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Citations for Bio-Rad :
7601 - 7650 of 9281 citations for Nuclear Protein Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Proteins were transferred from the remainder of the gel to nitrocellulose membrane using the Trans-Blot Turbo (Bio-Rad). Membranes were incubated in a 5% milk in 1X Tris-buffered saline ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins where eluted from the beads by directly adding 20 µL of Laemli Sample Buffer buffer 2X (Biorad, #1610747). Samples where then processed for SDS-PAGE and Western blotting.
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bioRxiv - Biochemistry 2020Quote: ... Protein bands were quantified on a BioRad Chemidoc Touch Imaging System using Image Lab software version 6.0.1 (Bio-Rad).
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bioRxiv - Cell Biology 2019Quote: ... Data were normalized to GAPDH expression or total protein determined via UV treatment of TGX Stain-Free gels (Biorad) followed by detection on the transfer membrane.
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bioRxiv - Developmental Biology 2020Quote: ... Proteins were separated on a SDS-PAGE Mini-PROTEAN TGX 4-15% gradient gel (Bio-Rad, catalog #456-1086) along a 1:1 mix of Amersham ECL Rainbow Molecular Weight Markers (Amersham # 95040-114 ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were denatured and proteins separated in SDS-PAGE in Criterion™ TGX Stain-Free™ Gels (Bio-Rad), and transferred onto nitrocellulose membranes with the Trans-Blot® Turbo™ Blotting System (Bio-Rad).
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bioRxiv - Biochemistry 2021Quote: ... The protein was incubated at 4°C for 1 h with Bio-Beads™ SM-2 resin (Bio-Rad) equilibrated with 100 mM Tris-HCl ...
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bioRxiv - Neuroscience 2020Quote: ... Fifteen μg of protein per sample were separated in 4-20% SDS-polyacrylamide gel (BIO-RAD mini-protean-TGX) before electrophoretic transfer onto a nitrocellulose blotting membrane (Amersham ...
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bioRxiv - Microbiology 2021Quote: ... the remaining interphase was dried completely before resuspending in Tris-buffered saline (50mM Tris-Cl, 150mM NaCl, pH 7.6) for protein quantification using a Bradford Assay (BioRad). Aqueous phase was evaporated under nitrogen flow and used for LC-MS ...
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Transcriptome analyses reveal tau isoform-driven changes in transposable element and gene expressionbioRxiv - Neuroscience 2021Quote: ... 12.1 ug of protein from each sample was loaded onto a 10% Tris-Glycine gel (Biorad, cat no. 4561034) and then transferred onto a PVDF membrane and blocked for one hour with 5% BSA in 0.1% Tween/1xTBS ...
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bioRxiv - Neuroscience 2021Quote: ... Both the soluble (40 µg of proteins) and the insoluble (4 µl) fractions were spotted on nitrocellulose membrane (BioRad). The near infrared fluorescence signal was acquired by scanning the membrane at the LiCor Odissey using the 800 nm filter and images were analyzed using the Fiji software.
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bioRxiv - Microbiology 2020Quote: ... and the images were analyzed and the signals quantitated after total cell protein normalization using the Image Lab 6.0.1 software (Bio-Rad).
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bioRxiv - Microbiology 2020Quote: ... and 150 ml Protein A Sepharose (GE, 17-5138-01) in a Poly-Prep Chromatography Column (BioRad, 731-1550). Fabs were generated by Papain cleavage and purified by the use of the Pierce Fab Preparation Kit according to the manufacturers’ instructions (Thermo Scientific - #44985) ...
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bioRxiv - Cell Biology 2020Quote: ... SDS-PAGE was made with 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels 10-well (BioRad, 4561094) with 30 μg of protein lysate for well and running was 70 V for 2 hours ...
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bioRxiv - Microbiology 2020Quote: ... and 30μg of total protein per sample was mixed with Laemmli buffer supplemented with 2-mercaptoethanol (10%) (Bio-rad) and boiled at 99°C for 10 minutes ...
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bioRxiv - Bioengineering 2021Quote: ... A sample containing 1-20µg of protein was loaded into a 4-15% precast gel (Bio-Rad Mini-ProTEAN) and run at 60V for 20min followed by 160V for 1 hour ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were electrophoresed on SDS-PAGE using 4-15% Mini-PROTEAN® TGX™ precast gel (Biorad#456-1085) and transferred onto a nitrocellulose membrane using Trans-Blot® Turbo™ Transfer Pack (Biorad#170-4159) ...
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bioRxiv - Molecular Biology 2020Quote: ... The immunoprecipitated proteins were eluted by heating at 100°C during 15 minutes in 4x Laemmli Sample Buffer (BIORAD). Immunoprecipitated protein complexes were subjected to Western blot analysis ...
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bioRxiv - Immunology 2020Quote: ... Protein (15–100 μg per lane) was separated in polyacrylamide gels containing SDS and transferred to Nitrocellulose membrane (Biorad) and probed with different primary antibodies according to the manufacturer’s protocols ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins (50 μg) were resolved on 12.5 % SDS-PAGE gels and via electrophoretic transfer system (Bio-Rad, München, Germany) bonds moved to polyvinylidene fluoride (PVDF ...
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bioRxiv - Biophysics 2021Quote: ... coli HU protein was monitored using 20% SDS-PAGE and the concentration was determined using the Lorry assay (Biorad). The purified E ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were separated by SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis) and transferred onto nitrocellulose membrane (Bio-Rad) using TransBlot Turbo (Bio-Rad ...
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bioRxiv - Genetics 2022Quote: ... Eight micrograms of total protein lysate were resolved on 4–15% Mini-PROTEAN TGX Stain-free gels (Bio-Rad) and transferred onto Immuno-Blot PVDF membranes (Bio-Rad) ...
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bioRxiv - Genetics 2022Quote: ... The separated protein samples were transferred to the PVDF membranes using a Mini Trans-Blot Electrophoretic Transfer Cell (Biorad) at 100V for 1h ...
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bioRxiv - Pathology 2022Quote: ... The expression levels of target proteins were quantified with Quantity One 1-D Analysis Software (Bio-Rad, Hercules, CA) and normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Systems Biology 2022Quote: Samples with ∼30 μg of soluble total proteins were loaded on 4%-20% TGX stain-free precast gels (Biorad). These proteins were then separated by electrophoresis and transferred on PVDF membrane using TurboBlot (Biorad) ...
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bioRxiv - Immunology 2022Quote: ... 30 ug of Protein extracts were separated by 4-12% Bold NuPage (ThermoScientific) and transferred onto PVDF membranes (BioRad). After blocking with 3% albumin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... Equal amounts of protein were separated by SDS-PAGE and transferred to 0.2 μm nitrocellulose membranes (BioRad, Cat# 1620112). After blocking the membranes in 2% BSA in Tris buffered saline (TBS)-Tween for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Twenty (HA blot) or forty (SREBP1 blot) micrograms of protein were mixed with Laemmli sample loading buffer (Bio-Rad), heated to 37 °C for 10 min ...
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bioRxiv - Biochemistry 2022Quote: ... Protein-associated fluorescence intensities (arbitrary units, AU) were analyzed using Quantity One image analysis software (BioRad, Hercules, CA, USA). Gels were stained with Colloidal Coomassie Brilliant Blue G250 and scanned.
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bioRxiv - Cancer Biology 2022Quote: ... Approximately 10μg of protein was loaded per sample on a 4-15% SDS-PAGE Stain-Free TGX precast gel (BioRad). Gels were run at 150V for approximately 60 minutes ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein samples were separated on the 4-15% Mini-PROTEAN® TGX™ precast SDS-PAGE gels (Bio-Rad) and then transferred to polyvinyl difluoride (PVDF ...
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bioRxiv - Microbiology 2019Quote: ... Protein lysates were separated in 10% SDS-PAGE gel (Transgen, Beijing, China) by Mini-PROTEAN tetra Cell system (BioRad) and transferred to PVDF membrane ...
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bioRxiv - Genetics 2020Quote: ... Whole cell lysate protein samples (20-50 µg) were resolved on Criterion 4-20% gradient denaturing gels (Bio-Rad), transferred to nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Plant Biology 2020Quote: ... This activity was then standardized to the quantity of total proteins present in the sample (Bradford assay, Bio-Rad). For inhibitor assays ...
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bioRxiv - Physiology 2020Quote: ... Twenty micrograms of protein were separated by 4-15% Mini-PROTEAN TGX Precast Gels (PAGE) (Bio-Rad, Hertfordshire, UK) and transferred to a nitrocellulose membrane ...
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bioRxiv - Microbiology 2019Quote: ... β-galactosidase activity units were normalized to total proteins per ml as determined using Bradford assay reagents (Bio-Rad). Assays were performed in biological triplicates.
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bioRxiv - Biochemistry 2019Quote: ... Proteins in whole-cell lysates were separated on 4-15 % Mini-Protean TGX Precast gels (456-1085, Bio-Rad) and transferred to 0.2 μm Nitrocellulose membranes (1704158 ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates containing 30 μg protein were subjected to SDS-PAGE using 10% Criterion TGX Stain-Free gels (BioRad, UK) on a mini-Protean Tetra system (Bio-Rad ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and 15μg protein in 5x Laemmli buffer with 5% b-mercaptoethanol was loaded on Mini-PROTEAN TGX Precast Gels (BioRad). Gel lanes were cut into three sections for peptide extraction ...
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bioRxiv - Molecular Biology 2020Quote: ... equal amounts of total protein were loaded onto 4–20% SDS-PAGE gels (Mini-PROTEAN TGX Gel, Bio-Rad). After SDS-PAGE ...
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bioRxiv - Neuroscience 2021Quote: ... Equivalent amounts of protein were subjected to electrophoresis on a 4-12% Mini-PROTEAN® TGXTM Gel (Bio-Rad) and transferred to a Hybond-ECL nitrocellulose membrane (Amersham - GE Healthcare Life Sciences) ...
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bioRxiv - Plant Biology 2020Quote: ... Protein concentrations were determined using the Bio-Rad Assay according to the manufacturer’s instructions (Bio-Rad Laboratories, Munich, Germany) based on the method of (48).
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bioRxiv - Biochemistry 2020Quote: ... Protein samples were then separated on 12 % polyacrylamide gels (Mini-Protean II electrophoresis cell, BioRad, Marnes-la-Coquette, France) and transferred to 0.2 μm nitrocellulose blotting membrane (GE Healthcare ...
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bioRxiv - Synthetic Biology 2020Quote: ... The resolved proteins were then transblotted to nitrocellulose membranes using the Trans-Blot Turbo Transfer System from Bio-RAD. The membrane was blocked with 5% skim milk in TBST and probed with HRP-conjugated anti-flag mouse monoclonal antibody (Thermofisher ...
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bioRxiv - Microbiology 2021Quote: ... Protein samples (20 µg) were separated by SDS-PAGE (Mini-protean TGX any-kD, Bio-Rad, Hercules, CA, USA) and transferred onto a PVDF membrane (0.2 µm ...
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bioRxiv - Microbiology 2020Quote: ... and analyzed by SDS-PAGE on 10% Mini-PROTEAN® TGX™ Precast Protein Gels (Biorad, Hercules, CA, USA). Gels were fixed in 1 M sodium salicylate for 30 min and dried ...
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bioRxiv - Microbiology 2020Quote: One ug of each respective protein was mixed at a 1:1 ratio with 2X Laemmli buffer (Bio-Rad) which was supplemented with 2% β-mercaptoethanol (Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2020Quote: ... 30μl of protein sample was run on SDS-PAGE (protean TGX pre-casted gradient gel, 4%-20%, Bio-rad) and transferred to the Nitro-Cellulose membrane (Bio-Rad ...
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bioRxiv - Developmental Biology 2021Quote: ... Proteins were transferred to methanol-treated PVDF membranes using a Mini Trans-Blot Cell wet-transfer apparatus (BIO-RAD), according to manufacturer’s specifications at 400 mA for 1.5 hours ...