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Citations for Bio-Rad :
9401 - 9450 of 10000+ citations for Cow Rho Associated Protein Kinase 1 ROCK1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Pushed to the edge: hundreds of myosin 10s pack into filopodia and could cause traffic jams on actinbioRxiv - Cell Biology 2024Quote: ... and heated at 70°C for 10 min before loading onto a 4-20% Mini-PROTEAN TGX Stain-Free protein gel (BioRad, 4568095). The gel was run at 180 V for 45 min and imaged on a ChemiDoc ...
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bioRxiv - Biochemistry 2024Quote: ... Fractions containing protein were pooled and desalted using the Econo-Pac® 10-DG Desalting Columns (Bio-Rad, Cat#732-2010). To this end ...
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bioRxiv - Bioengineering 2024Quote: ... Gels were stained for proteins using Coomassie R-250 and subsequent gel images were captured on a Chemidoc MP Imaging System (Bio-Rad).
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bioRxiv - Molecular Biology 2024Quote: ... and 0.1 vol of DTT 1M was added and the mixture heated at 95°C for 5 min before loading onto Any kD Mini-PROTEAN TGX Stain-Free protein gel (4568124, Bio-Rad) and migration in 1x Tris-Glycine-SDS buffer at 200V ...
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bioRxiv - Bioengineering 2024Quote: ... SDS-PAGE was performed using 4–15% Mini-PROTEAN® TGX™ Precast Protein Gel (#4561084, Bio-Rad Laboratories, Inc, USA). Before and after the laser exposure ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were boiled for 5 min and 20 to 30 μg of protein were separated by SDS–polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes (Bio-Rad). Membranes were blocked in 3% milk in Tris-buffered saline with 0.1% Tween 20 (TBST ...
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bioRxiv - Molecular Biology 2024Quote: ... as well as either Coomassie Blue staining (for the TGX Precast Protein Gel) or the ChemiDoc Touch Imaging System (Bio-Rad) (for the TGX Stain-Free™ Protein Gel).
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bioRxiv - Neuroscience 2024Quote: ... Samples were split and electrotransferred using 4-20% Mini-PROTEAN TGX Stain-Free Protein Gels and the PVDF membrane Trans-Blot Turbo Transfer System (Bio-Rad Laboratories ...
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bioRxiv - Cancer Biology 2024Quote: ... ten micrograms of proteins were separated by SDS-PAGE and transferred onto 0.2 μm PVDF membranes (Bio-Rad Laboratories, Inc., 1704156) using the Trans-Blot Turbo Transfer System (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were eluted from beads using 400 mM Imidazole and their concentrations were determined using a Bradford Assay (BioRad, Hercules, CA) with BSA standard ...
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bioRxiv - Plant Biology 2024Quote: ... Stain-free gels were activated and total protein on gel were detected and recorded using a ChemiDoc™ Imaging System (BioRad).
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bioRxiv - Plant Biology 2024Quote: ... Twenty-five μg of protein per well were analyzed in an SDS-PAGE using a Mini-PROTEAN Tetra electrophoresis system (Bio-Rad) and transferred to a Trans-Blot transfer nitrocellulose membrane (Bio-Rad) ...
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bioRxiv - Neuroscience 2024Quote: ... Approximately 15 μg protein was separated by SDS-PAGE on a precast 4-12% Criterion XT Bis-Tris gel (Bio-Rad) and transferred to a nitrocellulose membrane using TurboBlot (Bio-Rad) ...
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bioRxiv - Neuroscience 2024Quote: ... Each gel was transferred to a methanol-activated Immobilon-FL PVDF membrane (MilliporeSigma) which was then UV-activated and imaged for total protein content on a Chemidoc MP (Bio-Rad). Membranes were blocked at room temperature for 1 hr in a 1:1 mixture of PBS and fluorescent blocking buffer (Rockland Immunochemicals ...
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bioRxiv - Bioengineering 2024Quote: The outcome of the reaction was analysed using native PAGE (4-20% Mini- PROTEAN TGX Precast Protein Gels, Bio-Rad, 4561096). 1 µL of the retic lysate reaction was diluted with 2 µL 1×PBS and mixed with 2× sample buffer containing 62.5 mM Tris ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were centrifuged at 15700 g for 30 min at 4°C and protein concentration was determined by Bradford assay (Bio-Rad). 600 µM of GppNHp Rab8a1-176 was titrated into the cell containing 30 µM bMERB domain and for full-length Mical1 ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were separated by SDS-PAGE on 16% acrylamide gels immobilized by transfer to a PVDF membrane (Cat. #1620177, Bio-Rad) using a Trans-Blot Turbo Transfer System (Trans-Blot ®TurboTM ...
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bioRxiv - Microbiology 2024Quote: ... Samples were denatured for 10 min at 95°C and then 10 µl were loaded onto a precast 4-15% protein gel (Bio-Rad). Electrophoresis was performed by applying a constant voltage of 200 V in SDS running buffer (Tris/Glycine/SDS ...
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bioRxiv - Microbiology 2024Quote: ... Fractions with the protein of interest were concentrated and further subjected to anion exchange chromatography on a Resource Q (Bio-Rad) 6 ml-column to remove remaining contaminants with a NaCl salt gradient from 50 mM to 500 mM ...
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bioRxiv - Cell Biology 2024Quote: ... A total 20 μg protein per extract was boiled in 1X reducing sample buffer and used for SDS-PAGE (BioRad, 4561083). Validation of gene knockdowns was performed by Western blotting using primary antibodies against STARD7 (Proteintech ...
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bioRxiv - Molecular Biology 2024Quote: ... for at least 30 min at 4 °C cleared by centrifugation and protein concentration was determined by Bradford assay (Bio-Rad).
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bioRxiv - Molecular Biology 2024Quote: ... the cells were irradiated with 400 mJ/cm2 UV light to fixate the RNA-protein complexes by covalent cross-linking in the cells (GS Gene Linker UV Chamber, Bio-Rad); except for the control samples which were not UV-treated ...
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bioRxiv - Molecular Biology 2024Quote: ... boiled for 10 min at 95°C and examined by western blot using 4-15% Mini-PROTEAN TGX precast protein gels (BioRad #4561083). After blocking step with 10% non-fat milk for 1h at room temperature ...
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bioRxiv - Immunology 2024Quote: ... Proteins lysates were denatured with DTT and boiled prior to separation by SDS-PAGE and transferred onto PVDF membranes (Bio-Rad). Primary antibody incubations (Supplementary Table 2 ...
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bioRxiv - Neuroscience 2024Quote: ... The protein chemiluminescence signal was obtained and visualized with the ChemiDocTM Touch Gel Imaging System (Bio-Rad Laboratories, Hercules, CA, USA). After FasII or NCAM1 detection ...
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bioRxiv - Microbiology 2024Quote: ... protein concentrations were measured using the BCA assay and 20 μg of proteins from whole cell lysates were resolved on pre-cast 12% tris-glycine PAGE (Bio-Rad) followed by transfer to a LF-PVDF membrane using a Bio-Rad semi dry transfer apparatus ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were immediately lysed in ice cold Radioimmunoprecipitation Assay (RIPA) buffer and protein was quantified using a Bradford Assay (Bio-Rad), per manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... Complementary DNA was synthesized using the reverse transcription kit (PrimeScript RT reagent Kit) and quantitative (q)PCR was performed using iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories, Hercules, CA). The housekeeping gene GAPDH was used for reference ...
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bioRxiv - Microbiology 2021Quote: ... 1:5000; # HAF017, R&D Systems, goat anti-rabbit, 1:5000; #170-6515, Bio Rad, goat anti-mouse, 1:5000; #6516, Bio-Rad). To ensure that equal amounts of proteins were loaded ...
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bioRxiv - Immunology 2023Quote: ... Primary antibody staining was performed overnight at 4&C using rabbit anti-mouse Iba-1 (1:500; Wako Chemical Cat #019-19741) and CD68 (1:200; Bio-Rad Cat #MCA1957) with secondary staining performed for 45 minutes RT using Alexa Flour 594 donkey anti-rabbit (1:400 ...
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bioRxiv - Microbiology 2020Quote: ... Whole cell lysate was mixed 1:1 with 2x Laemmli solution (Bio-rad, 1610737) supplemented with B-mercaptoethanol (55 mM stock ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were developed with Clarity Western ECL Substrate 1:1 (Bio-Rad Laboratories, USA) and chemiluminescence signal was detected with a Chemidoc Touch camera (Bio-Rad Laboratories ...
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bioRxiv - Genetics 2021Quote: ... or 1:100 F4/80 rat anti mouse antibody clone A3-1 (Bio-Rad Laboratories ...
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bioRxiv - Developmental Biology 2019Quote: ... Supernatants were mixed at 1:1 (vol/vol) with 2X Laemmli Sample Buffer (Biorad) with 0.1M dithiothreitol (DTT ...
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bioRxiv - Neuroscience 2020Quote: ... Each sample was mixed 1:1 (10 μl + 10 μl) with Laemmli buffer (BioRad) and boiled at 95°C for 5 min before loaded onto a 4-12% SDS/PAGE gel and run at 200 V before electrotransferred to a membrane using Transblot-Turbo Transfer system (BioRad) ...
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bioRxiv - Neuroscience 2021Quote: ... after incubation for 1 hr with HRP-conjugated second antibodies (1:5000, Bio-Rad). Between each step ...
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bioRxiv - Cancer Biology 2020Quote: ... and incubated with secondary antibodies (mouse, Bio-Rad, 1:2500; rabbit, BioRad, 1:2500). Western HRP substrate kit was used to detect chemiluminescent signal (Millipore ...
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bioRxiv - Cancer Biology 2020Quote: ... and incubated with secondary antibodies (mouse, Bio-Rad, 1:2500; rabbit, BioRad, 1:2500). Western HRP substrate kit was used to detect chemiluminescent signal (Millipore ...
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bioRxiv - Genetics 2020Quote: ... anti-PGK (1:20,000) (Novex) with goat anti-mouse HRP (1:40,000) (Bio-Rad), or anit-H2B (1:80,000 ...
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bioRxiv - Genetics 2020Quote: ... anti-PGK (1:20,000) (Novex) with goat anti-mouse HRP (1:40,000) (Bio-Rad) or by anti-Smc3 K112/K113 Acetylation (1:1,000 ...
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bioRxiv - Molecular Biology 2022Quote: ... incubated 1 h at room temperature with 1/3000 anti-Mouse StarBright blue700 (BioRad) or 1/2000True blot HRP anti-rabbit antibodies ...
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bioRxiv - Genomics 2022Quote: ... anti-rabbit (1:5000; #7074, Cell Signaling Technology or 1:10000, #12004162, Bio-Rad), actin (1:5000 ...
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bioRxiv - Neuroscience 2023Quote: ... after incubation for 1 hr with HRP-conjugated second antibodies (1:5000, Bio-Rad).
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bioRxiv - Microbiology 2023Quote: ... Whole cell lysate was mixed 1:1 with 2x Laemmli solution (Bio-rad, 1610737) supplemented with B-mercaptoethanol (55 mM stock ...
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bioRxiv - Plant Biology 2020Quote: ... was reverse transcribed (iScript™ cDNA Synthesis Kit; Bio-Rad) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... reverse transcribed into cDNA (BIORAD iScript™ cDNA Synthesis Kit) and primers (indicated as followed ...
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bioRxiv - Cell Biology 2020Quote: ... followed by cDNA preparation (iScript cDNA synthesis kit; Bio-Rad). All oligonucleotide primers (see Supplemental Table 1 ...
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bioRxiv - Cell Biology 2020Quote: ... and reverse transcribed using the iScript cDNA synthesis kit (Biorad). Fixed amount of spike RNA (β-globin ...
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bioRxiv - Cell Biology 2020Quote: ... CDNA was synthesized using the iScript cDNA synthesis kit (Biorad) and made using an Eppendorf 5331 MasterCycler Gradient Thermal Cycler (Eppendorf ...
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bioRxiv - Cell Biology 2020Quote: ... followed by cDNA synthesis with iScript cDNA Synthesis Kit (BioRad). SYBR Green PCR Master Mix (Applied Biosystems ...