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Citations for Bio-Rad :
601 - 650 of 6542 citations for ssc mir 376c RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... cDNA was diluted five-fold and quantitative PCR (RT-qPCR) was performed using iQ SYBR Green Supermix (Bio-Rad) per manufacturer’s protocol on a 7900HT Sequence Detection System (Applied Biosystems ...
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bioRxiv - Cancer Biology 2023Quote: ... Quantitative Real rime PCR (RT-qPCR) analysis was performed on genes of interest (Supplementary Table 1) using SYBRGreen (BioRad). Sso Fast EvaGreen Supermix was performed to analyze gene expression on a BioRad CFX96 Real-Time System (BioRad ...
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bioRxiv - Biochemistry 2023Quote: Thermal shift assays were carried out in 96 well plates using the CFX96 RT-PCR detection system (Bio-Rad). Thermal stability experiments were performed in the reaction mixture containing LIG1 (5 µM ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting cDNA was used as input for RT-qPCR using CFX-384 Touch Real-Time PCR System (Biorad), Power SYBR Green PCR MasterMix (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: We quantified selenon transcripts in zebrafish using PrimePCR multiplex real time quantitative PCR (RT-qPCR) system by Bio-Rad. We extracted and purified total RNA from cells and zebrafish using Trizol LS reagent (Ambion ...
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bioRxiv - Molecular Biology 2022Quote: Reverse transcription (RT) was performed with the iScript RT kit (BioRad) using 10 µL (0.50-0.95 µg ...
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bioRxiv - Molecular Biology 2021Quote: PCR reactions were set up using the following volumes: 10 µL 2x ddPCR Supermix for probes (no dUTP) (BioRad Laboratories© 186-3024), 5 µL of 20plex oligo mix ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... miR-146a-loaded MLNPs were lysed with 0.5% sodium dodecyl sulfate (SDS) (BioRad, Hercules, CA), and loaded onto 2% agarose (Fisher BioReagents ...
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bioRxiv - Genetics 2020Quote: ... with 500 nmol primers were used for qPCR analyses on a CFX96 Touch Real-Time PCR Detection System (#1855196, Biorad). Primers used were home-made designed and tested (Supplementary Table S2) ...
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bioRxiv - Plant Biology 2021Quote: Quantitative real-time PCR analysis was performed using gene-specific primers (Supplemental Table S2) and iQ SYBR Green Supermix (Biorad) in iCycler5 IQ (Biorad ...
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bioRxiv - Neuroscience 2022Quote: ... aliquots of first-stranded cDNA were amplified with gene-specific primers and Power SYBR Green PCR Master Mix (Bio-Rad) using a Step-1 Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Plant Biology 2022Quote: ... Quantitative PCR was performed using TaqMan primers synthesized by Integrated DNA Technologies (Table S2) on a CFX96 C1000 Touch Real-Time PCR Detection System (BioRad). ZmCyanase expression was used to normalize among biological samples ...
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bioRxiv - Plant Biology 2021Quote: ... 10 µl PCR reactions were performed in technical duplicate with SensiFAST SYBR No-ROX (Meridian Bioscience) with 4 ng cDNA and 300 nM primers (Table S1) on a CFX96 Real-time PCR System (BioRad). Mean PCR reaction efficiencies were calculated for each primer pair with LinRegPCR (Ruijter et al. ...
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bioRxiv - Immunology 2020Quote: ... Gene expression was measured by quantitative real-time PCR using gene-specific primers and SYBR Green (Biorad, Hercules, CA, USA). For quantification of the abundances of miR-30e ...
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bioRxiv - Immunology 2020Quote: ... Gene expression was measured by quantitative real-time PCR using gene-specific primers and SYBR Green (Biorad, Hercules, CA, USA). The 18S gene was used as a reference control ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was extracted from cells transfected with pCMVΔR8.91 using TRIzol LS reagent and PCR amplified with primers DR22 and DR23 using IQ SYBR Green Supermix (BioRad, 1708882). Total RNA from 0.33×106 cells was used for electrophoretic analysis of rRNA integrity with an Agilent Technologies 4200 TapeStation ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and 0.5 μM of gene-specific primers (Sup Table 1) in a CFX96 qPCR Real-Time PCR module with C1000 Touch Thermal Cycler (BioRad). After 10 minutes at 95°C ...
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Insight into the regulatory mechanism of the MFS transporter, SCO4121 by the MarR regulator, SCO4122bioRxiv - Molecular Biology 2024Quote: ... Expression studies were further carried out using 100 ng of cDNA template and 0.5 µM (each) of specific primers in a Bio Rad CFX96 real time PCR with SyBr Green iTaq Mix (Biorad). Data generated was further analysed using 2-ΔΔCT method ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative PCR with gene-specific primers was performed with the SsoAdvanced SYBR Green Supermix (Bio- Rad) on a CFX96 Real-Time PCR detection system (BioRad). Signals were normalized to the reference genes ACTIN2 using the ΔCt method and the relative expression of a target gene was calculated from the ratio of test samples to Col-0 ...
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bioRxiv - Immunology 2023Quote: ... and IRF7) (Supporting Table-S4) using Primer-BLAST (NCBI) and real-time PCR was performed using a CFX96 Touch System (Biorad). Real-time PCR protocol started with an initial denaturation and enzyme activation at 95°Cfor 2 minutes followed by 40 cycles of denaturation at 95°Cfor 15 seconds ...
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bioRxiv - Plant Biology 2023Quote: ... cDNA was diluted and target gene expression assessed by qRT-PCR using gene-specific primers and SsoAdvanced Universal SYBR Green Supermix (BioRad). Detailed information regarding all germplasm generated or used in this study ...
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bioRxiv - Plant Biology 2023Quote: ... a standard curve was generated to estimate the PCR efficiency of each primer pair using CFX Maestro software v.2.0 (Bio-Rad). The slope and coefficient of determination (R2 ...
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bioRxiv - Microbiology 2020Quote: ... were pre-soaked with 10X SSC for 5 min and assembled in dot-blot apparatus (Bio-Rad) with vacuum-on ...
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bioRxiv - Molecular Biology 2022Quote: ... was wetted in 20X SSC buffer and secured in a Bio-Dot Microfiltration Apparatus (Bio-Rad 1706545) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... iScript RT Supermix for RT-qPCR (Bio-Rad Laboratories, Hercules, CA, USA) was employed for the synthesis of first-strand cDNA ...
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bioRxiv - Cancer Biology 2020Quote: ... and then used for RT-mediated cDNA synthesis (cDNA RT kit, BioRad), following which PCR was performed with primers specific for Il-12b and β-actin ...
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bioRxiv - Neuroscience 2024Quote: ... RT-qPCR was performed on a CFX96 RT-qPCR system (Bio-Rad) using the following conditions ...
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bioRxiv - Neuroscience 2020Quote: ... 500 ng of RNA was used for Realtime RT-PCR analysis using iTaq universal SYBR Green One-Step Kit (BioRad) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR plates were sealed using optically clear films and transferred to a C1000 thermal cycler with CFX-96 RT-PCR head (BioRad). The fluorescence intensity was measured over a temperature gradient from 25– 95 °C at a constant rate of 0.05 °C/s ...
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bioRxiv - Bioengineering 2020Quote: ... Quantitative real time RT-PCR (QPCR) were performed on the Opticon Continuous Fluorescence System (Bio-Rad Laboratories, Inc., Hercules, CA) using the QuantiTect SYBR Green RT-PCR kit (Qiagen) ...
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Transmembrane Signaling Regulates the Remodeling of the Actin Cytoskeleton: Roles of PKC and AdducinbioRxiv - Cell Biology 2021Quote: ... RT/PCR products were analyzed by 8% polyacrylamide gel and relative band intensity was quantified using Image Lab (Bio-Rad). Most of the primer sequences used were selected from the FlyPrimerBank (Hu et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... RT-PCR for the for gene expression analysis was carried out using the CFX-96 thermocycler (Bio-Rad, Mississauga, ON) using the following primers ...
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bioRxiv - Biophysics 2021Quote: ... RT-qPCR was performed on a CFX96 Touch™ Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules, CA, USA), by 40 cycles of denaturation at 95 °C for 30 s ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative RT-PCR was performed on a BioRad CFX connect Real-time system using universal SYBR® Green supermix (BioRad). Relative gene expression was calculated using the 2-ΔΔCT method ...
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bioRxiv - Cancer Biology 2022Quote: ... and RT-qPCR was performed using iQ SYBR Green Supermix and the iCycler Real-time PCR detection system (Bio-Rad). Primer nucleotide sequences (Table S1 ...
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bioRxiv - Plant Biology 2021Quote: ... was used for RT-qPCR using three technical replicates per reaction in a CFX96 Touch Real-Time PCR Detection System (Biorad). Primers are described in Table S4 ...
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bioRxiv - Neuroscience 2021Quote: ... The resulting cDNAs were used as a template for RT-qPCR using a CFX96 Real-Time PCR Detection System (Biorad) with a SYBR Green master mix (Qiagen) ...
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bioRxiv - Biophysics 2021Quote: ... according to kit protocol and 10 ng of cDNA were analyzed for TMPRSS2 expression by real-time RT-PCR on a CFX Connect Real Time System using SsoAdvancedSybrGreen Supermix (BioRad). The amplification protocol was 2 min at 95°C ...
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bioRxiv - Bioengineering 2021Quote: ... Quantitative real time RT-PCR (QPCR) was performed on the Opticon Continuous Fluorescence System (Bio-Rad Laboratories, Inc., Hercules, CA) using the QuantiTect SYBR Green RT-PCR kit (Qiagen) ...
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bioRxiv - Immunology 2022Quote: ... Expression analysis was conducted in duplicate via RT– PCR on a Bio-Rad CFX384 using iTaqTM SYBR (Bio-Rad, 1725125). Expression levels were quantified and normalized to Gapdh expression ...
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bioRxiv - Evolutionary Biology 2020Quote: ... or ∼50 ng (8-14 months post-hatch) of total RNA as template in one-step reverse transcription droplet digital PCR (RT-ddPCR) [66] (BioRad) with TaqMan probes specific to vgll3 alleles to quantify vgll3 absolute expression (Supplementary table 1) ...
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bioRxiv - Bioengineering 2020Quote: A reverse transcription polymerase chain reaction (RT-PCR) was carried out on the RNA using the mix iScript Reverse Transcription Supermix (Biorad) and the following thermocycler program ...
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bioRxiv - Neuroscience 2022Quote: ... was used for the Reverse Transcription-Polymerase Chain Reaction (RT-PCR) by CFX96 Touch qPCR Detection System (Bio-Rad, 1855196). Data was analysed by CFX Manager software (Bio-Rad).
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bioRxiv - Developmental Biology 2020Quote: ... GEM-RT-PCR was performed in a C1000 Touch Thermal cycler with 96-Deep Well Reaction Module (Bio-Rad; CT022510) to produce barcode cDNA using the following program ...
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bioRxiv - Bioengineering 2022Quote: RNA was extracted as described above, followed by RT-PCR using the Allplex SARS-CoV-2 assay (SeeGene, South-Korea) on a CFX96 thermocycler (Biorad), simultaneously detecting four different target genes ...
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bioRxiv - Bioengineering 2022Quote: ... droplet-digital PCR was performed for 16 extracted RNA samples using the 1-Step RT-ddPCR Advanced Kit for Probes (BioRad) and the CFX96 thermocycler (BioRad ...
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bioRxiv - Plant Biology 2022Quote: ... PCR plates were sealed using optically clear films and transferred to a C1000 thermal cycler with CFX-96 RT-PCR head (BioRad). The fluorescence intensity was measured over a temperature gradient from 25 to 95 °C at a constant rate of 0.05 °C/s ...
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bioRxiv - Plant Biology 2022Quote: ... RT-PCR was performed using i-Taq™ DNA Polymerase (Intron) and a T100™ Thermal Cycler system (Bio-Rad). Real-time quantitative RT-PCR (qRT-PCR ...
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bioRxiv - Microbiology 2022Quote: ... on cDNA samples using the LightCycler 480 SYBR Green I Master kit in the CFX384 RT-PCR Detection System (Biorad). The identities of the resulting amplicons were checked by melting-curve analysis using the CFX384 software ...
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bioRxiv - Cancer Biology 2024Quote: ... A total of 1µg of RNA in 20µl reaction volume was reverse transcribed using the iScript Reverse Transcription Supermix for RT-PCR (Bio-Rad). RT-qPCR was performed for TNFA ...