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Citations for Bio-Rad :
1001 - 1050 of 1524 citations for 3 Chloro 2 Trimethylsiloxypropene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... SARS-CoV-2 RT-qPCR reactions (10 µL) using the iTaq Universal Probe One-Step Kit (BioRad) and SARS-CoV-2 N1 primers/probe mix (Stock# 10006713 ...
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bioRxiv - Cancer Biology 2023Quote: Cells were lysed in sample buffer (2× Laemmli sample buffer, No. 1610737, Bio-Rad, Hercules, California, USA). Whole-cell lysates were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Physiology 2023Quote: ... Captured complexes were dissociated from the beads in the ×2 Laemmli buffer (Bio-Rad Laboratories, Hercules, CA) and analyzed by SDS-PAGE/western blotting using chemiluminescent substrate (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary antibody staining was done in 2% milk-TBST (+ 0.01% sodium azide) and detected using ChemiDoc (Biorad).
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bioRxiv - Neuroscience 2023Quote: ... or anti-rat TCRαβ antibody (2 µg/rat dissolved in saline solution, clone R73, Bio-Rad #MCA453GA) from day 9 to day 14 to deplete DRG T cells as previously described 66 ...
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bioRxiv - Microbiology 2024Quote: ... Pellets were resuspended to OD 10 in 1X Laemmeli Buffer supplemented with 2-mercaptoethanol (10%) (Bio-Rad) and boiled at 99°C for 10 minutes ...
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bioRxiv - Immunology 2024Quote: ... cells were stimulated with 5 μg/ml anti-Igκ plus anti-Igλ F(ab’)2 antibodies (BioRad). After 5 min of data acquisition ...
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bioRxiv - Neuroscience 2024Quote: ... with 10% 2-mercaptoethanol and ran on precast Any kD SDS-PAGE protein gels (BioRad, cat. # 4569034). Proteins were then transferred onto low-fluorescence PVDF membrane (BioRad cat ...
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bioRxiv - Neuroscience 2024Quote: ... was mechanically homogenized in Bioplex cell lysis buffer containing factors 1 and 2 (Bio-Rad, CA, USA) and centrifuged at 4500xg ...
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bioRxiv - Plant Biology 2024Quote: ... 5 µL of Precision Plus Dual Xtra Protein Standards (2–250 kDa) were used (Bio-Rad Laboratories). Oligomerization patterns were visualized using silver staining.
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was added to sample loading buffer with 1% (v/v) 2-mercaptoethanol (1610710, Bio-Rad). Samples were boiled at 95 degrees C for 5 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... Nanodisc formation was initiated by adding 320 mg/mL equilibrated Bio-Beads SM-2 resin (Bio-Rad), followed by constant agitation overnight at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... Quantitative PCR (qPCR) was performed with primer sets corresponding to primer list (Table 3) and using iTaq Universal SYBR Green Supermix (Bio-Rad, Cat. No. 172-5125) on an Applied Biosystems 7500 Fast Real-Time PCR system or ThermoFisher Scientific QuantStudio 3 or 5 Real-Time PCR system ...
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bioRxiv - Biochemistry 2022Quote: ... The plate was centrifuged at 2000rpm (3 minutes at room temperature) and placed in the CFX Touch™ Real Time PCR Detection System (Bio-Rad; Hercules, California, USA) set for an initial denaturation (95°C ...
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bioRxiv - Plant Biology 2022Quote: ... Amplification of target amplicons with the addition of 5’ XbaI and 3’ BamHI restriction enzyme digestion sites was carried out on a Bio-Rad T100 thermocycler (Bio-Rad Laboratories, Hercules CA, USA) via Bio-Rad iProof High Fidelity DNA Polymerase using manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Aliquots of clarified lysate (3 x 106 parasite equivalents) were resolved by SDS-PAGE (Mini-PROTEAN®TGX protein gel; Bio-Rad, Hercules, California, USA), transferred to PVDF membrane (Immobilon®-FL ...
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bioRxiv - Cell Biology 2023Quote: ... The membrane was washed again with 1x PBS-T (0.075 %) 3 times 10 minutes each and incubated with 1:1 ECL chemiluminescence solution (Clarity Western ECL, #170-5061, Bio-Rad Laboratories, Hercules, CA, USA). Signal was detected using an Amersham AI600 imager (Supplementary Table 5).
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bioRxiv - Microbiology 2024Quote: ... Then, 3 mL of each culture were pelleted (11,000 rpm, 1 min) to perform total RNA extraction (using BioRad’s Aurum™ Total RNA Mini Kit). RNA concentration and purity were assessed using a nanodrop and all samples shown to have A260/A280 and A260/A230 ratios ≥ 2 ...
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bioRxiv - Cell Biology 2020Quote: ... A reaction solution of 20 Δl comprising 10 μl 2× Sso Advanced universal SYBR Green Supermix (Bio-Rad), 1 μl cDNA template (25 ng) ...
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Control of cortical cytoskeleton-membrane interaction by RhoA regulates peripheral nerve myelinationbioRxiv - Neuroscience 2021Quote: ... protein extracts were prepared in lysis buffer with 2% SDS and resolved in a 4-12% gel (Biorad). Membranes were probed overnight at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... After 2 weeks cells were stained by the sulforhodamine B and photographed by E-Gel Imager (Bio-Rad). The cloning numbers in each group were counted to calculate the inhibition rate.
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bioRxiv - Molecular Biology 2021Quote: ... The qPCR experiments were performed according to an absolute quantification design on the Opticon 2 PCR machine (Biorad). Β2Microglobulin (B2M ...
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bioRxiv - Cancer Biology 2021Quote: 2 ⨯ 106 CAR T cells collected after 24-day expansion were resuspended with 1 ⨯ Laemmli sample buffer (BIORAD) containing 2.5% β-mercaptoethanol before sonication with 50% amplitude in Sonic Dismembrator (FisherBrand) ...
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bioRxiv - Bioengineering 2021Quote: ... First strand cDNA was synthesized utilizing 2 µg of total RNA and iScriptTM cDNA Synthesis Kit (Bio-Rad). A fraction (0.75 μg ...
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bioRxiv - Bioengineering 2021Quote: ... 2 uL of bacterial culture was mixed with 10 uL 2X Sso Fast Evagreen qPCR mixture (BioRad USA). The primers were mixed into an equal forward/reverse (F/R ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 μg of total RNA was used for cDNA preparation using the iScript cDNA synthesis kit (Bio-Rad). A 1/10 dilution of cDNA was used for qRT-PCR analysis using the FastStart Universal SYBR Green Master Mix (Roche) ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were transferred to a nitrocellulose membrane for 2 hours in a wet blot tank system (Bio-Rad), and membranes were blocked with 5% skim milk for 30 min at room temperature before incubating with primary antibody ...
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bioRxiv - Microbiology 2021Quote: ... using primers specific for fathead minnow β-actin (Table 2) with iTaq universal SYBR green supermix (Bio-Rad).
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bioRxiv - Molecular Biology 2020Quote: ... IPG strips were run upon SDS PAGE (12.5% acrylamide) using the Protean® II 2-D Cell (BioRad). Gels were then Coomassie blue stained (Phastgel Blue R ...
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bioRxiv - Bioengineering 2022Quote: ... ddPCR reactions were set up as follows: 11 μL 2×ddPCR™ supermix for probes (no dUTP) (BioRad), 0.2 μL FWD primer (100 μM) ...
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bioRxiv - Biochemistry 2022Quote: ... PCR products were confirmed by 2% agarose gel electrophoresis stained with GelRed using a 50 bp marker (BioRad). PCR products were normalized using Mag-bind Total Pure NGS ...
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bioRxiv - Cancer Biology 2022Quote: ... 2×106 cells (extracted tissues or cell culture) were suspended in 50 μL RIPA (Radio ImmunoPrecipitaion Assay; BioRad) lysis buffer containing 200 mM PMSF (Alpha-Phenyl Methyl Sulfonyl Fluoride ...
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bioRxiv - Genomics 2022Quote: ... These products were analyzed on a 2% agarose gel and was imaged with Gel Doc imaging system (BioRad). The observation of multiple bands implied successful targeting of the sequences by the sgRNA.
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bioRxiv - Microbiology 2022Quote: ... Quantitative PCR was performed on 2 μl with the SsoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad) and the following primer pairs ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were resuspended in agarose insert buffer and mixed 1:1 with 2% low-melting agarose (Bio-Rad). Gel inserts were solidified at 4°C for overnight and were stored in agarose insert buffer until analysis ...
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bioRxiv - Biochemistry 2020Quote: ... Both PK-treated and untreated samples were then mixed 1:2 with 4X Laemmli sample buffer (Bio-Rad) containing DTT ...
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bioRxiv - Developmental Biology 2020Quote: ... Smit1/2 and TonEBP were measured by real time qPCR using SYBR Green I (Bio-Rad, Mississauga ON) and 5 μM of forward and reverse primer mix (Bio-Rad CFX384 ...
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bioRxiv - Genetics 2021Quote: ... The enriched proteins were dissociated by the addition of 2 x Laemmli sample buffer (161-0737, Bio-Rad) and boiled at 95 °C for 10 min ...
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bioRxiv - Biochemistry 2021Quote: KirBac3.1 protein was buffer-exchanged to 200 mM ammonium acetate (pH=8.0) with 0.5% C8E4 (2×CMC) using a Biospin-6 column (BioRad). The protein sample was loaded into a gold coated needle prepared in-house and analysed on a modified Q-Exactive mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... Gels were dried for 2 h at 80°C and analysed on a Personal Molecular Imager (Bio-Rad). For RNA stability assays ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 μl of RNA were mixed with 2 μl of 50 μM oligo dT primer (Bio-Rad, 1725038) and 1 μl of 25 mM dNTP mix (NEB ...
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bioRxiv - Cell Biology 2020Quote: ... anti-α-tubulin YL1/2 from rat (MCA77G, Bio-Rad AbD Serotec, at 1:2000 dilution for IF), anti-rabbit Alexa Fluor 488 (A11008 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 2 μl of the cDNA sample were supplemented with 5 μL of SsoADV Universal SYBR Green Supermix (BioRad) mix 2X ...
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bioRxiv - Plant Biology 2022Quote: ... For α-ACTIN-2 the secondary antibody Goat Anti-Mouse IgG (H + L)-HRP conjugate (1706516, Bio-rad) was used at a dilution of 1:5000 ...
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bioRxiv - Molecular Biology 2023Quote: ... Captured proteins were separated from beads by incubating beads in 50 μl 2× Laemmli Sample Buffer (Bio-Rad) at 95°C for 25 min ...
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bioRxiv - Cell Biology 2023Quote: Protein samples were added with equal volume of 2× Laemmli sample buffer (Bio-Rad Laboratories, Hercules, CA, USA) containing 65.8 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 μg of total RNA was used for cDNA preparation using the iScript cDNA Synthesis Kit (Bio-Rad). A 1/10 dilution of the cDNA was then used for qRT-PCR analysis utilizing the SYBR Green PCR Master Mix (Applied Biosystems) ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was passed twice on 2 x Mini CHT ™ type I column (Bio-Rad, United States). Elution was performed with a gradient of 0 to 200 mM NaPO42- in 10 min ...
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bioRxiv - Physiology 2023Quote: ... Protein samples were suspended in Laemmli Sample Buffer supplemented with 2-Mercaptoethanol (β-mercaptoethanol) (BioRad Hercules, California – USA). Then ...
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bioRxiv - Immunology 2022Quote: ... 200 μL of this transformation mix was then aliquoted into pre-chilled 2 mm electroporation cuvettes (Bio-Rad) and electroporated at 1500 V with an average time constant of ∼4.5 ms using a Gene Pulser Xcell Electroporation System (Bio-Rad) ...