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Citations for Bio-Rad :
8301 - 8350 of 9281 citations for Nuclear Protein Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Samples were loaded onto either a 4–20% Mini-PROTEAN® TGX Stain-Free™ Protein Gel (4568094, Bio-Rad) or 4– 20% Mini-PROTEAN® TGX™ Precast Protein Gel (4561094 ...
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bioRxiv - Biochemistry 2024Quote: Polar flagellins purified from bacterial growths of each mutant strain (2 µg) were separated by 12% SDS-PAGE as described previously [31] and stained with Coomassie Blue total protein stain (BioRad), according to the manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2024Quote: ... Protein purity and size were validated on an SDS-PAGE gel (Bio-Rad Mini-PROTEAN TGX and Mini-TETRA cell). Purified proteins were stored at –80°C.
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bioRxiv - Immunology 2024Quote: ... 70µg of protein was loaded per well for polyacrylamide gel electrophoresis using Mini-PROTEAN Precast Polyacrylamide Gels (Bio-Rad, 4561095). Gel was transferred to a low fluorescence PVDF membrane (Cytiva ...
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bioRxiv - Plant Biology 2024Quote: ... Thermo #26616) were run in pre-cast 4– 20% TGX stain-free denaturing protein gels (Biorad #4568093, #4568096, #5678094, #5678095) in a minigel system or criterion system (Biorad #1658005 ...
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bioRxiv - Immunology 2024Quote: ... Cell lysates were separated by SDS-PAGE 4 - 12 % gradient Bis-tris gel (Novex) followed by protein transfer to a PVDF membrane (BioRad) and antibody incubation ...
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bioRxiv - Cell Biology 2024Quote: ... 10 µg of protein was boiled for 5 min at 95°C and then loaded on a 4-15% SDS gel (Biorad).
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bioRxiv - Cell Biology 2024Quote: ... and the probed proteins were transferred to Bio-Rad polyvinylidene fluoride membranes using a semidry electroblotting transfer system (Bio-Rad) at 25 V for 2 h and 30 min at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were transferred from gels to the PVDF membrane using the Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad). Membranes were incubated in 5% nonfat dried milk in 1X TBST (Tris-buffered saline ...
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bioRxiv - Molecular Biology 2024Quote: ... Cell extracts were cleared at 5000g for 5min at 4°C and protein concentration was quantified by the Bradford assay (BioRad). Finally ...
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bioRxiv - Microbiology 2024Quote: ... sonicated and their protein contents were analysed by electrophoresis on TGX Stain-free pre-cast SDS-polyacrylamide gel (Bio-Rad). Proteins were then transferred on PVDF membranes (GE Healthcare ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were transferred from gels to nitrocellulose membranes for Western Blot analysis using the Turbo-Blot transfer system (Bio-Rad). Membranes were blocked using 1% w/v BLOTTO (Santa Cruz Biotechnology ...
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bioRxiv - Plant Biology 2020Quote: ... Protein concentration of the cytosolic fraction from each sample was measured using the Bradford assay (Protein Assay Dye Reagent Concentrate; Bio-Rad). After normalization ...
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bioRxiv - Microbiology 2020Quote: ... Supernatants were mixed with SDS sample buffer containing DTT and incubated for 5 min at 95°C prior to fractionation by SDS-PAGE analysis on 4%–15% Mini-PROTEAN TGX Stain-Free Protein Gels (Bio-Rad). Transfer to nitrocellulose membranes and probing for Western blot analysis was as described previously ...
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bioRxiv - Cancer Biology 2021Quote: ... The same amounts of protein extracts were subjected to polyacrylamide gel electrophoresis using the 4% to 20% Mini-Protean TGX gel system (Bio-Rad), transferred to PVDF (0.45 μm pore size ...
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bioRxiv - Evolutionary Biology 2020Quote: Samples were heated at 95°C for 10 minutes before loading into 7.5% Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad) and running at 150V for approximately 90 minutes at 4°C in 1X tris-gylcine running buffer ...
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bioRxiv - Developmental Biology 2020Quote: ... Stain-Free gels were activated with UV light for 1 minute and imaged for total protein on gel using a ChemiDoc MP imager (Bio-Rad). Proteins were transferred to PVDF blotting membranes (Bio-Rad ...
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bioRxiv - Cell Biology 2019Quote: ... Equal amounts of protein were loaded and separated by SDS-PAGE gel electrophoresis on 4%-20% Criterion gels (BioRad, Hercules, CA), and then transferred to PVDF membranes ...
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bioRxiv - Genetics 2021Quote: ... 150 μg of protein was then separated on Criterion Precast Gradient 4-15% SDS-PAGE gels (Bio-Rad, cat. number 5671083) and probed for phosphorylated EIF2α protein levels with rabbit anti-Eif2a S51 phosphorylation polyclonal antibody (1:500 dilution ...
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bioRxiv - Developmental Biology 2021Quote: ... Aliquots of larval homogenate were removed prior heat-treatment to measure soluble protein using a Bradford assay (Bio-Rad 500-0006) with BSA standard curves.
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bioRxiv - Developmental Biology 2021Quote: ... Equal amounts (30 μg) from each protein sample were separated by SDS-PAGE and transferred to PVDF membranes (PVDF; Biorad, USA). The membranes were blocked with 10% (W/V ...
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bioRxiv - Molecular Biology 2021Quote: ... Ten to twenty micrograms of denatured protein was loaded onto a 4-20% TGX stain-free polyacrylamide gel (Bio-Rad, USA) or NuPAGE 3-8% Tris acetate gel and subsequently transferred onto pre-cut nitrocellulose membranes ...
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bioRxiv - Developmental Biology 2021Quote: ... Proteins on the gels were transferred onto PVDF membrane using BioRad Mini Trans-Blot system (BioRad, Mini Trans-Blot® Cell). The membranes were washed with pure water and blocked with 5% skimmed milk in 0.1% Tween-20/PBS (PBST ...
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bioRxiv - Evolutionary Biology 2021Quote: ... SDS PAGE was performed using 7.5% or 4-20% Mini-PROTEAN® TGX™ precast protein gels (BIO-RAD, 4561023/4561094) run in running buffer (25 mM Tris ...
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bioRxiv - Developmental Biology 2020Quote: ... from Sigma was used to quantify triglyceride levels and protein levels were measured using the Quick Start™ Bradford 1X Dye Reagent (Cat. # 500-0205) from Biorad. This was followed by colorimetric estimation using TECAN Infinite M200 pro-multimode plate reader in 96-well format ...
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bioRxiv - Developmental Biology 2020Quote: ... and 50 µg of denatured proteins were run on a 12% acrylamide gel and transferred to a 0.2 µm nitrocellulose membrane (BioRad, Hercules, CA). We blocked membranes in 5% Milk in TBST (Tris Buffer Solution + 0.1% Tween ...
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bioRxiv - Cancer Biology 2022Quote: ... 15-25 μg of proteins were loaded onto 4-12% Mini-PROTEAN Tris-Tricine Precast Gels or Criterion Tris-Tricine Precast Gels (Bio-Rad), and electro-blotted on nitrocellulose membranes (GE Healthcare Life Science) ...
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bioRxiv - Systems Biology 2022Quote: ... Drugs were then added to the plates and protein was harvested after 2 hours using the Bio-Plex Pro Cell Signaling Reagent Kit (BioRad 171304006M) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins (20 μg) were separated using an Any kD gel (SDS-PAGE) and transferred to a nitrocellulose membrane (BioRad, Temse, Belgium). Aspecific binding sites were blocked for 30 min using TBS + 1% casein (BioRad ...
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bioRxiv - Neuroscience 2021Quote: 10-40 µg of denatured protein extracts were separated by SDS-PAGE using 10% acrylamide gels or 4-15% Mini-Protean precast gels (Bio-Rad) for 2 h at 120 V and transferred onto methanol-activated PVDF membranes at 350 mA for 2 h on ice ...
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bioRxiv - Molecular Biology 2021Quote: ... GFP was normalized to total protein bands per lane (visualized by a stain-free scan on a Bio-rad gel doc). Each experiment was repeated at least two times from biological replicates.
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bioRxiv - Molecular Biology 2020Quote: ... For western blotting, separated proteins were transferred onto a nitrocellulose membrane (Whatman, Dassel, Germany) using a Trans-Blot Turbo transfer system (Bio-Rad). The membrane was blocked and then incubated for 1 h with an His-tag-specific mouse mAb antibody (Proteintech ...
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bioRxiv - Molecular Biology 2020Quote: ... The proteins were electrophoresed for 70 min at 80 V in a BioRad MINI-PROTEAN Tetra system (BioRad Laboratories, CA, USA), and the gel was stained with Coomassie Brilliant Blue R-250 (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... equal amounts of protein (typically 10 μg) were separated under reducing conditions on 10 or 12 % TGX-polyacrylamide gels (Bio-Rad) and transferred to Hybond-P membrane (GE Healthcare) ...
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bioRxiv - Molecular Biology 2020Quote: ... Extracts were then incubated on ice for 10 min before protein concentration was calculated by Bradford assay (Bio-Rad, 500-0006). Extracts were then mixed with Laemmli buffer and boiled at 95°C for 5 minutes before loading on SDS-PAGE gels.
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bioRxiv - Molecular Biology 2020Quote: ... IB analysis was performed after 6-7.5% SDS-PAGE or 4-20% Mini-PROTEAN TGX Precast Protein Gels (BioRad, Hercules, CA), with overnight incubation with a 1:1000 dilution of primary antibody and followed by a 1:5000 dilution of horseradish peroxidase-conjugated anti-rabbit or anti-mouse antibody (Jackson ImmunoResearch ...
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bioRxiv - Molecular Biology 2021Quote: ... and the equivalent of 50 μg protein input (elution) were analyzed on a 4%-12% gradient XT precast Criterion gel using XT-MOPS buffer (Bio-Rad).
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bioRxiv - Molecular Biology 2020Quote: ... Proteins associated with half of the Dynabeads were eluted with 22 μL 4 χ Laemmli sample buffer (Bio-Rad, Cat.# 1610747) by boiling at 95 °C for 5 min ...
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bioRxiv - Molecular Biology 2020Quote: Following electrophoretic separation, oligonucleotide-protein complexes were transferred by electro-blotting (330 mA, 1 h) onto specific positively charged nylon membranes (Bio-Rad) and detected using an anti-digoxigenin antibody conjugated with alkaline phosphatase.
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bioRxiv - Cell Biology 2022Quote: ... were normalized by the level of total proteins in each lysate (determined using the TGX-stain free imaging technology; Bio-rad).
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... supplemented with 1 mM DTT for reduction of disulfide bonds and protein concentration was determined by Quick-start Bradford assay (Bio-Rad). Proteins were alkylated with 5.5 mM chloracetamide (45 min ...
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bioRxiv - Immunology 2021Quote: Recombinant human GFAP protein were run on 12% SDS-PAGE and transferred onto membrane via Trans-Blot Turbo Transfer System (BIO-RAD). Membrane was incubated in blocking buffer (PBS ...
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bioRxiv - Neuroscience 2019Quote: ... The denatured samples were resolved on a 4-20% Mini-PROTEAN TGX Stain-Free precast protein gel (Bio-Rad, cat# 4568094) and transferred to a nitrocellulose membrane (0.45 μm ...
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bioRxiv - Molecular Biology 2019Quote: ... Beads were washed four times with binding buffer containing 0.1% Tween-20 and bound proteins were eluted with 2X Laemmli Sample Buffer (Bio-Rad Laboratories). Eluted proteins were subjected to electrophoresis on a 17% resolving gel at 120V for 2 hrs and transferred to PVDF membrane at 180mA for 2 hrs and immunoblotted with His-Probe-HRP (Pierce ...
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bioRxiv - Genetics 2019Quote: ... membranes were washed three times for five minutes each in TBST and protein detected using Clarity western ECL substrate (Bio-Rad) according to the manufacturer’s instructions.
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bioRxiv - Physiology 2019Quote: ... protein samples (20 µg of total protein) were separated at 300 V for 30 min using 4-20% gels (Criterion TGX, Bio-Rad), followed by wet transfer to PVDF membranes (0.2 µm Immun-Blot ...
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bioRxiv - Neuroscience 2019Quote: ... Equal amounts of protein per sample (5 or 10ug) was loaded into a 4-20% gradient precast mini-PROTEAN TGX gel (Bio-Rad) and proteins were separated with 200V in 1x running buffer (24.76 mM Tris base ...
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bioRxiv - Physiology 2020Quote: ... equal amounts (30 μg) of protein were resolved on 4–20% TGX stain-free gels (Bio-Rad Laboratories GmbH, Munich, Germany) and transferred onto PVDF membranes ...
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bioRxiv - Molecular Biology 2019Quote: ... 5-15 µg of protein was resolved by SDS/PAGE and transferred to nitrocellulose membranes using the Trans-Blot Turbo System (Bio-Rad). Membranes were blocked in 5% milk in phosphate buffered saline with 0.1% Tween (PBS-T ...
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bioRxiv - Immunology 2019Quote: ... The lysates were transferred to a new Eppendorf tube and total protein in each sample was determined using Lowry assay (Bio-Rad). Equal protein (20ug per lane ...