Labshake search
Citations for Bio-Rad :
7651 - 7700 of 9281 citations for Nuclear Protein Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2021Quote: ... Samples were separated by SDS-PAGE electrophoresis using 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad) followed by detection of ubiquitinated substrate by immunoblotting using anti-MBP (New England Biolabs) ...
-
bioRxiv - Genomics 2019Quote: ... All blots were visualized using a goat anti-rabbit IgG:alkaline phosphatase fusion protein at 1:3,000 dilution in conjunction with BCIP/NBT color development (Bio-Rad).
-
bioRxiv - Biochemistry 2019Quote: Matrix Gla protein microsome suspensions were also analyzed using Novex gels followed by transfer to a 0.2 micron nitrocellulose membrane (BioRad). Membranes were analyzed using an antiFLAG antibody (Sigma ...
-
bioRxiv - Microbiology 2019Quote: ... 10 μL of each sample was separated by SDS-PAGE in 4–20% Mini-Protein TGX gels (Bio-Rad) and stained with Coomassie dye for total protein ...
-
bioRxiv - Genomics 2021Quote: The proteins were separated electrophoretically using a 4–12% gradient gel and transferred to a nitrocellulose membrane (Bio-Rad, Trans-Blot® Transfer Medium ...
-
bioRxiv - Cell Biology 2020Quote: ... Protein was transferred to a PVDF membrane using a Trans-Blot Turbo transfer system (Bio-Rad, Hercules, CA, USA), according to the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2020Quote: Proteins were resolved on 4-20% polyacrylamide gradient Tris-glycine gels SDS-PAGE gels (Biorad, 567-1095, 456-1096) and transferred onto 0.2 µm pore size PVDF membranes (Biorad ...
-
bioRxiv - Physiology 2021Quote: ... A total of 30 μg of protein was separated by SDS-PAGE and transferred to nitrocellulose membranes (Bio-Rad) using the semidry Trans-Blot Turbo device (Bio-Rad) ...
-
bioRxiv - Molecular Biology 2021Quote: ... 35 μg of protein was resolved on 4-12% polyacrylamide gradient Criterion XT Bis-Tris Precast Gels (Biorad Laboratories) and transferred to a nitrocellulose membrane (Sigma) ...
-
bioRxiv - Neuroscience 2021Quote: ... Radiolabel incorporation was measured with a scintillation counter and samples were subjected to a protein concentration assay (Bio-Rad). Data was analyzed as counts per minute per microgram of protein ...
-
bioRxiv - Cancer Biology 2021Quote: ... 80 μg of total protein was separated by SDS-PAGE on a 4-15% Criterion TGX Gel (Bio-Rad) and transferred to a nitrocellulose membrane using the Trans-Blot Turbo system (Bio-Rad) ...
-
bioRxiv - Biochemistry 2021Quote: ... the membranes were washed several times and proteins were visualized using the Clarity™ Western ECL Substrate (Bio-Rad) and the ChemiDoc Imaging System (Bio-Rad).
-
bioRxiv - Biochemistry 2021Quote: ... 5 μM of proteins and 3x of SYPRO™ Orange were pipetted into a 96-well PCR plate (BioRad, MicroAmp Fast 96-Well Reaction plate ...
-
bioRxiv - Biochemistry 2021Quote: ... The enriched protein were eluted by heating at 95°C for 30 minutes in 1X Laemmli sample buffer (BioRad) and processed to western blot ...
-
bioRxiv - Synthetic Biology 2021Quote: ... following the manufacturer’s instruction (4–15% Mini-PROTEAN® TGX Stain-Free™ Protein Gels, 4568084, BioRad, Hercules, CA) with the gel apparatus (Mini-PROTEAN Tetra Cell ...
-
bioRxiv - Neuroscience 2021Quote: ... Immunoblotting was performed using standard protocols with 10μg of protein loaded onto SDS-PAGE pre-cast gels (Bio-Rad). Antibodies used were anti-DDX3X (rabbit polyclonal ...
-
bioRxiv - Neuroscience 2022Quote: ... Equal amounts of protein were loaded onto 10% or 12% pre-cast Tris-HCl Mini-PROTEAN gels (Bio-Rad) and proteins separated by electrophoresis ...
-
bioRxiv - Biochemistry 2021Quote: ... The supernatant was then transferred into a new tube and protein concentration was determined by BCA assay (Bio-Rad). Immunoblotting was performed using CDCP1(D1W9N ...
-
bioRxiv - Cell Biology 2021Quote: ... Proteins were separated by SDS-PAGE in 12% Bis-Tris gels and transferred onto 0.45 µm nitrocellulose membranes (BioRad). The blots were incubated overnight at 4°C with anti-αSyn (1:100 ...
-
bioRxiv - Cell Biology 2020Quote: ... proteins were separated by SDS-PAGE and transferred to nitrocellulose using a Trans-blot Turbo system (Bio-Rad Laboratories). Species-specific secondary antibodies conjugated to horseradish peroxidase (HRP ...
-
bioRxiv - Genomics 2020Quote: ... The proteins were transferred onto PVDF membranes using the Mini Trans-Blot Electrophoretic Transfer Cell (Bio-Rad, 170-3930) at 80 V ...
-
bioRxiv - Developmental Biology 2021Quote: ... Samples were subsequently aliquoted by mixing 75μl of protein extract with 25μl of 4X Laemmli buffer (Bio-Rad #1610747) and stored at -20°C ...
-
bioRxiv - Microbiology 2020Quote: ... 25 µg of protein was run per sample on 4-15% Mini-PROTEAN TGX stain-free precast gels (BioRad) at 150V ...
-
bioRxiv - Immunology 2021Quote: ... Protein extracts were separated by SDS-PAGE with a 10% gel and transferred to nitrocellulose membranes (Bio-Rad Laboratories). The membranes were probed with anti-spike Ab (40150-T62 ...
-
bioRxiv - Immunology 2021Quote: ... 40ug of protein was loaded per well for polyacrylamide gel electrophoresis using Mini-PROTEAN Precast Polyacrylamide Gels (Bio-Rad). Western blotting was performed using low fluorescence PVDF membrane (Bio-Rad) ...
-
bioRxiv - Immunology 2020Quote: ... For the visualization the proteins ECL-solution (Luminol, Para-Hydroxycoumarinacid, DMSO) and ChemiDoc Touch™ Imaging System (BIO-RAD) was used ...
-
bioRxiv - Immunology 2020Quote: ... Protein for immunoblotting was eluted from 25 percent of beads by boiling in 2X Laemmli sample buffer (Bio-Rad). RNA was extracted from 75 percent of beads using TRIzol reagent (Thermo Fisher Scientific) ...
-
bioRxiv - Cell Biology 2021Quote: ... Soluble proteins were separated using SDS-PAGE and transferred to a 0.45 µm nitrocellulose membrane (Cat# 1620115, Bio-Rad). Primary antibodies (Table 2 ...
-
bioRxiv - Developmental Biology 2020Quote: ... Lysate was spun down at 14000 rpm and protein concentration was determined using the Bradford assay as detailed in the manufacturer’s kit (Biorad). Samples were denatured with SDS and methylene blue ...
-
bioRxiv - Cell Biology 2021Quote: ... Proteins from a second identically loaded gel were transferred to PVDF membrane using a semi-dry transfer method (BIORAD) and a western blot was performed using anti-HIS (Sigma H-1029 ...
-
bioRxiv - Molecular Biology 2021Quote: ... A total of 20ug of protein per sample was resolved using 4%-15% TGX stain-free gels (Bio-Rad), followed by immunoblotting using PCF11 (Proteintech ...
-
bioRxiv - Neuroscience 2020Quote: ... Lysates were cleared of debris by centrifugation at 15,000 x g for 15 min at 4°C and then normalized for protein content using Bradford assay (BioRad). Normalized protein lysates were fractionated by 10 % SDS/PAGE and transferred onto a PVDF membrane (GE Healthcare Life Sciences ...
-
bioRxiv - Neuroscience 2022Quote: ... Proteins were transferred to 0.2 μm Nitrocellulose membranes using semi-dry transfer (Bio-Rad Transblot Turbo; 25V 30 minutes). Membranes were blocked using 5% milk in Tris-buffered saline supplemented with 0.1% Tween-20 (TBST ...
-
bioRxiv - Immunology 2022Quote: ... Lysates from 3.107 to 4.107 of cells were subjected to immunoprecipitation according to manufacturer’s protocol for the Sure Beads Protein G Magnetic Beads (161-4023, Biorad) using a rat anti-FLAG antibody (Biolegend) ...
-
bioRxiv - Microbiology 2022Quote: ... and 10-15 µg of protein was loaded into 12% acrylamide Criterion XT Bis-Tris Precast Gels (Bio-Rad). Electrophoresis was performed with NuPage MES SDS Running Buffer (Invitrogen ...
-
bioRxiv - Cancer Biology 2022Quote: ... and protein levels were quantified using BCA Assay (Pierce) and supplemented with 4x Laemmli Buffer with 2-mercaptoethanol (Biorad). Samples were boiled and sonicated ...
-
bioRxiv - Cancer Biology 2022Quote: ... The resolved proteins were transferred onto a polyvinylidene difluoride membrane using the Trans-Blot Turbo Transfer System (Bio-Rad). The membranes were stained using 5% Ponceau S solution ...
-
bioRxiv - Biochemistry 2022Quote: ... and protein purity was determined by SDS-PAGE analysis (Figure S8) and densitometry on Image Lab 6.0 (Bio-Rad).
-
bioRxiv - Cancer Biology 2022Quote: ... 50µg of protein were loaded and electrophoresed through a 4-15% Mini-PROTEAN TGX precast gel (BIO-RAD, 4561084). Proteins were transferred onto a PVDF membrane ...
-
SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... proteins were separated on a 4-15% Mini-PROTEAN TGX Stain-Free SDS-PAGE gel (#456-8084; Bio-Rad) and transferred to a 0.2 μm PVDF membrane (#ISEQ00010 ...
-
bioRxiv - Immunology 2022Quote: ... Proteins were separated on a 4-15% sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) precast gel (Bio-Rad) at room temperature ...
-
bioRxiv - Plant Biology 2022Quote: ... 4 °C) and the supernatant was taken up and the protein concentration was determined using the Bradford assay (Biorad).
-
bioRxiv - Physiology 2022Quote: ... S-nitrosylated proteins were separated using 10% SDS-PAGE and transferred onto a PVDF membrane (BioRad, Hercules, CA, USA). A monoclonal anti-Cx43 antibody (Sigma ...
-
bioRxiv - Molecular Biology 2022Quote: ... The protein concentrations of culture medium and cell lysate were determined by the Bradford assay (Bio-Rad, Hercules, CA). Culture medium or cell lysate samples were resuspended in SDS sample buffer containing β-mercaptoethanol and incubated at 95°C for 5 min ...
-
bioRxiv - Molecular Biology 2022Quote: ... 10 µg of protein extract were loaded onto a Mini-PROTEAN TGX Precast Gel (4-15% gradient, BioRad, 4561086) in 1xLaemmli buffer (BioRad ...
-
bioRxiv - Neuroscience 2022Quote: ... Samples (50µg of protein/lane) were separated on a 4-12% SDS-polyacrylamide gel (Bio-Rad, Hercules, CA, USA) and probed with anti-ACK antibody (1:100 ...
-
bioRxiv - Microbiology 2022Quote: ... total protein was visualised post-transfer to PVDF membranes on ChemiDoc using Stain-free fluorescent dye (Bio-Rad Laboratories). For analyses of protein band intensities ...
-
bioRxiv - Immunology 2022Quote: ... loaded onto a 12% acrylamide gel and separated proteins transferred onto a PVDF membrane (Bio-Rad, Hercules, California, US). Membranes were washed in 1X TBS ...
-
bioRxiv - Microbiology 2022Quote: ... the denaturalized proteins were separated in a 12% SDS-PAGE precast gel (Mini-PROTEAN TGX Precast Gels, Bio-Rad). Electrophoresis was run at 250 V (PowerPac Basic power supply ...
-
bioRxiv - Plant Biology 2022Quote: ... 20 µl of protein extract was mixed with 180 µl of five-time diluted Bradford reagent (Bio-Rad, US) in a 96-well microplate ...