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Citations for Bio-Rad :
7751 - 7800 of 9281 citations for Nuclear Protein Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... stain-free gels were imaged following UV exposure to visualise total protein content (ChemiDoc MP Imaging System, Bio-Rad). Alternatively ...
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bioRxiv - Immunology 2023Quote: ... and Tris-Glycine SDS buffer (Geneflow B9-0032) or 4–12% Criterion™ XT Bis-Tris Protein Gels (BioRad) and XT MES running buffer (BioRad 1610789) ...
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bioRxiv - Cancer Biology 2023Quote: ... clarified by centrifugation at 13.2K rpm and protein concentration was determined by BCA assay (Bio-Rad, Hercules, California, USA). For western blot analysis ...
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bioRxiv - Immunology 2023Quote: ... Proteins were then electro-transferred onto PVDF (polyvinylidene fluoride) membranes with Trans-Blot Turbo transfer system (Bio-Rad Laboratories). Antibodies used for the detection are listed in Table S1 ...
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bioRxiv - Genetics 2023Quote: ... and the protein bands were detected using the chemiluminescent Clarity Max™ Western ECL Substrate (Bio-Rad, Cat# 1705062S). The bands were visualized in the ChemiDoc Imaging System (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 μg of protein was loaded on a Mini-PROTEAN TGX gel 4-20% gradient pre-cast gels (BioRad). Protein was transferred to 0.45 μm nitrocellulose membrane (Biorad ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were resolved by SDS-polyacrylamide gel electrophoresis using 4-15% Mini-PROTEAN® TGX™ gels (BioRad; #4561086) and transferred to nitrocellulose membranes (BioRad ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were separated using SDS-PAGE and transferred to nitrocellulose membrane using Trans-Blot Turbo transfer system (Bio-Rad). Membranes were blocked in 5% skimmed milk diluted in Tris-buffered saline with 0.1% Tween-20 (TBS-T ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant containing the protein was filtered (0.45 µm) and applied on an IMAC Ni2+-NTA column (Bio-Rad) for FPLC purification ...
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bioRxiv - Biophysics 2023Quote: ... the Ni-NTA purified protein was buffer exchanged into the storage buffer over a 10-DG column (Bio-Rad) and was stored at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... 15µg of protein lysate was loaded into a 4-20% polyacrylamide Criterion TGX stain-free gel (Bio-Rad, #5678095). Electrophoresis was performed using 60V for one hour followed by 125V until finished ...
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TNFR1/p38αMAPK signaling in Nex+ supraspinal neurons regulates sex-specific chronic neuropathic painbioRxiv - Neuroscience 2023Quote: Protein extracts were run on sodium dodecyl sulphate (SDS)-polyacrylamide gels and transferred to nitrocellulose membranes (Turbo blot, Biorad). Membranes were blocked for 2 hours in 5% non-fat milk (BioRad ...
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bioRxiv - Molecular Biology 2023Quote: ... His-tagged recombinant protein was purified using Ni-NTA purification with the help of Ni-charged resins (Bio-Rad). Purified protein was further used for kinase assay ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were separated by gel electrophoresis in 12% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad, 4561043) and transferred to membranes ...
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Functional characterization of ATP13A2 variants associated with distinct neurodegenerative disordersbioRxiv - Molecular Biology 2023Quote: ... 10 μg of protein was loaded on a NuPage 4-12% Bis-Tris gel (Thermo Fisher Scientific, Bio-Rad) and separated during an electrophoresis run at 150 V in MES running buffer (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... 25 µl of non-boiled protein sample were loaded on 12% Mini-Protean TGX pre-cast gels (Bio-Rad) and the proteins separated by SDS-PAGE ...
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bioRxiv - Immunology 2023Quote: ... 0.5ug for the IgA blot and 5ug for the SC blot of LCTG-002 was loaded on a 7.5% pre-casted Gel Mini Protein gel TGX (BioRad) in native gel buffer ...
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bioRxiv - Cell Biology 2023Quote: Samples were prepared mixing 20μg of protein from each sample with 6.25μL of Laemmli Buffer 4X (Biorad, Hercules, USA), 1.25μL β-mercaptoethanol (BME ...
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bioRxiv - Plant Biology 2023Quote: ... Leaf lysates were cleared by centrifugation at 10,000×g for 5 min and total protein normalized via Bradford Assay (Biorad). Extracts were prepared for SDS-PAGE with a 5x loading buffer containing 10% (w/v ...
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bioRxiv - Molecular Biology 2023Quote: ... the concentration was calculated by the Bradford assay using a Quick Start Bradford Protein Assay Kit 2 (Bio-Rad).
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bioRxiv - Microbiology 2023Quote: ... The sample was pre-cleared via incu-bation with 30 µl of SureBeads Protein A magnetic agarose beads (BioRad) for 30 min at RT ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were separated on the 10% SDS-PAGE gel (Hangzhou Fudebio) and transferred onto the PVDF membranes (Bio-rad). Membranes were blocked in the blocking buffer (Beyotime Biotechnology) ...
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bioRxiv - Biochemistry 2023Quote: ... The transfer of protein bands to a polyvinylidene fluoride membrane was performed using the Transblot Turbo system (Bio-Rad Laboratories Pty Ltd. ...
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bioRxiv - Biochemistry 2022Quote: ... proteins were electrophoretically transferred onto a polyvinylidene fluoride (PVDF) membrane using Semidry Trans-Blot apparatus (Bio-Rad, Hercules, CA) at 20 V for 20 min ...
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bioRxiv - Immunology 2023Quote: ... The protein bands were visualized using an ECL kit (Meilunbio, CHN) and imaged on ChemiDoc XRS+ (Bio-Rad, USA).
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bioRxiv - Cancer Biology 2023Quote: ... 40 μg of total cellular proteins were loaded to the gels after denatured in 4× Laemmli buffer (Bio-Rad) at 37 °C for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... To visualize the protein bands, membranes were incubated (5 min, RT) in Clarity™ western ECL substrate (Bio-Rad) and imaged using the ChemiDoc™ MP imaging system (Bio-Rad) ...
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bioRxiv - Genetics 2023Quote: ... Proteins were blotted onto ready-to-use Trans-Blot Turbo transfer pack using the Trans-Blot Turbo system (BioRad) at 2.5 A (25 V ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins (25 μg/lane) were separated on SDS-polyacrylamide gels and transferred to polyvinylidene difluoride membranes (Bio-Rad Laboratories). For the detection of α- syn ...
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bioRxiv - Immunology 2023Quote: The biotinylated ODN-bound proteins were precipitated with streptavidin beads from mitochondria lysates and resolved by PAGE (Bio-rad). The PAGE gels were stained using a silver staining kit (Pierce) ...
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bioRxiv - Genetics 2022Quote: ... Samples were resolved by SDS polyacrylamide gel electrophoresis on 4%-15% CriterionTM TGX Stain-FreeTM Protein Gel (BioRad #5678083) using 30μg of whole cell lysate ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples at a standard protein concentration were diluted in Western blot sample buffer consisting of Laemmli buffer (Bio-Rad) and 5% ý-mercaptoethanol (Merck) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell lysates (50 µg) were loaded into 4–20% PROTEAN® TGX™ Precast Protein Gels (BioRad, Cat# 5678081). After overnight transfer to nitrocellulose membranes ...
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bioRxiv - Cell Biology 2023Quote: ... 700 µg of each sample were incubated with 30 µL of slurry Affi-Prep protein A resin (Bio-Rad) and 1 µg of mouse anti-V5 tag antibody (Invitrogen ...
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bioRxiv - Plant Biology 2023Quote: ... Ten µl of the supernatant were used to determine protein concentrations by the dye-binding Bradford assay (Bio-Rad). Twenty-five µg of total proteins in a final volume of 100 µl were pipetted into a white 96 well plate (Greiner Bio-One ...
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bioRxiv - Biochemistry 2023Quote: Protein lysates were boiled in reducing sample buffer and resolved on 4-20% Criterion TGX polyacrylamide gels (Bio-Rad), then transferred to 0.2 µm nitrocellulose membranes (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... then separated from the protein by boiling for 10 mins at 100℃ with 4x Laemmli Sample Buffer (BioRad, #1610747).
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bioRxiv - Cell Biology 2023Quote: ... Migrated proteins were transferred from the gels to PVDF membranes using the Trans-Blot Turbo Transfer System (Bio-Rad) and run settings of 2.5A ...
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bioRxiv - Cell Biology 2023Quote: ... 1mg cell lysate or 500 µg cytosolic or nuclear fraction were incubated with 2μg antibody or normal IgG and pulled down by 5µL protein G-magnetic beads (Bio-Rad). Samples were then pulled down by protein-G-magnetic beads and subjected to WB or proteomics analysis ...
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bioRxiv - Neuroscience 2023Quote: ... were centrifuged at 20,000 x g for 25 min at 4⁰C and supernatants were assayed for total protein (Bradford assay, BioRad). Aliquots of WCL (30 µg total protein ...
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bioRxiv - Plant Biology 2023Quote: ... 20 µL samples were separated by 4-15% SDS-PAGE (Mini-PROTEAN® TGX™ Precast Protein Gels, BioRad). A mixture of recombinant proteins was used as a standard ...
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bioRxiv - Physiology 2023Quote: ... Total protein (5∼15 µg) was loaded and separated by 4–20% pre-made SDS-PAGE gels (#4568095, BioRad, Mini-PROTEAN TGX Stain-Free Precast Gels ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins (25 μg/lane) were separated on SDS-polyacrylamide gels and transferred to polyvinylidene difluoride membranes (Bio-Rad Laboratories). Membranes were immunoblotted for CSF1R (1:250 ...
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bioRxiv - Physiology 2023Quote: ... Equal amounts of protein were fractionated by SDS-PAGE (Mini-PROTEAN TGX Precast 4– 15% gradient gels, Bio-Rad), and eletro-transferred onto nitrocellulose membrane (Trans-Blot Turbo Transfer System ...
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bioRxiv - Neuroscience 2023Quote: ... Protein content of supernatant was quantified via Bradford assay according to the manufacturer’s instructions (catalogue # 500-0006, Bio-Rad). Absorbance was measured at 595nm using a Pherastar FS (BMG LABTECH).
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bioRxiv - Neuroscience 2023Quote: ... Protein extracts were denatured 5 min at 95°C in a Laemmli buffer (Bio-Rad, Marnes-la-Coquette, France) with β-mercaptoethanol (1/10 ...
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bioRxiv - Immunology 2024Quote: ... The indicated mass of protein was boiled for 5 minutes at 95°C with 4x Laemmeli Buffer (BioRad #1610747) supplemented with 2.5% (v/v ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were quantified by Pierce Reducing Agent Compatible BCA Protein Assay and run on 4-20% TGX gels (Biorad). Membranes were probed with anti-FLAG M2 antibody (Sigma ...
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bioRxiv - Cell Biology 2024Quote: Protein extracts dissolved in Lämmli sample buffer or SDS sample buffer were separated by SDS-PAGE (Biorad Mini Protean) and transferred to ethanol activated Amersham Hybond-P 0.45 µm PVDF membranes (Cytiva ...
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bioRxiv - Cell Biology 2024Quote: ... proteins were resolved by 8.5% SDS-PAGE gel electrophoresis and transferred to PVDF membranes (Bio-Rad, Mississauga, ON, Canada). Membranes were blocked with 5% non-fat milk powder in Tris-buffered saline- Tween (TBS-T ...