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Citations for Bio-Rad :
851 - 900 of 1524 citations for 3 Chloro 2 Trimethylsiloxypropene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Equal numbers of cells were loaded onto the gel in 2× Laemmli Buffer (BioRad, #1610737), and proteins were loaded onto 18-well BioRad anyKD Criterion TGX gels (Bio-Rad ...
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bioRxiv - Developmental Biology 2024Quote: ... and the supernatant was mixed with equal volume 2 x Laemlli Buffer (Bio-Rad, 1610737) and heated to 95 °C for 5 min ...
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bioRxiv - Cell Biology 2024Quote: ... and the cell suspension was mixed with 50 µL molten 2% CleanCut agarose (Bio-Rad) after which the mixture was pipetted into a plug mold (Bio-Rad) ...
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Analysis of natural structures and chemical mapping data reveals local stability compensation in RNAbioRxiv - Biophysics 2024Quote: ... we first prepared a 2% agarose gel by diluting 10x TBE Buffer (Bio-Rad #1610770) to 1x with RNase-free water ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 μg of total RNA was used to make cDNA using iScript reverse transcriptase (BioRad) as per manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... 2 mM DTT and 0.5% OGNPG) on a Micro Bio-Spin 6 column (Bio-Rad) and then diluted to a concentration of 10 mg/ml ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was mixed with 5% w/v of SM-2 Biobeads (BioRad, 152-8920), cleaned with 2% TX-114 for 2 h and regenerated with 30 CV of methanol ...
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bioRxiv - Bioengineering 2024Quote: ... any remaining Triton 114 was removed using SM-2 bio-beads (1528920, Bio-Rad Laboratories). The phage was then purified via CsCl gradient ultracentrifugation (1.2–1.6 g/mL gradient ...
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bioRxiv - Bioengineering 2024Quote: ... 1-5 µg of target protein was resuspended in 2× Laemmli Sample Buffer (Bio-Rad) with adding 1:20 Beta-mercaptoethanol and 15 µL of the mixture was added onto Any kD™ Criterion™ TGX Stain-Free™ Protein gel (Bio-Rad) ...
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bioRxiv - Developmental Biology 2020Quote: ... Membranes were then washed 3 times for 5 min in TBST before detection using Clarity Western ECL Substrate (Bio-rad, 170-5061) and imaging on a ChemiDoc MP (Bio-rad ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were incubated for 1hr on ice with varying concentrations of fluorescently labeled hybridoma-derived or recombinant mAbs and then washed x3 in flow buffer (PBS + 3% fetal calf serum) prior to analysis on a ZE5 flow cytometer (BioRad, Hercules, CA). Equilibrium binding affinities were calculated via nonlinear regression (One site – total and non-specific binding ...
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bioRxiv - Molecular Biology 2020Quote: ... carried out in glass tubes filled with polyacrylamide gel using a mixture of ampholytes 3/10 and 5/8 (Bio-Rad, USA) overnight at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was detected by using a 1:1,000 anti-GAPDH hFAB™ Rhodamine antibody (Bio-Rad, Hercules, CA) in 5% milk TBST (Tris buffer saline plus Tween-20) ...
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bioRxiv - Microbiology 2022Quote: ... 300 nM concentration of each primer targeting the viral DNA substrate (5’- AGCGTGGGCGGGAAAATCTC-3’) and the indicated target DNA (table 1) and 1X iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories). The qPCR cycling conditions for quantifying INS activity included an initial incubation at 95°C for 3 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... 6 ng of cDNA was used per qPCR reaction consisting of gene-specific primers (Supplementary Information Table 3) and amplified with the PowerSYBR Green Master Mix (Applied Biosystems in a CFX-96 thermocycler (Bio-Rad). Data was acquired using the Bio-Rad CFX Manager 3.1 ...
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bioRxiv - Bioengineering 2022Quote: ... The PVDF membrane was then washed 3 × 10 min with TBST and incubated with horseradish peroxidase (HRP)-conjugated goat anti-mouse (diluted 1:20,000; Bio-Rad, Hercules, CA) secondary antibody for one hour at room temperature followed by 6 × 10 min washes with TBST and detection using the Radiance Plus chemiluminescence substrate (Azure Biosystems ...
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bioRxiv - Molecular Biology 2021Quote: ... Band intensities of at least 3 individual experiments were measured densitometrically and normalized to whole protein using the Image Lab Software 5.1 from BioRad (Biorad, Munich, Germany).
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bioRxiv - Biochemistry 2020Quote: ... N-glycans were reduced with 1 M NaBH4 in 50 mM KOH for 3 h at 50°C then desalted and enriched offline using AG 50W-X8 (Bio-Rad, Australia) strong cation exchange followed by porous graphitized carbon (PGC ...
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bioRxiv - Neuroscience 2022Quote: ... All reactions were performed using primers listed in the supplementary table 3 and the iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories). Relative expression levels were determined according to the ΔΔCt method with the human housekeeping gene ABELSON as endogenous control for normalization as previously described (Beillard et al. ...
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bioRxiv - Physiology 2021Quote: ... 95°C for 20 s followed by 40 cycles at 95°C for 3 s and 60°C for 30 s using a real-time thermal cycler (CFX Connect; BioRad, Hercules, CA).
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bioRxiv - Immunology 2021Quote: ... cDNA synthesis was performed using 3 ng (neutrophils) and 10 ng (monocytes) of total RNA and iScript™ Reverse Transcription Supermix (Bio-Rad). Relative quantitation of mRNA expression levels was determined with CFX96 Touch™ Real Time PCR using SsoAdvanced™ Universal SYBR Green Supermix and PrimePCR™ Primers (Bio-Rad) ...
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bioRxiv - Genomics 2020Quote: ... (see Supplementary Table 3 for a full list of oligos used in the present study) into 384-well hard-shell PCR plates (Bio-Rad HSP3901) using Mantis liquid handler (Formulatrix) ...
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bioRxiv - Biochemistry 2022Quote: ... Enzyme purity and molecular weight were estimated using a 12% SDS-PAGE and mini-PROTEAN 3 system (BioRad, Hercules, California, United States). Gels were stained using PageBlue Protein Staining Solution (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were washed 3 times with ice-cold PBS and boiled at 90 °C in 2x Laemnli sample buffer (Bio-Rad 1610737) for 5 minutes the following day for elution.
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bioRxiv - Zoology 2023Quote: ... followed by 40 cycles at 95°C for 3 s and 60°C for 30 s using a real-time thermal cycler (CFX Connect; BioRad, Hercules, CA).
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bioRxiv - Biochemistry 2024Quote: ... were annealed at a ratio of 3:1 molar ratio Incubated at 93°C for 3 minutes and slowly cooling down in C1000 Touch™ Thermal Cycler (Bio-Rad). The pre-annealed RNA-TS (32 µM ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were prepared for gel electrophoresis by adding a volume containing 20 ug protein to 3 uL 1 M DTT and 7.5 μL 4X Laemmli sample buffer (BioRad Cat. No. 1610747) and topping up to 30 μL with deionized water ...
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bioRxiv - Biochemistry 2023Quote: ... The membranes were washed three times for 5 min each with TBST and incubated with 1 mL of chemiluminescent detection reagent (Cytiva, RPN2232) for 3 min before image acquisition using a ChemiDoc MP Imaging System (Bio-Rad Laboratories). Horseradish peroxidase (HRP)-conjugated antibodies against β-actin (Cell Signaling ...
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bioRxiv - Biochemistry 2023Quote: ... The membranes were washed three times for 5 min each with TBST and incubated with 1 mL of chemiluminescent detection reagent (Cytiva, RPN2232) for 3 min before image acquisition using a ChemiDoc MP Imaging System (Bio-Rad Laboratories).
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bioRxiv - Neuroscience 2023Quote: ... Sections were blocked using 5% BSA in PBS and incubated overnight at 4 °C with 1 or an appropriate combination of up to 3 of the following antibodies: rat anti-CD8 (1:500, catalog no. MCA609G; Bio-Rad Laboratories), rat anti-CD4 (1:1,000 ...
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bioRxiv - Systems Biology 2023Quote: ... was applied for 3 min before the membranes were imaged using the ChemiDocXRS+imaging system (Bio-Rad Laboratories, Inc., Hercules, CA, USA). Quantification of proteins was conducted using Image J software (Version 1.52a ...
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bioRxiv - Immunology 2024Quote: ... The membranes were washed five times for 5 min each with TBST and incubated with 1 mL of chemiluminescent detection reagent (Cytiva, RPN2232) for 3 min before image acquisition using a ChemiDoc MP Imaging System (Bio-Rad Laboratories). Horseradish peroxidase (HRP)-conjugated antibodies against β-actin (Cell Signaling ...
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bioRxiv - Developmental Biology 2024Quote: ... and electroporated (square wave, 6 pulses, 30 V, 3 ms duration, 100 ms interval) using a gene Pulser XCell electroporator (Bio-Rad, 1652660). Zygotes were recovered and transferred to fresh KSOM drops and cultured overnight ...
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bioRxiv - Plant Biology 2024Quote: ... The protein extracts were resolved by 12% (w/v) SDS-PAGE using a Bio-Rad Mini PROTEAN 3™ electrophoresis unit (Bio-Rad) and electrotransferred onto Hybond ECL nitrocellulose sheets (GE Healthcare ...
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bioRxiv - Biochemistry 2024Quote: ... Each sample was then boiled for 3-5 min at 95 ºC and resolved on a 4-20 % gradient SDS-PAGE gel (TGXTM, Bio-Rad, 4561096) at 180 Volts for ∼30 min ...
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bioRxiv - Biochemistry 2024Quote: ... The primer sequences for detecting ACTB are 5’-GACCTGACTGACTACCTCAT-3’(forward) and 5’-TCTCCTTAATGTCACGCACG-3’ (reverse) Quantitative PCR (qPCR) reactions were conducted using the SYBR green PCR master mix (Bio-Rad, 1725121) in CFX96 Touch Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... Gel was transferred at 350 Amperes for 120 minutes onto pre-activated polyvinylidene difluoride (PVDF) or Nitrocellulose membranes in a Mini-Protean® 3 Cell (BioRad Laboratories). Correct protein transfer was checked by red Ponceau S Solution (Panreac) ...
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bioRxiv - Cell Biology 2024Quote: ... and then transferred onto a polyvinylidene fluoride (PVDF) membrane at constant voltage of 25V for 3 min using Trans-Blot TurboTransfer Packs (Cat.: 1704156, Lot.: 64340580, Bio-Rad, USA) and Trans-Blot Turbo system (Cat. ...
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bioRxiv - Cell Biology 2020Quote: ... destained for 2 h at room temp (Coomassie Brilliant Blue R-250 staining kit, BIO-RAD), and imaged on a ChemiDoc MP (BIO-RAD).
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bioRxiv - Molecular Biology 2020Quote: ... The mixture was then treated with 5% volume of Bio-Bead SM-2 resin (Bio-Rad) with shaking on ice for 15 min ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 specific genome levels were measured by qPCR using SsoFast EvaGreen supermix (Bio-Rad) according to manufacturer’s protocol.
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bioRxiv - Biochemistry 2020Quote: ... Biotinylated small RNAs were separated from HPDP-biotin and pyridine-2-thione using spin columns (BioRad) in ultra-pure water.
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bioRxiv - Developmental Biology 2020Quote: ... We ran the qPCR reactions using 1-2 µl cDNA in SYBR Green Master Mix (Biorad) totaling 20 µl in a Biorad CFX96 machine using 60°C as the annealing temperature ...
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bioRxiv - Bioengineering 2021Quote: ... and the selected primers (Table 2) using a CFX Connect™ Real-Time System (Bio-Rad) under the following conditions ...
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bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies were used: anti–α-tubulin (MCA77G YL1/2, rat monoclonal; Bio-Rad), anti-GAPDH (mouse ...
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bioRxiv - Cell Biology 2021Quote: Samples for electrophoresis were prepared by mixing with 2× Laemmli sample buffer (Bio-rad, Hercules, California) and denatured at 55°C for 5 min ...
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bioRxiv - Microbiology 2020Quote: ... in a 100 μL reaction for 2 hours and purified using P-30 spin columns (BioRad). The radiolabeled probe was heated to 100°C for 2 minutes and resuspended in 10 mL of ULTRAhyb Oligo hybridization buffer (Ambion) ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was synthesised from 2 µg purified RNA using iScript™ cDNA Synthesis Kit (Bio-Rad) and quantified by qRT-PCR using iTaq™ Universal SYBR® Green Supermix ...
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bioRxiv - Cancer Biology 2020Quote: ... Two percent agarose gel was prepared by dissolving 2 g Agarose (Bio-rad, Cat No. 1613102) and 2 µL SYBR Safe DNA Gel Stain (Invitrogen ...
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bioRxiv - Developmental Biology 2022Quote: ... Supernatant fractions were collected by centrifugation and boiled with 2× Laemmli sample buffer (Bio-Rad, 1610737) at 95 °C for 8-10 min ...