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Citations for Bio-Rad :
6701 - 6750 of 7302 citations for Superoxide Anion Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Complementary DNA (cDNA) was created for each sample from equals amounts of total RNA using the iScript cDNA synthesis kit (1708890 BioRad). Real-time PCR was performed from cDNA on a StepOnePlus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... qPCR was performed using the SYBR Green Supermix Kit (Vazyme) on the CFX96 Real-Time PCR System (Bio-Rad, USA). The expression of target genes was normalized to expression of Gapdh ...
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bioRxiv - Microbiology 2023Quote: ... first-strand cDNA synthesis was prepared from 200 ng of total RNA using the iScript cDNA Synthesis Kit (Bio-Rad), according to the manufacturer’s instructions ...
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ANGPTL8 R59W variant influences inflammation through modulating NF-κB pathway under TNFα stimulationbioRxiv - Biochemistry 2023Quote: ... interleukins and ghrelin were measured using Biolpex multiplex kits (cat# M50-0KCAFOY and cat # 171-A4S01M, Bio-Rad, CA, USA). Standards with known concentrations of specific analytes were reconstituted and diluted as instructed in the manual ...
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bioRxiv - Cancer Biology 2023Quote: ... tagmentation and droplet library preparation were performed following the SureCell ATAC-Seq Library Prep Kit User Guide (17004620, Bio-Rad). Harvested cells and tagmentation related buffers were chilled on ice ...
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bioRxiv - Molecular Biology 2023Quote: ... We then utilized 1 µg of the total RNA for the SEQuoia Express Standard RNA Library Prep Kit (Bio-Rad). rRNA depletion was performed with riboPOOL for D ...
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bioRxiv - Microbiology 2023Quote: ... Separated proteins were then transferred to a nitrocellulose membrane using a Trans-Blot Turbo RTA Mini 0.2 μm Nitrocellulose Transfer Kit and Trans-Blot Turbo system (Bio-Rad). The membrane was cut to separate the regions above and below approximately 70 kDa ...
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bioRxiv - Cell Biology 2023Quote: ... The secondary antibody was washed away 5× with TBS-T and developed with an AP Conjugate Substrate Kit (Bio-Rad). Spots were quantified using a Nikon ELISPOT system and NIS-Elements AR software (v ...
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bioRxiv - Physiology 2023Quote: ... 500 ng of total RNA was reverse transcribed using the iScript cDNA Synthesis Kit (Bio-Rad #1708891, Hercules, CA, USA) per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... A 1.0 μg of total RNA was used for the cDNA synthesis using the iScript cDNA Synthesis kit (Bio-Rad). The qRT-PCR analysis was performed using CFX 384 Touch RT-PCR detection system with a reaction mixture of 2.0 μl of primer mix (2.0 μM from each F & R primer) ...
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bioRxiv - Molecular Biology 2023Quote: ... The following qRT-PCR thermocycling protocol was used with the iTaqTM universal SYBR® Green One-Step kit (Bio-Rad): 95 °C for 5 min ...
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bioRxiv - Developmental Biology 2023Quote: Total RNA was extracted from sorted cardiac cells or from cardiac tissues (atria or ventricle) using the Aurum total RNA fatty and fibrous tissues kit (Biorad) and following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... A part of the samples was then analyzed by a BioRad Fish Barcoding Kit (Bio-Rad Laboratories Inc., Hercules, CA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: cDNA was prepared after extracting total RNA from young adult worms or cell extracts using the iScript cDNA Synthesis Kit (BioRad) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: 500ng of RNA/sample was used to make cDNA using iScript cDNA synthesis kit according to manufacturer’s protocol (Bio-Rad). cDNA abundance was measured using 300nM of indicated primers and a iQ5 qPCR system (Bio-Rad).
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bioRxiv - Molecular Biology 2022Quote: 500-1000 ng of RNA were used for the retrotranscription reaction using iScript cDNA Synthesis Kit (BioRad, CA, USA, #1708890). Expression values were determined by q-PCR using Sybr Green (iTaq SYBR Green Supermix ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was synthesized from total purified RNA (1 μg) from each sample by using iScript cDNA synthesis kit (Bio-Rad) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... After incubation with HRP-coupled secondary antibody diluted in saturation buffer (1 h, room temperature), membranes were revelated using ECL kit (Pierce, 34076) on Chemidoc XRS+ imager (Biorad). Images were processed and analyzed using ImageJ software ...
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bioRxiv - Cancer Biology 2022Quote: ... A total of one μg of total RNA was used for reverse transcription with iScript cDNA synthesis kit (Bio-Rad). Real-time PCR was performed using SYBR Green PCR Master Mix (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... One μg of RNA was used to perform the reverse transcriptase reaction with iScript TM cDNA Synthesis Kit (Bio-Rad). Changes in mRNA levels of the target genes were determined by relative RT-qPCR with a CFX96TM Real‒Time PCR Detection System (Bio-Rad ...
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bioRxiv - Physiology 2023Quote: ... ∼300ng of purified RNA was revers transcribed into cDNA in the 20 μL using iScript cDNA synthesis kit (Bio-Rad). 2 μL of this reaction was used for subsequent qPCR reactions ...
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bioRxiv - Cancer Biology 2023Quote: ... The RNA concentration was quantified by spectrophotometry and used to prepare cDNA using the iScript cDNA Synthesis Kit (Bio-RAD), followed by the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Real-time RT-qPCR was performed using the AB StepOnePlus Detection System and the one-step kit RT-PCR Master Mix Reagents (#64471423, Biorad). Reaction mixtures were prepared according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... leaf tissue was ground in liquid N2 and total RNA was extracted using the Aurum Total RNA Mini Kit (BioRad) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... 800 ng of total RNA were used to perform the RT step using the iScript cDNA synthesis kit (Bio-Rad) in a total volume of 20 μL ...
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bioRxiv - Physiology 2023Quote: ... Secondary antibodies conjugated with alkaline phosphatase were used with an ImmunStar™-Alkaline Phosphatase (AP) Western Chemiluminescent Kit (Bio-Rad) for signal development ...
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bioRxiv - Physiology 2024Quote: ... 450 ng of mRNA from Quad (Fig. 1A) or PLA (Fig. 2C, Myc only) muscle were reverse transcribed using the iScript cDNA Synthesis kit (Biorad). Next ...
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bioRxiv - Microbiology 2023Quote: HIV-1/N and M strains were quantified by targeting the long terminal repeat (LTR) region using GENERIC HIV Charge virale kit (Biocentric) performed on CFX96 Deep-Well (Biorad) with the followed program RT 10 min at 50°C and 5 min at 95°C ...
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bioRxiv - Microbiology 2023Quote: ... The cDNA used for ddPCR was transcribed using the iScriptTM Advanced cDNA Synthesis Kit for RT-qPCR (Biorad, Munich, Germany).
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bioRxiv - Microbiology 2023Quote: ... Proteins were transferred to PVDF membranes using a Trans-Blot Turbo RTA Mini 0.2 µm PVDF Transfer Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... RNA from vehicle (DMSO)-treated and 1 μM CHIR-treated cells was extracted using an Aurum Total RNA Kit (BioRad) and stored at -80°C ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was prepared using iScriptTM cDNA synthesis kit and qRT-PCR was performed using a CFX96 Touch Real-Time PCR Detection System (BioRad). Data were analyzed using the following equations ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA (1μg) was reverse transcribed into complementary DNA using an iScript Reverse Transcription Supermix kit (Bio-Rad, Hercules, CA, USA). The relative levels of gene transcripts compared with the control gene 18S were determined by quantitative real-time PCR using SYBR Green PCR Supermix (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... TX) and 750ng of RNA was reverse transcribed to generate cDNAs using iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA). Relative transcript levels were analyzed by real-time qPCR using SYBR Green (SsoAdvanced Universal SYBR Green Supermix ...
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bioRxiv - Cancer Biology 2023Quote: ... and the product band was excised and extracted using the Freeze and Squeeze Kit according to the manufacturer’s instructions (Bio-Rad). Gel-purified amplicons were quantified with a dsDNA HS Assay kit on a Qubit fluorometer (ThermoFisher Scientific) ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... aliquots constituting 250 ng of RNA were diluted with nuclease-free water to a volume of 15 µL and processed using the iScriptTM cDNA Synthesis Kit (BioRad Laboratories Inc. ...
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bioRxiv - Genomics 2023Quote: ... Two μg of total RNA each was used for cDNA synthesis using iScript™ cDNA Synthesis Kit (Bio-Rad, USA). Oligonucleotide primers used for RT-PCR for each gene are listed in Supplementary Table S4 ...
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bioRxiv - Physiology 2023Quote: ... 500 ng RNA was reverse transcribed using HiScript III 1st Strand cDNA Synthesis Kit followed by qPCR reactions using SYBR Green master mix and analyzed by Biorad CFX Touch Real-Time PCR Detection System (SIA-PCR008) ...
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bioRxiv - Biophysics 2023Quote: ... proteins were transferred to PVDF membranes using a Trans-Blot Turbo RTA Mini 0.2 μm PVDF Transfer Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Membrane strips were finally washed 6x for 3mins in 1X TBST before being developed with the ECL Clarity Kit (BioRad) and imaged on ChemiDoc Imaging system (BioRad) ...
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bioRxiv - Neuroscience 2023Quote: ... High integrity RNA (RIN > 7) was used to synthesize cDNA with iScript™ gDNA Clear cDNA Synthesis Kit (Bio-Rad). PCR primers for human B-ACTIN ...
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bioRxiv - Microbiology 2024Quote: TNF-α and IL-10 were quantified in the collected supernatants using Bio-Plex Pro Human Luminex kit (Bio-Rad) according to the manufacturer’s instructions.
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bioRxiv - Genetics 2024Quote: ... and reverse transcribed by Maxima first strand cDNA synthesis kit (Thermo Fischer) using Bio-Rad CFX Maestro 1.1 software (Bio-Rad). The primers used are listed in Supplementary table 6.
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bioRxiv - Cell Biology 2024Quote: ... submerged with ECL solution (ECL Prime Kit; Amersham, UK) and chemiluminescence signals were detected using the ChemiDoc system from BioRad. The following antibodies were used ...
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bioRxiv - Molecular Biology 2024Quote: HepG2 protein lysate was separated by SDS-Page (TGX Stain-FreeTM FastCastTM Acrylamide Kit, Bio-Rad Laboratories, Inc., Hercules, US) and blotted on nitrocellulose membranes (Trans-Blot® TurboTM ...
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bioRxiv - Plant Biology 2024Quote: ... cDNA was then synthesized from 1 µg total RNA (measured by spectrophotometer) with the iScriptTM cDNA synthesis kit (Bio-RAD) according to the company’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... were transferred to a 0.45μM nitrocellulose membrane (Trans-Blot Turbo RTA Transfer kit nitrocellulose midi #1704271) using Trans-Blot Turbo Transfer System (Bio-Rad #1704150 ...
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bioRxiv - Molecular Biology 2024Quote: ... The washed membrane was detected using the Clarity Western ECL kit following manufacturer protocol and imaged using a ChemiDoc XRS+ apparatus (BioRad). Pixel density for each dot was quantified using the volume tool in ImageLabTM software (BioRad).
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bioRxiv - Pathology 2024Quote: ... We used 200ng of total RNA to synthesize single-stranded cDNA using iScript cDNA synthesis kit based on manufacturer’s directions (Bio-Rad). cDNA was then used for qRT-PCR analysis using SYBR Green qPCR master mix (Bio-Rad ...
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bioRxiv - Plant Biology 2024Quote: ... Detection was performed using ECL Advance Western Blotting Detection Kit (Amersham) and the chemiluminescence was detected using an ChemiDoc Imaging System (BioRad).