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Citations for Bio-Rad :
1101 - 1150 of 1524 citations for 3 Chloro 2 Trimethylsiloxypropene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... The SARS-COV-2 NTD expression was analysed with SDS-PAGE followed by Western-blot on PVDF membrane (Biorad) using α-strep-tag mouse antibodies 1:3000 (IBA Life Sciences) ...
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bioRxiv - Microbiology 2023Quote: ... 2 min at 72°C for final extension in a T100 Thermal Cycler (BIO-RAD Lab. Inc., Hercules, CA). The sizes of PCR products were checked by running agarose gel electrophoresis ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with 2% w/v CA and treated with (7% w/v) Chelex100 resin (Bio-Rad, Mississauga, ON, Canada) at 4°C overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... and 2 μL cDNA template were performed in technical duplicate wells on a C1000 Touch Thermal Cycler (Bio-Rad) with a CFX96 Touch Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... RT reactions were diluted 2-fold and qPCR was performed in duplicate with SsoAdvanced SYBR Green Supermix (Bio-Rad) according to manufacturer’s protocols with qPCR_FWD and qPCR_REV primers from IDT (see below) ...
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bioRxiv - Developmental Biology 2024Quote: ... centrifuged at 14,000rpm at RT for 2 minutes and separated on precast 10% SDS-PAGE gels (4561036, Bio-Rad) at 100V for 70 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... Immunoprecipitants were then gently washed three times with KPBS and eluted with 2 x Laemmli sample buffer (Bio-Rad) and subjected to immunoblot analysis.
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bioRxiv - Evolutionary Biology 2023Quote: ... 1×108 concentrated fungal protoplasts (37 μL) (64) were mixed with 2% CleanCut Agarose (63 μL, Bio-Rad, #1703594). The mixture was then loaded into a plug mold (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... the concentration was calculated by the Bradford assay using a Quick Start Bradford Protein Assay Kit 2 (Bio-Rad).
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bioRxiv - Systems Biology 2023Quote: ... The heart tissue samples were then combined with 2 x Laemelli buffer (Bio-Rad Laboratories, Inc., Hercules, CA, USA). Protein samples were then separated on 7.5 to 20% v/v sodium dodecyl sulfate (SDS ...
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bioRxiv - Microbiology 2023Quote: ... using 2 mm electroporation cuvettes (Cell Projects, EP-102) and electroporation program Ec2 (V = 2.5 kV) with MicroPulser Electroporator (Biorad). Plasmid sequences were confirmed by sanger sequencing.
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bioRxiv - Microbiology 2023Quote: ... Each 100 μl aliquot was transformed with 0.1 pmol of EZ-Tn5
Tnp Transposome using 2 mm electroporation cuvettes (Cell Projects, EP-102) and electroporation program Ec2 (V = 2.5 kV) with MicroPulser Electroporator (Biorad). Each reaction was allowed to recover in 500 μl SOC (2% tryptone ... -
bioRxiv - Immunology 2023Quote: Diluted mouse serum (1:100 in 0.1% BSA in PBS) was incubated on HEp-2 slides (MBL or BioRad) for 1 hour in the dark at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... MEC samples were directly homogenized using 2x Laemmli Sample Buffer and supplemented with 2-mercaptoethanol (β-mercaptoethanol) (BioRad Hercules). Proteins were separated by electrophoresis on a polyacrylamide gradient gel (BioRad Hercules ...
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bioRxiv - Biophysics 2023Quote: ... slides were prepared with unlabeled microtubules attached to the slide surface via an anti-tubulin antibody (BioRad YL1/2). Kinesin-liposome complexes were added to the flow cell and imaged in TIRF ...
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bioRxiv - Biophysics 2023Quote: ... Bio-Beads SM-2 (#152-3920) and the enhanced chemiluminescence (ECL) western blotting reagent (#170-50001) from Bio-Rad Laboratories (Hercules ...
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bioRxiv - Cancer Biology 2024Quote: ... slides were incubated at 60°C for 2 h on a Xenium thermocycler adaptor plate (BioRad, C1000 Touch Thermocycler), followed by deparaffinized through a series of immersions in xylene ...
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bioRxiv - Biochemistry 2024Quote: A 22ul reaction mixture was prepared comprising 11ul 2×ddPCR Supermix for probes (no dUTP) (Bio-Rad, CA, USA), 0.55 ul of TRECs primers (36uM stock ...
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bioRxiv - Cell Biology 2024Quote: ... Immunoprecipitants were then gently washed three times with KPBS and eluted with 2 x Laemmli sample buffer (Bio-Rad) and subjected to immunoblot analysis.
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bioRxiv - Immunology 2024Quote: ... The SARS-CoV-2 spike proteins and pseudotype virus capsid were visualized using a ChemiDoc MP imaging system (Biorad) using a rabbit anti-Spike polyclonal (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... The generated cDNAs were then diluted (2×) and combined with the primer set and SYBR Green Supermix (Bio-Rad) following the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2024Quote: ... slides were incubated at 60°C for 2 h on a Xenium thermocycler adaptor plate (BioRad, C1000 Touch Thermocycler), followed by deparaffinized through a series of immersions in xylene ...
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bioRxiv - Microbiology 2022Quote: ... and 1’,3’-bis[1-palmitoyl-2-oleoyl-sn-glycero-3-phospho]-glycerol (16:0-18:1 Cardiolipin) were spotted using a Hamilton syringe onto a nitrocellulose membrane (Biorad trans-blot turbo RTA Midi 0.2 µm) to yield 10 ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were prepared at 25 µg protein/well with 2X laemmli loading buffer and 2-mercaptoethanol and run on pre-cast 4-15% gels (Biorad). Proteins were wet transferred onto PVDF membranes ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were pelleted and washed once with 0.75 ml of ice cold 0.5% TCA and resuspended in 2× Laemmli sample buffer (Bio-Rad Laboratories). Western blot was performed as described (Hsu et al. ...
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bioRxiv - Immunology 2021Quote: ... sections were stained with 0.4 µg/mL rabbit -anti-human CD20 polyclonal antibodies (Neomarkers) and 2 µg/mL rat-anti-human CD3 antibodies (clone MCA1477, BioRad). Then ...
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bioRxiv - Cancer Biology 2020Quote: ... ran at 120 V for ∼2 h and transferred at 100 V for 1 h onto nitrocellulose membranes (Bio-rad) using the wet blot method ...
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bioRxiv - Cancer Biology 2021Quote: ... DNA was diluted to 10 ng/µland 2 µl was used in each RT-qPCR reaction with 10 µl iTAQ Universal SYBR green supermix (BioRad) and 400 nM primers ...
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bioRxiv - Cell Biology 2020Quote: ... Detergent was removed by three addition of BioBeads SM-2 adsorbent resin (25 mg per 1 mL of lipid mixture) (BioRad) for 2 h each at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... proteins were transferred onto 0.2 µm pore nitrocellulose membranes for 10 min at 2.5 V using the Trans-blot turbo transfer system (Bio-Rad). For more sensitive detection of neurexin levels ...
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bioRxiv - Cell Biology 2021Quote: ... 15% for FKB-2) and transferred onto nitrocellulose membranes for 1 hour at 200 mA (SemiDry transfer blot, Bio-Rad). After incubation with blocking solution (LICOR Biosciences ...
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bioRxiv - Molecular Biology 2020Quote: ... 95°C), and run (loaded in 2 wells, 20 μL per well) on a 10 well 10% TBE-urea gel (Biorad) (200 V ...
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bioRxiv - Molecular Biology 2020Quote: ... 2.5 mg/ml Orange G), denatured (2 min, 80°C) and run on a 10 well 10% TBE-urea gel (Biorad) (200 V ...
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bioRxiv - Biochemistry 2022Quote: ... The co-IP samples and input (1% of whole cell lysates) were boiled in 2× Laemmli sample buffer (Bio-Rad) supplemented with 2-mercaptoethanol (Bio-Rad ...
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bioRxiv - Biochemistry 2020Quote: ... Plates were imaged together with a 1 cm2 reference area on black background (see example in Supplementary Data 2) using a Gel-Doc XR + gel documentation system (BioRad). Images were analysed in MATLAB using the code in Supplementary Note 2 to determine the growth area (in cm2 ...
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bioRxiv - Neuroscience 2021Quote: MV pellets were sonicated three times for 10 s at 20 Hz (Vibra cell VCX130) in 2% SDS and heated at 56°C in Laemmli loading buffer (Biorad). The protein content was measured using the Pierce 660 nm protein assay kit (Thermo Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... The input (15 µg) and eluted proteins (∼2% of IPT) were fractionated in 4−15 % SDS-PAGE gels (Bio-rad) and analyzed by immunoblotting ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... containing 2 μl of five times diluted cDNA and monitored by CFX Connect Real-Time PCR Detection System (Bio-Rad). The following cycling parameters were used ...
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bioRxiv - Microbiology 2020Quote: ... at 1/10,000 dilution in TBE buffer for 2 h with rocking and visualised using a Gel Doc EZ Imager (Bio-Rad). Band intensity was quantified using ImageJ software ...
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bioRxiv - Plant Biology 2021Quote: ... Quantification of relative gene expression was performed using the comparative CT method (2-ΔΔCt) (Schmittgen & Livak, 2008) on a CFX96 Real-Time System (BioRad). Primers used are listed in Supplemental Table 1.
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bioRxiv - Plant Biology 2021Quote: ... Quantification of relative gene expression was performed using the comparative CT method (2−ΔΔCt) (Schmittgen & Livak, 2008) on a CFX384 Touch Real-Time PCR Detection System (BioRad).
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were washed in PBS-T and developed for 2 minutes with ClarityTM Western ECL Substrate (Bio-Rad, CA, USA). Imaging was performed using a ChemiDocTM MP Imaging System with Image LabTM software (Bio-Rad ...
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bioRxiv - Genomics 2021Quote: ... at 1.8 kV distributed over 2 cuvettes (0.1 cm gap width) per tube using a Micropulser Electroporator (Bio-Rad Laboratories). 10-fold serial dilutions of a 10 μL aliquot were plated on LB plates with ampicillin at 100 μg/mL to assess electroporation efficiency ...
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bioRxiv - Cancer Biology 2022Quote: ... and 2 µl of the lysates were subject to 1 ml of 0.2x Bradford protein dye reagent (Bio-Rad #5000006) for protein quantification ...
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bioRxiv - Microbiology 2022Quote: ... Lysates were centrifuged for 15 min at 10,000 x g at 4 °C and supernatants were collected for protein quantification using the Quick start Bradford protein assay kit 2 (Biorad). Samples were mixed with Laemmli buffer (Biorad ...
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bioRxiv - Pathology 2022Quote: ... The CD68 primary antibody incubation was performed at room temperature for 2 hours (mouse CD68, BIO-RAD, MCA1957, 1:750) and CD11b staining (BD Pharmigen ...
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bioRxiv - Neuroscience 2022Quote: ... 2 ng of ExoDNA was used to calculate mtDNA copies number with iQ™ SYBR® Green Supermix (Bio-Rad) on a CFX96 Touch™ Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Developmental Biology 2022Quote: Whole-cell lysates for immunoblotting were prepared by dissolving cells in Laemmli Sample Buffer containing 5% 2-mercaptoethanol (Bio-Rad). Nuclear and cytosolic fractions of iPSCs-differentiated cells were acquired using NE-PER™ Nuclear and Cytoplasmic Extraction Reagents (Thermo Scientific #78833 ...
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bioRxiv - Microbiology 2022Quote: ... Viral copy numbers were assessed via digital-droplet PCR (ddPCR) using SARS-CoV-2 ddPCR kit (#12013743) from Bio-Rad with N1 and N2 primers and probes targeting the nucleocapsid genes ...
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bioRxiv - Microbiology 2020Quote: ... Protoplast suspensions (2 × 108 cells/ml) containing 0.6% low melting agarose gel were added to 50-well dispensable mold plates (Bio-Rad). Plugs were immersed in 10 ml of NDS (1% N-lauroyl sarcosinate sodium salt solution ...