μl (around 20 μg of protein) were resolved by SDS-PAGE polyacrylamide gel electrophoresis using 4-20 % precast gradient gels (Bio-Rad, 456-1096), and proteins were electrophoretically transfected onto nitrocellulose membranes (GE Healthcare) ...
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bioRxiv - Developmental Biology 2020Quote: ... The blot was washed and incubated with SuperSignal™West Femto (Thermo Fischer Scientific, USA) to reveal the proteins with ChemiDoc™ Touch Imaging System (BioRad, USA).
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bioRxiv - Genomics 2020Quote: ... Nuclei were resuspended in Laemmli buffer and loaded into 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad Cat #4561093). SDS-PAGE and protein blotting were performed according to (Dawe et al ...
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bioRxiv - Molecular Biology 2022Quote: HEK293 cells were transiently transfected in 24-well plates for RNA analysis and in 10 cm plates for protein extractions and IP respectively using a TransFectinTM reagent (Bio-Rad, Hercules, California). 4 h after transfection ...
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bioRxiv - Physiology 2022Quote: ... and protein content assessed by BCA assay (Pierce) before 15 ug protein lysates were separated on a TGX Stain-Free™ criterion gel (Bio-Rad, Sweden). Following which ...
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bioRxiv - Neuroscience 2021Quote: ... 40 μg proteins were loaded and separated by SDS-PAGE gels (10%) electrophoretically and transferred to polyvinylidene difluoride membrane (Bio-Rad Incorporation, 10026933). Blocking was performed by adding 5% BSA for 60 min at room temperature on the shaker ...
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bioRxiv - Molecular Biology 2021Quote: ... The boiled proteins were separated on 4% to 15% SDS-PAGE gradient gels and transferred to LF PVDF membrane (Bio-Rad, Cat #1620260). The membranes were blocked in 5% skim milk in TBST (20 mM-Tris ...
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bioRxiv - Cell Biology 2022Quote: ... the intestine-specific intestinal fatty acid binding protein promoter (-2.3kb fabp2 -also called ifabp-) was amplified by high fidelity PCR (iProof_ High-Fidelity DNA Polymerase; Bio-Rad, Hercules, CA, USA) from p5E–2.3ifabp plasmid (kindly gifted by J ...
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bioRxiv - Cell Biology 2019Quote: ... lysates were centrifuged at 16,000 x g to pellet cellular debris and protein concentration was determined by BCA quantification assay (Bio-Rad, Hercules, CA, USA). For each sample ...