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Citations for Bio-Rad :
8901 - 8950 of 10000+ citations for Monkey Caveolin 1 CAV1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... 40 mg of total protein was loaded onto a 12-15% polyacrylamide gradient gel (Mini-PROTEAN TGX Precast Mini Gel; Bio-Rad Laboratories) and transferred to a nitrocellulose membrane following separation ...
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bioRxiv - Cell Biology 2022Quote: ... To verify the generation of single chain antibodies we prepared samples of whole and reduced antibodies with NuPAGE LDS Sample Buffer (4x) and run them in NuPAGE™ 7% Tris-Acetate Protein Gels (Bio-Rad) in denaturing ...
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bioRxiv - Neuroscience 2019Quote: ... Tissue blocks were sectioned by freezing microtome at 50 μm intervals and immunohistochemical staining was performed for protein gene product 9.5 (PGP 9.5; rabbit polyclonal; AbD Serotec, a Bio-Rad Company, Kidlington, UK) using a free-floating protocol ...
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bioRxiv - Pathology 2019Quote: ... Affi-gel 10 affinity resins were covalently cross-linked to his-tagged GT198 protein as antigen for affinity purification of anti-GT198 according to the manufacturer’s protocol (Bio-Rad, Hercules, CA). FFPE tumor sections or tumor microarrays were deparaffinized and dehydrated through xylene and ethanol series ...
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bioRxiv - Biophysics 2019Quote: ... Fractions were analyzed after separation in stain-free precast SDS-PAGE gels (4-20% Mini-PROTEIN® TGX Stain-Free™, Biorad). These stain-free gels contain trihalo compounds that undergo UV-induced covalent modification of tryptophan residues for subsequent fluorescent detection ...
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bioRxiv - Biophysics 2019Quote: ... The purified protein samples obtained after the final SEC step were analyzed by stain-free precast SDS-PAGE gels (4-20% Mini-PROTEIN® TGX Stain-Free™, Biorad).
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bioRxiv - Biophysics 2019Quote: ... was utilized to monitor the elution of the proteins and fractions were analyzed after separation on 4– 15% precast gradient TGX Stain-FreeTM gels (Bio-Rad Laboratories).
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bioRxiv - Physiology 2019Quote: ... The immunoreactive protein bands were revealed by ECL prime Western blotting detection reagent (Ammersham™) and detected using ChemiDoc™ Imaging System (Biorad). Densitometry quantification was performed using image lab software (v5.2.1 ...
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bioRxiv - Developmental Biology 2019Quote: ... and 1X phosphatase inhibitor cocktail and equal amounts of protein samples were loaded and run in a 4 % to 20 % SDS gel (Bio-Rad Laboratories). Protein was transferred to a polyvinylidene fluoride membrane (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2019Quote: ... Lysates were collected and equal protein amounts were separated by SDS-PAGE and blotted onto a nitrocellulose membrane by wet-blotting (Bio-Rad, Germany). Membranes were blocked with 5% milk or 5% BSA in TBS containing 0.1% Tween 20 (TBS-T ...
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bioRxiv - Microbiology 2019Quote: The purity of the proteins was checked using SDS-PAGE electrophoresis in a vertical electrophoretic mini-cell unit (Bio-Rad, Hercules, CA), in Tris-glycine running buffer (25mM Tris ...
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bioRxiv - Plant Biology 2019Quote: ... The mixed beads were washed once with the washing buffer and biotinylated proteins were eluted by 30 μL 2× SDS sample buffer and separated by SDS-PAGE (Biorad, Hercules, CA).
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bioRxiv - Cancer Biology 2020Quote: 10 μg of protein samples harvested from xenografted human cells were separated in pre-casted 4-15% gradient SDS gels (Biorad, #456-1084) and transferred to nitrocellulose membranes (Millipore #IPVH00010) ...
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bioRxiv - Microbiology 2020Quote: ... 20 μL of normalized lysates were loaded into pre-cast 4-20% SDS-PAGE gels (4-20% Mini-PROTEAN® TGX™ Precast Protein Gels, BioRad) alongside size standards (Precision Protein Plus™ ...
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bioRxiv - Developmental Biology 2020Quote: ... and separated protein was transferred from gels to polyvinylidene fluoride membranes (PVDF) using a Trans-Blot Turbo Transfer system (Bio-Rad Laboratories). The membrane was blocked with 5% Bovine Serum Albumin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... Total 20 to 25 μg of protein samples were resolved by sodium dodecyl sulfate polyacrylamide electrophoresis (SDS-PAGE; Bio-Rad, Madrid, Spain) followed by Western blot ...
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bioRxiv - Pathology 2021Quote: ... CST #2118) in 5% bovine serum albumin (total protein antibodies) or 5% dry milk (phospho-specific antibodies) Tris buffered saline (Bio-Rad #1706435) with 0.1% Tween-20 ...
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bioRxiv - Plant Biology 2020Quote: ... Incubate 1 μL of reticulocyte extract before binding with beads and 2 μL of extract after the binding was used for each western on a precast gel (4–20% Criterion™ TGX™ Precast Midi Protein Gel, 12+2 well, 45 μl, BioRad). Before loading ...
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bioRxiv - Cancer Biology 2021Quote: Protein (20 µg) was separated electrophoretically on SDS polyacrylamide gels and then transferred to a nitrocellulose membrane (BIO-RAD Laboratories, Mississauga, ON). After blocking ...
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bioRxiv - Cancer Biology 2020Quote: ... The protein samples were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (PAGE) and transferred to a nitrocellulose membrane (Bio-Rad, Canada). The expression levels of ABCB1 ...
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bioRxiv - Microbiology 2020Quote: ... Forty μg of total proteins were separated by electrophoresis on 10% polyacrylamide gels and transferred to PVDF membranes (Bio-Rad, CA, USA). The membranes were blocked with 5% BSA in Tris-buffered saline for 1 h at room temperature ...
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bioRxiv - Microbiology 2021Quote: Cell lysates were run under reducing conditions on an Any kD™ Mini-PROTEAN® TGX Stain-Free™ Protein Gel (BioRad) and imaged using a ChemiDoc imaging system (Biorad) ...
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bioRxiv - Biochemistry 2020Quote: ... Purification of the desalted bacteriocin protein was performed using an Uno S-6 prepacked monolith cation exchange column (12×55 mm, Bio-Rad, USA) on a FPLC system (BioLogic DuoFlow System ...
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bioRxiv - Neuroscience 2019Quote: Protein extracts were loaded at 20μg per lane and resolved by standard electrophoresis in 4-20% Mini-PROTEAN TGX Precast polyacrylamide protein gels (Bio-Rad, Hercules, CA). Gels were transferred onto polyvinyl difluoride membranes (PVDF ...
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Checkpoint adaptation in repair-deficient cells drives aneuploidy and resistance to genotoxic agentsbioRxiv - Cell Biology 2019Quote: ... using 7.5 % polyacrylamide gels for Rad53 detection or a gradient of 4-15 % polyacrylamide for all other proteins in Mini-PROTEAN® electrophoresis chambers (Bio-Rad). Proteins were transferred to 0.45 µm pore size nitrocellulose membranes (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... the desalted protein fraction was purified using the Uno S6 prepacked monolith cation exchange column (12 × 53 mm, 6 mL, Bio-Rad, USA). The eluted fraction containing purified bacteriocin was concentrated to 4.6 mg/mL in an Amicon centrifugal filter concentrator with a 3 kDa cutoff membrane (Millipore ...
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bioRxiv - Bioengineering 2020Quote: Mouse tissues from mdx4cv mice treated with or without AAV9-NG or AAV9-iNG were lysed with cold RIPA buffer supplemented with protease inhibitors and extracted protein samples were separated by SDS-PAGE (BioRad, 4-15%) and transferred onto Nitrocelluloase membranes (0.45 μm) ...
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bioRxiv - Pathology 2019Quote: ... 2019) Thirty micrograms of total proteins were separated by SDS-PAGE gel (Mini-Protean Precast Acrylamide Gels, Bio-Rad, Hercules, CA, USA) and further transferred to the nitrocellulose membrane (Trans-Blot Turbo transfer pack ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were detected by specific primary antibody and secondary antibody then visualized using the ChemiDoc MP Imaging System (Bio-Rad, Hercules, CA).
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bioRxiv - Immunology 2021Quote: ... 10 μg of total cell lysates proteins were separated by electrophoresis on 10% SDS polyacrylamide gels (previously prepared following the Bio-Rad protocol) and transferred to a polyvinylidene difluoride (PVDF ...
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bioRxiv - Genetics 2021Quote: ... Equal amounts of proteins (40 μg) were loaded onto 10% Tris-glycine gel (CriterionTM XT precast gel) using XT Tricine Running Buffer (Bio-Rad, USA). After electrophoresis ...
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bioRxiv - Cell Biology 2020Quote: ... and 8 μg of protein was added from each sample to a 26-well Criterion TGX 4-20% precast gel (Bio-rad, 5671095). Proteins were transferred to an Immun-Blot low flouresence PVDF membrane (Bio-rad ...
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bioRxiv - Physiology 2021Quote: ... the membranes were rinsed three times in TBS-T for 5 min and the proteins were visualized by enhanced chemiluminescense (BioRad, Clarity 1705061) in a BioRad Universal III Hood with Image Lab software v.6.0.1 ...
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bioRxiv - Biochemistry 2021Quote: Intensities of bands corresponding to RBD proteins were measured using Gel Doc 2000 and Image Lab software (Bio-Rad, Hercules, CA, USA) in order to measure protein expression levels ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... and 20 µg of protein from each sample was separated by SDS-PAGE on 4–20% precast polyacrylamide gel (Bio-Rad, 4561096). Western blot analyses were performed according to standard protocols using the following antibodies ...
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bioRxiv - Plant Biology 2021Quote: ... 20 μl of eluted protein samples were separated on SDS gel (12%) and transferred to PVDF membrane (Immun-Blot® PVDF, Bio-Rad) through semi-wet blotting protocol ...
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bioRxiv - Cell Biology 2021Quote: ... 1∼2 μg of total protein for each lane) was loaded on a precast 4-20% gradient polyacrylamide gel (PAGE, Bio-Rad: 4561096) and electrophoresed at a constant voltage of 200 V for 35 min ...
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bioRxiv - Cancer Biology 2021Quote: ... and 20 μg of proteins per sample were resolved in 10 or 12% gels (TGX™ FastCast™ Acrylamide kit, Bio-Rad) via electrophoresis ...
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bioRxiv - Developmental Biology 2021Quote: ... Proteins were separated by SDS-PGE on a 4-15% Mini-PROTEAN® TGX Stain-Free™ gel (Bio-Rad #456-8084) and transferred with the Trans-Blot® Turbo™ RTA Transfer Kit (Bio-Rad #170-4275 ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were centrifuged at 20,000 x g for 10 min at 4°C and protein concentration in the supernatant was determined by Bradford assay (Bio-Rad, 5000202) and normalized across samples ...
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bioRxiv - Cell Biology 2021Quote: ... and the protein concentration was determined by interpolation on to absorbance curves generated by recombinant protein standards using iMark™ Microplate Absorbance Reader (Bio-Rad).
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bioRxiv - Evolutionary Biology 2020Quote: ... 1-5 μg of purified antigen or 20 μg protein extract were loaded in a precast 4-20% acrylamide gel (BioRad 456-1094). Gels were run for the first 20-30 min at 40V ...
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bioRxiv - Immunology 2020Quote: ... the supernatant was discarded and the Protein G beads were resuspended in sterile PBS and transferred into Spin columns (Micro Bio-Spin Chromatography Columns, Bio-Rad, UK). The beads were washed 4 x with sterile PBS before addition of 117 µl Glycine pH 2.7 per sample and incubated for 5 min at room temperature ...
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bioRxiv - Immunology 2020Quote: ... 1ug of purified recombinant S RBD-His6 was diluted in PBS and Laemmli buffer to a final volume of 20 μl and added to a 7.5% Mini-PROTEAN® TGX™ Precast Protein Gel (BIO-RAD). Resolved proteins were then transferred to a PVDF membrane ...
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bioRxiv - Microbiology 2020Quote: ... or lacking (non-reducing) 100 mM β-mercaptoethanol and resolved by SDS-PAGE in a 4-20% Mini-PROTEAN TGX Precast Protein Gel (BIO-RAD). Proteins were transferred to nitrocellulose membranes using a Trans-Blot SD Semi-Dry Transfer Cell (BIO-RAD ...
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bioRxiv - Molecular Biology 2021Quote: ... Equal volumes of leaf protein were separated by SDS-PAGE (Mini-PROTEAN® TGX stain-free™ precast gels; Bio-Rad system) and cys-OLE was detected by immunoblotting as described in Winichayakul et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... even protein amounts were loaded and separated on mini-PROTEAN TGX precast 4-15% Bis-Tris gels (#4568085, Bio-Rad, Copenhagen, Denmark). Using a Bio- Rad Trans-Blot semidry transfer system proteins were transferred to a nitrocellulose membrane (#1704159 ...
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bioRxiv - Immunology 2020Quote: ... equal protein amounts of the lysates (25 μg) were applied to the Bio-Plex Mouse Cytokine 23-plex Assay Kit (Bio-Rad Laboratories) and measured with the BioPlex200 system and BioPlex-Manager 6.2 according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and then proteins in the gel were transferred to PVDF membrane using Trans-Blot Turbo Mini PVDF Transfer Packs (Bio-Rad, USA) and Trans-Blot Turbo System (Bio-Rad) ...
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bioRxiv - Cell Biology 2021Quote: ... GFP-Cdc42p and ubiquitin were detected under non-saturated conditions and normalized to the housekeeping protein Pgk1p using the Image Lab Software (Bio-Rad, Inc.). Wild-type cells and control conditions were set to 1 and adjusted for other samples accordingly.