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Citations for Bio-Rad :
6751 - 6800 of 7302 citations for Superoxide Anion Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2024Quote: ... Hrd1 and GAPDH was quantified through one-step RT-qPCR using iTaq Universal SYBR green one-step kit (Bio-Rad) with the corresponding primiers (Table S5 ...
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bioRxiv - Microbiology 2024Quote: TNF-α and IL-10 were quantified in the collected supernatants using Bio-Plex Pro Human Luminex kit (Bio-Rad) according to the manufacturer’s instructions.
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bioRxiv - Genetics 2024Quote: ... and reverse transcribed by Maxima first strand cDNA synthesis kit (Thermo Fischer) using Bio-Rad CFX Maestro 1.1 software (Bio-Rad). The primers used are listed in Supplementary table 6.
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bioRxiv - Molecular Biology 2024Quote: ... Gene-specific cDNA synthesis was carried out with 2 µg of mtRNA using the iScript Select cDNA Synthesis Kit (BioRad) and oligo 2278 (see Supplementary Table ST2) ...
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bioRxiv - Genomics 2024Quote: ... 500 ng of RNA was further reverse transcribed to cDNA using the iScript cDNA synthesis kit (Bio-Rad, CA, USA). Semi-quantitative PCR was carried out using specific primers for the following genes ...
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bioRxiv - Systems Biology 2024Quote: ... 1 µg of total RNA were processed using the iScript™ gDNA Clear cDNA Synthesis Kit (Bio-Rad; www.bio-rad.com), to achieve genomic DNA digestion and first-strand cDNA synthesis ...
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bioRxiv - Neuroscience 2024Quote: ... and the RNA was reverse transcribed into cDNA using the iScript™ gDNA Clear cDNA Synthesis Kit (1725035, Bio-Rad), as per the manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2024Quote: ... 500 ng of total RNA was converted to cDNA using iScript advanced cDNA synthesis kit for RT-qPCR (Cat# 1725037, Biorad) according to the instructions of the manufacturer.
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bioRxiv - Cell Biology 2024Quote: ... submerged with ECL solution (ECL Prime Kit; Amersham, UK) and chemiluminescence signals were detected using the ChemiDoc system from BioRad. The following antibodies were used ...
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bioRxiv - Immunology 2024Quote: ... cDNA was made from equivalent starting amounts for each sample and synthesized using the iScript cDNA Synthesis Kit (Bio-Rad). Total cDNA was diluted 1:10 in water and amplified by real-time polymerase chain reaction using a BioRad CFX-96 iCycler and SYBER Green Supermix ...
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bioRxiv - Plant Biology 2020Quote: ... One microgram of total RNA was reverse transcribed to complementary DNA (cDNA) using the iScript cDNA Synthesis Kit (Biorad, Hercules, California) in a 20 μL reaction volume ...
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bioRxiv - Cell Biology 2020Quote: ... and detected using the antibodies listed in Supplementary File 3 with either SuperSignal West Dura or SuperSignal Femto kits (Pierce, Waltham, MA) and a ChemiDoc XRS imaging system (Bio-Rad). Band intensities were quantified using ImageLab software (Bio-Rad ...
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bioRxiv - Cell Biology 2019Quote: RNA was extracted from 40 w1118 or de2f1b 80-85 hr AEL salivary glands using the Aurum Total RNA Mini Kit (Bio-Rad) with treatment of DNase I to remove genomic DNA contamination ...
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bioRxiv - Genetics 2021Quote: ... qPCR was carried out using BioMaster HS-qPCR SYBR Blue (2×) reagent kit (Biolabmix; https://biolabmix.ru/en/) and CFX96™ Real-Time PCR Detection System (Bio-Rad). We used the following thermal cycling conditions ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 µg of total RNA and oligo (dT) primers were reverse transcribed using the iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad). RT-PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... was performed as a two-step RT-qPCR by first synthesizing oligo(dT) primed cDNA using the iScript™ Select cDNA Synthesis Kit (Biorad), then running ddPCR via the standard EvaGreen program on a Biorad QX200 ddPCR system and analyzing in Quantasoft software (see Table S2 for primer sequences).
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bioRxiv - Cell Biology 2022Quote: ... membranes were washed again 3 times 10min in 1X TBS-T and developed using the Clarify Western ECL substrate kit (Bio-Rad) or SuperSignal West Femto (ThermoFisher Scientific) ...
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bioRxiv - Systems Biology 2022Quote: ... Drugs were then added to the plates and protein was harvested after 2 hours using the Bio-Plex Pro Cell Signaling Reagent Kit (BioRad 171304006M) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... and TNF-α were quantified using real-time PCR and the SsoAdvanced Universal SYBR Green Supermix Kit (BIO-RAD, Hercules, CA). β-actin was used as an internal control ...
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bioRxiv - Neuroscience 2021Quote: ... analysis was performed through reverse transcription on 0.5-1 μg of total RNA using the iScript cDNA synthesis kit according to the manufacturer’s instructions (Bio-Rad, Hercules, CA). The cDNA was diluted 1:20 in nuclease-free water ...
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bioRxiv - Neuroscience 2021Quote: ... Total RNA (280 ng per sample) was used to generate cDNA using the iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad). The cDNA was used as a template for qRT-PCR with SsoFast EvaGreen Supermix (Bio-Rad) ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 μL of DNase-treated samples were reverse transcribed in a final volume of 10 μL using the iScript cDNA synthesis kit (Bio-Rad) following the manufacturer’s protocol and diluted 5-fold in nuclease-free water before qPCR ...
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bioRxiv - Molecular Biology 2021Quote: ... Quantitative real-time PCR expression analysis of selected candidates was performed using cDNAs produced from the iScript cDNA Synthesis Kit (Bio-Rad) in a LightCycler 480 system (Roche ...
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bioRxiv - Immunology 2020Quote: ... 18.10 is a murine IgG1 anti-human HVEM mAb and was produced as ascites and purified by protein A binding and elution with the Affi-gel Protein A MAPS II Kit (Bio-rad). The mouse IgG1 isotype control (clone MOPC-21 clone) ...
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bioRxiv - Bioengineering 2022Quote: ... cDNA was produced using the High Capacity RNA-to-cDNA Kit (Applied Biosystem) and the cDNA was pre-amplified with SsoAdvanced PreAmp Supermix (Bio-Rad) using primers from Integrated DNA Technologies or Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... 0.5 μg of total RNA sample (100 ng/μl) was retrotranscribed in stable cDNA with iScriptTM Advanced cDNA Synthesis Kit (Bio-Rad). Genes of interest were amplified with Itaq UniversSYBR Green (Bio-Rad) ...
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bioRxiv - Cell Biology 2022Quote: ... Up to 5 μg of total RNA was reverse transcribed into cDNA using iScriptTM Advanced cDNA Synthesis Kit for RT-qPCR (Bio-Rad). Quantitative real time-PCR was performed using GoTaq® qPCR Master Mix (Promega ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 ng of total RNA was used to generate cDNA via the iScript Advance cDNA Kit for RT-qPCR (BioRAD #1725037), for each sample including no reverse transcriptase and no template controls following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 ng of total RNA was used to generate cDNA via the iScript Advance cDNA Kit for RT-qPCR (BioRAD #1725037), for each sample including no reverse transcriptase and no template controls following the manufacturer’s protocol ...
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Characterization of pyruvate dehydrogenase complex E1 alpha and beta subunits of Mycoplasma synoviaebioRxiv - Microbiology 2019Quote: Membrane and cytosolic proteins fractions from MS were extracted using ReadyPrep™ Protein Extraction Kit (Membrane I) (Bio-Rad, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... The cDNA samples were used in a qRT-PCR experiment employing the iQ SYBR Green Supermix Kit (Bio-Rad, Munich, Germany). PCR was conducted in a CFX96 RT-PCR Detection System (Bio-Rad ...
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bioRxiv - Plant Biology 2019Quote: Agarose plug embedded nuclei were Proteinase K treated for two days followed by RNAse treatment (Biorad CHEF Genomic DNA Plug Kit). DNA was recovered from agarose plugs according to IrysPrep™ Plug Lysis Long DNA Isolation guidelines (BioNano Genomics) ...
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bioRxiv - Immunology 2020Quote: ... A 1 µg sample of total RNA was reverse transcribed to complementary DNA using the iScript cDNA Synthesis Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... An amount of 500-1000 ng of purified total RNA was then subjected to reverse transcription using the iScript cDNA synthesis kit (Bio-Rad), following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... 800 ng of purified RNA was used as a template to generate cDNA with the iScript cDNA Synthesis Kit (Bio-Rad). End-point PCR was then conducted using primer sets EH13/EH14 and EH1/EH2 ...
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bioRxiv - Physiology 2019Quote: ... Antennal cDNA was synthesized from 1 µg of antennal total RNA from each species using iScript cDNA synthesis kit (Bio-Rad) according to the manufacturer’s instructions (Clontech ...
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bioRxiv - Microbiology 2019Quote: ... 39) and 1 µg of total RNA was reverse transcribed to cDNA using the iScript Select cDNA Synthesis Kit (Bio-Rad) with random hexamers ...
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Dual functionality of the TasA amyloid protein in Bacillus physiology and fitness on the phylloplanebioRxiv - Microbiology 2019Quote: Quantitative real-time (qRT)-PCR was performed using the iCycler-iQ system and the iQ SYBR Green Supermix Kit from Bio-Rad. The primer pairs used to amplify the target genes were designed using the Primer3 software (http://bioinfo.ut.ee/primer3/ ...
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bioRxiv - Plant Biology 2019Quote: ... and the Takyon No ROX SYBR 2X MasterMix blue dTTP qPCR Kit (both Eurogentech) for quantitative real time PCR using a CFX96 detection system (Bio-Rad). The previously described reference genes Protein Phosphatase 2A (PP2A ...
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bioRxiv - Cell Biology 2019Quote: ... RNA concentration and quality were determined using Nanodrop (PeqLab) and cDNA was synthesized with the iScript cDNA synthesis kit (Bio-Rad) following manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... We then performed quantitative PCR (qPCR) using 2 ng of cDNA for each reaction using the iTaq Universal SYBR Green Supermix kit (Bio-Rad) on a Bio-Rad CFX384 thermocycler ...
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Transcriptomic and morphological response of SIM-A9 mouse microglia to carbon nanotube neuro-sensorsbioRxiv - Bioengineering 2020Quote: Total RNA from SIM-A9 cells with varying treatment conditions was reverse transcribed to cDNA libraries using the iScript cDNA Synthesis Kit (Bio-Rad) with a 1 μg RNA input ...
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bioRxiv - Physiology 2019Quote: ... The cDNA synthesis was performed with 1μg of total RNA using the iScript cDNA synthesis kit (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Bioengineering 2021Quote: ... The HRP conjugated secondary antibodies were visualized by using an enhanced chemiluminescence detection kit and ChemiDoc Touch Imaging System (Image Lab, Bio-Rad).
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bioRxiv - Evolutionary Biology 2020Quote: ... DNase-treated RNA was used to perform cDNA synthesis in a final volume of 20 μl using the iScript cDNA synthesis kit (Biorad, USA). The reaction was performed at 46°C for 20 min ...
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bioRxiv - Cancer Biology 2020Quote: ... The chemiluminescence of the membranes was developed using Super Signal Femto Substrate Kit (Pierce) and developed using ChemiDoc Touch Imager (Bio-Rad). Primary antibodies and conditions used are as follows ...
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bioRxiv - Genomics 2021Quote: ... qRT-PCR was performed using the TaqMan Fast Advanced Cells-to-CT kit with the FX96 Real-Time PCR Detection System (Bio-Rad) with the TaqMan probes listed in table S15 ...
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bioRxiv - Immunology 2021Quote: ... were detected by staining membranes with anti-mouse IFNγ biotin (1mg/mL) (R46A2) followed by streptavidin-Alkaline Phosphatase (1mg/mL) and development with AP conjugate substrate kit (BioRad, UK). Spots were enumerated using an AID ELISpot reader and software (AID).
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bioRxiv - Systems Biology 2021Quote: ... Transfer was done into PVDF membranes using a Trans-Blot Turbo Transfer System and Trans-Blot Turbo RTA Transfer Kit (BioRad, #1704273). After 1 h of blocking in 5% milk ...
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bioRxiv - Neuroscience 2020Quote: ... Five hundred ng of purified RNA were used for cDNA synthesis using an iScript cDNA synthesis kit (Bio-Rad, Irvine, CA), and real-time quantitative PCR (qPCR ...