Labshake search
Citations for Bio-Rad :
7001 - 7050 of 7302 citations for Superoxide Anion Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: Total RNA was extracted from cells using GeneJet RNA Purification kit (Thermo Fischer) and cDNA was synthesized using iScript Reverse Transcription Supermix (Bio-Rad), as previously described (27) ...
-
bioRxiv - Microbiology 2024Quote: ... the blots were washed four times again by PBST and visualized using an Omni-ECL Femto Light Chemiluminescence Kit (EpiZyme, SQ201) through a ChemiDoc MP Imaging System (Bio-Rad). To examine the S glycoprotein packaging efficiency ...
-
bioRxiv - Plant Biology 2024Quote: ... 5 μl supernatant was loaded onto 10% SDS polyacrylamide gels and the proteins were transferred onto PVDF membranes using the Trans-Blot Turbo RTA Transfer Kit (1704275, Bio-rad). Membranes were blocked in PBS-T (tablets 524650-1EA ...
-
bioRxiv - Plant Biology 2024Quote: ... graminicola genomic DNA was quantified by quantitative PCR using Biozym Blue S’Green qPCR Kit (Biozym Scientific, Germany) in a CFX RT-PCR detection system (Bio-Rad, USA) and specific primers for the histone 3 (CgH3 ...
-
bioRxiv - Plant Biology 2024Quote: ... The rRNA depleted was used immediately to prepare the metatranscriptome libraries using the SEQuoia complete RNAseq kit (Bio-Rad cat# 17005726) following the manufacturer’s procedures without any modifications ...
-
bioRxiv - Plant Biology 2024Quote: ... higginsianum genomic DNA quantitation was performed by quantitative PCR using Biozym Blue S’Green qPCR Kit (Biozym Scientific, Germany) in a CFX RT-PCR detection system (Bio-Rad, USA) and specific primers for ChTrpC gene (Supplementary Table S1) ...
-
bioRxiv - Cell Biology 2024Quote: ... Proteins were then blotted onto polyvinylidene difluoride (PVDF) membranes using Trans-Blot Turbo™ Transfer Starter System and Midi PVDF kit (Both are from Biorad). After transfer ...
-
bioRxiv - Neuroscience 2024Quote: ... the gel was transferred to a LF PVDF membrane using the Trans-Blot Turbo RTA Midi 0.45 µm LF PVDF Transfer Kit (BIO-RAD, 1704275). After blocking in 5% skim milk powder (Sigma Aldrich ...
-
bioRxiv - Cancer Biology 2024Quote: ... Lysates were heat at 95°C for 10 min and then separated by SDS-PAGE electrophoresis on 10% acrylamide gels (FastCast™ Acrylamide kit, BioRad) at 110V for 60 min ...
-
bioRxiv - Cell Biology 2024Quote: Total RNA was isolated from Rat lung tissue and subjected to cDNA preparation by the reverse transcription reaction using an iScript cDNA Synthesis Kit (#17088890, Bio-Rad). qPCR analysis was performed in triplicate using iQ SYBR Green Supermix (#1708882 ...
-
bioRxiv - Microbiology 2024Quote: RNA extracts were reverse transcribed into cDNA using an iScript™ Select cDNA Synthesis Kit (Bio-Rad, Marnes-la-Coquette, France) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2024Quote: ... Quantitative RT-PCRs (qRT-PCRs) were carried out in triplicate with three technical replicates with the SsoAdvanced Universal SYBR Green kit (Bio-Rad) on a CFX384 Touch Real-Time PCR Detection System (BioRad) ...
-
bioRxiv - Microbiology 2024Quote: We carried out reverse transcription with RNA prepared for transcriptome analysis using an iScript cDNA synthesis kit (Bio-Rad, Hercules, CA). The cDNA product was diluted 10-fold with water and we used 1Lμl per technical replicate for quantitative PCR analysis ...
-
bioRxiv - Immunology 2024Quote: ... The following cytokines and chemokines were measured using a Bioplex Express Kit according to the manufacturer’s directions (Biorad, Hercules, CA, USA): GM-CSF ...
-
bioRxiv - Microbiology 2024Quote: ... A total of 500 ng of resulting total RNA was used to synthesize cDNA via random priming utilizing the iScript™ cDNA Synthesis Kit (Biorad). Real-time qPCR was performed on QuantStudio 6 Pro (Applied Biosystems ...
-
bioRxiv - Immunology 2024Quote: ... from colonic samples of DR3.IL17A-/- (n = 5) and DR3 mice (n = 5) were reverse transcribed to cDNA using the High-Capacity iScript cDNA synthesis kit (Bio-Rad). qPCR reactions were then carried out in triplicate using 50 ng of cDNA and the Applied Biosystems Power SYBR Green PCR Master Mix (Applied Biosystems) ...
-
bioRxiv - Genetics 2024Quote: 500 ng of RNA was converted to cDNA using the iScript cDNA synthesis kit (#1708891, Bio-Rad, Hercules, CA, United States) containing random hexamers according to the manufacturer’s instructions ...
-
bioRxiv - Genetics 2024Quote: ... RNA concentrations were measured with the Little Lunatic (Unchained Labs) and cDNA was synthesized using the iScript cDNA synthesis kit (Bio-rad). Primers for skeletal marker genes were designed using NCBI Primer-BLAST (Supplementary Table 3 ...
-
bioRxiv - Developmental Biology 2024Quote: 100 ng of enriched small RNA fraction was used for generated cDNA following iScript Select cDNA Synthesis Kit (#708896, Bio-Rad) following manufacture’s protocol and the specific primer 5′-GCAGGTCCAGTTTTTTTTTTTTTTTCTACCCC-3′ (Supp ...
-
bioRxiv - Developmental Biology 2024Quote: ... cDNA for measuring primary-miR430 was generated using 200 ng of total purified RNA following iScript cDNA synthesis kit (#1708890, Bio-Rad) manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2024Quote: ... Biotin signal was detected and quantified by chemiluminescence according to standard LightShift Chemiluminescent RNA EMSA Kit protocol using a ChemiDoc™ Touch Imaging System (BioRad).
-
bioRxiv - Plant Biology 2024Quote: ... One microgram of total RNA was used for a reverse transcriptase reaction using iScript cDNA Synthesis Kit (Catalog #170-8891; Bio-Rad). RT-qPCR analysis was conducted using iQ SYBRGreen Supermix (Bio-Rad ...
-
bioRxiv - Cell Biology 2024Quote: ... For western blotting the proteins were transferred to polyvinylidene difluoride (PVDF) membranes using the Trans-Blot Turbo RTA Mini PVDF Transfer Kit (Bio-Rad) and the Trans-Blot Turbo Transfer System (Bio-Rad) ...
-
bioRxiv - Synthetic Biology 2021Quote: ... radiodurans genomic DNA was isolated in agar plugs using the Bio-Rad CHEF Genomic DNA Plug Kit (Bio-Rad, CAT#170-3592) with an adapted protocol44 ...
-
bioRxiv - Cancer Biology 2021Quote: ... The signal was detected by a chemiluminescent western blot detection kit (Thermofischer) and the blots were developed and visualized under a ChemiDoc system (Amersham, Bio-Rad, USA). The primary antibodies used were Twist2 (GeneTex ...
-
bioRxiv - Developmental Biology 2021Quote: ... 500 ng RNA was used for cDNA synthesis for each sample using the iScript cDNA Synthesis kit (Bio-Rad, Cat. No. 1708890). A CFX96 Touch Real-Time PCR Detection System (Bio-Rad ...
-
bioRxiv - Genomics 2020Quote: ddPCR was performed on a Bio-Rad QX200 Droplet Digital PCR System using ddPCR Supermix for Probes (No dUTP) kit (Bio-Rad, 1863024) according to manufacturer’s protocols ...
-
bioRxiv - Developmental Biology 2021Quote: ... Proteins were detected using the EZ-ECL Chemiluminescence Detection Kit for HRP (Biological Industries) and ChemiDoc XRS+ Gel Documentation System (Bio-Rad Laboratories). Images were taken using the ImageLab™ software (Bio-Rad).
-
bioRxiv - Microbiology 2021Quote: ... the fluorescence derived from the incorporation of EvaGreen into the double-stranded PCR products was measured at the end of each cycle using the SsoFast EvaGreen Supermix 2X Kit (Bio-Rad, France). The results were analyzed using Bio-Rad CFX Maestro software ...
-
bioRxiv - Neuroscience 2022Quote: ... A total of 1 μg of RNA was subjected to retrotranscription using iScript™cDNA Synthesis Kit (Bio-Rad, Cat. No. 1708890), according to manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: ... 400 ng of complementary DNA (cDNA) was synthesized using the reverse transcriptase iScript complementary DNA synthesis kit (Bio-Rad, Hercules, CA; # 1708891), then diluted to a concentration of 2 ng/μL ...
-
Targeted rescue of synaptic plasticity improves cognitive decline after severe systemic inflammationbioRxiv - Neuroscience 2021Quote: 250 ng of total RNA of each sample was reversely transcribed and amplified using the TargetAmp-Nano Labeling Kit for Illumina Expression BeadChips (Biozym Scientific GmbH, Hessisch Oldendorf, Germany) on a Biorad MJ Thermal Cycler (Biorad, Munich, Germany) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: ... CLN5 transcripts were measured from the control and CLN5i neurons RNA by quantitative polymerase chain reaction[13] using an iTaq Universal Probes one-step kit (Bio-Rad, 1725141) on a Roche 96-well thermocycler ...
-
bioRxiv - Bioengineering 2022Quote: ... RNA was reverse transcribed using the High Capacity cDNA Reverse Transcription Kit (Thermo Fischer Scientific) and a T100™ thermal cycler (Bio-Rad). The expression of selected genes was determined by RT-PCR using the iQ Universal SYBR Green Supermix (Bio-Rad ...
-
bioRxiv - Microbiology 2022Quote: ... the fluorescence derived from the incorporation of EvaGreen into the double-stranded PCR products was measured at the end of each cycle using the SsoFast EvaGreen Supermix 2X Kit (Bio-Rad, France). The results were analyzed using the Bio-Rad CFX Manager software ...
-
bioRxiv - Molecular Biology 2020Quote: ... Isolated RNA (50 ng) was subjected to qRT-PCR analysis using iTaq™ Universal SYBR® Green One-Step Kit (Bio-Rad) in the 7500 Real-Time PCR System (Applied Biosystems) ...
-
bioRxiv - Immunology 2019Quote: ... First strand synthesis and real time PCR were performed using iScript kit and iTaq Universal SYBR Green Supermix (Bio-Rad, Hercules, CA) on Light Cycler 480 (Roche ...
-
bioRxiv - Genomics 2019Quote: Mammalian cells were embedded in gel plugs and High Molecular Weight DNA was purified as described in a commercial large DNA purification kit (BioRad #170-3592). Plugs were incubated with lysis buffer and proteinase K for four hours at 50°C ...
-
bioRxiv - Immunology 2019Quote: ... One microgram of RNA was used to synthesize first strand cDNA using the iScript cDNA synthesis kit from Bio-Rad (Hercules, CA). The primers used for qPCR were synthesized by IDT (Coralville ...
-
bioRxiv - Cell Biology 2020Quote: ... HRP-conjugated secondary anti-mouse antibody (Amersham; NA931S) and anti-rabbit antibody (Amersham; NA934VS) at 1:10,000 and Clarity western ECL substrate detection kit (Bio-Rad; 170-5060) were used to detect the signals ...
-
bioRxiv - Immunology 2019Quote: ... and 1 µg of RNA was used as input for cDNA synthesis using the iScript cDNA synthesis kit (Bio-rad, cat. #: 1708890). cDNA was diluted to 20 ng/µl and 2.5 µl/reaction was used as input for the qPCR reaction ...
-
bioRxiv - Cancer Biology 2019Quote: One microgram of total RNA was used for cDNA synthesis using the iScript cDNA Synthesis Kit (Bio-Rad Laboratories, Inc., Hercules, CA). The PCR reactions were performed with 0.2 µl of cDNA template in 25 µl of reaction mixture containing 12.5µl of iQ SYBR Green Supermix (Bio-Rad Laboratories ...
-
bioRxiv - Biochemistry 2019Quote: ... Gene silencing efficiencies were performed as described previously18 with the following modifications: cDNA was synthesized from 1 μg total RNA using the iScript cDNA Synthesis Kit (Bio-Rad, #1708891) following the manufacturer’s instructions and qRT-PCR was performed with a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems ...
-
bioRxiv - Immunology 2019Quote: ... Quantification of gene expression was performed by using the KAPA SYBR-FAST One-Step qRT-PCR kit and the CFX-Connect Real-time PCR detection system (Bio-Rad Laboratories) following the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2020Quote: ... and total RNAs were obtained according to the manufacturer’s protocol and then quantified and synthesized into cDNA using a iScript cDNA Synthesis Kit (Bio-Rad, Hercules, USA) for SALL4 expression or using a High-capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2019Quote: ... we synthetized 100ng of cDNA from immunoprecipitated and isolated polyribosome-associated RNA (see above for detailed procedure) as well as non-precipitated RNA (input) using the iScript cDNA synthesis kit (Bio-Rad, USA). cDNA was then preamplified using TaqMan assay following kit instructions46 ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Samples were reverse transcribed from 500 ng of total RNA in a 20 µL volume using iScript cDNA synthesis kit (Bio-Rad Laboratories). Real-time qPCR was carried out on a C1000™ Thermal Cycler (Bio-Rad Laboratories) ...
-
bioRxiv - Genomics 2019Quote: ... 1 μg of each RNA sample was converted into single-strand cDNA with random hexamers using an iScript™ Select cDNA Synthesis Kit (Bio-Rad). To validate fusion transcripts ...
-
bioRxiv - Neuroscience 2020Quote: ... Up to 1 μg of RNA was used to synthesise cDNA using the iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad, 1725035) and PowerUp SYBR Green (Thermofisher Scientific ...
-
bioRxiv - Biochemistry 2020Quote: ... PCR was performed using the AceQ qPCR SYBR Green Master Mix kit (Vazyme, Nanjing, China) and the MyiQ2 Real-time PCR system (Bio-Rad, USA) with the following cycling conditions ...