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Citations for Bio-Rad :
8901 - 8950 of 10000+ citations for Cow Rho Associated Protein Kinase 1 ROCK1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were diluted 1:10 in RIPA Lysis and Extraction buffer (Thermo Fischer) supplemented with protease inhibitor cocktail (Fischer Scientific) for use in BCA protein assay (BioRad). BCA protein assay was performed in triplicate for each diluted sample following manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Radio-labeled RNA-protein complexes were extracted from beads by boiling in 70 μl of SDS loading buffer (Bio-Rad), separated on a 4-12% Bis-Tris gel ...
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bioRxiv - Molecular Biology 2023Quote: ... Obtained samples were denatured in a 1xLaemmli buffer at 95°C for 10 min and proteins separated using electrophoresis on a 4-15% mini-protean-TGX-precast-gel (BioRad). Proteins were then transferred on nitrocellulose membranes by a semi-dry method using transblot turbo transfer system (BioRad) ...
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bioRxiv - Pathology 2023Quote: ... Equal quantities of protein (30 - 60 μg) were resolved on TGX-precast gels (Bio-Rad Laboratories Inc., Hercules, CA, USA) and subsequently transferred to PVDF blotting membranes (Millipore ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were then separated on 10-20% SDS-polyacrylamide gels and were visualized by staining with Coomassie blue (Bio-Rad).
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bioRxiv - Microbiology 2023Quote: ... gels were briefly washed with water and proteins were transferred (25 V, 1.3 A, 7 min) onto nitrocellulose membranes (Bio-Rad) using Bio-Rad Trans Blot Turbo Transfer system ...
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bioRxiv - Microbiology 2023Quote: ... Cells were lysed and protein reduced by mixing 25μl of sample to 4.75 μl Laemmelli buffer (Bio-Rad #161-0737) and 0.25ul of beta-mercaptoethanol (Bio-Rad #161-0710 ...
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bioRxiv - Immunology 2023Quote: ... 15 to 30 µg of protein sample was processed for separation through SDS PAGE on 4–20% Criterion TGX precast gradient gels (BioRad). Gel run consisted of 15 min run at 70V followed by 45 min run at 100V ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of protein (5 µg) were loaded into each lane and separated on 4– 20% polyacrylamide Tris glycine SDS gels (BioRad), then transferred to 0.45 µm PVDF membranes (Millipore Sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... The lysates were pelleted at 10,000 g for 10 min and the supernatants were used to determine the protein concentration using Bradford assay (Bio-Rad). After a preclearing step with Protein G Sepharose (Sigma) ...
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bioRxiv - Biochemistry 2022Quote: ... Separated protein bands were visualized using “Stain Free Gel” application mode on ChemiDoc MP Imaging System (Bio-Rad Laboratories, Inc.). Protein gel was transferred to a nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were resolved by SDS/PAGE and transferred to nitrocellulose membranes using the Trans-Blot Turbo transfer system (Bio-Rad). Membranes were blocked in 5% BSA in Tris-buffered saline containing Tween 20 (TBS-T ...
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bioRxiv - Cell Biology 2022Quote: ... 10-50 μg protein was loaded on 10% acrylamide home-made or gradient (4-15% acrylamide) commercial (BIO-RAD, 4561086) Tris-Glycine-SDS gels along a protein ladder (PageRuler prestained protein ladder ...
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bioRxiv - Cancer Biology 2023Quote: ... 30-50 µg of nuclear proteins were loaded onto a 10% SDS-PAGE and transferred on nitrocellulose membrane using TransBlot apparatus (BioRad). Membranes were subsequently incubated with TBS Tween-20® 0.01% ...
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bioRxiv - Cancer Biology 2023Quote: ... SDS-PAGE resolved proteins were transferred onto a nitrocellulose membrane using the Trans-Blot Turbo™ transfer system (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Purified GST-KIN-EC-ter fusion protein from pooled gel filtration fractions was cross-linked to Affi-gel 15 beads (BioRad) in MOPS ...
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bioRxiv - Cell Biology 2023Quote: ... 20 μg of protein per sample was resolved on a NuPage 4-12% Bis-Tris polyacrylamide mini gel (Bio-Rad) and transferred to a nitrocellulose membrane in Towbin transfer buffer containing 20% MeOH at 100V for 1 hour or 25V for 16 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates (35 µg protein) were run on 10% SDS-polyacrylamide gels and transferred to a nitrocellulose membranes (Bio-Rad, 1620115). After blocking for 10 min with 5% nonfat dried milk in PBS with 0.05% Tween-20 (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2023Quote: ... Membranes were washed and blocked in 1x TBST with 5% bovine serum albumin before consecutive incubation with primary antibody (see Suppl. Table 3) and horseradish peroxidase-coupled protein A (Cymed) and detection using Clarity Western ECL Substrate (BioRad).
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bioRxiv - Immunology 2023Quote: ... Bead-bound proteins were then eluted in 2x Laemmli buffer (diluted from 4x Laemmli Sample Buffer, #161-0747, Bio-Rad) with betamercaptoethanol (#21985023 ...
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bioRxiv - Immunology 2023Quote: ... eluted proteins were run on 10% polyacrylamide gels and transferred to polyvinylidene fluoride (PVDF) membranes (Trans-Blot Turbo Transfer System, BioRad). Membranes were blocked with 5% milk in Tris-buffered saline (TBS ...
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bioRxiv - Physiology 2023Quote: ... Proteins were denatured in Laemmli buffer and separated on 10% SDS-PAGE gels and transferred to PVDF membranes (Bio-Rad). The membranes were blocked with blocking buffer for 1 h in TBST containing 5% BSA or 5% milk ...
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bioRxiv - Biochemistry 2023Quote: ... The concentrated protein was run on a Superdex Increase 200 (Cytivia) gel-filtration FPLC column on Biologic DuoFlow System (Biorad). The fraction corresponding to the peak corresponding to the molecular mass of the monomer protein (∼80kDa ...
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bioRxiv - Microbiology 2023Quote: ... SDS-PAGE was performed in 1X MES buffer and proteins were transferred onto 0.2 µm nitrocellulose membranes (Bio-Rad, 1620112).
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bioRxiv - Cell Biology 2023Quote: ... Purity was confirmed by loading 1.125 µg of protein on a 12-well 12% Mini-Protean TGX gel (Bio-Rad), performing SDS-PAGE electrophoresis and fixing the protein with 40% methanol ...
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bioRxiv - Cell Biology 2023Quote: ... or poly-acrylamide gels and proteins were transferred overnight at 4°C on a nitrocellulose membrane using a liquid transfer system (BioRad). Non-specific sites were blocked with 5% non-fat dry milk in PBS 0.1% Tween 20 ...
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bioRxiv - Physiology 2023Quote: ... and left ventricle of wild-type C57 Bl6 mice was incubated with PC2 antibody (10 ug, Alomone laboratories) overnight complexed to magnetic protein G beads (Biorad). Following 3 rounds of washing ...
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bioRxiv - Immunology 2023Quote: ... between 20-30 mg protein was run on 10-20% SDS–PAGE gradient gels and transferred onto 0.22 μM PVDF membranes (1620177, Bio-Rad). After air drying to return to a hydrophobic state ...
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bioRxiv - Genetics 2023Quote: ... Quantitative analysis of protein levels was carried out using a GS-800 calibrated densitometer with ImageLab software (Bio-Rad Laboratories).
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bioRxiv - Cell Biology 2023Quote: ... and 10 μg protein was loaded per lane on Mini-PROTEAN TGX Precast Gels (Bio-Rad Laboratories, Hercules, CA, USA). Following protein separation ...
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bioRxiv - Cell Biology 2023Quote: ... The appropriate HRP-conjugated secondary antibodies were used for the chemiluminescent detection of proteins with Clarity Max Western ECL Substrate (BioRad). Membranes were scanned with a ChemiDoc imaging system (BioRad ...
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bioRxiv - Biochemistry 2023Quote: ... the proteins on the gel were transferred to a polyvinylidene fluoride membrane using a Transblot SD semi-dry transfer system (BioRad) at 25V for 30 min ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were resolved by SDS/PAGE and transferred to nitrocellulose membranes using the Trans-Blot Turbo transfer system (Bio-Rad). Membranes were blocked with 5% BSA in Tris-buffered saline containing Tween 20 (TBS-T ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were denatured and proteins were separated by SDS-PAGE in Criterion™ TGX Stain-Free™ Gels (Bio-Rad), and transferred onto nitrocellulose membranes with the Trans-Blot® Turbo™ Blotting System (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: ... The samples adjusted to contain equal amounts of protein (10 μg or 12 μg) were next denatured (5 min at 95°C) in loading buffer (#1610747, BioRad) and run on 10% TGX (#4561034 ...
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bioRxiv - Biochemistry 2024Quote: ... the absorbance of standards and protein samples was measured at 490 nm using a microplate absorbance reader (Bio-Rad iMarkTM). See Fig ...
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bioRxiv - Physiology 2024Quote: ... Equivalent amounts of total protein were separated by SDS-PAGE on 4% to 15% Criterion TGX gels (Bio-Rad; 5671083). Megalin and cubilin were detected with anti-megalin antibody generously provided by Dr ...
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bioRxiv - Physiology 2024Quote: ... Protein extracts were denatured at 95 °C for 10 min and separated in 10% SDS-PAGE gels (1610156, Bio-Rad) at 80 V for ∼45 min followed by 110 V for ∼65 min ...
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bioRxiv - Molecular Biology 2023Quote: ... 30 μg of total protein lysate was loaded for each sample onto Mini-PROTEAN® Precast Gels (Bio-Rad, 4568123) with 4 × Laemmli buffer (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were separated by SDS-PAGE electrophoresis and transferred to PVDF membranes using Trans-blot turbo transfer system (Bio-Rad). Membranes were incubated in 5% BSA in tris-buffered saline (TBS)-Tween 0.1% (TBST ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were separated by SDS-PAGE (30-50 μg total protein per lane) and transferred onto a 0.2 μm nitrocellulose membrane (Bio-Rad). Membranes were blocked with 5% skimmed milk for 30 min at RT and treated with primary antibodies (Supplementary Table S1 ...
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bioRxiv - Biophysics 2023Quote: ... Protein-DNA complexes were transferred from the gel to the PVDF membrane (Immun-Blot® PVDF Membrane, BIO-RAD, USA) using semi-dry blotting in 1x transfer buffer (48 mM Tris ...
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bioRxiv - Biophysics 2023Quote: ... protein signals were visualized by incubation of the membrane in ECL substrate (Clarity™ Western ECL Substrate, BIO-RAD, USA), and chemiluminescence was detected with Odyssey® Fc Imaging System (Li-COR ...
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bioRxiv - Biophysics 2023Quote: A total of 250 ng of purified proteins as labelled above each lane were loaded on TGX stain-free gels (BioRad) and run at constant 150V ...
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bioRxiv - Bioengineering 2023Quote: ... Protein transfer was performed on methanol-activated polyvinylidene fluoride (PVDF) membranes by Trans-Blot Turbo Transfer system (1704150; Bio-Rad). Post transfer the membranes were blocked in 5% Bovine Serum Albumin (BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... The column was washed with 50 mL of wash buffer and then transferred onto a Fast protein liquid chromatography system (BioRad). The column was further washed until the UV trace was flat ...
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bioRxiv - Cell Biology 2023Quote: ... Detection of proteins was achieved following 5 min incubation with Enhanced Chemiluminescence (ECL) reagent and exposed using a ChemiDoc imaging system (BioRad). Loading control was performed by normalizing the target protein to the stain-free images of total protein ...
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bioRxiv - Microbiology 2023Quote: ... proteins were resolved by SDS-PAGE and transferred onto a PVDF membrane by using a transfer apparatus (Bio-Rad, USA). The membrane was probed with primary antibody against V2c (1:4000) ...
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bioRxiv - Cell Biology 2023Quote: ... and equal amounts of protein were loaded and run on 4-20% gradient SDS-PAGE gels (4561096; Bio-Rad Laboratories). Proteins were transferred from SDS-PAGE gels to nitrocellulose membranes using Trans-Blot Turbo (Bio-Rad Laboratories) ...
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bioRxiv - Microbiology 2023Quote: ... followed by immunoprecipitation with 5 µg appropriate antibodies or corresponding IgG control using magnetic protein A/G beads (BIO-RAD). After several rounds of washing with low-salt wash buffer (20 mM Tris-HCl ...