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Citations for Bio-Rad :
8501 - 8550 of 9281 citations for Nuclear Protein Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Protein content was determined by BCA assay (Pierce) and proteins were separated by SDS-PAGE and transferred onto nitrocellulose membrane (Bio-Rad). Membranes were blocked ...
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bioRxiv - Cell Biology 2021Quote: ... Protein content was measured by Bradford assay and 30 mg of protein per sample were loaded into 4-20 % precast SDS-PAGE gels (Bio-Rad) under denaturing conditions ...
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bioRxiv - Physiology 2020Quote: ... Twelve micrograms of protein were subjected to SDS-PAGE on 4-20% Criterion TGX stain-free gels (Bio-Rad, Hercules, CA) and transferred to polyvinylidene diflouride membranes (PVDF ...
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bioRxiv - Developmental Biology 2021Quote: Proteins extracted from Padi4CTL and Padi4CKO were subjected to SDS–PAGE (4–20% Mini-PROTEAN® TGX™ Precast gel, Biorad) and then transferred onto a nitrocellulose membrane ...
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bioRxiv - Microbiology 2021Quote: ... the relative amount of each protein was calculated using Quantity One software (densitometer GS-800; Bio-Rad Laboratories, Hercules, CA, USA), and was presented as a percentage of total protein [25].
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bioRxiv - Bioengineering 2021Quote: ... proteins were transferred to a polyvinylidene fluoride (PVDF) membrane (Cytiva) using a Trans-Blot SD Semi-Dry Electrophoretic Cell (Bio-Rad) with 1.5 mA / cm2 membrane for 1 h ...
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bioRxiv - Biochemistry 2021Quote: Equal amount of protein was loaded and resolved on 10% polyacrylamide gel and transferred onto the PVDF membrane supplied by Bio-Rad, USA ...
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bioRxiv - Biochemistry 2021Quote: ... 80 μg of total protein for each sample was resolved by SDS-PAGE prior to transfer to nitrocellulose membranes (Bio-Rad). Membranes were blocked in Tris-buffered saline + 0.1% (v/v ...
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bioRxiv - Biochemistry 2021Quote: ... The recovery of tag-less MlaC was quantified using the ratio standard curve method (41) while that of His-tagged nanodisc-embedded complexes were quantified using the Lowry assay (DC Protein Assay, Bio-Rad). For retrograde assay ...
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bioRxiv - Neuroscience 2021Quote: ... The densities of the bands of interest were normalized to the relative expression of GAPDH or Total Protein staining by TGX stain-free pre-cast gels (4568096, Bio-Rad), SYPRO Ruby (S4942 ...
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bioRxiv - Biochemistry 2021Quote: ... Proteoliposomes or protein-free liposomes were generated by two rounds of treatment with 300 mg of wet SM-2 Bio-Beads (Bio-Rad) in total ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins (20 μg – 40 μg depending on the target protein) were resolved by 10%-12% SDS-PAGE and transferred to polyvinylidene fluoride (PVDF) membranes (BioRad, USA). The membranes were blocked with 5% non-fat milk for 2 h at RT and subsequently probed with specific primary antibodies overnight at 4°C (Table EV2) ...
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bioRxiv - Cancer Biology 2021Quote: ... the equal quantity of protein (40 µg) was resolved on 10 % polyacrylamide gel and transferred onto the PVDF membrane supplied by Bio-Rad, USA ...
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bioRxiv - Cancer Biology 2021Quote: ... MA) and 35 μg of protein were loaded and size-fractionated by SDS-polyacrylamide gel electrophoresis (Bio-Rad Laboratories, Hercules, CA). Gels were then transferred to PVDF membranes ...
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bioRxiv - Genetics 2021Quote: ... Equal amounts of proteins were resolved by polyacrylamide-SDS gel electrophoresis (PAGE) and transferred to nitrocellulose membrane (Bio-Rad, Hercules, CA) using Semi-Dry Transfer Cell (Bio-Rad) ...
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bioRxiv - Immunology 2022Quote: ... was centrifuged at 500 x g for 10 min Protein levels in the supernatant were measured by Bradford assay (Bio-Rad), and cytokine levels were measured using a custom multiplex ELISA (ThermoFisher Scientific).
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bioRxiv - Cancer Biology 2021Quote: ... The resolved proteins were transferred to Immuno-Blot® PVDF membranes using a Trans-Blot® Turbo™ Transfer System (BioRad). Membranes were incubated at 4°C overnight in primary antibodies against GRP78 ...
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bioRxiv - Pathology 2021Quote: ... and equal volumes of each suspension were loaded into two sodium dodecyl sulphate-polyacrylamide gels (SDS-PAGE; 4-20% Mini-PROTEAN TGX Protein Gels, Bio-Rad) and run at 150 V for one hour ...
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bioRxiv - Molecular Biology 2021Quote: ... Equal amounts of total protein were separated on SDS-PAGE gel by electrophoresis and transferred to a polyvinylidene difluoride (PVDF) membrane (Bio-Rad). Target proteins were detected using the following antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... Bolt 4-12 % Bis-Tris Plus Gels and Bolt MES SDS Running Buffer 20X were used for protein separation before transfer to nitrocellulose membranes (Transblot Turbo Transfer Pack, Bio-Rad) using a Transblot Turbo 0.2 μm (Bio-Rad) ...
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bioRxiv - Biochemistry 2021Quote: Protein samples were separated employing SDS-PAGE as described previously (Laemmli 1970) using a MiniProtean III electrophorese Unit (Bio-Rad, Germany) and stained with Coomassie Brilliant Blue (Thermo Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... A total of 10 μl of the collected supernatants were analyzed by SDS-PAGE and protein bands were quantified using Image Lab Software v.4.0.1 (Bio-Rad Laboratories, Inc.).
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bioRxiv - Developmental Biology 2020Quote: ... Proteins were electrophoresed on SDS-PAGE using 4-15% Mini-PROTEAN® TGX™ precast gel (Cat #456-1085, Bio-Rad), transferred onto Nitrocellulose membranes ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal masses of protein (5 ug – 40 ug) were separated by 4—15% of SDS/ PAGE and were transferred onto nitrocellulose membranes (Bio-Rad) for protein blot analysis ...
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bioRxiv - Developmental Biology 2021Quote: ... Barcoded samples were then pooled and aliquoted into individual ChIP reactions with Protein A/G magnetic beads (BioRad; 161- 4013/23) coupled with the desired antibodies (5 ug each of H3K27me3 {Millipore 07-449} ...
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bioRxiv - Cell Biology 2020Quote: ... Fluorescently tagged/labeled expressed protein was detected both before and after SDS-PAGE using a Chemidoc MP imaging system (Bio-Rad, Laboratories Pty ...
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bioRxiv - Cell Biology 2021Quote: ... cells were lysed in Native NiNTA lysis buffer (50mM NaH2PO4, 300mM NaCl, pH 8, 10mM imidazole) and protein concentration was measured via Bradford assay (Bio-Rad). 500-800μg of protein were incubated with NiNTA beads at 4°C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were loaded to each lane on a 4-20% gradient SDS/PAGE gel and transferred to nitrocellulose membranes (Bio-Rad). Signals were developed with HRP-conjugated secondary antibodies and SuperSignal West Pico Chemiluminescent Substrate (Pierce) ...
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bioRxiv - Microbiology 2021Quote: ... using a peristaltic pump and then eluted with a 0-250mM imidazole gradient using a NGC fast protein liquid chromatography (FPLC) system (Bio-Rad). The eluted samples were subject to immediate concentration with concomitant buffer exchange with fresh PBS to remove imidazole using Amicon centrifugal filters (Merck) ...
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bioRxiv - Neuroscience 2021Quote: ... Thirty micrograms of total protein from each sample were loaded on 8% SDS-PAGE and transferred to PVDF membranes (Bio-Rad).
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bioRxiv - Neuroscience 2021Quote: ... 10 µg of protein per sample were boiled for 5 min and size separated by electrophoresis in polyacrylamide gels (Bio-Rad). Proteins were transferred over-night at 4 °C to nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: The total proteins extracted from A549 cells were fractionated in a DS-Sepharose column with a BioLogic Duo-Flow system (Bio-Rad). About 40 mg of proteins in 40 ml of 10 mM phosphate buffer (pH 7.4 ...
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bioRxiv - Immunology 2021Quote: The total proteins extracted from HFL1 cells were fractionated on DS-Sepharose columns with a BioLogic Duo-Flow system (Bio-Rad). About 40 mg of proteins in 40 ml of 10 mM phosphate buffer (pH 7.4 ...
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bioRxiv - Microbiology 2021Quote: ... Proteins from lysates were separated by electrophoresis in a sodium dodecyl sulfate (SDS)-10% polyacrylamide gel (SDS-PAGE; Bio-Rad, Munich) and subsequently transferred to a nitrocellulose membrane by electroblotting ...
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bioRxiv - Immunology 2021Quote: ... Cell lysates or lung homogenates in denaturing sample buffer (10-20 µg total protein) were separated by SDS-PAGE on 4-15% Mini Protean TGX gels (Bio-Rad) and transferred electrophoretically to a 0.45 µm nitrocellulose blotting membrane (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... Aliquots of the resulting fractions were run on a 4-20% Mini-Protean TGX stain-free precast protein gel (Bio-Rad) at 200V for 30 min and stained by Imperial protein stain (ThermoFisher) ...
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bioRxiv - Immunology 2020Quote: ... 6µg of Round or Rod-shaped CCMVTT-VLPs were mixed with 2x mercaptoethanol and heated at 95°C for 3 minutes and then loaded into Any kD Mini-PROTEAN TGX precast protein gels (BIO-RAD). Gel was run for 35min at 180V ...
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bioRxiv - Immunology 2021Quote: Protein analysis was performed using Bioplex assays (27-Plex, CXCL-1-, CXCL-2-, CXCL-5-, CCL22-single plex) (Bio-Rad Laboratories) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... Cell lysate proteins were resolved on a 4–12% gradient SDS-PAGE gel and transferred to Trans-Blot nitrocellulose membrane (Bio-Rad). Blots were incubated with polyclonal goat anti-mouse TLR2 Ab (R&D Systems ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates of WT OSC were incubated with 1μg of Rabbit anti-Piwi polyclonal antibody (18) and 30 μl Surebeads Protein A Magnetic beads (Bio-Rad, 1614013). Immunoprecipitation was performed at 4°C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... 50 μl of eluted samples were electrophoresed for 8 min at 200 V on a 10% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad) and protein was stained using Quick Blue Stain ...
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bioRxiv - Cell Biology 2021Quote: ... 30 µg of protein sample was loaded onto a 4 to 15% gradient sodium dodecyl sulfate (SDS)-polyacrylamid gel (Bio-Rad), followed by protein transfer to a polyvinylidene fluoride (PVDF ...
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bioRxiv - Genetics 2020Quote: ... Collected protein samples were boiled with 1× Laemmli buffer before SDS-PAGE procedure with Mini-PROTEAN TGX Precast Gel (Bio-Rad) in Tris/Glycine Buffer (Bio-Rad) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Protein was loaded (10-50 µg per well, depending on protein of interest) and transferred using semi-dry turbo blotter (Bio-Rad). Blots were placed into 5% blocking solution containing primary antibodies and were incubated overnight at 4°C (Table 2) ...
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bioRxiv - Immunology 2021Quote: ... Tissue lysates were mixed with 2x Laemmli sample buffer and incubated at 100°C for 5 min and equal amounts of protein were loaded into a 4-15% polyacrylamide gel for SDS/PAGE and transferred onto a nitrocellulose membrane (Bio-Rad). Membranes were incubated with the following primary antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... Lysates containing approximately 40 μg of protein were boiled for 10 min prior to separation by 4-20% SDS-PAGE (BIO-RAD). After transferring at 90V to a nitrocellulose membrane for 90 min at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... Equal amounts of proteins were separated on either 10% SDS/PAGE gels or 4-20% Mini-PROTEAN TGX Stain-Free Gels (#4568094, Bio-rad). Proteins were then transferred onto PVDF membranes and probed with the following antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... adult testes or hippocampus or cell pellets were homogenized in RIPA buffer and the protein concentration was determined using a Bradford Assay kit (Bio-Rad). For blot analysis ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentration of each sample was determined as described before and a total amount of 20 μg protein/sample was resolved on 4-20% gradient gels (Bio-Rad). Proteins were transferred onto nitrocellulose or PVDF membranes futher blocked with 5% non-fat dry milk-TBST ...
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bioRxiv - Immunology 2022Quote: ... Separated proteins were transferred to a PVDF membrane using the Trans-Blot Turbo RTA Transfer Kit (Bio-Rad Laboratories, Feldkirchen, Germany) according to the manufacturer’s recommendations ...