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Citations for Bio-Rad :
701 - 750 of 1482 citations for pIEXBac c EGFP 3 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... The cells were centrifuged for 15 min at 15,000 x g at 4°C and the supernatant was quantified with Quick Start Bradford 1X Dye Reagent (BioRad), using an albumin curve to determine the concentration of protein to be placed ...
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bioRxiv - Cell Biology 2024Quote: ... and heated to 98°C for 5 min and transferred to Trans-Blot PVDF Transfer membranes (Bio-Rad, #1704157). Membranes were blocked for 1 h at room temperature with TBS-T (0.1% Tween 20 ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were heated to 95°C with 2X sample buffer for 5 mins before loading the samples directly to a 0.2μm nitrocellulose (BioRad, #1704159) membrane ...
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bioRxiv - Molecular Biology 2023Quote: ... at 95°C for 10 min and separated by electrophoresis in a 4–12% SDS–PAGE gel (Bio-Rad) and transferred to a PVDF membrane (Bio-Rad) ...
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bioRxiv - Molecular Biology 2022Quote: ... Plates were incubated for 18 to 24 h at 37°C and imaged in a ChemiDoc Imaging System (BioRad) to detect RFP and GFP fluorescence (Cy3 and Cy2 channels ...
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bioRxiv - Microbiology 2022Quote: ... reagent in 0.2 mL PCR 8-well strips and heated to 100 °C for 10 min (T100; BioRad, Australia) and used immediately ...
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bioRxiv - Cancer Biology 2023Quote: ... The eluent was isolated by centrifugation at 100 g for 4 min at 4°C (Bio-Rad 732-6204), buffer exchanged (Amicon Ultra 10 kDa MWCO UFC501024 ...
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bioRxiv - Physiology 2023Quote: ... and transferred at 4°C for 16 hrs at 30 V onto 0.45 µm supported nitrocellulose membrane (Bio-rad). Membranes were blocked in EveryBlot Blocking Buffer (Bio-rad ...
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bioRxiv - Cell Biology 2023Quote: ... boiled 10 min at 95°C and run in 4% - 15% Mini-Protean TGX Stain-Free gels (Bio-Rad) in non-reducing conditions (no β-mercaptoethanol nor DTT) ...
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bioRxiv - Microbiology 2023Quote: ... 1 minute extensions and a final hold temperature of 12 C using a C1000 Touch Thermal Cycler (BioRad #1851197). Samples were subsequently loaded into the QX200 Droplet Reader for quantification ...
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bioRxiv - Microbiology 2023Quote: ... The resulting mixtures were put at 37°C for 30min and transferred to 0.4 cm electroporation cuvettes (Gene Pulser/MicroPulser, BioRad), which were submitted to electroporation using 10ms pulses at 180V repeated three times at one-second intervals ...
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bioRxiv - Molecular Biology 2023Quote: ... Total proteins were centrifuged (15000 rpm at 4°C) and determined using protein assay dye reagent (Bio-Rad, 500006). Subsequently ...
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bioRxiv - Neuroscience 2023Quote: ... Protein extracts were denatured 5 min at 95°C in a Laemmli buffer (Bio-Rad, Marnes-la-Coquette, France) with β-mercaptoethanol (1/10 ...
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bioRxiv - Neuroscience 2023Quote: ... were centrifuged at 20,000 x g for 25 min at 4⁰C and supernatants were assayed for total protein (Bradford assay, BioRad). Aliquots of WCL (30 µg total protein ...
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bioRxiv - Microbiology 2024Quote: ... samples were incubated at 95°C for 10LJminutes and then resolved on a TGX Stain-free gel (Bio-Rad). The proteins were transferred onto 0.2LJµm nitrocellulose membranes using Trans-Blot Turbo Transfer (Bio-Rad ...
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bioRxiv - Cancer Biology 2024Quote: ... slides were incubated at 60°C for 2 h on a Xenium thermocycler adaptor plate (BioRad, C1000 Touch Thermocycler), followed by deparaffinized through a series of immersions in xylene ...
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bioRxiv - Molecular Biology 2024Quote: ... 58°C for 30 sec using a CFX ConnectTM real-time PCR detection system (Bio-Rad, Philadelphia, PA, USA). List of genes validated and corresponding primer sequences are given in Table S8.
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bioRxiv - Cancer Biology 2024Quote: ... which were boiled at 95°C for 5 minutes before loading in pre-cast PAA gels (Bio-RAD, 4561084) and run at 100V for approx ...
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bioRxiv - Microbiology 2024Quote: ... we incubated the samples 5 min at 65°C in a C1000 thermal cycler (Bio-Rad, Hercules, CA, USA). We added a synthesis mixture of 10 mM deoxynucleoside triphosphates ...
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bioRxiv - Plant Biology 2024Quote: ... followed by a melt curve from 95 to 55°C) was run on the CFX Thermo Cycler (Bio-Rad). Non-baseline corrected data was analyzed in LinRegPCR23 software (version 2021.2 ...
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bioRxiv - Molecular Biology 2024Quote: ... The lysate was clarified and incubated at 55°C for 5 minutes with Laemmli sample buffer (1610747, Bio-Rad). Samples were run on a 4–15% Mini-PROTEAN™ TGX Stain-Free™ Protein Gel (4568086 ...
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bioRxiv - Immunology 2024Quote: ... with a final step at 72°C for 10 min in a T100™ Thermal Cycler instrument (Bio-Rad). DNA oligo template sequences for circAEBP2 probe and random probe and PCR primer sequences are listed in Supplementary Table 1.
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bioRxiv - Bioengineering 2024Quote: ... followed by a 3h incubation at 42°C on a Xenium Thermocycler Adapter plate positioned in a thermocycler (BioRad). Subsequently ...
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bioRxiv - Cancer Biology 2024Quote: ... slides were incubated at 60°C for 2 h on a Xenium thermocycler adaptor plate (BioRad, C1000 Touch Thermocycler), followed by deparaffinized through a series of immersions in xylene ...
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bioRxiv - Genetics 2024Quote: ... at 4°C in a cold room using transfer stacks (from Trans-Blot Turbo RTA kit, Bio-Rad, 1704272). Next ...
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bioRxiv - Cancer Biology 2024Quote: ... Each fraction of the C-DGUC purified sEVs (37.5 μL) was mixed with 12.5 μL of 4 × Laemmli Sample Buffer (Bio-Rad) and 10% 2-Mercaptoethanol (Bio-Rad) ...
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bioRxiv - Genetics 2024Quote: ... samples were heated at 95°C for 4min and separated on 15% denaturing polyacrylamide gels (ratio acrylamide:bis acrylamide 19:1 [Biorad]). After fixing in a solution (40% methanol ...
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bioRxiv - Cancer Biology 2021Quote: ... 3 µg of total RNA was used in the reverse transcription reaction using the iScript cDNA synthesis kit (Bio-Rad). Quantitative PCR amplification was performed on the Prism 7900 Sequence Detection System (Applied Biosystems ...
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bioRxiv - Cell Biology 2021Quote: ... DNA samples (3 μl) and master mixes (7 μl) were pipetted into a 96-well PCR plate (Bio-Rad #MLL9651) and PCR amplification performed using the CFX96 Touch Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: ... and 3 mg bromophenol blue) and separated by SDS-PAGE using 4-to-20% gradient polyacrylamide gels (Bio-Rad Laboratories) at 10 mAmps for 16 h ...
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bioRxiv - Molecular Biology 2021Quote: ... 5’KI and 3’KI as detailed (Fig 2A and D) with the SsoAdvanced Universal SYBR Green Supermix (Bio-Rad). Briefly ...
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bioRxiv - Immunology 2020Quote: Densitometric analysis of cleaved caspase 3 immunoblots from three independent experiments were performed using the VersaDoc Imaging System (Bio-Rad) and analyzed with ImageJ 1.52p Fiji package software (https://imagej.net/Fiji) ...
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bioRxiv - Microbiology 2022Quote: ... Ten micrograms of total RNA were separated on a 1% agarose gel containing 1X 3-(N-morpholino)propanesulfonic acid (MOPS) buffer and 2.2 M formaldehyde and transferred to a Zeta-probe membrane (Bio-Rad) by capillary transfer in 20X SSC buffer (3 M NaCl ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein bound GST beads were washed 3 times in the GST lysis buffer by centrifugation at 1,000 rcf for 3 minutes and resuspended in 4X Laemmli sample buffer (Bio-Rad), heat denatured and centrifuged at 1,000 rcf for 3 minutes ...
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bioRxiv - Microbiology 2020Quote: ... Cells were centrifuged for 5 min at 7,000 g and washed twice with 200 μL of 1x PBS containing 3% BSA and 0.1% (v/v) Tween-20 (BioRad, Germany). Mounting of cells for STED microscopy was performed as described above.
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bioRxiv - Cancer Biology 2021Quote: ... Scientific until approximately 3 cm of separation was obtained between the 25 and 37 kDa protein standards (Bio-Rad; 1610375). Using electrophoresis ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were in-gel rehydrated for 16 hrs and isoelectrically focused on 7 cm pH 3-10 IPG strips to 10,000 Vh on a Protean® IEF Cell (BioRad). After focusing ...
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bioRxiv - Molecular Biology 2020Quote: ... Each reaction contained 3 technical replicates and were carried out using a CFX384 TouchTM Real-Time PCR Detection System (BioRad Laboratories Ltd. ...
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bioRxiv - Microbiology 2020Quote: ... Samples were run in 3-12% Bis-Tris gradient gels and transferred to PVDF membranes in a wet blot system (BioRad). Membranes were dried ...
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bioRxiv - Microbiology 2020Quote: ... were mixed in a 1000:3 ratio and added to the blots prior to visualization via Chemidoc Touch Imaging system (BioRad). For OmpT visualization ...
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bioRxiv - Cell Biology 2020Quote: ... were added for 4 h and then samples were centrifuged at 10,000g for 3 minutes and washed three times with RIPA buffer and re-suspended in laemmli buffer (Bio-Rad), boiled for 5 minutes ...
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bioRxiv - Microbiology 2022Quote: ... Intact cells were divided into 100-μl aliquots and heated individually at different temperatures for 3 minutes in a PCR machine (Biorad), followed by cooling for 2 minutes at room temperature ...
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Proteolytic cleavage of the extracellular domain affects signaling of parathyroid hormone receptor 1bioRxiv - Pharmacology and Toxicology 2022Quote: ... Lysates were cleared by centrifugation and run on 10% SDS-polyacrylamide gels in a Mini-PROTEAN 3 cell apparatus (Biorad). Proteins were electroblotted onto Immobilon P membranes (Millipore ...
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bioRxiv - Molecular Biology 2022Quote: ... Ten micrograms of total RNA were separated on a 1% agarose gel containing 1X 3-(N-morpholino)propanesulfonic acid (MOPS) buffer and 2.2 M formaldehyde and transferred to a Zeta-probe membrane (Bio-Rad) by capillary transfer in 20X SSC buffer (3 M NaCl ...
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bioRxiv - Neuroscience 2021Quote: ... Between 3 and 10 μg of total protein were loaded on to a 4-15% gradient TGX gel (Bio-Rad) and transferred on to a PVDF membrane (Bio-Rad) ...
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bioRxiv - Developmental Biology 2020Quote: ... Western blots were performed either with Criterion ™ XT Tris-Acetate Precast Gels 3–8 % (3450130, Bio-Rad, Hercules, CA), XT Tricine running buffer (161–0790 ...
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bioRxiv - Microbiology 2021Quote: ... Primers (Table 3) were designed using Primer3 Plus (31, 32) to quantify transcripts using Universal SYBR Green Supermix (Bio-Rad) using a Quantstudio 6 Flex (Applied Biosystems ...
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bioRxiv - Cancer Biology 2021Quote: ... 3 μg of total RNA was used in the reverse transcription reaction using the iScript cDNA synthesis kit (Bio-Rad). Quantitative PCR amplification was performed on the Prism 7900 Sequence Detection System (Applied Biosystems ...
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bioRxiv - Cell Biology 2021Quote: ... ± phosphatase inhibitor cocktail 3) and incubated for 30 min on ice before addition of MnCl2 and λ-phosphatase (Bio-Rad) to the appropriate samples for 30 min at 30°C ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed with PBS-T 3 times after each step and finally imaged on the ChemiDoc MP Imaging System (Biorad).