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Citations for Bio-Rad :
9151 - 9200 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... RNA copy numbers were determined by ddPCR (see AAV virus quantification) using Taqman primers for the WPRE element (Sup. Table 1) and Rpp30 (Biorad, assay ID: dMmuCPE5097025) as housekeeping gene.
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bioRxiv - Neuroscience 2023Quote: The reference gene used in combination with the designed human PMP22 assay was the commercially established AP3B1 (dHsaCP1000001; #10031245, Bio-Rad, Basel, Switzerland) ddPCR copy number variation assay.
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bioRxiv - Microbiology 2023Quote: ... for the selected clinical isolates using the indicated concentrations of antibiotics via an alamar blue reduction assay completed as described but without shaking.8 After 3 days of incubation with the alamar blue reagent (BioRad, Hercules, CA, USA), the MIC was identified as the lowest concentration of drug that inhibited the reagent color change as determined visually.
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bioRxiv - Molecular Biology 2024Quote: ... the reverse primer and the probe sequences were designed using Primer3 following Bio-Rad guidelines for the primer/probe assay design (BioRad Bulletin 6407, n.d.). The designed primers and probe (Table 1 ...
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bioRxiv - Immunology 2024Quote: Circulating plasma levels of cytokines and chemokines in PTB patients were measured using the Luminex MAGPIX Multiplex Assay system (Bio-Rad, Hercules, CA). Luminex Human Magnetic Assay Kit 45 Plex (R&D Systems ...
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bioRxiv - Biochemistry 2024Quote: ... The lysates were cleared by centrifugation at 10,000 g for 5 minutes and protein concentration was determined using the DC Protein Assay (Bio-Rad Laboratories, Watford, UK) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Supernatant was transferred to a clean tube and total protein concentration was measured with the DC protein assay II (Bio-Rad #500-0112). Samples were stored at -80 until used.
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bioRxiv - Systems Biology 2024Quote: ... qPCR was performed in 20uL reaction volumes with KBTBD2 assay together with GAPDH and amplified using iTaq universal probes supermix (Bio-Rad Laboratories, #1725134). Relative quantification was performed using (2–ΔΔCt).
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bioRxiv - Immunology 2024Quote: Cytokine concentrations were measured in plasma samples using three panels (pro Human Cytokine, pro Human Chemokine, pro Human Inflammation) of the Bio-Plex Multiplex Assay (Bio-Rad Laboratories, USA) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: Cytokines and chemokines in BAL fluid were measured using the multiplex bead array system Bio-Plex Pro Mouse Chemokine Assay Panel 31-Plex (#12009159, Bio-Rad Laboratories GmbH), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... 30ug of protein as determined by Bradford protein quantification assay was loaded onto a mini-PROTEAN TGX stain-free gel (Bio-Rad, cat#4568084) and run at 200V for 25 minutes in SDS running buffer (.2501M Tris base (Fisher ...
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bioRxiv - Microbiology 2022Quote: ... and 1’,3’-bis[1-palmitoyl-2-oleoyl-sn-glycero-3-phospho]-glycerol (16:0-18:1 Cardiolipin) were spotted using a Hamilton syringe onto a nitrocellulose membrane (Biorad trans-blot turbo RTA Midi 0.2 µm) to yield 10 ...
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bioRxiv - Cell Biology 2021Quote: ... Resulting protein extracts were subjected to 10 % (w/v) SDS-polyacrylamide gel electrophoresis and transferred to PVDF membranes (Bio-Rad, Hercules, CA, USA, 0.2 μm pore size). Upon incubation with 5 % (w/v ...
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bioRxiv - Molecular Biology 2022Quote: ... Total protein extracts were separated by electrophoresis on 10% SDS-polyacrylamide gel and transferred to a TransBlot® Turbo Mini Size polyvinylidene difluoride (PVDF) membrane using the TransBlot® Turbo system (BioRad Laboratories, Inc., Hercules, CA, USA). The membranes were blocked with low-fat milk and probed with primary antibody ...
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bioRxiv - Cancer Biology 2024Quote: ... SDS-PAGE and transfer were performed on 4–15%Bio-Rad Mini/Midi PROTEAN®TGX™ and Trans-Blot®Turbo™ mini/midi nitrocellulose membranes using the Trans-Blot®Turbo™ Transfer System (Bio-Rad). Nonspecific binding sites were blocked using 5% non-fat dried milk in TBS-0.1% Tween-20 (TBS-T ...
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bioRxiv - Immunology 2021Quote: Total RNA was extracted using the PicoPure RNA Isolation kit and cDNA synthesis was performed using the iScript cDNA synthesis kit (Bio-Rad). cDNA samples were amplified with SYBR Select mastermix (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... First-strand cDNA for PCR analyses was made with a commercially available kit (iScript™ cDNA Synthesis Kit, Bio-Rad Laboratories). The quantification of mRNA expression was performed using the SYBR Green dye (Bio-Rad SsoAdvanced™ - Universal SYBR® Green Supermix ...
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bioRxiv - Microbiology 2020Quote: Total RNA was isolated either from homogenized mosquitoes or infected cell supernatant using a Multisource RNA Miniprep Kit (AP-MN-MS-RNA-250, Axygen) and reverse transcribed to cDNAs using an iScript cDNA Synthesis Kit (1708890, Bio-Rad). Viral genomes were quantified by qPCR using iTaq Universal SYBR Green Supermix (1725121 ...
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bioRxiv - Microbiology 2021Quote: ... using the primer-probe mix from the 2019-nCoV RUO kit (IDT) with iTaq Universal Probes One-Step kit (Bio-Rad). The 2019-nCoV_N_Positive Control plasmid (IDT ...
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bioRxiv - Cell Biology 2020Quote: ... and cDNA was reversely transcribed with 0.5–1 μg RNA using the iScript cDNA synthesis kit as described in the kit (Biorad, Hercules, CA). The gene expression of Il-6 ...
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bioRxiv - Cell Biology 2021Quote: RNA was extracted using the RNeasy plus micro RNA extraction kit followed by reverse transcription using iScript cDNA synthesis Kit (Bio-Rad). Equal amounts of DNA and corresponding primers (Supplementary Table 1 ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was extracted using the NucleoSpin RNA Plus Kit (Machery-Nagel) followed by reverse-transcription with the iScript cDNA synthesis kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Complementary DNA (cDNA) was synthesized using a Qscript kit (QuantaBio, MA) as per kit instructions in a T-100 (Bio-Rad) thermocycler.
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bioRxiv - Evolutionary Biology 2021Quote: ... The concentration of total protein mite extracts was determined using the Quick Start Bradford Protein Assay (Quick start Bradford 1x dye reagent, Bio-Rad, Cat# 500-0205), with Bovine Serum Albumin (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Then ran the assay in accordance with the specification (Bio-Plex Pro Mouse Cytokine Grp, Catlog NO. #M60009RDPD, LOT. 64212941, Bio-Rad Laboratories, Inc., USA). Read the plate at Bio-Plex MAGPIX System (Bio-Rad Laboratories ...
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bioRxiv - Developmental Biology 2020Quote: ... Bradford Protein assay method was used to determine the protein concentration using bovine serum albumin (BSA) as the standard (Bio-Rad catalog 500-0006). 1-20μg of tissue protein to be analyzed were combined with equal parts 2X Laemmli sample buffer (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: The supernatants of cell patches were collected after culturing under normoxic or hypoxic (5% O2) conditions and analysed using a fluorescence-dyed microsphere-based immune assay (Bio-Rad Laboratories, Hercules, CA) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were clarified for 5 min at 10,000 × g at +4□C and protein concentration was determined using DC Protein Assay (Bio-Rad Laboratories, Hercules, CA, USA). For immunoprecipitation ...
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bioRxiv - Neuroscience 2021Quote: ... to a final concentration of 10 mM and shielded from light for 30 minutes.We performed an automated detergent compatible protein assay (DCA) (Bio-Rad, Hercules, CA, Cat. #5000111), normalization (to 1 μg/μL ...
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bioRxiv - Cancer Biology 2020Quote: The ddPCRs assay comprised a total volume of 20 μL containing on the one hand the droplet Supermix (Bio-Rad Laboratories, Richmond, CA, USA) at final concentration of 500 nM of forward and reverse primers and 250 nM of VIC- and FAM-labeled probes and in the other hand the DNA input containing 8 µL of eluted cfDNA (mean ...
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bioRxiv - Developmental Biology 2021Quote: ... Quantification of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) reference gene RNA level was performed to determine the efficiency of RNA extraction and qPCR amplification (Bio-Rad PrimePCR assay #qHsaCED0038674). SARS-CoV-2 viral loads below 40 RNA copies/mL were categorized as undetectable and set at 1.0 log10 RNA copies/mL.
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bioRxiv - Genomics 2020Quote: ... and 250 nM TaqMan probe (Ref-HEX and assay specific-FAM dye labeled) in a 1× Bio-Rad Supermix (Bio-Rad, Hercules, CA, USA). The 20μl PCR reaction was mixed with Bio-Rad droplet generator oil and partitioned into 15,00020,000 droplets using the Bio-Rad QX-100 droplet generator (Bio-Rad) ...
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bioRxiv - Genetics 2020Quote: ... the soluble fraction was transferred to a new tube and total protein concentration was determined by DC protein assay (Bio-Rad, Hercules, CA, USA). Following quantification ...
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bioRxiv - Cancer Biology 2022Quote: ... The fluorescence intensity was calculated relative to protein concentration in each sample as assessed by the DC™ Protein Assay (BioRad, Hercules, CA, USA) according to manufacturer’s protocol.
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bioRxiv - Genomics 2022Quote: ... Protein concentration was quantified with the Quick Start Bovine Serum Albumin Bradford assay according to the manufacturer’s protocol (Bio-Rad Laboratories, Inc., Hercules, CA) and using an Eppendorf Biophotometer 6131.
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bioRxiv - Microbiology 2022Quote: Cytokine/chemokine concentration was assessed in cell culture supernatants 48 h post transfection by using immunoassays formatted on magnetic beads (Bio-Plex Pro Human Cytokine 27-plex Assay #M500KCAF0Y) (Bio-Rad, Hercules, CA, USA), according to the manufacturer’s protocol via Luminex 100 technology (Luminex ...
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bioRxiv - Neuroscience 2022Quote: Bilateral tissue punches were homogenized in 1% sodium dodecyl sulfate (SDS) processing buffer and protein was quantified using the Bio-Rad protein DC assay (Bio-Rad Laboratories, Berkeley, CA). For each sample ...
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bioRxiv - Microbiology 2024Quote: ... purity was checked by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in a 12% polyacrylamide gel using the Bio-Rad Protein Assay (Bio-Rad, Hercules, CA, USA), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... The AMR genotypes of transconjugant colonies were determined by polymerase chain reaction (PCR) assays on a T100™ Thermal Cycler (Bio-Rad, Hercules, CA). Reaction volumes were 25 µL and consisted of 12.5 µL of Accustart II PCR Toughmix (Quantabio ...
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bioRxiv - Physiology 2023Quote: Enzymes were extracted using a phosphate-based buffer (50 mM KH2PO4, 1 mM EDTA, 0.05 % Triton X-100) and quantified by Bradford assay (Bio-Rad #5000006, Bio-Rad, Hercules, CA, USA). Citrate synthase activity was measured spectrophotometrically as previously described106.
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bioRxiv - Biochemistry 2023Quote: ... The elutions were then concentrated on an Amicon spin column (10 kDa cutoff) and quantified by the DC assay (Bio-Rad, Hercules, CA, USA). The eluted protein was then diluted in PBS (pH 7.4) ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was used as cell extract for further experiments after determination of the protein concentration using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Cancer Biology 2024Quote: ... the protein concentration in the whole cell lysate or various subcellular fractions was determined by using the Bradford assay (Bio-Rad Laboratories, Hercules, CA). Immunoprecipitation was performed as previously described (3 ...
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bioRxiv - Biochemistry 2024Quote: ... purity was checked by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in a 12% polyacrylamide gel and their concentration quantitated using the Bio-Rad Protein Assay (Bio-Rad, Hercules, CA, USA), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Protein extracts were separated on 8 – 12 % polyacrylamide gels by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to PVDF membranes (Bio-Rad, Hercules, CA, USA, 0.2 µm pore size) in a wet transfer approach using the Mini Trans-Blot® cell (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: ... Blocking of the membrane was carried out by incubating in blocking solution for 1 h at room temperature (5% Bio-Rad Blotting-Grade Blocker in TSB-T buffer). Membrane was then incubated with anti-PcdA polyclonal antibody diluted 1:15,000 in blocking solution for 2 h at room temperature.
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Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: ... from ART or ART + GYY4137 treated groups were harvested to isolate total RNA using Qiagen RNAeasy isolation kit and 200 ng of total RNA was reverse transcribed (iScript™cDNA synthesis kit, Bio-Rad). Reverse transcribed cDNA was diluted 10-fold and amplified using primers against HIV LTRs and seminested – PCR was performed using primers and probe listed in Table S6 ...
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bioRxiv - Developmental Biology 2021Quote: ... using the whole SingleShot™ Cell Lysis Kit (SingleShot™ Cell Lysis Kit, 100 x 50 µl reactions Cat# 1725080, Bio-Rad) as follows ...
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bioRxiv - Physiology 2020Quote: ... RNA of treated cells was isolated using EZ-RNA isolation kit (Biological Industries Beit Haemek, Israel) and cDNA was synthesised using iScript cDNA synthesis kit (Bio-Rad, UK). Real-time qPCR analyses were performed on a StepOnePlus™ Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Genetics 2021Quote: ... we purified total RNA using the Qiagen RNeasy Mini kit (Cat#74106) and made cDNA using iscript cDNA synthesis kit from Biorad (Cat#1725036), followed by a Taqman assay to detect AHR transcription (Hs00169233-m1 for AHR expression and Hs0442063-g1 for GAPDH ...