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Citations for Bio-Rad :
8951 - 9000 of 10000+ citations for Cow Rho Associated Protein Kinase 1 ROCK1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... either on 7–12% Tris-glycine gels or gradient NuPAGE 4–12% Bis-Tris Protein Gels (Thermo Fischer) or gradient Criterion TGX gel (4–15%) (BioRad). A list of antibodies and their working dilution used in this work are reported in (Table S3) ...
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bioRxiv - Bioengineering 2024Quote: ... protein from gel was transferred to a PVDF membrane using the Trans-Blot Turbo Transfer System (Bio-Rad, cat.: 1704150).
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bioRxiv - Cancer Biology 2023Quote: ... 25 μg protein extracts were separated by sodium dodecyl sulfate (SDS) gel electrophoresis and transferred to a polyvinylidene difluoride (PVDF) membrane (BioRad) overnight ...
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bioRxiv - Cell Biology 2023Quote: ... Protein extracts were mixed with Laemmli sample buffer and separated by SDS-PAGE using 4-20% TGX gels (Bio-Rad) and transferred to 0.2 µm nitrocellulose membranes (Bio-Rad ...
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A synthetic elastic protein as molecular prosthetic candidate to strengthen vascular wall elasticitybioRxiv - Cell Biology 2023Quote: ... Proteins were transferred onto poly(vinylidene fluoride) membrane using Trans-Blot Turbo Transfer System (Bio-Rad, Marnes-la-Coquette, France). After transfer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Total protein (mg/mL) was determined for each sample using Bradford assay reagent (Catalogue # 5000002, Bio-Rad, Hercules, California, USA). Liver mitochondria (0.175 mg/mL ...
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bioRxiv - Bioengineering 2023Quote: ... Protein transfer was performed on methanol-activated polyvinylidene fluoride (PVDF) membranes by Trans-Blot Turbo Transfer system (1704150; Bio-Rad). After transfer the membranes were blocked in 5% BSA in TBS with 0.1% Tween-20 for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... Western blots were loaded at 2.5 to 50 ug total protein per lane in 4-20% gradient pre-cast polyacrylamide gels (#3450033, Bio-Rad). Gels were also loaded with the Dual pre-stained protein standards (#1610374 ...
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bioRxiv - Immunology 2024Quote: ... Cell lysates were separated by SDS-PAGE 4 - 12 % gradient Bis-tris gel (Novex) followed by protein transfer to a PVDF membrane (BioRad) and antibody incubation ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were transferred from gels to the PVDF membrane using the Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad). Membranes were incubated in 5% nonfat dried milk in 1X TBST (Tris-buffered saline ...
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bioRxiv - Cell Biology 2024Quote: ... 10 µg of protein was boiled for 5 min at 95°C and then loaded on a 4-15% SDS gel (Biorad).
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bioRxiv - Cell Biology 2024Quote: ... and the probed proteins were transferred to Bio-Rad polyvinylidene fluoride membranes using a semidry electroblotting transfer system (Bio-Rad) at 25 V for 2 h and 30 min at room temperature ...
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bioRxiv - Immunology 2024Quote: ... 70µg of protein was loaded per well for polyacrylamide gel electrophoresis using Mini-PROTEAN Precast Polyacrylamide Gels (Bio-Rad, 4561095). Gel was transferred to a low fluorescence PVDF membrane (Cytiva ...
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bioRxiv - Neuroscience 2024Quote: ... and 10 µg of protein was separated on a 4–20% sodium dodecyl sulfate polyacrylamide gel electrophoresis (Bio-Rad, #4561096) and blotted onto nitrocellulose membranes by wet transfer ...
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bioRxiv - Bioengineering 2024Quote: All relative protein quantities from western blotting were calculated by performing densitometry using ImageJ or Image Lab software (Bio-Rad) and normalizing to the average of the replicates of the PrgIWT samples ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were loaded onto 10% bis-Tris gels and transferred to Immun-Blot PVDF membranes (1620177, Bio-Rad, Hercules, CA), which were then blocked in 5% blocking grade skim milk (170–6404 ...
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bioRxiv - Developmental Biology 2024Quote: ... of total protein in Laemmli Buffer were subjected to electrophoresis on precast polyacrylamide gels and transferred to PVDF membranes (BioRad). Membranes were blocked and probed overnight with primary antibodies ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were transferred to a nitrocellulose membrane with a Trans-Blot®Turbo™ Transfer Starter System (BioRad, 170-4155). For high molecular weight samples (SYNJ1-BirA* ...
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bioRxiv - Cell Biology 2024Quote: Twenty μg of protein was loaded in each well of a Mini-protean TGX gel (Bio-Rad 4561083; Hercules, CA) with Precision Plus Protein Dual Color Standards (Bio-Rad 1610374 ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were loaded onto either a 4–20% Mini-PROTEAN® TGX Stain-Free™ Protein Gel (4568094, Bio-Rad) or 4– 20% Mini-PROTEAN® TGX™ Precast Protein Gel (4561094 ...
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bioRxiv - Neuroscience 2024Quote: ... Bis-Tris, 1.0 mm, Mini Protein Gel, 10-well (ThemoFisher, NP0321BOX) and transferred to polyvinylidene fluoride (PVDF) membranes (Biorad, 1704156EDU). PVDF membranes were blocked with 5% nonfat dried milk in Tris-buffered saline (TBS ...
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bioRxiv - Cell Biology 2024Quote: ... protein bands (IP or TCE) were transferred from polyacrylamide gel to a PVDF membrane using a TransBlot Turbo device (BioRad). PVDF membrane was blocked for 10 min with 8% blocking milk in Tris-buffered saline ...
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bioRxiv - Neuroscience 2024Quote: ... The resulting supernatant was transferred to a clean tube and protein concentration was determined using a Bradford assay (Bio-Rad). 200 μg tissue homogenate or 10 μg cell lysate was diluted in 50 μl CB lysis buffer and was used for the reaction with CatB Substrate (RR-amino-4-trifluoromethyl coumarin (AFC)).
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bioRxiv - Neuroscience 2024Quote: ... 80-100 μg of protein were loaded onto 4–20% Mini-PROTEAN® TGX™ Precast Gel (Biorad, 4561093EDU, USA) and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were denatured in Laemmli loading buffer as previously described,30 25 µg of protein was separated by 7.5% SDS-polyacrylamide acrylamide gel electrophoresis (SDS-PAGE) (Criterion, Bio-Rad) and blotted onto nitrocellulose membranes ...
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bioRxiv - Neuroscience 2024Quote: ... and the separated proteins were transferred onto PVDF membranes using a Trans-Blot Turbo semi-dry transfer system (Bio-Rad). Membranes were blocked using 3% BSA in TBS with 0.1% Tween20 (TBST) ...
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bioRxiv - Biochemistry 2024Quote: ... lysates were then spun down at 14,000 rpm for 10 mn before determining protein concentration using Bradford reagent (Bio-Rad).
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bioRxiv - Neuroscience 2024Quote: ... Proteins were separated by gel electrophoresis and transferred to nitrocellulose membranes using a semi-dry blotting system (Bio-Rad Laboratories). Membranes were blocked with 3% milk or 5 % BSA in TBST for 1 h at RT ...
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bioRxiv - Biochemistry 2024Quote: ... Cholesterol values were calculated using known cholesterol solutions and normalized to protein content as measured by DC assay (Bio-Rad).
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bioRxiv - Biochemistry 2024Quote: Polar flagellins purified from bacterial growths of each mutant strain (2 µg) were separated by 12% SDS-PAGE as described previously [31] and stained with Coomassie Blue total protein stain (BioRad), according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2024Quote: ... Thermo #26616) were run in pre-cast 4– 20% TGX stain-free denaturing protein gels (Biorad #4568093, #4568096, #5678094, #5678095) in a minigel system or criterion system (Biorad #1658005 ...
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bioRxiv - Cell Biology 2024Quote: ... Equivalent micrograms of protein from each sample were diluted using 4X Laemmli buffer (Bio-Rad, Hercules, CA, USA, Cat # 1610747) supplemented with β-mercaptoethanol (Millipore-Sigma ...
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bioRxiv - Immunology 2024Quote: ... proteins were transferred onto a nitrocellulose membrane using the Mini Trans-Blot® Cell and Criterion™ Blotter (Bio-Rad). Proteins on the membrane were stained with Ponceau S buffer ...
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bioRxiv - Cell Biology 2024Quote: ... Protein signals were detected with Super ECL Detection Reagent (Yeasen, 36208ES60) in a ChemiDoc™ MP Imaging System (Bio-Rad). Native proteins were extracted using Column Tissue & Cell Protein Extraction Kit (Epizyme ...
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bioRxiv - Synthetic Biology 2024Quote: ... Protein purity and size were validated on an SDS-PAGE gel (Bio-Rad Mini-PROTEAN TGX and Mini-TETRA cell). Purified proteins were stored at –80°C.
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bioRxiv - Microbiology 2024Quote: ... HKU1 NTD and pA-LS NP protein expression was analyzed using SDS-PAGE and subsequent Western blot on PVDF membrane (Biorad) using StrepMAB-Classic HRP 1:3000 (2-1509-001 ...
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bioRxiv - Microbiology 2024Quote: ... Samples were denatured in Laemli Buffer and boiled for 10 minutes then run on a 4-20% Criterion TGX precast midi protein gel (BioRad) and transferred to a PVDF membrane (Millipore Sigma) ...
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bioRxiv - Immunology 2024Quote: ... 7.5 µg of proteins were separated on polyacrylamide gel and then transferred on nitrocellulose membrane by the Trans-Blot dry system (Biorad). Membranes were blocked on milk (5% ...
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bioRxiv - Molecular Biology 2024Quote: ... Tissue lysates containing equal amounts of total protein were mixed with 4x Laemmli Sample Buffer (Bio-Rad, Hercules, CA, USA) and incubated at 96 °C for 5 min ...
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bioRxiv - Molecular Biology 2024Quote: ... Cell extracts were cleared at 5000g for 5min at 4°C and protein concentration was quantified by the Bradford assay (BioRad). Finally ...
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bioRxiv - Microbiology 2024Quote: ... sonicated and their protein contents were analysed by electrophoresis on TGX Stain-free pre-cast SDS-polyacrylamide gel (Bio-Rad). Proteins were then transferred on PVDF membranes (GE Healthcare ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were transferred from gels to nitrocellulose membranes for Western Blot analysis using the Turbo-Blot transfer system (Bio-Rad). Membranes were blocked using 1% w/v BLOTTO (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2020Quote: ... 25 μL of lysate mixed with sample buffer (1:1 ratio) (Bio-Rad) was loaded per lane in a 10% SDS-PAGE gel (Bio-Rad) ...
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bioRxiv - Physiology 2020Quote: ... and rat anti-F4/80 (1:500, Clone A3-1, MCA497GA, Biorad, USA). After 3×5 min washes ...
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Incidence of an intracellular multiplication niche amongst Acinetobacter baumannii clinical isolatesbioRxiv - Microbiology 2021Quote: ... Phalloïdine 647N ATTO (1/200) and DAPI nuclear dye (Bio-rad,1/1000) were diluted in the blocking solution ...
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bioRxiv - Microbiology 2022Quote: ... and mixed 1:1 with 2x Laemmli Buffer (Bio-Rad Laboratories, 161-0737). 10 μg of protein was loaded from each sample into separate wells of a 4-15% polyacrylamide gel (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2023Quote: ... the samples were mixed 1:1 with 2X Laemmli sample buffer (Bio-Rad) with 5% β-mercaptoethanol (Sigma ...
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bioRxiv - Immunology 2023Quote: ... and rabbit anti-alpha actinin-1 at 1:1000 (Bio-Rad Laboratories #VPA00889). Membranes were washed with TBS-T and then incubated with HRP-conjugated secondary antibodies for 1 h at RT ...
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bioRxiv - Developmental Biology 2020Quote: ... Upon removal of genomic DNA (TURBO DNA-free™ Kit or RNase-free DNase Set) cDNA was synthesized using the iScript™ cDNA Synthesis Kit (Bio-Rad). PCR reactions were run in triplicate with 6.25-12.5 ng cDNA per reaction using the CFX96 Real-Time PCR Detection System (BioRad) ...
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bioRxiv - Plant Biology 2020Quote: ... Protein concentration of the cytosolic fraction from each sample was measured using the Bradford assay (Protein Assay Dye Reagent Concentrate; Bio-Rad). After normalization ...