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Citations for Bio-Rad :
6601 - 6650 of 7302 citations for Superoxide Anion Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... we synthesized first-strand cDNA from cellular RNA isolated (100ng) from each sorted sample using iScriptTM cDNA synthesis kit (BioRad). Each rtPCR reaction included 25°C for 5 mins ...
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bioRxiv - Neuroscience 2022Quote: ... an equal amount (500 ng) of RNA was reverse transcribed to cDNA using iScript reverse-transcription kit (Bio-rad, 1708841). Quantitative real time PCR (45 cycles – LightCycler480 software ...
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bioRxiv - Microbiology 2022Quote: ... Viral copy numbers were assessed via digital-droplet PCR (ddPCR) using SARS-CoV-2 ddPCR kit (#12013743) from Bio-Rad with N1 and N2 primers and probes targeting the nucleocapsid genes ...
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bioRxiv - Microbiology 2022Quote: HCV RNA copies were determined by Taqman RT-qPCR using a Quanta BioSciences qScript XLT One-Step RT-qPCR kit according to the manufacture’s instruction (Bio-Rad). GLT1_probe was used to detect for GLT1 RNA ...
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bioRxiv - Cell Biology 2022Quote: ... following the manufactures instructions and cDNA was synthesized from 1μg of total RNA using iScript cDNA Synthesis Kit (Bio-Rad). QPCR was performed on a CFX96 real-time PCR thermocycler (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: ... Up to 1 µg of total RNA was reverse transcribed into cDNA using the iScript cDNA Synthesis Kit (Bio-Rad) or gDNA Clear iScript cDNA Synthesis Kit for patient-derived tumor cells (Bio-Rad ...
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bioRxiv - Genomics 2022Quote: ... The blots were developed with an enhanced chemiluminescence kit (Thermo Fischer Scientific) and signals acquired by a ChemiDoc instrument (BioRad). All primary histone variant-specific and H3 marks-specific antibodies were used at 1 µg/ml dilution ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were transferred onto a nitrocellulose membrane using Trans-Blot® Turbo™ RTA Midi Nitrocellulose Transfer Kit (Bio-Rad) in the Trans-Blot® Turbo™ Transfer System (Bio-Rad) ...
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bioRxiv - Neuroscience 2021Quote: ... Equal amounts of whole protein from each sample was subjected to SDS-PAGE using the TGX Stain-Free FastCast Acrylamide kit (BioRad). Protein bands were transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Microbiology 2020Quote: Viral RNA was quantified via a one-step qRT-PCR using the iTaq universal probes one-step kit (Bio-Rad) per the manufacturer’s instructions for a 20µL reaction ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA was synthesized from 1 μg of purified RNA using the iScript cDNA synthesis kit (Cat. no. 1708891, Bio-Rad). Quantitative real-time PCR assays were performed using the iQ SYBR Green Supermix (Cat ...
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bioRxiv - Microbiology 2021Quote: ... Blots were developed using the SuperSignal West Pico PLUS chemiluminescent substrate kit (Thermo) and imaged using the ChemiDoc MP Imaging System (BioRad).
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bioRxiv - Plant Biology 2021Quote: ... Quantification of RNA sample quality and concentration was performed using the Experion ‘StdSens’ kit (Bio-Rad Laboratories, Inc., Hercules, CA). Stranded RNA-Seq libraries were created and quantified by qPCR (2×76 bp or 2×151 bp ...
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bioRxiv - Microbiology 2021Quote: ... Distinct MagPlex microsphere sets (Luminex Corp) were respectively coupled to viral antigens using the amine coupling kit (Bio-Rad Laboratories) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 µg of RNA from each sample was reverse-transcribed in 40 µl using iScript™ cDNA Synthesis Kit (BioRad).
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bioRxiv - Plant Biology 2021Quote: ... proteins from SDS-PAGE gels were transferred onto nitrocellulose membranes using the Transblot Turbo RTA Midi Transfer kit (BioRad, USA). Mouse anti-α-tubulin antibody (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... was used to determine RNA concentrations and cDNA synthesis was performed using the iScript Advanced cDNA synthesis kit (Bio-Rad) using a mix containing 200 ng of RNA ...
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bioRxiv - Biochemistry 2021Quote: ... 500 ng of DNaseI-treated RNA was used for cDNA synthesis using the iScript cDNA synthesis kit (Bio-Rad, USA). Quantitative RT-PCR was performed using SsoAdvanced SYBR green supermix (Bio-Rad ...
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bioRxiv - Neuroscience 2022Quote: ... the membrane was then treated for 45 min at room temperature with the appropriated HRP-conjugated secondary antibody: Proteins were visualized using an ECL+ detection kit (Amersham) and imaged using a Chemidoc Touch Imaging system (Biorad). Densitometry was analyzed using the ImageLab software.
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bioRxiv - Genetics 2022Quote: ... 500 ng (sorted cells) to 1 μg (unsorted cells) of total RNA was used for reverse transcription using iScript cDNA synthesis kit (Biorad). The cDNA product was diluted at 1:20 and 2 μl of diluted cDNA was used for real time PCR analysis using iQ SYBR green supermix (Biorad) ...
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bioRxiv - Cancer Biology 2022Quote: ... About 500 ng of total RNA was used for cDNA synthesis (iScript cDNA synthesis kit, Bio-Rad, Hercules, California, USA). cDNA was diluted 1:5 before use in PCR reactions ...
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bioRxiv - Molecular Biology 2022Quote: ... 500ng for males per sample) was subsequently reverse-transcribed to cDNA using iScript cDNA Synthesis Kit (Bio-Rad Laboratories, Inc.)
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bioRxiv - Molecular Biology 2019Quote: ... The membranes were then washed with TBST buffer for three times and detected using AP detection kit (Bio-Rad, USA). Protein bands were visualized using Gel DocTM XR+ System (Bio-Rad ...
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bioRxiv - Neuroscience 2019Quote: ... Reverse transcription was performed on 0.5-1μg of total RNA using the iScript cDNA synthesis kit according to the manufacturer’s instructions (Bio-Rad, Hercules, CA). The cDNA was diluted 1:10 in nuclease-free water ...
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bioRxiv - Developmental Biology 2019Quote: ... the samples were run in a 12% SDS-polyacrylamide gel and Western blots were developed using an ECL Western blotting kit (Biorad). Rabbit anti-countin antibodies were used at 1 ...
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bioRxiv - Genomics 2019Quote: Two hundred clonal adult male worms were used to prepare high molecular weight genomic DNA using CHEF Genomic DNA Plug Kits (BioRad). The DNA was quantified on a FEMTO Pulse® ...
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bioRxiv - Genetics 2020Quote: ... Total RNA (12.5 ng) was reverse transcribed and amplified with the iTaq Universal SYBR Green One-Step Kit (Bio-Rad). RUNX1 and ACTB were amplified in parallel in a CFX96 Touch Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... was used at a dilution of 1:10000 for overnight at 4°C and probed for protein using ECL chemiluminescence kit (BioRad). HPLC purified protein was taken as control ...
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bioRxiv - Cell Biology 2019Quote: ... Complementary DNA (cDNA) was made by converting at least 100 ng RNA with the iScript cDNA synthesis kit (Bio-Rad). Quantitative PCR (qPCR ...
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bioRxiv - Cancer Biology 2021Quote: Small pieces of dissected frozen pancreatic tumor tissues were collected and lysed (Bio-Plex Cell Lysis Kit, BioRad, USA; 171304011), followed by a repeated procedure of vortexing ...
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bioRxiv - Developmental Biology 2020Quote: ... cDNA was synthesized on 1μg of isolated RNA by reverse transcription with random hexamer primers using the iScript Select cDNA synthesis kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... the gels were washed in ddH2O and then silver stained following the manufacturers instruction (Silver Stain Plus Kit, 1610449, Biorad).
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bioRxiv - Cell Biology 2021Quote: ... Gene expression analysis was conducted with 50 ng RNA using iTaq Universal One-Step RT-qPCR Kit (Bio-rad, 1725140). The reaction was carried out according to the manufacturer’s instructions using CFX Connect Real-Time PCR Detection System (Bio-rad ...
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bioRxiv - Neuroscience 2019Quote: ... Equal amounts of RNA (1.5 µg per sample) were reverse transcribed using an iScript Advanced cDNA synthesis kit (Bio-Rad). Resulting cDNA was diluted 1:50 in ultrapure water ...
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bioRxiv - Plant Biology 2019Quote: ... RNA quality was assessed with an Experion Automated Electrophoresis Station using the Experion RNA StdSens analysis kit (BioRad, Hercules, CA) and yield was quantified with an ND-1000 spectrophotometer (Nanodrop ...
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bioRxiv - Neuroscience 2019Quote: ... conjugated secondary antibody (1:10 000) was subsequently applied and visualized by reaction with chemiluminesence reagent (Clarity Western Blotting Substrates Kit, BioRad) on radiography film (Carestream Blue X-ray film).
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bioRxiv - Physiology 2021Quote: RNA was isolated using a Macherey-Nagel NucleoSpin RNA kit and cDNA was synthesized using iScript RT Supermix (Bio-Rad). Samples were run on a Bio-Rad CFX96 Touch to assess mRNA levels of BNP ...
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bioRxiv - Biochemistry 2021Quote: The 100µl plasma was inactivated by heating at 56°C for 30 min 10 .60µl plasma was used for albumin and globulin depletion using an Aurum serum mini kit (BioRad, USA). The six depleted plasma samples were used for SWATH-MS analysis ...
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bioRxiv - Physiology 2020Quote: ... and circulating factor concentrations were measured using the following kits according to the respective manufacturer’s protocols: Bio-Plex protein array system (Biorad, UK) with one multiplex panel was used to measure total Glucagon-like peptide-1 (GLP-1) ...
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bioRxiv - Cancer Biology 2021Quote: ... 3 μg of total RNA was used in the reverse transcription reaction using the iScript cDNA synthesis kit (Bio-Rad). Quantitative PCR amplification was performed on the Prism 7900 Sequence Detection System (Applied Biosystems ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were then transferred to PVDF membranes using Trans-Blot Turbo RTA Mini 0.45 mM LF PVDF Transfer Kit (Bio-Rad), and blotted with anti-HA antibody ...
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bioRxiv - Synthetic Biology 2021Quote: ... Protein transfer to the PVDF membrane was performed following manufacturer’s instructions for the membrane kit (Trans-Blot Turbo Midi 0.2 μm PVDF, 1704157, BioRad, Hercules, CA) and the transfer apparatus (Trans-Blot® Turbo™ Transfer System ...
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bioRxiv - Genetics 2021Quote: ... Immunolabelling was visualized using chemiluminescence kits (SuperSignal West Pico PLUS Chemiluminescent Substrate; ThermoScientific) on a Chemidoc MP Imaging System (Biorad) and analyzed with the ImageLab Software (version 5.2 ...
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bioRxiv - Neuroscience 2021Quote: ... A qScript cDNA Synthesis kit (QuantaBio, Beverly, MA, USA) and T100 Thermal Cycler (Bio-Rad Laboratories, Inc., Hercules, CA, USA) were used to generate the cDNA ...
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bioRxiv - Developmental Biology 2020Quote: ... mRNA was used for RNA-seq analysis or prepared for qRT-PCR using the iScript cDNA Synthesis Kit (Bio-Rad). cDNA was used as template in SYBR green qPCR supermix (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: qPCR was performed using the iTaq universal SYBRGreen® kit following the manufacturer protocol (Bio-Rad Laboratories Inc, Hercules, CA) on an Agilent Mx3005P qPCR Systems (Agilent Technologies ...
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bioRxiv - Immunology 2020Quote: Thymi isolated from Foxp3hCD2 x Rag1GFP reporter mice were homogenized and lysed using Bio-Plex Cell Lysis Kit (BioRad Laboratories), and the protein content of the thymic lysates was measured using Pierce™ BCA Protein Assay Kit (Thermo Scientific) ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA with a RIN value of at least 8 was used for cDNA synthesis using the iScript cDNA synthesis kit (BioRad) according to manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... 100-200 ng of RNA was used as input for cDNA synthesis using the iScript cDNA synthesis kit (Bio-Rad) on an Arktik thermal cycler (Thermo scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes went through a final TBST wash and then visualized on the ChemiDoc XRS+ using the Clarity Western ECL kit (BioRad).