Labshake search
Citations for Bio-Rad :
9351 - 9400 of 10000+ citations for Monkey Caveolin 1 CAV1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... After gel migration proteins were transferred to Amersham nitrocellulose Protran Western blotting membranes (GE10600001) using semi-dry transfer (SEMI DRY BLOT APPARATUS, Bio-Rad, cat. no.1703940). Blots were blocked in TBS-T and 5 % milk and probed with relevant antibodies overnight ...
-
bioRxiv - Biophysics 2023Quote: ... the protein concentrations were determined by image analysis of SDS-PAGE with 4-20% Mini-PROTEAN® TGX Stain-Free™ Protein Gels (Bio-Rad, 4568096) followed by Coomassie Brilliant Blue staining using the following concentration standard ...
-
LRP1 mediates leptin transport by coupling with the short-form leptin receptor in the choroid plexusbioRxiv - Neuroscience 2023Quote: Cell or tissue lysates (10–30 μg protein) or immunoprecipitated samples were resolved by SDS–PAGE and transferred to nitrocellulose membranes (Bio-Rad Laboratories, Hercules, CA). The membranes were incubated with antibodies against leptin (Cat# ...
-
bioRxiv - Biochemistry 2023Quote: ... Total protein concentrations of the cell extracts were determined using the Bio-Rad Protein Assay Kit according to the manufacturer’s instructions (Bio-Rad Laboratories, Hercules, CA, USA) using BSA as standard.
-
bioRxiv - Biochemistry 2023Quote: ... and for Western blotting by electro-transferring proteins from gels to PVDF membranes (0.2 μm pores) using a Trans Blot Turbo system (Bio-Rad Laboratories S.r.l., Segrate, Italy) according to manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cellular proteins were resolved using sodium dodecyl sulfate-polyacrylamide gel electrophoresis before being electroblotted onto polyvinylidene fluoride membranes (#1704156, Bio-Rad Laboratories, Hercules, CA). The membranes were incubated with the following primary antibodies for 60 min at room temperature ...
-
bioRxiv - Biochemistry 2023Quote: The recombinant protein of each vaccine candidate was purified under denaturing conditions using a biologic duoflow™ chromatography system (Bio-Rad Laboratories, Hercules, USA). A HisTrap™ HP column (GE Healthcare Life Sciences ...
-
bioRxiv - Molecular Biology 2023Quote: ... The protein was transferred to PVDF membrane (Amersham 10600022) using semi-dry transfer apparatus using the ‘mixed MW’ setting (Bio-Rad trans blot turbo). Membranes were blocked for 1 hour in 5% powdered milk in TBS-T ...
-
bioRxiv - Plant Biology 2023Quote: ... Seed storage proteins were visualized from 100 µg of seed material on a 12% SDS-PAGE compared to a Precision Plus Dual Color Protein Standard (Bio-Rad, Hercules, CA, USA). Gels were stained for 1 hr using a 0.1% Coomassie Brilliant Blue ...
-
bioRxiv - Biochemistry 2023Quote: ... The proteins were stained with coomassie blue R250 and PMMoV-I coat protein was quantified using a densitometer and the quantity one software (Bio-Rad, Hercules, CA, USA).
-
bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of proteins (20 μg) were separated by SDS-PAGE and then transferred onto nitrocellulose membranes (ref 1704271, Bio-Rad, Hercules, California, USA). Membranes were incubated in blocking buffer ...
-
bioRxiv - Cell Biology 2023Quote: ... 20 µg of each cell protein lysate sample was separated using a hand-cast 5-15% gradient Tris glycine gel (Bio-Rad, Protocol Bulletin 6201) using a custom made 25-well comb.
-
bioRxiv - Biochemistry 2023Quote: ... Protein yields for both nitrogenase component fractions were determined using the Quick Start™ Bradford 1x Dye Reagent (Bio-Rad Laboratories, Inc., Hercules, USA) according to the instructions by the manufacturer and purity of both protein fractions was analysed via sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) ...
-
bioRxiv - Molecular Biology 2023Quote: ... 25ug of protein was used for Western blot run in 4-20% gradient gel (Criterion TGX Stain-free, #5678094, Bio-Rad, Hercules, California, USA). Proteins were transferred to nitrocellulose membrane in Turboblotter (Bio-Rad ...
-
bioRxiv - Neuroscience 2023Quote: ... 40ug of each sample was denatured at 95°C for 5 minutes and loaded in 4–20% Mini-PROTEAN® TGX™ Precast protein gels (Bio-Rad; SA) with a gel lane including a Dual Xtra standard protein ladder (Bio-Rad ...
-
bioRxiv - Immunology 2023Quote: ... Western blotting was performed by electrophoresis of 20 µg of proteins on Mini-PROTEAN or Criterion TGX Precast Gels (Bio-Rad, Hercules, CA, USA), followed by electrotransfer to Amersham™ Protran® nitrocellulose membrane (Millipore Sigma) ...
-
bioRxiv - Microbiology 2024Quote: ... A 40 µg of protein of CFS samples and 10 ng of purified rGdhA were resolved in a 4-20% SDS-PAGE (Bio-Rad, Hercules, CA; # 4561094) and immunoblotted with affinity purified anti-rabbit rGdhA antibody (0.3 µg/mL ...
-
bioRxiv - Neuroscience 2024Quote: ... at 95°C for 10 mins before being loaded into 4-20% Mini-PROTEAN® TGXTM Precast Protein Gels (15 wells, 15uL; Bio-Rad, CA, USA) and subject to electrophoresis on a Bio-Rad Mini-PROTEAN® Tetra Cell system (180 V ...
-
bioRxiv - Microbiology 2024Quote: ... 10*106 virions of IMP-1088 or DMSO released purified viruses were lysed in 4x Laemmli protein sample buffer (BIO-RAD, Cat. no. 1610747). Samples were electrophoresed on 4-20% precast polyacrylamide gel (BIO-RAD ...
-
bioRxiv - Neuroscience 2024Quote: ... Equal amounts of protein (20 μg/lane) were resolved in 10% Bis-Tris Criterion™ XT Precast Gels and XT MOPS running buffer (BioRad, Cat# 1610799, USA) at 120 volts for 1.5 h using Criterion™ vertical electrophoresis cell (BioRad ...
-
bioRxiv - Neuroscience 2024Quote: ... 30µg of protein homogenates were separated by SDS-PAGE on a 4-12% gradient Bis-Tris gel (Bio-Rad, Hercules, CA, Criterion XT 3450125) in XT MES buffer (Bio-Rad ...
-
bioRxiv - Animal Behavior and Cognition 2024Quote: Whole cell lysates containing SDS buffer were homogenized with a 27-G syringe and resolved on 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad, 4568096) or 6/10/12% home-made polyacrylamide gels and transferred to nitrocellulose membranes (Fisher Scientific ...
-
bioRxiv - Molecular Biology 2024Quote: ... Protein samples (10 µg of protein per well) were separated on SDS-polyacrylamide gels (CriterionTM TGXTM Precast Gels, 4-15%; Bio-Rad, Hercules, CA, USA) using a Laemmli buffer system and then semidry transferred to nitrocellulose membranes by a Trans-Blot Turbo Transfer System (Bio-Rad ...
-
bioRxiv - Microbiology 2024Quote: ... The collected protein samples were electrophoresed on 10% SDS-polyacrylamide gel (SDS-PAGE) and transferred to the polyvinylidene difluoride (PVDF) membrane (Bio-Rad, Cat. No. 1620177). After blocking with 5% bovine serum albumin (BSA ...
-
Programmed ER fragmentation drives selective ER inheritance and degradation in budding yeast meiosisbioRxiv - Cell Biology 2021Quote: ... Samples were then incubated at RT for 1 hr in primary anti-tubulin antibody (Bio-Rad, 1:200) in PBS-BSA (5 mM potassium phosphate ...
-
bioRxiv - Cancer Biology 2020Quote: ... cells were resuspended in agarose insert buffer and mixed 1:1 with 2% low-melting agarose (Bio-Rad). Gel inserts were solidified at 4°C for overnight and were stored in agarose insert buffer until analysis ...
-
bioRxiv - Microbiology 2020Quote: ... for 1 h at 0.4 V cm−1 in a Mini-PROTEAN® Tetra Cell (Bio-Rad, CAD). His-tagged proteins were detected using monoclonal anti-polyHis-HRP conjugate (Sigma-Aldrich ...
-
bioRxiv - Epidemiology 2019Quote: ... such as HIV-1 Western Blot (Genetic Systems HIV-1 Western Blot, Bio-Rad Laboratories, Redmond, Washington, US) and/or an immunofluorescent antibody (IFA ...
-
bioRxiv - Neuroscience 2019Quote: ... goat anti-rat (1:1000; Alexa Fluor 568, #A11077) and V5-tag:AlexaFluor-647 (1:200; Bio-Rad MCA1360A647).
-
bioRxiv - Neuroscience 2020Quote: ... the blots were incubated for 1 hour with horseradish peroxidase– conjugated secondary antibody (1:1000; Bio-Rad, USA). Finally ...
-
bioRxiv - Microbiology 2019Quote: ... and BB2-epitope tag (1:4,000) followed by HRP-conjugated goat anti-rabbit antibody (1:4,000; Bio-Rad). Membrane blocking ...
-
bioRxiv - Plant Biology 2021Quote: ... membrane was incubated 1 min in 1 ml enzyme solution (Clarity™ Western ECL, BIORAD, Les-Ulis, France) and 1 ml Luminol/enhancer solution (Clarity™ Western ECL ...
-
bioRxiv - Cell Biology 2020Quote: ... resuspended in 1 × PBS and spread on the surface of 1% low melting point agarose gel (Bio-Rad) prepared in conditioned medium ...
-
bioRxiv - Neuroscience 2022Quote: ... membranes were incubated with secondary antibody for 1 hour at room temperature (anti-mouse, 1:10.000, Bio-Rad). Antibody binding was detected by chemiluminescence (Clarity kit ...
-
bioRxiv - Neuroscience 2023Quote: ... sections were incubated in IBA1 (Wako; anti-rabbit 1:1000) and CD68 (Bio-Rad; anti-mouse 1:150) and kept at 4_°C overnight with slow agitation ...
-
bioRxiv - Biochemistry 2022Quote: ... in 1% (v/v) acetic acid] for 1 min and photographed using Gel Doc XR system (Bio-Rad).
-
bioRxiv - Biochemistry 2023Quote: ... or double stained with polyclonal anti-M antiserum (1:1000) and monoclonal anti-F (1:500, BIO-RAD) antibodies ...
-
bioRxiv - Microbiology 2023Quote: ... 1:1 ratios of cells to trypan blue were mixed and loaded on dual chambered slides (Biorad # 1450003). Total cell numbers ...
-
bioRxiv - Physiology 2023Quote: ... HRP-conjugated secondary antibodies were applied (Immun-Star Goat Anti-Mouse, 1:20,000, 1705047 and Immun-Star Goat Anti-Rabbit, 1:20,000, 1705046, Biorad), and then activated with Clarity Max western ECL (Bio-Rad) ...
-
bioRxiv - Molecular Biology 2023Quote: ... a 160kD glycoprotein expressed by murine macrophages 235 (1:100, Bio-Rad clone C1:A3-1, Cat. MCA497) and rabbit anti-laminin (1:50 ...
-
bioRxiv - Developmental Biology 2024Quote: ... 1 g SDS in 1 L distilled water) using the Mini-PROTEAN® Tetra electrophoresis system (Bio-Rad). Then ...
-
bioRxiv - Plant Biology 2024Quote: ... followed by a 1 h incubation with anti-mouse IgG HRP-conjugated secondary antibodies (Bio-Rad, 1:3000) at room temperature.
-
bioRxiv - Evolutionary Biology 2024Quote: ... We used 1 μL diluted cDNA (1:3 in ddH2O) and iTaq Universal SYBR Green Supermix (Bio-rad) in the Bio-rad CFX96 real-time PCR detection system for qPCR experiments ...
-
bioRxiv - Microbiology 2020Quote: ... Sheep and-TGN46 (Biorad:AHP500G, IF-1:200); Mouse anti-Actin (Sigma Aldrich ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... and CD3 (BioRad #MCA1477, 1:100 60’). These antibodies recognize murine epitopes ...
-
bioRxiv - Molecular Biology 2020Quote: ... anti-tubulin (1:2000) (Bio-Rad, MCA77G), anti-PIE-1(P4G5 ...
-
bioRxiv - Cell Biology 2020Quote: ... mouse anti-WIPI2 (1:100, Bio-Rad); rabbit anti-ATG9 (1:100 ...
-
bioRxiv - Immunology 2019Quote: ... CD206 (Bio-rad, rat, MR5D3, 1:1000), ER-TR7 (Abcam ...
-
bioRxiv - Neuroscience 2019Quote: ... rat anti-MBP (Biorad, MCA409S, 1:100), goat anti-Gfap (Abcam ...
-
bioRxiv - Neuroscience 2019Quote: ... anti-F8/40 (1:100, BioRad, MCA497R), anti-Collagen 4(1:400 ...