Labshake search
Citations for Bio-Rad :
9051 - 9100 of 10000+ citations for Cow Rho Associated Protein Kinase 1 ROCK1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2021Quote: ... 10% of the bead volume was transferred to a new tube and were boiled in 30 μL of 2X protein loading buffer (Biorad, 1610737) supplemented with 2 mM biotin and 20 mM dithiothreitol (DTT ...
-
bioRxiv - Neuroscience 2021Quote: ... The cAMP levels were normalized to the total protein concentration in the supernatant determined by Bradford Assay using BSA standard curve (Bio-Rad). The final cAMP concentration is expressed as pmol cAMP per mg of total protein ...
-
bioRxiv - Neuroscience 2020Quote: ... Equal amounts of protein were subjected to westernblot analysis using precast polyacrylamide gels (10-20% Tris-HCl or 4-12% Bis-Tris, Bio-Rad). For immunoprecipitation (IP ...
-
bioRxiv - Cell Biology 2021Quote: ... samples were boiled for 5 min and proteins were resolved on 10% SDS polyacrylamide gels and blotted onto nitrocellulose membranes (Bio-Rad). Anti-rabbit Phospho-p44/42 MAPK antibody (Cell Signaling Technology ...
-
bioRxiv - Cancer Biology 2020Quote: ... 30 μg of protein was loaded into each lane of a TGX-stain-free pre-cast 4–20% SDS gel (Biorad, 4568094), total protein was quantified upon stain-free gel imaging ...
-
bioRxiv - Neuroscience 2021Quote: ... Incubation with appropriate HRP-conjugated secondary antibodies (Thermo) was for 2 hours at room temperature and protein bands were visualized using the Clarity Western ECL Substrate (BIO-RAD). Densitometric analysis was performed using ImageJ software (NIH).
-
bioRxiv - Cancer Biology 2020Quote: ... Equal amount of protein was resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using Criterion TGX Stain-Free Precast Gels (Bio-Rad) and transferred onto Immobilon-P membranes (MilliporeSigma) ...
-
bioRxiv - Neuroscience 2020Quote: ... Equal amount of proteins from each lysate were subjected to polyacrylamide gel (4-10 %) electrophoresis (PAGE) in Mini-PROTEAN Tetra cell apparatus (Bio-Rad). PAGE separated proteins were blotted onto a PVDF membrane in a semi-dry Trans-Blot Turbo transfer system (Bio-Rad) ...
-
bioRxiv - Physiology 2020Quote: ... Twelve micrograms of protein were subjected to SDS-PAGE on 4-20% Criterion TGX stain-free gels (Bio-Rad, Hercules, CA) and transferred to polyvinylidene diflouride membranes (PVDF ...
-
bioRxiv - Biochemistry 2021Quote: ... and 5 mM β-mercaptoethanol) until the washes indicate a protein concentration of less than 0.1 mg/mL by Bradford assay (Bio-rad). The protein was eluted with elution buffer (25 mM Tris pH 8.0 ...
-
bioRxiv - Biochemistry 2020Quote: ... The denatured samples were loaded onto AnyKD™ Mini-PROTEAN® TGX™ precast protein gels (Bio-Rad, Hercules, CA, USA) and separated for 40 min at 40 mA ...
-
bioRxiv - Bioengineering 2021Quote: ... 15 µg of the extracted protein samples were boiled for 5 minutes at 95 °C in 4X laemlli buffer (BioRad; 1610747), run on mini-PROTEAN TGX precast gels (Bio-Rad ...
-
bioRxiv - Synthetic Biology 2021Quote: ... The proteins were characterized by electrophoresis using a 12% SDS polyacrylamide gel and imaged using a ChemiDoc Touch Imaging System (Bio-Rad).
-
bioRxiv - Neuroscience 2020Quote: ... and 25-100 µg of total protein was loaded onto 8 to 15 % SDS-PAGE minigels (Bio-Rad Laboratories, Hercules, CA) prior transfer onto PVDF membranes ...
-
bioRxiv - Microbiology 2020Quote: ... 7.5 μg per lane of total protein samples were separated on 4-12% Bolt Bis-Tris Plus Gels and blotted onto polyvinylidene difluoride membrane (Bio-Rad) using the Trans-Blot Turbo Transfer System (Bio-Rad) ...
-
bioRxiv - Zoology 2021Quote: ... These standardized data were then normalized to total protein from the same membranes using a SYPRO Ruby Blot Stain (Bio-Rad). The averages of phosphorylated MAPK/total MAPK/total protein in the hearts from treatment fish ...
-
bioRxiv - Microbiology 2021Quote: ... Proteins were eluted in the denaturing mode and run in a 4-20% Mini-Protean TGX stain-free precast protein gel (Bio-Rad) at 200V for 30 min and stained by Imperial protein stain (ThermoFisher ...
-
bioRxiv - Microbiology 2021Quote: ... The soluble fraction was subjected to affinity chromatography at 4°C composed of 75 μL of amylose resin bound to 200 pmol of MS2-MBP protein in a Bio- Spin disposable chromatography columns (Bio-Rad). After washing ...
-
bioRxiv - Genetics 2021Quote: ... The 13 μl of protein was run on a 10% SDS-PAGE gel and transferred to a PDVF membrane by semidry transfer (Bio-Rad) at 13V for 90 min ...
-
bioRxiv - Microbiology 2021Quote: The eluted IP samples were separated by molecular weight using SDS-PAGE (precast 4-20% Mini-PROTEAN TGX Stain-Free Protein Gels, 10 well, 50 μl; Bio-Rad) at 160 V for 10 min ...
-
bioRxiv - Physiology 2021Quote: ... Protein aliquots were denatured in Laemmli sample buffer at 100°C for 5 min and then loaded (10– 15 μg protein per lane) on 4–20% Criterion TGX Stain-free gels (Bio-Rad) and run for 45 min at 200 V ...
-
bioRxiv - Microbiology 2021Quote: Protein samples were resolved by SDS-PAGE using 4-12% Bis-Tris NuPAGE precast gels with MES running buffer alongside the Precision Plus prestained protein standard (Bio-Rad). Proteins were transferred to nitrocellulose membranes following SDS-PAGE using an iBlot 2 Dry Blotting System (Thermo) ...
-
bioRxiv - Microbiology 2020Quote: ... The proteins of interest were further purified using a Superdex 200 pg column on an NGC liquid chromatography system (Bio-Rad). Each fraction was resolved on Novex 4–20% tris–glycine gels with Coomassie blue staining to assess protein purity.
-
bioRxiv - Microbiology 2020Quote: Surface Plasmon Resonance (SPR) binding experiments were carried out on a ProteOn XPR36 6×6 channels protein interaction array system (Bio-Rad). Proteins BRD4-BD1 an BRD4-BD2 were diluted to a final concentration of 50μg/ml in HEPES buffer 25 mM pH 7.0 and immobilized to a GLM chip using amine coupling ...
-
bioRxiv - Cancer Biology 2020Quote: ... 30–40 µg of total protein were loading into each well of a 10% SDS-PAGE gel and separated using an electroporation system (Bio-Rad). Proteins were transferred onto an NC membrane (Whatman) ...
-
bioRxiv - Cancer Biology 2020Quote: ... Equal amounts of protein were separated via SDS-PAGE and transferred to a PVDF membrane using Trans-Blot Turbo System (Bio-rad). The following antibodies were used for detection ...
-
bioRxiv - Cancer Biology 2021Quote: ... 10 μg of proteins were then separated on a 4-15% Mini-PROTEAN TGX Stain-Free Gel (Bio-Rad, Ref: 4568085) at 160V ...
-
bioRxiv - Cancer Biology 2021Quote: ... Fifty micrograms of protein from each sample was loaded and separated in 7.5% SDS-PAGE gel (4561024, Bio-Rad, Hercules, CA) and transferred onto a 0.2 mm PVDF membrane using the wet transfer method (90 V for 90 min) ...
-
bioRxiv - Cancer Biology 2020Quote: ... Between 50 and 100 µg of total protein was loaded onto a gradient (4-15%) polyacrylamide gel (BioRad, Hercules, CA, USA). Proteins were then transferred to a polyvinylidene difluoride (PVDF ...
-
bioRxiv - Cell Biology 2021Quote: ... Equal amounts of protein were then separated by SDS-PAGE on 4-20% polyacrylamide gels (Bio-Rad Laboratories, Richmond, VA, USA) at 200V for 40min ...
-
bioRxiv - Neuroscience 2020Quote: ... Equivalent total protein amounts from BH and EVs were separated on 4−15% stain-free pre-cast SDS-PAGE gradient gels (Bio-Rad) under non-reducing conditions and transferred onto PVDF membranes (Sigma Aldrich) ...
-
bioRxiv - Microbiology 2021Quote: ... The mCherry-fusion lysate (input) and proteins eluted from the beads were separated on a 4–20% TGX SDS-PAGE gel (Bio-Rad) and transferred to a PVDF membrane for immunoblot analysis ...
-
bioRxiv - Cell Biology 2020Quote: ... The pellet was resuspended in 10 µl 4M Urea and the protein concentration of both pellet and supernatant was determined by using the method of Bradford (5000006, Bio-Rad). Finally ...
-
bioRxiv - Cell Biology 2020Quote: Proteins were separated on a polyacrylamide gel with 10% of sodium dodecyl sulfate(SDS) (Mini Protean III; Bio-Rad, Richmond, CA), and they were transferred for 2 hours at 48°C to nitrocellulose membranes of 0.45 μm ...
-
bioRxiv - Cell Biology 2020Quote: ... Triton-insoluble material was then pelleted at 15890 x g for 10 min and protein concentration measured with the DC assay (Bio-rad). For immunoblot analysis ...
-
bioRxiv - Cell Biology 2021Quote: ... 100 µg of protein were loaded per lane and electrophoresed on a 10% TGX precast SDS PAGE gel from Bio-Rad. Protein was transferred onto a nitrocellulose membrane ...
-
bioRxiv - Cell Biology 2022Quote: ... were then activated with 100% methanol and total protein extracts were transferred for 14 min at 25 V using a Trans-Blot Turbo Transfer System (Bio-Rad). Membrane-bound proteins were visualized with Ponceau S (78376 ...
-
bioRxiv - Microbiology 2022Quote: ... Equivalent amounts of protein from cellular lysates or fractions were electrophoresed on pre-casted gels (Bio-Rad Laboratories, Hercules, CA, USA) or in-house prepared 15% polyacrylamide gels and electrophoretically transferred to nitrocellulose membranes using the Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad Laboratories) ...
-
bioRxiv - Microbiology 2022Quote: ... and growth factor protein expression was quantified using the Bio-Plex Pro Rat Cytokine 23-plex assay (Bio-Rad Laboratories, Inc). Analytes included in the assay were ...
-
bioRxiv - Molecular Biology 2022Quote: ... the supernatant was transferred into a new tube and and the protein concentration was determined using a Bradford assay (Biorad #5000006) as described by the manufacturer ...
-
bioRxiv - Biophysics 2022Quote: ... The labelled proteins were further purified and separated from free Alexa Fluor dye using a Superdex 75 10/300 GL gel filtration column (Cytiva) using either an ÄKTA protein purification system (Cytiva) or NGC Chromatography System (Bio-Rad). The proteins were concentrated using the Amicon Ultra centrifugal filters (Merck ...
-
bioRxiv - Physiology 2022Quote: ... Supernatants were transferred to a new tube and an aliquot were used for protein quantification using the Bradford assay (Bio-Rad). A total of 10-20 μg of protein lysates were separated on 8% gels by SDS-PAGE and transferred to nitrocellulose membranes ...
-
bioRxiv - Plant Biology 2022Quote: ... The extract was centrifuged at 18,000 g for 10 minutes at 4 °C and the protein content of the supernatant determined using the Bradford method (Bio-Rad) in a spectrophotometer measuring the absorbance at 595 nm and then eluted in Laemmli 2X buffer with 8 % β-mercaptoethanol (95 °C for 5 minutes) ...
-
bioRxiv - Biochemistry 2022Quote: ... The samples were sonicated and then resolved by SDS-PAGE on 10% gels along with Precision Plus All Blue protein prestained standards (Bio-Rad). After SDS-PAGE ...
-
bioRxiv - Molecular Biology 2022Quote: ... the proteins were transferred to nitrocellulose membranes in a Turbo blotter (Trans-Blot Turbo Blotting System, 170-4155, Bio-Rad Laboratories). The total protein amount was visualized using the stain-free image of the blot (Figure 1B) ...
-
bioRxiv - Neuroscience 2022Quote: ... A total of 10 µg protein was then separated by SDS/PAGE and transferred to Immuno-Blot PVDF membranes (Bio-Rad). Membranes were incubated in 5% non-fat milk for 1 hour at room temperature to block nonspecific binding ...
-
bioRxiv - Genetics 2022Quote: ... The 13 µl of protein was run on a 10% SDS-PAGE gel and transferred to a PDVF membrane by semidry transfer (Bio-Rad) at 13V for 2 hours 15 min ...
-
bioRxiv - Cancer Biology 2022Quote: ... The Cy7.5 signal corresponding to persulfidated proteins was recorded at 800nm and the NBF-adducts signal was recorded at 488nm on a Chemidoc Imager (BioRad, France). Persulfidation levels were assessed by normalizing the Cy7.5 intensity to Alexa488 (NBF-adducts ...
-
bioRxiv - Neuroscience 2022Quote: ... was performed for 2 h at room temperature and protein bands were visualized using the Clarity Western ECL Substrate (BIO-RAD).
-
bioRxiv - Bioengineering 2022Quote: ... run at 120 V constant voltage (Pharmacia LKB, ECPS 3000/150) using a Hoefer gel system and Precision Plus Protein Kaleidoscope standards (Bio-Rad). Protein and peptide concentrations were determined by direct measurement on a Nanodrop 1000 UV-Vis spectrophotometer (Thermo Scientific) ...