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Citations for Bio-Rad :
7701 - 7750 of 9281 citations for Nuclear Protein Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: 25 μl of the secreted proteins were ran on a Mini-PROTEAN® TGX™ precast gel (Bio-Rad). The whole lane of the gel for each sample was sliced into 4 mm sections and washed with 1 ml of 50% ethanol in 50 mM ammonium bicarbonate (ABC) ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were then electroblotted onto a PVDF membrane using a semidry blotter (Trans-Blot Turbo Transfer System; Bio-Rad). Membranes were blocked overnight at 4 °C in TBS-T (50 mM Tris-HCl ...
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bioRxiv - Plant Biology 2022Quote: The input or eluted proteins were separated by SDS-polyacrylamide-gel electrophoresis (PAGE) gels using Gel Electrophoresis (Bio-Rad), and blotted onto polyvinylidene fluoride (PVDF ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were blotted onto a nitrocellulose membrane using the Trans-Blot Turbo TRA Transfer Kit (Bio-Rad, Feldkirchen, Germany). Following protein transfer ...
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bioRxiv - Physiology 2023Quote: ... and isolated protein complexes were eluted from the beads in 1X SDS sample buffer (Bio-Rad Laboratories, Hercules, CA) at 60°C for 10 minutes ...
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bioRxiv - Synthetic Biology 2023Quote: ... Immunoblot was performed by transferring protein to a PVDF membrane using Trans-Blot Turbo RTA Transfer Kit (Biorad, 1704272) and the Biorad Transblot Turbo System ...
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bioRxiv - Neuroscience 2022Quote: Two thirds of eluted protein sample from HA Immunoprecipitation were run on a 4-15% Mini-PROTEAN (Bio-Rad) gel to separate proteins and remove synthetic HA peptide ...
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bioRxiv - Physiology 2022Quote: ... proteins were separated by running the gel in an electrophoresis chamber (Mini-PROTEAN Tetra Vertical Electrophoresis Cell, Bio-Rad) at 200 V for 30 min ...
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bioRxiv - Physiology 2024Quote: ... 6 μg of protein from each sample was loaded into a 4-20% Mini-PROTEAN® TGX gel (BioRad) and proteins were separated by size using electrophoresis ...
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bioRxiv - Neuroscience 2024Quote: ... proteins were separated by 10% SDS polyacrylamide gel electrophoresis (SDS-PAGE) using a Mini-PROTEAN kit (1658001FC, Bio-Rad). Between 20 and 50 μg of protein per well were loaded ...
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Activation of XBP1s attenuates disease severity in models of proteotoxic Charcot-Marie-Tooth type 1BbioRxiv - Neuroscience 2024Quote: ... for high-sensitivity chemiluminescent protein detection with Uvitec gel analysis systems or using enhanced chemiluminescence (ECL) reagents (Bio-Rad) with autoradiography film (Kodak Scientific Imaging Film ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were quantified by Pierce Reducing Agent Compatible BCA Protein Assay and run on 4-20% TGX gels (Biorad). Membranes were probed with anti-FLAG M2 antibody (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... proteins were resolved by 8.5% SDS-PAGE gel electrophoresis and transferred to PVDF membranes (Bio-Rad, Mississauga, ON, Canada). Membranes were blocked with 5% non-fat milk powder in Tris-buffered saline- Tween (TBS-T ...
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bioRxiv - Cell Biology 2024Quote: Protein extracts dissolved in Lämmli sample buffer or SDS sample buffer were separated by SDS-PAGE (Biorad Mini Protean) and transferred to ethanol activated Amersham Hybond-P 0.45 µm PVDF membranes (Cytiva ...
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bioRxiv - Immunology 2024Quote: ... The indicated mass of protein was boiled for 5 minutes at 95°C with 4x Laemmeli Buffer (BioRad #1610747) supplemented with 2.5% (v/v ...
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bioRxiv - Plant Biology 2023Quote: ... Samples containing 6 µg total protein were resolved using pre-cast SDS-PAGE Any KD™ gels (BIO-RAD), transferred to a polyvinylidene difluoride membrane (Immobilon-FL 0.45 µm ...
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bioRxiv - Neuroscience 2024Quote: ... 7.5 μg protein was loaded onto precast 15-well 4-15% Mini-Protean TGX Stain Free Gels (Bio-Rad).
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bioRxiv - Microbiology 2024Quote: ... SDS-Page total protein content was imaged using standard Coomassie brilliant blue staining with Kaleidoscope molecular weight ladder (Biorad). Antibodies tested to visualize M ...
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bioRxiv - Molecular Biology 2024Quote: ... and the samples were separated on 4-20% or 4-15% Mini-PROTEAN TGX Precast Protein Gels (BioRad Laboratories). The gel was transferred to a PVDF membrane using the iBlot transfer system (ThermoFisher) ...
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bioRxiv - Molecular Biology 2024Quote: ... Equal amounts of protein samples were fractionated by electrophoresis through agarose gels and transferred to PVDF membranes (Bio-Rad). After blocking with 5% dry milk for 2h ...
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bioRxiv - Molecular Biology 2024Quote: Input lysates (5 μg of total protein) and entire elution products were combined with 4x Laemmli buffer (Bio-Rad) supplemented with 10% β-mercaptoethanol ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were visualized using fluorescent secondary antibodies and quantified using ChemiDoc MP device (Bio-Rad Laboratories, Hercules, CA, USA) and quantified with Image Lab software (version 6.0 ...
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bioRxiv - Neuroscience 2024Quote: ... Protein levels were visualized using ECL-based autoradiography film system (Super RX-N, Fujifilm) or ChemiDocTM Imaging system (BioRad) and analyzed using Gel Analyzer plugin from ImageJ (Fiji) ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were prepared for SDS-PAGE separation by denaturation with 2x Laemmli sample buffer (Bio-Rad Laboratories; 161-0737) with 355mM β-mercaptoethanol for 5min at 95°C.
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bioRxiv - Molecular Biology 2024Quote: ... under reducing conditions and proteins transferred to nitrocellulose membranes using a semidry Trans-Blot Turbo Transfer System (Bio-Rad). Antibody reactions were carried out in 1% skimmed milk in PBS with 0.1% Tween-20 and washed in PBS with 0.1% Tween-20 ...
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bioRxiv - Microbiology 2023Quote: Proteins from LEVs and SEVs were separated on a precast 4-15 % SDS-PAGE (Bio-Rad, Hercules, CA USA) and stained with Coomassie G-250 ...
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bioRxiv - Cell Biology 2024Quote: ... The pelleted protein G Sepharose beads were then resuspended in 50 µl of 2x Laemmli sample buffer (Bio-Rad) and proceeded to SDS-PAGE and immunoblotting analysis.
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bioRxiv - Immunology 2024Quote: ... Total protein concentrations were measured using Pierce BCA Protein Assay Kit and equal amount of protein (10-20μg) mixed with Laemmli sample buffer were loaded in 4-20% TGX precast gels (Biorad) for gel electrophoresis ...
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bioRxiv - Cell Biology 2023Quote: ... Equal amount of protein samples was separated on SDS-PAGE gel (12%) electrophoresis and transferred to nitrocellulose membrane (BioRad). Blots were blocked with 5% skim milk for 1 hour at room temperature and probed with antibodies at 4℃ overnight against the desired proteins ...
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bioRxiv - Cell Biology 2024Quote: Anti-Flag antibody precipitated proteins in the concentrated eluate were resolved in a 4-15% Criterion TGX gel (BioRad) and stained by Colloidal Blue dye (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: ... proteins were transferred onto membranes using Trans-Blot® TurboTM RTA Midi 0.2 µm Nitrocellulose Transfer Kit (Biorad, #1704271). The membranes were subsequently blocked in TBST solution (200 mM Tris-HCl ...
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bioRxiv - Developmental Biology 2024Quote: ... Proteins in cell lysates were heated at 95[ for 5 minutes in Laemmli samples buffer (Bio-Rad, Hercules, CA), resolved by 10% SDS-PAGE ...
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bioRxiv - Cell Biology 2023Quote: ... Protein samples were separated on Mini-PROTEAN® TGX™ Precast Gels (4–20% gradient, BioRad Cat. No. 4561091) in Tris-SDS-glycine buffer ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were transferred to nitrocellulose membranes (GE Amersham) by semi-dry transfer for 10 min at 15V (Bio-Rad), air dried ...
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bioRxiv - Immunology 2024Quote: ... and an equal amount of protein was resolved in 4-12% SDS-polyacrylamide gel electrophoresis (SDS-PAGE) (Bio-Rad). Proteins were transferred to polyvinylidene difluoride membranes (Millipore ...
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bioRxiv - Bioengineering 2023Quote: ... Proteins (20 μg per sample) were separated on Mini-PROTEAN TGX 4–15% precast SDS-PAGE gels (Bio-Rad) for 1–3 hours at 100 V and then transferred to PVDF membrane (Bio-Rad ...
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bioRxiv - Biochemistry 2024Quote: ... Elution fractions containing pure protein were determined via SDS-PAGE analysis on a 4-20% gradient gel (Bio-Rad), pooled ...
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bioRxiv - Biochemistry 2024Quote: ... The resolved proteins were transferred onto nitrocellulose membranes (Amersham) through a Trans-Blot semi-dry transfer cell (Bio-Rad). Membranes were subsequently blocked in Tris-buffered saline plus 0.1% Tween (TBS-T ...
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bioRxiv - Cell Biology 2024Quote: ... Protein band intensities were quantified using ChemiDoc MP Imaging System and Image Lab software (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Cell Biology 2024Quote: ... The gel in Figure 7B was run on a 12% Mini-Protean TGX precast protein gel (Bio-Rad, #4561034), whereas the one in Figure 7C was run using hand-cast gels ...
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bioRxiv - Microbiology 2023Quote: ... Samples were then analyzed by 4-20% SDS-PAGE gels (Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad™) alongside size standards (Precision Protein Plus ...
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bioRxiv - Neuroscience 2023Quote: ... Separated proteins were blotted on a polyvinylidine fluoride (PVDF) membrane with a Trans-Blot Turbo Transfer System (Bio-Rad). The protein coated membrane was blocked with 5% normal serum (Sigma ...
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bioRxiv - Genetics 2023Quote: ... ∼25 μL sample was run out on a 7.5% SDS-PA at 150V for ∼1 h alongside a protein standard (BioRad Precision Plus Protein Dual Color Standard) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Soluble proteins contained in the supernatant were separated and mixed with 4x Laemmli sample buffer (Bio-Rad, 161-0747) before being loaded on a SDS-PAGE gel (4–20% Criterion™ TGX Stain-Free™ Protein Gel ...
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bioRxiv - Physiology 2023Quote: ... stain-free gels were imaged following UV exposure to visualise total protein content (ChemiDoc MP Imaging System, Bio-Rad). Proteins were transferred from the gel to an ethanol-immersed PVDF membrane in transfer buffer (30 min ...
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bioRxiv - Microbiology 2023Quote: ... The protein samples (10 μL each) were run on 4-20 % Mini-PROTEAN TGX Stain-FreeTM precast Gels (BioRad) in 1× TGX buffer and then transferred to nitrocellulose membrane using a Trans-Blot® Turbo™ Transfer System (BioRad) ...
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bioRxiv - Neuroscience 2023Quote: ... 25 µl cell lysate and 50 µg protein for tissue lysates was loaded on 4-15% (Bio-Rad, 5678084) and 10% TGX Stain-Free Protein Gels (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were transferred to nitrocellulose membranes via semi-dry transfer (Bio-Rad Trans Blot Turbo; 25 V, 30 min) for western immunoblot analysis ...
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bioRxiv - Neuroscience 2023Quote: ... stain-free gels were imaged following UV exposure to visualise total protein content (ChemiDoc MP Imaging System, Bio-Rad). Alternatively ...
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bioRxiv - Immunology 2023Quote: ... and Tris-Glycine SDS buffer (Geneflow B9-0032) or 4–12% Criterion™ XT Bis-Tris Protein Gels (BioRad) and XT MES running buffer (BioRad 1610789) ...