Labshake search
Citations for Bio-Rad :
8651 - 8700 of 9281 citations for Nuclear Protein Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... The lysates were centrifuged at 18,000 x g for 30 minutes at 4 °C and the supernatant was incubated with SurebeadsTM Protein A Magnetic Bead (Bio-rad) conjugated with rabbit monoclonal DYKDDDDK antibody at RT for two hours ...
-
bioRxiv - Neuroscience 2023Quote: ... 10-30 μg of protein extracts were run at 100V for 1 hr on 10% pre-cast SDS-PAGE gels (Bio-Rad), then electro-transferred to 0.2 μm nitrocellulose membranes using the Trans-Blot Turbo Transfer system (Bio-Rad) ...
-
bioRxiv - Cell Biology 2023Quote: ... A total of 20 μg protein was denatured at 95 °C for 5 min and loaded on a 4-20% precast polyacrylamide gel (BioRad, 4568094) for gel electrophoresis ...
-
bioRxiv - Neuroscience 2023Quote: ... The supernatants were collected from human or mouse brain homogenates after centrifugation and the protein concentration was measured by Bradford Assay (Bio-Rad). Equal amounts of proteins were run on a 4-12% gradient SDS-PAGE gel (Invitrogen ...
-
bioRxiv - Immunology 2023Quote: ... 1min activation of the gel and imaging of total protein on the blot was performed by a Gel Doc EZ Imager (Bio-Rad). Blots were blocked in Intercept blocking buffer (PBS ...
-
bioRxiv - Genetics 2023Quote: ... The gels were run until proper band separation was achieved and the protein was transferred onto a 0.2 µm nitrocellulose membrane using the wet-transfer method (Bio-Rad Laboratories). The membranes were blocked with Odyssey Blocking Buffer (LiCOR Biosciences ...
-
bioRxiv - Microbiology 2023Quote: ... 10% ß-mercaptoethanol) was used as the starting material for separation on 4–20 % Mini-PROTEAN TGX Stain-Free Protein Gels (Bio-Rad). The purified virus was loaded into the sample well and was run on the gel placed in a Mini-PROTEAN Tetra Vertical Electrophoresis Cell for 30 min at 200 V ...
-
bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by isoelectric focusing on immobilized linear pH gradient strips (pH 5 to 8, Bio-Rad, Hercules, CA, USA) before separation by SDS-PAGE ...
-
bioRxiv - Microbiology 2023Quote: ... filtered HEK293F supernatants containing the expressed proteins were flowed (0.5 -1.0 mL/min) over Econo-Column chromatography columns (Bio-Rad, CA, USA) containing 1-2 mL of agarose bound GNL (Vector Laboratories) ...
-
bioRxiv - Pathology 2023Quote: ... total proteins were extracted and examined as previously described.59 The blotting was performed on the PVDF membrane (Bio-Rad, USA) and imaged with the Western Blotting Detection Kit (Advansta ...
-
bioRxiv - Cell Biology 2023Quote: ... Band intensities were normalized to total protein in the sample quantified within each lane using Stain-Free Dye Imaging (Bio-Rad).
-
bioRxiv - Cancer Biology 2023Quote: ... Sodium-dodecyl-sulfate polyacrylamide gel electrophoresis and transfers of proteins to .45uM nitrocellulose membranes were performed using the Protean 2 or 3 systems (Bio-Rad) and protocols provided by the manufacturer ...
-
bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by SDS-PAGE and transferred onto PVDF membranes applying the Trans-Blot Turbo Transfer system (Bio-Rad Laboratories). Membranes were blocked with 5% BSA or 5% NFDM in 1X TBS-T buffer and incubated with specific primary and secondary antibodies ...
-
bioRxiv - Cancer Biology 2023Quote: ... Lysate samples with the same amount of total protein (40-50 μg) were mixed with 4x Laemmli Sample Buffer (Bio-Rad #161-0737 ...
-
bioRxiv - Bioengineering 2023Quote: ... 30μg of total protein from each sample was placed into Mini-PROTEAN (R symbol) TGX (™) 4-20% gels (Bio-Rad) and subjected to SDS-PAGE ...
-
bioRxiv - Bioengineering 2023Quote: Purified protein samples were visualized to evaluate purity by SDS-PAGE using the Bio-Rad mini-protean system (Bio-Rad laboratories). Samples were mixed in a 1:1 ratio with 2x Laemmli sample buffer containing 50 mM 1,4-dithiothreitol (DTT ...
-
bioRxiv - Microbiology 2023Quote: Proteins were separated by SDS-PAGE on 4-15% gradient Mini-Protean TGX Stain-free gels (cat. no. 4568083, Bio-Rad), transferred onto PVDF membranes (cat ...
-
bioRxiv - Microbiology 2023Quote: Total protein extracts (5x106 cells) were separated by SDS-PAGE (10%) and immunoblotted on TransBlot Turbo Midi-size PVDF Membranes (Bio-Rad). Immunodetection was performed using the primary antibodies ...
-
bioRxiv - Molecular Biology 2023Quote: 10-20% of the samples containing immunoprecipitated ECTs for mass-spectrometry were subjected to SDS-PAGE in 4-20% CriterionTM TGXTM Precast Midi Protein gels (Bio-Rad) for visual assessment ...
-
bioRxiv - Immunology 2023Quote: ... 8 µg protein per condition were separated by SDS-PAGE and blotted onto a 0.2 µm nitrocellulose membrane (Bio-Rad, #1704158) using a Trans-Blot® Turbo™ Transfer System (Bio-Rad) ...
-
bioRxiv - Immunology 2023Quote: Purified recombinant His-tagged proteins were diluted to 0.5µg/ml in either Laemmli sample buffer alone (Bio-Rad, Hercules, CA, USA) or containing 50 mM dithiothreitol (DTT ...
-
bioRxiv - Physiology 2023Quote: ... The protein extract (40μg) was separated on 8 or 10% polyacrylamide mini gel and transferred to a PVDF membrane (Bio-Rad, USA). Blotted membranes were incubated overnight at 4°C in SuperBlock(PBS ...
-
bioRxiv - Cancer Biology 2023Quote: ... The protein lysates were run on 10% sodium dodecyl sulfate-polyacrylamide gels (SDS-PAGE) and transferred to a nitrocellulose membrane (Bio-Rad) using a wet tank blotting system ...
-
bioRxiv - Biochemistry 2023Quote: ... Thermal stability of the protein samples was measured in a CFX96 Dx Real-Time qPCR instrument (Bio-Rad, Hercules, CA, USA); program ...
-
bioRxiv - Bioengineering 2023Quote: ... Equal amounts of the proteins (50 µg) were electrophoresed (125 volts, 1 h) on a 4-20% Mini protean TGX stain-free protein gel (Bio-Rad) and transferred onto nitrocellulose membranes (125 volts ...
-
bioRxiv - Cell Biology 2023Quote: ... 20-40 μg of total protein was resolved on 10% SDS-PAGE gels followed by immunoblotting on PVDF membranes (Bio-rad). Membranes were developed by chemiluminescence (SuperSignal West Pico ...
-
bioRxiv - Biochemistry 2022Quote: NKX2-5 homeodomain purity was evaluated through SDS-PAGE using 15 well Mini-PROTEAN TGX Precast Protein Gels® (Bio-Rad). Samples were prepared with 4X loading buffer containing β-mercaptoethanol (BME ...
-
bioRxiv - Cell Biology 2023Quote: ... for protein extracted from cultured cells or brain sections and micro bicinchoninic acid (microBCA) for protein extracted from EVs according to the manufacturer’s instructions (Bio-Rad Laboratories).
-
bioRxiv - Cancer Biology 2023Quote: ... and the samples were boiled for 5 minutes before being loaded on the gel (4-15% Criterion TGX Precast Midi Protein Gel, Bio-Rad). Proteins were separated by SDS-PAGE and blotted into a polyvinylidene fluoride (PVDF ...
-
bioRxiv - Cancer Biology 2023Quote: ... Protein samples were subjected to 10–12% SDS-PAGE and transferred to a polyvinylidene fluoride membrane (Bio-Rad, Hercules, CA, USA).
-
bioRxiv - Cell Biology 2023Quote: ... Protein content was confirmed by mixing 2 μL of each elution step with 200 μL Bradford solution (Protein assay dye reagent (Bio-Rad) diluted 1:5 in H2O) ...
-
bioRxiv - Molecular Biology 2023Quote: ... and Precision Plus Protein Unstained Standards (#1610363) was resolved by SDS-PAGE using precast 4-20% MiniProtean TGX Stain Free Gels (Bio-Rad). The blot in Fig ...
-
bioRxiv - Microbiology 2022Quote: ... and proteins were analysed either by western blot or by silver staining after electrophoretic separation on polyacrylamide SDS-PAGE gel (Bio-Rad 4–20% Criterion™ TGX Stain-Free™ Protein Gel ...
-
bioRxiv - Bioengineering 2023Quote: ... Approximately 5 x 108 vesicles and purified HaloTag standard curve were loaded and run on a 12% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad) at 150 V for 90 min or 50V for 10 min followed by 100V for approximately 70 min ...
-
bioRxiv - Microbiology 2023Quote: ... equivalent total protein quantities from membrane samples were resolved by SDS-PAGE using Any kDTM polyacrylamide gels (Bio-Rad, Hercules, CA). Proteins transferred to a nitrocellulose membrane were probed with a monoclonal anti-His6 antibody (Qiagen ...
-
bioRxiv - Microbiology 2023Quote: ... 25 µg or 50 µg of normalized protein for LapA-HA or MapA-HA tagged strains respectively was mixed with 4x Laemmli Sample Buffer (Bio-Rad) and 2-mercaptoethanol to a 1x working dilution and samples were boiled for 5 minutes ...
-
bioRxiv - Microbiology 2023Quote: ... targeting SARS-CoV-2 gRNA and N protein sgRNA were used for the quantification by RT-qPCR using iTaq Universal SYBER Green Supermix (Bio-Rad) and normalised to β-actin using 2-ΔΔCT.
-
bioRxiv - Molecular Biology 2023Quote: ... Protein concentration of the supernatant was measured using the detergent compatible (DC Lowry) protein assay kit according to the manufacturer′s directions (Bio-Rad). For each sample ...
-
bioRxiv - Cell Biology 2023Quote: ... Aliquots of cell lysates (40-60 μg of protein) were subjected to SDS/PAGE on an 8 to 15% (vol/vol) acrylamide gel (MiniProtean; Bio-Rad) including PageRuler Prestained Protein Ladder (Thermo) ...
-
bioRxiv - Physiology 2023Quote: ... The subsequent protein pellet was solubilized in 50 µL Laemmli sample buffer and 20 µg protein was run in a 12.5% SDS-Page gel (BioRad Criterion system). The gels were fixed and stained with Coomassie blue (GelCode blue ...
-
bioRxiv - Immunology 2023Quote: ... Even amounts of each protein sample were separated on a 8–10% polyacrylamide gel using Mini-PROTEAN Tetra Cell electrophoresis system (Bio-Rad). Upon completion of electrophoresis ...
-
bioRxiv - Neuroscience 2023Quote: ... Equal amounts of proteins (20–100μg as optimal for each antibody) for each sample was loaded into 4-15% Mini-PROTEAN TGX Stain-Free Protein Gels (BioRad; #4568084) for electrophoresis ...
-
bioRxiv - Neuroscience 2023Quote: ... Protein (10 ug) from each sample was treated with ß-mercaptoethanol and subsequently electrophoresed on precast 4%–10% gradient gels (Biorad), as previously described (Alcantara et al. ...
-
bioRxiv - Microbiology 2023Quote: ... This panel had been tested negative for SARS-CoV-2 nucleocapsid protein expression using Bio-Plex Pro Human IgG SARS-CoV-2 N/RBD/S1/S2 4-Plex Panel (Bio-rad). The second panel consisted of 20 sera from individuals who were previously infected by SARS-CoV-2 vaccinated with 2-4 doses of parental mRNA vaccine ...
-
bioRxiv - Plant Biology 2023Quote: ... 20 µl of input and 5 µl of IP samples were loaded and protein samples were separated on 4-20% Tris-glycine polyacrylamide gels (Bio-Rad) at 170 V for 1 hour in 1X Tris/glycine/SDS running buffer ...
-
bioRxiv - Plant Biology 2023Quote: ... The proteins were then transferred to polyvinylidene difluoride membranes using NuPAGE Transfer Buffer using a Trans-Blot Turbo Transfer System (Bio-Rad) as per the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... The proteins were denatured by boiling for 10 min in the DTT-containing sample buffer and separated by 4–20% Mini-PROTEIN TGX gels (Bio-Rad) and then transferred to the 0.2 µm polyvinylidene difluoride (PVDF ...
-
bioRxiv - Molecular Biology 2023Quote: ... resin with immunoprecipitated proteins were resuspended in SDS-loading buffer and electrophoresed on a 4-20% TGX stain-free gel (Bio-Rad) for 10 min ...
-
bioRxiv - Cancer Biology 2023Quote: ... Each sample was separated by non-reducing SDS-PAGE (6% acrylamide gel) with Precision Plus Protein Dual Color Standards (Bio-Rad) and transferred to nitrocellulose membrane overnight at 4 °C ...
-
bioRxiv - Cell Biology 2023Quote: ... 20-40 µg of total protein was resolved on 10% SDS-PAGE gels followed by immunoblotting on PVDF membranes (Bio-rad). Membranes were developed by chemiluminescence (SuperSignal West Pico ...