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Citations for Bio-Rad :
8951 - 9000 of 10000+ citations for Monkey Caveolin 1 CAV1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... the membrane was incubated with peroxidase-conjugated goat anti-mouse IgG and proteins were detected using a chemiluminescence detection kit (Super-Signal West Pico, Pierce) and a ChemiDoc™ MP system (Bio-Rad).
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bioRxiv - Cancer Biology 2020Quote: ... 50μg of proteins were electrophoresed in 8% or 12% polyacrylamide gel and then transferred to PVDF membranes (Bio-Rad Laboratory, CA, USA) overnight ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were separated by polyacrylamide gel electrophoresis (PAGE) on a 10% mini-PROTEAN® TGX™ precast gel (Bio-Rad, CA, USA) at 140 V and 300 mA for 1 h ...
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bioRxiv - Cancer Biology 2022Quote: ... prestained protein ladder were resolved on 10% acrylamide gels by SDS-polyacrylamide gel electrophoresis and electrophoretically transferred to nitrocellulose membrane (BioRad Cat. # 1620112) at 100 volts for 90 min.
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bioRxiv - Cancer Biology 2022Quote: ... An equal amount of protein from control and each experiment was loaded onto 4-20% SDS-PAGE gels (Mini-PROTEAN TGX Gels, Bio-Rad, USA) and transferred to the nitrocellulose membranes (Amersham™ Protran® 0.45 μM ...
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bioRxiv - Bioengineering 2022Quote: ... Samples (10-15ug protein/lane) were run by electrophoresis on pre-cast SDS-Page Stain Free Gels (Bio-Rad, Hercules, CA, USA). Separated proteins were transferred to low fluorescence PVDF membranes using the TransBlot Turbo (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... Quantification of band intensity were performed under non-saturated conditions and normalized to the Pgk1p protein using the Image Lab Software (Bio-Rad, Inc.).
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bioRxiv - Cell Biology 2022Quote: ... Samples and PageRulerTM Prestained Protein Ladder (ThermoScientific) were loaded onto Mini-PROTEAN® precast gradient (4-20 %) polyacrylamide gels (Bio-Rad Laboratories) and proteins were separated in an electric field of 90-120 V for varying time spans in SDS Running Buffer (25 mM Tris-base ...
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bioRxiv - Cancer Biology 2022Quote: Whole cell extracts of NB4 and HT93 were prepared using urea lysis buffer and 30-60μg total protein was loaded on a denaturing 12%-polyacrylamide gel self-cast gel (Bio-Rad Laboratories, CA). Proteins were transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Cell Biology 2022Quote: Equal concentration of proteins from EV samples (10μg) were loaded onto precast SDS-polyacrylamide gels (BioRad, Mini Protean TGX Cat# 456-1086) and transferred to Immobilon-E PVDF Transfer membrane (Sigma ...
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bioRxiv - Immunology 2022Quote: ... aliquots of frozen mEVs were thawed at 37°C and Bio-Rad Protein Assay (Bradford 1976) was performed following manufacturer’s instructions (500-0006 Bio-Rad. München, Germany). Samples were diluted with filtered PBS 1:50 and bovine serum albumin (BioRad Laboratories ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentration of the supernatant was determined using the BCA protein assay (Pierce™) and 15 µg was loaded on the gel (3450124, Bio-rad), which was next turbo transferred on nitrocellulose membrane (1704159 ...
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bioRxiv - Immunology 2022Quote: ... Peak fractions pooled and the concentrations of conjugated mi3 particles were determined using a Bio-Rad Protein Assay (Bio-Rad, Hercules, CA). Conjugated nanoparticles were characterized by electron microscopy imaging and SEC as shown in Figure S1C-E ...
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bioRxiv - Molecular Biology 2022Quote: ... Either 50 μg or 25 μg (puromycin blots only) of total protein was separated by SDS-PAGE and transferred to a PVDF membrane (Bio-Rad, 1620177) for blotting ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the whole cell membrane pellets were resuspended in buffer and protein concentration was estimated by dye binding (Coomassie Brilliant Blue R-250; Bio-Rad, USA). The binding reaction was carried out in a final volume of 0.1 ml of Na+-containing buffer (20 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2023Quote: ... Equal amounts of protein extract (20–30 μg) were resolved by SDS-PAGE (4–15% gradient precast TGX polyacrylamide gel, Bio-Rad Laboratories), followed by standard western blot procedure ...
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bioRxiv - Molecular Biology 2023Quote: ... The high molecular weight protein GCN1 was transferred using the Trans-Blot Turbo Transfer System (Bio-Rad, Cat# 1704150 and Cat# 1704158). Subsequently ...
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bioRxiv - Plant Biology 2023Quote: Equal amounts (10 µg) of nuclear proteins from different samples were separated using SDS– PAGE and electrotransferred to a nitrocellulose membrane (BioRad, 162-0115) for 1.5 h at constant 400 mA at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins (30 μg/lane) were separated on an SDS-polyacrylamide gel and transferred to a polyvinylidene difluoride (PVDF) membrane (Bio-Rad Laboratories). The membrane was immunoblotted for CSF1R (#MAB3291 ...
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bioRxiv - Neuroscience 2022Quote: ... Protein samples (∼15 μg) were loaded and separated on 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE, Bio-Rad, Hercules, CA), and transferred to polyvinilidene difluoride membranes (PVDF ...
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bioRxiv - Microbiology 2022Quote: ... Laemmli buffer was added to samples.10 μL of samples were loaded onto 12 % Mini-P ROTEAN® TGX™ Precast Protein Gels (Biorad). Silver staining was carried out at room temperature for 1 h and gels were developed for 2 to 3 mins ...
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bioRxiv - Neuroscience 2022Quote: ... ∼20 μg protein were run on a 4-12Bis-Tris Criterion XT Precast Gel (#3450123, Bio-Rad (Bio-Rad, Hercules, CA, USA) next to the Precision Plus Protein Dual Color Standard (catalog#161-0374 ...
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bioRxiv - Physiology 2022Quote: ... Proteins were visualized by the enhanced chemiluminescence technique (ECL, Amersham, Munich, Germany) and developed using ChemiDoc™ Imaging System (BioRad, Feldkirchen, Germany).
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bioRxiv - Biophysics 2022Quote: ... Samples were centrifuged for 2 min at 12,000 x g before being separated on 8–16% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad) at 160 V for 60 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein pellet was resuspended in UTC buffer (8 M urea, 2 M thiourea, 4% CHAPS) and quantified using RC DC™ Protein Assay Kit (Bio-Rad). Sample with 80 µg proteins resuspended in rehydration buffer (8 M urea ...
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bioRxiv - Microbiology 2024Quote: ... elution fractions with the highest protein concentrations were pooled and checked by SDS-PAGE (Laemmli, 1970) using a 4–20% Mini-PROTEAN gradient gel (BioRad, Munich, Germany). Final protein concentration of the pooled elution fraction was determined with the Pierce BCA Protein Assay Kit (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... the protein(s) in the gel were transferred to a PVDF membrane using a Mini-PROTEAN Tetra Cell (Bio-Rad, United States). After blocking the membrane with 3% BSA at 4°C overnight ...
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bioRxiv - Neuroscience 2023Quote: ... The eluted protein was subjected to an additional purification step using fast protein liquid chromatography (FPLC) and a Superdex 200 Increase 10/300 GL column (Cytiva, 28990944) in Chelex 100-treated (Bio-Rad, 1422832) dialysis buffer (25 mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2024Quote: ... The presence of the complex was assessed by 4-15% gradient SDS-PAGE using Mini-PROTEAN® TGX™ Precast Protein gels (BIORAD). Concentration of the RNAP in these fractions was determined by absorbance at 280 nm using NanoDrop™ One/OneC Microvolume UV-Vis Spectrophotometer (Thermofisher ...
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bioRxiv - Cancer Biology 2024Quote: ... Gel electrophoresis was performed on 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad Laboratories AG, Cat#4561094/4561096). Proteins were transferred to Trans-Blot Turbo 0.2 µm Nitrocellulose Transfer Membrane (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... Transfer of the proteins from gel to the Immun-Blot® Low Fluorescence PVDF membrane with low fluorescent transfer buffer (BIORAD; #1704275) was performed in Trans-Blot Turbo Transfer System (BIORAD ...
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bioRxiv - Biophysics 2024Quote: ... The pellet was then resuspended in binding buffer (50mM Tris, 120 mM NaCl, pH 7.4) Bradford protein assay (Bio-Rad, Hercules, CA) was used to determine the protein concentration and the membranes were diluted to 1000 μg/mL and stored in a −80 °C freezer for later use ...
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bioRxiv - Cell Biology 2024Quote: ... The protein concentrations of cell lysates were measured using BCA assay (Thermo 23225) and equal amounts of proteins were loaded onto 4 – 15 % polyacrylamide gradient gels (Bio-Rad 4561084) and fractionated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Neuroscience 2024Quote: ... Equal quantities of proteins from each individual sample were separated on mini-Protean® TGX™ mini pre-cast gels (BioRad, #4561023) at 120V until the leading dye reached the bottom of the gel ...
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bioRxiv - Genetics 2024Quote: ... The pulled down proteins were separated by Sodium dodecyl sulfate Poly-Acrylamide Gel Electrophoresis (SDS-PAGE) and transferred to polyvinylidene difluoride membranes (Bio-Rad Laboratories). Membranes were incubated with anti-ACTB (Proteintech ...
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bioRxiv - Neuroscience 2024Quote: ... Equal amounts of proteins (30µg) were separated by 7.5% SDS-PAGE and transferred to nitrocellulose membranes (Bio-Rad, Marnes-la-Coquette, France). After blocking membranes in Tris Buffer Solution with Tween-20 0.1 M (TBST ...
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bioRxiv - Developmental Biology 2023Quote: ... Protein lysates were resolved by SDS/polyacrylamide gel electrophoresis (PAGE) and transferred to polyvinylidene fluoride (PVDF) membranes (Bio-Rad, Hercules, CA, USA). After blocking ...
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bioRxiv - Developmental Biology 2024Quote: ... Protein samples were boiled for 10 minutes at 95 °C and loaded onto Mini-PROTEAN TGX™ Precast Gels (BIO-RAD:4569036), and electrophoresis was performed according to manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2024Quote: 40 μg of protein lysate was resolved on NuPAGE Novex 4-12 % Bis-Tris gels and transferred onto nitrocellulose membranes (Bio-Rad system). Membranes were blocked with 5 % BSA in TBS-T (10 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2024Quote: ... SDS-PAGE was performed using 10 μg of NP-40 extract protein mixed with an equal volume of 2X Laemmli buffer (BioRad cat#: 1610737) supplemented with 5% 2-mercaptoethanol (without boiling) ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were separated by size by running 20 μg of the protein extract in 8% SDS-PAGE and transferred to Immuno-Blot PVDF Membrane (Bio-Rad, 1620174) using the Trans-Blot® SD Semi-Dry Transfer Cell (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were separated by adding 20 μg of protein lysate to 4-20% gradient Mini Protean TGX Stain Free Pre-Cast Gels (Bio-Rad Laboratories). Proteins were then transferred to a nitrocellulose membrane (Cytiva ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were transferred to a 0.45 µm nitrocellulose membrane for proteins larger than 35 kDa and to 0.1 µm nitrocellulose or 0.2 µm PVDF membranes for proteins smaller than 35 kDa for 30 min using Trans-Blot Turbo transfer system (Bio-Rad, 1704150) or for 2.5 h using wet transfer system (TG buffer ...
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bioRxiv - Genetics 2023Quote: ... and luciferase activities were normalized to total protein levels in the WCEs extracts determined using the Bradford assay kit (Bio-Rad Laboratories).
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bioRxiv - Immunology 2023Quote: ... a total of 10–20 µg protein was prepared and loaded onto 4 to 20% gradient Mini-PROTEAN TGX Gels (Bio-Rad, 4561093) and electrophoretically separated at 135 V ...
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bioRxiv - Neuroscience 2023Quote: ... Cell lysates were separated on SDS/PAGE and proteins on the gels were transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). The membranes were blocked with 5% skim milk diluted in TBST for 1 h at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... A normalized volume equivalent to 20 μg total protein in the predigested sample was resolved on a 4-12% Criterion XT Bis-Tris gel (Bio-Rad 3450124) at 100V for 2 hours ...
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bioRxiv - Immunology 2023Quote: ... A total of 30 µg of protein per well was loaded onto a 4-15% precast SDS-PAGE gel (Bio-Rad, 4561083). SDS-PAGE running conditions are as follows ...
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bioRxiv - Microbiology 2023Quote: ... Protein samples were fractionated by SDS-polyacrylamide gel electrophoresis using a 4%–20% Mini-PRO TEAN TGX Gel (BioRad, Hercules CA, USA), and the resolved proteins were transferred by electroblotting onto polyvinylidene difluoride membranes (Immobilon-P PVDF Transfer Membranes) ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were then vortexed for 1 min at max speed and loaded into a 10% sodium dodecyl sulfate (SDS)-polyacrylamide gel and run at constant 100 V for 1 h 45 min in running buffer (25 mM Tris, 190 mM Glycine, 3.46 mM SDS) alongside a Precision Plus Protein Dual Color Standard (Bio-Rad, Cat #1610374) in ...