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Citations for Bio-Rad :
501 - 550 of 1482 citations for pIEXBac c EGFP 3 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... ramp rate set at 2 °C/sec] was performed in a thermal cycler (Bio-Rad C1000 Touch). Droplets were quantified in a QX200 Droplet Reader (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... and then incubated for at least 16 hours at 4°C with either mouse monoclonal anti-His (1:2,000; AD1.1.10, Biorad) or mouse anti-RNA pol (1:5,000 ...
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bioRxiv - Developmental Biology 2022Quote: ... Supernatant was discarded and the RFP+ cells were frozen (−80°C) in PureZol (Bio-Rad. #732-6890). Triplicates of RNA from RFP+ cells were purified as described above.
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bioRxiv - Biochemistry 2022Quote: ... gels were dried at 80 °C for 1 h in a gel dryer (Model 583, Bio-Rad), exposed to phosphorimager plates and signal acquired in Typhoon Trio™ Variable Mode Imager (GE Healthcare) ...
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bioRxiv - Microbiology 2022Quote: ... 4°C) and protein content was measured by Bradford assay using Protein Assay Dye Reagent Concentrate (BioRad). 20 μg total protein were denatured (10 min ...
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bioRxiv - Molecular Biology 2022Quote: ... and 70°C (30 s) with 40 cycles (CFX384™ Real-Time PCR Detection System, Bio-Rad). The relative expression of the studied miRs was calculated using equation 2−Δ Cq ...
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bioRxiv - Biochemistry 2021Quote: ... for 16 hours at 4°C before being applied to a gravity flow Econo-column (Bio-Rad). Beads were washed thoroughly with 20 column volumes of binding buffer followed by 10 column volumes of binding buffer supplemented with an additional 12.5 mM imidazole ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.3 mM EDTA) at 4°C and transferred overnight to a Zeta-Probe nylon membrane (Bio-Rad) at 20 V and 4°C in a Hoefer Transphor TE 42 unit ...
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bioRxiv - Neuroscience 2020Quote: ... 70°C for 10 min) on a Peltier Thermal Cycler (Bio-Rad Laboratories Inc., Hercules, CA, USA) using the GoTaq® 2-step RT-qPCR kit (Promega) ...
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bioRxiv - Cell Biology 2021Quote: ... at 47V overnight at 4°C using Mini Trans-Blot® Cell (Bio-Rad Laboratories, CA, USA). Following the transfer ...
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bioRxiv - Neuroscience 2021Quote: ... Aliquots of protein were boiled at 95°C in 2X Lamelli buffer (Bio-Rad, Hercules, CA, USA) for 5 min ...
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bioRxiv - Biochemistry 2021Quote: ... Blots were then blocked for 1 h at 25 °C with 5% non-fat dry milk (BioRad) in TBST (50 mM Tris ...
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bioRxiv - Microbiology 2021Quote: ... and final extension at 72°C for 7 min using a C1000 Touch Thermal Cycle (BIO-RAD). PCR results were run on 1% agarose gels and imaged using an iBright CL1500 Imaging system (ThermoFisher Scientific).
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bioRxiv - Immunology 2021Quote: ... They were incubated overnight at 4°C with primary Ab (anti-bovine MHCII clone MCA5655 from BioRad, anti-bovine pancytokeratine clone BM4068 from Acris ...
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bioRxiv - Biochemistry 2021Quote: ... After drying the gel (80 °C for 1.5 h) using a Model 583 Gel DRYER (Bio-Rad), it was exposed to a Phosphor Screen (GE Healthcare ...
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bioRxiv - Cancer Biology 2022Quote: ... and 10 minutes at 72°C using the C1000 Touch Thermal Cycler (Bio-Rad, Los Angeles, CA). For Sftpc gene ...
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bioRxiv - Cancer Biology 2022Quote: ... and 2 minutes at 72°C using the C1000 Touch Thermal Cycler (Bio-Rad, Los Angeles, CA). Primer sequences are listed in the Supplemental Methods.
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bioRxiv - Molecular Biology 2022Quote: ... Pallets were then heated at 95°C for 5 min with Laemmli buffer (Cat#1610747; Biorad, USA) and centrifuged at 15,000g for 10 minutes to collect the supernatant ...
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bioRxiv - Molecular Biology 2022Quote: ... The gel was dried for 1 h at 80 °C on a Gel Dryer 583 (Bio-Rad) and visualized after appropriate exposure on a Phosphorimager (FLA-3000 Series ...
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bioRxiv - Molecular Biology 2024Quote: ... C) 5’-CTTCAAGGAGGACTGCCAC & and 5’-TGGGGTAGGTGCCGAAGT) and target concentration was determined using QuantaSoft Software™ (Bio-Rad).
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bioRxiv - Neuroscience 2024Quote: ... and heated at 95°C for 7 min prior to separation by 10% SDS-PAGE (BioRad Laboratories). Following electrophoresis ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were eluted from beads at 95°C for 5min using 4X Laemmli Sample Buffer (Bio-Rad). Denatured samples from both the total lysate (20µg ...
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The RNA-binding protein RbpB is a central regulator of polysaccharide utilization in gut BacteroidesbioRxiv - Microbiology 2023Quote: ... The gel was dried at 80°C for 1 h on a Gel Dryer 583 (Bio-Rad), exposed over night ...
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bioRxiv - Neuroscience 2024Quote: ... or at 100 V for 2 h at 4 °C using the wet transfer method (Bio-Rad Mini Trans-Blot Electrophoretic Cell 170-3930) ...
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bioRxiv - Biochemistry 2024Quote: ... Detergent removal was carried out at 4°C via incubation with Bio-Beads SM-2 (Bio-Rad) at a concentration of 200 mg ml-1 for three times with two hours for each time ...
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bioRxiv - Cell Biology 2023Quote: ... which were boiled at 95°C for 5 min before being separated using SDS-PAGE (4561086, Biorad).
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bioRxiv - Microbiology 2023Quote: ... All steps were done at 4 °C and concentrations of all proteins were determined by Bio-Rad Protein Assay Dye Reagent Concentrate.
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bioRxiv - Synthetic Biology 2023Quote: ... The reaction mixture was incubated in a thermal cycler (Bio-Rad C1000 thermal cycler, 105°C lid) with the protocol ...
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bioRxiv - Microbiology 2023Quote: ... 4°C) and protein content was measured by Bradford assay using Protein Assay Dye Reagent Concentrate (BioRad). 20 µg total protein were denaturated (10 min ...
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bioRxiv - Bioengineering 2023Quote: ... 4°C for 45 min was loaded into the glass chromatography columns (Bio-Rad, catalog number 7372522) and incubated with Ni-NTA agarose resin (Qiagen ...
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bioRxiv - Synthetic Biology 2023Quote: ... Gels were then electroblotted at 0 °C in Towbin buffer (70) to PVDF membrane (Immun-Blot, Biorad) for 600 minutes at 30 V ...
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bioRxiv - Neuroscience 2023Quote: ... slides were heated at 37 °C for 1 min on a T100 thermal cycler (BioRad, Hercules, CA), transferred to precooled methanol (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... and incubating the samples at 95°C for 5 min in a thermal cycler (Bio-Rad, USA) and decreasing the temperature of 1.5°C/min until it reaches 25°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell debris was pelleted by microcentrifugation at 4°C and protein levels quantified using Bradford Assays (BioRad) as previously described (38) ...
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bioRxiv - Biochemistry 2023Quote: ... the flow chamber was incubated at 4°C overnight with 25 ng/µL Digoxigenin Antibody (Bio-Rad) that adsorbed onto the polystyrene-covered lower surface ...
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bioRxiv - Microbiology 2024Quote: ... Plates were incubated at 37°C and imaged over time using ChemiDoc MP imaging system (Bio-Rad).
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bioRxiv - Microbiology 2024Quote: ... samples were incubated at 95°C for 10 and loaded onto TGX stain-free gels (Bio-Rad) for SDS-PAGE ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were digested in 100µL 10% Chelex (Bio-Rad 142–1253; 20 minutes 95°C, 2x vortex) and centrifuged (2 minutes 12,000 RPM ...
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bioRxiv - Biochemistry 2024Quote: ... the beads were heated at 50°C in 50 µL of 2x Laemilli sample buffer (Biorad, 1610737) for 5 minutes to elute the proteins ...
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bioRxiv - Neuroscience 2024Quote: ... This was followed by overnight incubation at 4°C with primary antibody against CD13 (Bio-Rad, MCA2183) at a 1:100 dilution in the blocking solution ...
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bioRxiv - Cancer Biology 2024Quote: ... boiled at 95°C for 5 minutes and processed with Mini-Protean Gel Electrophoresis Systems (Bio-Rad) using Bis-Tris 4–20% gradient pre-cast gels (Biorad).
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bioRxiv - Cancer Biology 2024Quote: ... boiled at 95°C for 10 minutes and processed with Mini-Protean Gel Electrophoresis Systems (Bio-Rad) using Bis-Tris 4–20% gradient pre-cast gels (Biorad).
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bioRxiv - Cell Biology 2024Quote: ... 30 s off) and boiled 5 min at 95°C in 1x Laemmli sample buffer (Bio-Rad), containing 5% □-Mercaptoethanol (v/v) ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were then incubated at 4°C with primary antibodies diluted in EveryBlot Blocking Buffer (Bio-Rad) overnight ...
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bioRxiv - Plant Biology 2021Quote: ... After washing 3 times with TBST the chemiluminescent was conducted by adding Clarity Western ECL Substrate (Biorad, #1705061) for 2 min and detected by ChemiDoc imaging system (Biorad) ...
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bioRxiv - Microbiology 2022Quote: ... Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was detected with 1:1,000 anti-GAPDH hFAB™ Rhodamine antibody (Bio-Rad). The CA levels from cells were normalized relative to GAPDH levels ...
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bioRxiv - Neuroscience 2022Quote: ... 40 μg of samples 3 were mixed with 2× laemmli buffer + 5% 2-mercaptoethanol (BioRad Laboratories, Hercules, CA). Samples were then briefly vortexed and centrifuged ...
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bioRxiv - Cancer Biology 2021Quote: ... and 3 μg of protein was separated on 4% to 20% precast polyacrylamide gels (Bio-Rad, cat # 4561095) for detection of histone H3 andH3K27me3 ...
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bioRxiv - Biochemistry 2020Quote: ... The purified MCU-EMRE subcomplex in DDM was then reconstituted into nanodisc by incubating with Msp1 protein, lipids (POPC:POPE:POPG, 3:1:1 molar ratio) and BioBeads (BioRad) at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... Membranes were again washed 3 times in PBST before being incubated with Clarity™ ECL reagents (Bio-Rad) or SuperSignal™ West Femto Maximum Sensitivity Substrate and visualized (see S11 Fig for full length immunoblots).