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Citations for Bio-Rad :
9151 - 9200 of 10000+ citations for Cow Rho Associated Protein Kinase 1 ROCK1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2020Quote: ... 10 to 25 μg for experiments that were imaged digitally) was loaded onto a 4–15% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad) and run either at 50 V for 10 min followed by 100 V for at least 1 h ...
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bioRxiv - Microbiology 2020Quote: ... The proteins were transferred to a Polyvinylidene difluoride (PVDF) membrane using the Trans-Blot® Turbo™ Transfer System (Bio-Rad). Detection of GFP was carried out with a rabbit polyclonal anti-GFP antiserum (Bio-Rad ...
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bioRxiv - Developmental Biology 2021Quote: ... Blots were then washed 3x 10 min in TBS followed by imaging of Stain Free total protein signal with a ChemiDoc MP System (Bio-Rad). Following stain-free imaging ...
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bioRxiv - Molecular Biology 2021Quote: ... samples were subjected to SDS-polyacrylamide gel electrophoresis and proteins were blotted onto nitrocellulose membranes using the Mini Trans-Blot Cell system (Bio-Rad).
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bioRxiv - Neuroscience 2020Quote: ... Bovine Serum Albumin (BSA) with concentrations 0.125 to 2.0mg/mL from a standard set served as protein standards (BioRad, Cat. Number 5000207). Then ...
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bioRxiv - Synthetic Biology 2019Quote: ... The purity and size of the proteins were validated on SDS-PAGE gel (Mini-PROTEAN TGX and Mini-TETRA cell, Bio-Rad). Purified proteins were stored at –20° C ...
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bioRxiv - Genomics 2019Quote: All protein isolation steps were performed at 4 °C or on ice and protein concentrations were assessed with the Bradford assay (Bio-Rad). Protein from total pancreas lysates was isolated from a small fragment of mouse pancreas and minced in lysis buffer (20 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2020Quote: ... The lysates were loaded onto 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Cat# 4561086, Bio-Rad, Hercules, CA), and proteins were subsequently transferred onto a polyvinylidene fluoride (PVDF ...
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bioRxiv - Synthetic Biology 2021Quote: ... Total proteins were separated in SDS-PAGE and transferred to a PVDF membrane using Transblot turbo (Bio-Rad Laboratories, CA, USA). After blocking the membrane with 0.3% skim milk in TBS-T ...
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bioRxiv - Synthetic Biology 2021Quote: ... The purity and size of the proteins were validated on a SDS-PAGE gel (Mini-PROTEAN TGX and Mini-TETRA cell, Bio-Rad). Purified proteins were stored at –20° C.
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bioRxiv - Microbiology 2020Quote: ... Samples were checked for purity and presence of protein using 15% SDS-PAGE (Polyacrylamide gel electrophoresis, Mini Protean 3 System, Bio-Rad) and Coomassie Brilliant Blue (Merck ...
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bioRxiv - Microbiology 2020Quote: ... proteins were blotted onto nitrocellulose membranes (Whatman, Maidstone, United Kingdom) using a Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad) under conditions suggested by the manufacturer ...
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bioRxiv - Microbiology 2020Quote: ... Protein bands for both of the gels were transferred to polyvinylidene fluoride (PVDF) membranes (Immun-Blot® 0.2 μm, Bio-Rad) using semi-dry electroblotting system (Trans-Blot® TurboTM Transfer System ...
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bioRxiv - Microbiology 2020Quote: ... The protein concentration of the supernatant was determined by Bradford measurement according to the manufacturer’s instructions (Bio-Rad, cat n° 5000006).
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bioRxiv - Microbiology 2020Quote: ... The protein samples were separated by either 15% SDS-glycine-PAGE or SDS-tricine-PAGE including the Precision Plus Protein Dual Xtra molecular weight marker (Bio-Rad). To run samples under nonreducing conditions ...
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bioRxiv - Biophysics 2020Quote: ... 15 μg of proteins were loaded onto gels prior to conducting SDS-PAGE and transferring onto polyvinylidene difluoride membranes (Bio-Rad). Membranes were then incubated with monoclonal antibodies against Cyclin B1 (PC133 ...
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bioRxiv - Neuroscience 2019Quote: ... Dynabead-antibody complexes were washed 3x with 1 ml IP buffer and 3x with 1X PBS and proteins were eluted at 70°C for 10 min in 2X Laemmli sample buffer (50 μl; Bio-Rad) containing 5% 2-mercaptoethanol ...
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bioRxiv - Neuroscience 2020Quote: ... following the manufacturer’s instructions and 12 µg of protein were loaded into 4-15% gradient gels (Biorad Miniprotean TGX, Biorad, Munich, Germany) and transferred to nitrocellulose membranes ...
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bioRxiv - Microbiology 2021Quote: ... 5 µL of samples were loaded into a precast 4-20% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) gels (Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad) alongside size standards (Precision Protein Plus ...
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bioRxiv - Immunology 2021Quote: 50 ug of protein was prepared in Protein Sample Loading Buffer (Li-Cor Biosciences) and loaded on Mini-PROTEAN 4-20% TGX Stain-Free Precast Gels (Bio-Rad). Immunoblotting was performed as detailed in the Supplementary Materials and Methods ...
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bioRxiv - Immunology 2020Quote: ... Samples were boiled for 3 min and sonicated for 30 s prior to loading on 4-20% Mini-PROTEAN TGX precast protein gels (Bio-Rad) and electrophoresis in Tris-glycine buffer ...
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bioRxiv - Physiology 2019Quote: ... The total protein (about 50 μg) was separated in an SDS-PAGE (10 %) and transferred onto nitrocellulose membrane (Bio-Rad, America). The membranes were blocked with TBST (137 mM NaCl ...
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bioRxiv - Developmental Biology 2019Quote: ... were used to standardize amount of total protein loaded on a gel 12% polyacrylamide gel was then transferred to polyvenyidene fluoride (PVDF membrane, Bio-Rad). 5% nonfat dry milk in PBST (Phosphate buffered saline with Tween 20 ...
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bioRxiv - Genetics 2019Quote: ... Separated proteins were transferred to PVDF membrane at 350 mA constant for 90 minutes using the Mini-TransBlot system (Bio-Rad). Transfer was validated by ponceau stain (Sigma) ...
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bioRxiv - Genomics 2019Quote: The volume for approximately the same number of cells for each sample across the mitotic release time course was loaded in each lane of a 4-12% bis-tris protein gel (Biorad 3450125) and separated in 1X MES running buffer (Biorad 1610789) ...
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bioRxiv - Cell Biology 2021Quote: ... 5 μg of plasmid DNA corresponding to each protein of interest was thoroughly mixed with 500 μL of the resuspended cells in an electroporation cuvette (Bio-Rad). This cuvette was subject to an electroporation pulse (280 V ...
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bioRxiv - Cell Biology 2020Quote: Transfected cells were lysed in RIPA buffer on day 4 and proteins extracted were separated by 10% - 15% SDS polyacrylamide gel and transferred to Nitrocellulose membranes (Bio-Rad) at 100V for 1 hour and blocked for 1 hour with 5% non-fat milk (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... Fractions containing IADs and OAD were determined by SDS-PAGE (4-20% Mini-PROTEAN TGX precast protein gels run in SDS buffer (Bio-Rad)) ...
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bioRxiv - Physiology 2021Quote: ... and 30 μg of protein per sample was electrophoresed by SDS PAGE on 4–20% Mini-PROTEAN TGX Stain-Free Precast Gels (Bio-Rad) and transferred to polyvinylidene fluoride membranes (Millipore) ...
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bioRxiv - Biochemistry 2020Quote: ... Protein content was determined by BCA assay (Pierce) and proteins were separated by SDS-PAGE and transferred onto nitrocellulose membrane (Bio-Rad). Membranes were blocked ...
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bioRxiv - Cell Biology 2021Quote: ... Protein content was measured by Bradford assay and 30 mg of protein per sample were loaded into 4-20 % precast SDS-PAGE gels (Bio-Rad) under denaturing conditions ...
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bioRxiv - Physiology 2020Quote: ... Twelve micrograms of protein were subjected to SDS-PAGE on 4-20% Criterion TGX stain-free gels (Bio-Rad, Hercules, CA) and transferred to polyvinylidene diflouride membranes (PVDF ...
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bioRxiv - Developmental Biology 2021Quote: Proteins extracted from Padi4CTL and Padi4CKO were subjected to SDS–PAGE (4–20% Mini-PROTEAN® TGX™ Precast gel, Biorad) and then transferred onto a nitrocellulose membrane ...
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bioRxiv - Microbiology 2021Quote: ... the relative amount of each protein was calculated using Quantity One software (densitometer GS-800; Bio-Rad Laboratories, Hercules, CA, USA), and was presented as a percentage of total protein [25].
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bioRxiv - Bioengineering 2021Quote: ... proteins were transferred to a polyvinylidene fluoride (PVDF) membrane (Cytiva) using a Trans-Blot SD Semi-Dry Electrophoretic Cell (Bio-Rad) with 1.5 mA / cm2 membrane for 1 h ...
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bioRxiv - Biochemistry 2021Quote: Equal amount of protein was loaded and resolved on 10% polyacrylamide gel and transferred onto the PVDF membrane supplied by Bio-Rad, USA ...
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bioRxiv - Biochemistry 2021Quote: ... 80 μg of total protein for each sample was resolved by SDS-PAGE prior to transfer to nitrocellulose membranes (Bio-Rad). Membranes were blocked in Tris-buffered saline + 0.1% (v/v ...
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bioRxiv - Biochemistry 2021Quote: ... The recovery of tag-less MlaC was quantified using the ratio standard curve method (41) while that of His-tagged nanodisc-embedded complexes were quantified using the Lowry assay (DC Protein Assay, Bio-Rad). For retrograde assay ...
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bioRxiv - Neuroscience 2021Quote: ... The densities of the bands of interest were normalized to the relative expression of GAPDH or Total Protein staining by TGX stain-free pre-cast gels (4568096, Bio-Rad), SYPRO Ruby (S4942 ...
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bioRxiv - Biochemistry 2021Quote: ... Proteoliposomes or protein-free liposomes were generated by two rounds of treatment with 300 mg of wet SM-2 Bio-Beads (Bio-Rad) in total ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins (20 μg – 40 μg depending on the target protein) were resolved by 10%-12% SDS-PAGE and transferred to polyvinylidene fluoride (PVDF) membranes (BioRad, USA). The membranes were blocked with 5% non-fat milk for 2 h at RT and subsequently probed with specific primary antibodies overnight at 4°C (Table EV2) ...
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bioRxiv - Cancer Biology 2021Quote: ... the equal quantity of protein (40 µg) was resolved on 10 % polyacrylamide gel and transferred onto the PVDF membrane supplied by Bio-Rad, USA ...
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bioRxiv - Cancer Biology 2021Quote: ... MA) and 35 μg of protein were loaded and size-fractionated by SDS-polyacrylamide gel electrophoresis (Bio-Rad Laboratories, Hercules, CA). Gels were then transferred to PVDF membranes ...
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bioRxiv - Genetics 2021Quote: ... Equal amounts of proteins were resolved by polyacrylamide-SDS gel electrophoresis (PAGE) and transferred to nitrocellulose membrane (Bio-Rad, Hercules, CA) using Semi-Dry Transfer Cell (Bio-Rad) ...
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bioRxiv - Immunology 2022Quote: ... was centrifuged at 500 x g for 10 min Protein levels in the supernatant were measured by Bradford assay (Bio-Rad), and cytokine levels were measured using a custom multiplex ELISA (ThermoFisher Scientific).
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bioRxiv - Cancer Biology 2021Quote: ... The resolved proteins were transferred to Immuno-Blot® PVDF membranes using a Trans-Blot® Turbo™ Transfer System (BioRad). Membranes were incubated at 4°C overnight in primary antibodies against GRP78 ...
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bioRxiv - Pathology 2021Quote: ... and equal volumes of each suspension were loaded into two sodium dodecyl sulphate-polyacrylamide gels (SDS-PAGE; 4-20% Mini-PROTEAN TGX Protein Gels, Bio-Rad) and run at 150 V for one hour ...
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bioRxiv - Molecular Biology 2021Quote: ... Equal amounts of total protein were separated on SDS-PAGE gel by electrophoresis and transferred to a polyvinylidene difluoride (PVDF) membrane (Bio-Rad). Target proteins were detected using the following antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... Bolt 4-12 % Bis-Tris Plus Gels and Bolt MES SDS Running Buffer 20X were used for protein separation before transfer to nitrocellulose membranes (Transblot Turbo Transfer Pack, Bio-Rad) using a Transblot Turbo 0.2 μm (Bio-Rad) ...
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bioRxiv - Biochemistry 2021Quote: Protein samples were separated employing SDS-PAGE as described previously (Laemmli 1970) using a MiniProtean III electrophorese Unit (Bio-Rad, Germany) and stained with Coomassie Brilliant Blue (Thermo Scientific ...