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Citations for Bio-Rad :
651 - 700 of 1482 citations for pIEXBac c EGFP 3 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... Blots were washed 3 times with TBST for 5 min and visualized using a ChemiDoc MP Imaging System (Biorad).
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bioRxiv - Immunology 2023Quote: ... in non-reducing conditions and run at 120 V in 3-Morpholinopropane-1-sulfonic acid (MOPS) buffer (Bio-rad). Bands were visualized with Imperial Protein Stain (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... The membrane was again washed 3 times with TBST and then placed in Clarity Max Western ECL (Bio-Rad) substrate for 5 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... All reactions were performed in 3 technical replicates using the CFX96 Touch Real-Time PCR Detection System (Bio-Rad). Data was analysed according to the 2-ΔΔCT method52.
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bioRxiv - Biochemistry 2023Quote: ... in non-reducing conditions and run at 120 V in 3-Morpholinopropane-1-sulfonic acid (MOPS) buffer (Bio-Rad). Bands were visualized with Imperial Protein Stain (Thermo Fisher Scientific) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... All cDNA samples (3 biological replicates) were run in SsoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad, 1725271) in 3 technical replicates for 40 cycles ...
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bioRxiv - Biophysics 2023Quote: ... Blotting was done by standard mini-TGX protocol (2.5 A, 25 V, 3 minutes) using a Trans-blot Turbo Transfer System (BioRad). Membranes were incubated with UBQLN2 primary antibodies (1:5000 diluted ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Beads were washed by lysis buffer for 3 times and boiled in 2X Laemmli Sample buffer (Bio-RAD, #1610737). Monoclonal ANTI-FLAG M2-HRP antibody (Sigma A8592 ...
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bioRxiv - Cell Biology 2020Quote: ... Beckton Dickinson) overnight at 4°C and visualized using the Bio-Rad ChemiDoc MP Imaging System (Bio-Rad Laboratories).
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bioRxiv - Developmental Biology 2021Quote: ... Sections were incubated overnight at 4 °C with rabbit anti-chick IL-6 (Bio-Rad Laboratories, Inc., Hercules, CA) diluted 1:20 in incubation buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... at 100 V for 1 hr in 4°C pre-chilled 1X Tris-Glycine buffer (Bio-Rad 161-0734). Membranes were blocked for 1 hr at room temperature in blocking buffer (PBS (Gibco 14190-144) ...
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bioRxiv - Developmental Biology 2020Quote: ... the membrane was blocked overnight at 4°C using 5% non-fat dry milk (Bio-Rad, catalog 170-6404) in Tris-buffered saline pH 7.3 ...
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bioRxiv - Biochemistry 2020Quote: ... The reconstitution sample was nutated for 1 h at 4°C before addition of 0.2 g/mL SM2-BioBeads (BioRad), and the reconstitution sample was further nutated overnight at 4°C before removal of the biobeads ...
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bioRxiv - Bioengineering 2021Quote: ... and final extension step at 72 °C for 20 s using the CFX384 Real-Time System from Bio-Rad, Hercules (CA ...
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bioRxiv - Bioengineering 2021Quote: ... and probed overnight at 4°C with IRDye 680 Streptavidin (LICOR) diluted 1:3000 in 1:1 TBST:Blocking Buffer (BioRad). Membranes were imaged using an Azure Biosystems c600 ...
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bioRxiv - Cell Biology 2021Quote: ... The N-ter (pI 5.5) and C-ter (pI 9.9) peptides were conjugated to Affi-Gel-10 (Bio-rad) agarose beads for affinity-purification ...
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bioRxiv - Microbiology 2020Quote: ... The cell lysates were subjected for centrifugation at 13,000 rpm for 30 min at 4°C and the supernatant were incubated in sample loading buffer 2x (Biorad) containing 10% β-mercaptoethanol for 5 min at 98 °C ...
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bioRxiv - Microbiology 2021Quote: ... the plates were sealed and incubated at 37°C for 2 h in a T100 Thermal Cycler (Bio-Rad).
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bioRxiv - Microbiology 2020Quote: ... 7.5 mg of proteins were diluted in ChIP buffer supplemented with 0.01% SDS and precleared 1 h at 4 °C with 50 μl of protein A agarose beads (BioRad) and 100 μg BSA ...
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bioRxiv - Cell Biology 2021Quote: ... heated for 10 min at 95°C and loaded on Mini Protean TGX precast gel 4-12% (Bio-Rad). For the blot ...
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bioRxiv - Neuroscience 2020Quote: ... The mixture was incubated at 37°C in PCR tubes on a thermal cycler (BioRad DNA Engine PTC-200). After various times 50 µl of the mixture was taken and subject to SEC using a Shodex KW-802.5 column (HICHROM ...
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bioRxiv - Bioengineering 2022Quote: ... droplets were left at 4 °C for at least 30 minutes and read using the QX200 Droplet Reader (BioRad). ddPCR data was analyzed using QuantaSoft™ software ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by cross-linking using a UV Stratalinker and drying at 80°C on a Gel Dryer (Bio-Rad). The membrane was probed using a T4 Polynucleotide Kinase (PNK ...
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bioRxiv - Cancer Biology 2022Quote: ... samples were heated for 5’ at 95°C in Lämmli buffer and protein lysates were resolved on polyacrylamide Mini-PROTEAN TGX gels (BioRad) and transferred onto nitrocellulose membrane by wet-transfer ...
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bioRxiv - Neuroscience 2022Quote: ... Lysates were then centrifuged at 12,000 rpm at 4°C for 20min and total protein extracts were boiled in Laemmli sample buffer (BioRad), separated by SDS-PAGE under reducing conditions and transferred to PVDF membranes (Millipore) ...
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bioRxiv - Cancer Biology 2022Quote: ... 150 mM KCl and 2 mM MgCl2) at 37°C for 16 hours in thermal cycler (Bio-Rad, USA). The samples were separated using 10% non-denaturing polyacrylamide gel and tris/boric acid/EDTA buffer (TBE ...
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bioRxiv - Biophysics 2022Quote: ... Mouse anti-tubulin b3 antibody which recognizes the C-terminal tail of β tubulin was purchased from Bio-Rad Laboratories.
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bioRxiv - Microbiology 2020Quote: ... in 0.5×TBE buffer at 4 to 7 °C for 260 h using a CHEF Mapper System (Bio-Rad). Switching time was 1,200 to 4,800 s at 1.5 V/cm with an included angle of 120° ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was incubated at 4°C for 1 h with Bio-Beads™ SM-2 resin (Bio-Rad) equilibrated with 100 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2020Quote: ... The immunoprecipitated proteins were eluted by heating at 100°C during 15 minutes in 4x Laemmli Sample Buffer (BIORAD). Immunoprecipitated protein complexes were subjected to Western blot analysis ...
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bioRxiv - Microbiology 2020Quote: ... which was inoculated at 30°C for 1 hour and then photographed using a UV transilluminator (Bio-Rad, USA).
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bioRxiv - Microbiology 2021Quote: ... SOB motility plates (containing 10-4 M IPTG and 2.4 g/L MgSO4) at 37°C and pictures were taken using a Gel Doc (Biorad) imager at the beginning and the end of the linear swimming motility period (from 5 to 8 hours) ...
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bioRxiv - Cell Biology 2020Quote: Immunoprecipitated proteins were resuspended in SDS-PAGE sample buffer and heated 5 minutes at 95°C before loading onto a 7.5% acrylamide mini-Protean TGX gel (BioRad). After 7 minutes of electrophoresis at 100 V the gel was stained with Imperial Protein stain (Thermo ...
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bioRxiv - Genomics 2021Quote: ... They were then boiled for 5 minutes at 99°C after the addition of Laemmli buffer (Cat. #1410737, Biorad). Proteins were separated by SDS-PAGE and transferred to a membrane with a Trans Blot TURBO (Biorad) ...
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bioRxiv - Biochemistry 2021Quote: ... Plates were incubated ≈20-24 hours at 37°C and then photographed using a ChemiDoc XRS+ System (Bio-Rad). At least three independent transformants were used to perform replicates for phenotypic assays.
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bioRxiv - Biochemistry 2021Quote: ... the samples were gradually heated from 4 to 98°C with a rate of 0.2°C/10 s in the CFX96 Real-Time System C1000 Touch Thermal Cycler (Biorad). The fluorescence intensity was measured using an excitation wavelength of 470 nm while monitoring emission at 570 nm.
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bioRxiv - Biochemistry 2021Quote: ... The enriched protein were eluted by heating at 95°C for 30 minutes in 1X Laemmli sample buffer (BioRad) and processed to western blot ...
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bioRxiv - Bioengineering 2021Quote: ... Membranes were stained overnight at 4°C with a 1:1000 diluted mouse anti-his primary antibody (MCA1396, RRID:AB_322084, Bio-Rad) and then for 1 hour at room temperature with a 1:4000 diluted rabbit anti-mouse HRP secondary antibody (SouthernBiotech Cat# 6170-05 ...
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bioRxiv - Genetics 2020Quote: ... 0.2 mM EDTA) at 95 °C for 30 min in a thermal cycler (BIORAD C100, BIORAD, Hercules, CA, USA), then 20 μl of neutralization buffer (40 mM TrisHCl ...
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bioRxiv - Neuroscience 2020Quote: ... Lysates were cleared of debris by centrifugation at 15,000 x g for 15 min at 4°C and then normalized for protein content using Bradford assay (BioRad). Normalized protein lysates were fractionated by 10 % SDS/PAGE and transferred onto a PVDF membrane (GE Healthcare Life Sciences ...
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bioRxiv - Plant Biology 2022Quote: ... 4 °C) and the supernatant was taken up and the protein concentration was determined using the Bradford assay (Biorad).
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bioRxiv - Molecular Biology 2022Quote: ... followed by cross-linking using a UV Stratalinker and drying at 80°C on a Gel Dryer (Bio-Rad).
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bioRxiv - Synthetic Biology 2022Quote: ... The reaction was run for 7 h at 34 °C on a CFX96 real-time PCR detection system (Biorad) and monitored thanks to the SYBR Green II signal.
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bioRxiv - Synthetic Biology 2024Quote: ... followed by incubation at 37 °C for up to 7 hours on a real-time qPCR instrument (Biorad, CFX96). To measure the time-dependent change in fluorescence ...
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bioRxiv - Biochemistry 2024Quote: ... 0.1% SDS for 10 min at 60°C and images captured using a ChemiDoc MP Imaging system (BIO-RAD).
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bioRxiv - Immunology 2024Quote: ... The indicated mass of protein was boiled for 5 minutes at 95°C with 4x Laemmeli Buffer (BioRad #1610747) supplemented with 2.5% (v/v ...
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bioRxiv - Neuroscience 2024Quote: ... by boiling for 10 min at 98°C before loading onto a 4-20% gel (Bio-Rad, Hercules, CA), then transferred to PVDF 0.22 m membrane (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... 2 min at 72°C for final extension in a T100 Thermal Cycler (BIO-RAD Lab. Inc., Hercules, CA). The sizes of PCR products were checked by running agarose gel electrophoresis ...
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bioRxiv - Molecular Biology 2023Quote: ... Pulse-field gel electrophoresis was carried out in a 0.9% agarose gel in 0.5× TBE at 14°C with a CHEF DR III from Bio-Rad with a constant switch time of 20 s during 24 h ...
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bioRxiv - Microbiology 2024Quote: ... Samples were heated for 10 mins at 70°C and loaded onto 4-20% polyacrylamide gradient gels (Bio-Rad) for electrophoretic separation in Tris-glycine-SDS buffer (Bio-Rad) ...