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Citations for Bio-Rad :
551 - 600 of 6542 citations for ssc mir 376c RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... qRT-PCR was performed in 10µl reactions with 0.2 µM forward and reverse primers on a CFX Connect (Biorad) thermocycler ...
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bioRxiv - Immunology 2024Quote: ... Gene expression measured by Quantitative real-time PCR using gene specific primers with CYBR green chemistry (Bio-Rad), 18S RNA was used as an internal control for normalization ...
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bioRxiv - Microbiology 2021Quote: ... Reactions were performed in triplicate in a total volume of 20 μL that included 2 μL of the template or standard plasmid and 250 nM of each primer and TaqMan (5’ hydrolysis) probe (the RT-qPCR reactions also included 0.5 μL of Bio-Rad iScript advanced reverse transcriptase). Thermocycling conditions for qPCR consisted of an initial 50 °C step for 2 min followed by 95 °C for 10 min ...
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bioRxiv - Plant Biology 2020Quote: Relative expression of CgCOM1 transgene in chilli- and tomato-CgCOM1i transgenic lines was analyzed by RT-qPCR using CgCOM1 gene specific primers (Supplementary Table S1) and SSoFast™ EvaGreen® supermix (Bio-Rad, USA) on CFX96 Touch™ Real-Time PCR detection system (Bio-Rad ...
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bioRxiv - Developmental Biology 2020Quote: ... and then amplified by real-time quantitative polymerase-chain reaction (RT-qPCR, SybrGreen PCR MasterMix Kit, Bio-Rad) using primers listed in Table 1 ...
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bioRxiv - Molecular Biology 2021Quote: ... Quantitative RT-PCR was performed on a BioRad CFX Connect system using iTaq Universal SYBR Green Supermix (BioRad). Designed primers were acquired from Integrated DNA Technologies and provided in Table S2 ...
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bioRxiv - Neuroscience 2019Quote: ... The RT-qPCR was performed on a CFX96 C1000 Touch™ Real-Time PCR detection system (Bio-Rad) with thermal cycling conditions as follows ...
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bioRxiv - Plant Biology 2019Quote: ... Quantitative RT-PCR was carried out in a CFX Connect™ Real Time System (BioRad, Hercules, CA, USA) using iQ™ SYBR® Green Supermix (BioRad ...
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bioRxiv - Cell Biology 2021Quote: ... RT-qPCR was carried out to manufacturer specifications in a CFX96 Touch Real Time PCR Machine (Bio-Rad). CLB2 ...
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bioRxiv - Microbiology 2020Quote: ... quantitative real-time RT-PCR assays were performed using the iTaq SYBR Green One-Step Kit (Bio-Rad) on the LightCycler 480 system (Roche ...
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bioRxiv - Plant Biology 2021Quote: ... Quantitative RT-PCR reactions were carried out in 5μL using the SsoAdvanced Universal SYBR Green Supermix from BioRad and the following program on QuantStudio™ 5 (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2022Quote: ... RT-qPCR (Real-Time quantitative PCR) was performed using iQ SYBR Green Supermix solution (Bio-Rad, #170-8880). The CFX96 Real-Time PCR Detection System (Bio-Rad Laboratories ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Gene expression was investigated by quantitative reverse transcriptase PCR (RT-qPCR) using SsoFastTM EvaGreen® Supermix (Bio-Rad). Protein expression was assessed via immunofluorescence staining and microscopy ...
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bioRxiv - Physiology 2022Quote: Quantitative RT-PCR was carried out in a CFX96 Real-Time System (Bio-Rad Laboratories, Hercules, CA, USA). RNA was extracted with the RNeasy Micro kit (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... quantitative real-time RT-PCR assays were performed using the iTaq SYBR Green One-Step Kit (Bio-Rad) on the LightCycler 480 system (Roche ...
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bioRxiv - Cancer Biology 2020Quote: ... The RT-qPCR was performed on a CFX384 Touch Real-Time PCR Detection System (Bio-Rad, cat#1855485). Supplemental Table 4 outlines the reaction conditions ...
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bioRxiv - Microbiology 2021Quote: ... a quantitative RT-PCR assay was conducted using an iTaq Universal SYBR Green one-step kit (Bio-Rad) on a QuantStudio 7 Flex Real-Time PCR Systems (Thermo fisher ...
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bioRxiv - Immunology 2021Quote: ... Real Time-quantitative PCR (RT-qPCR) was performed using the SsoFast EvaGreen Supermix (Bio-Rad Laboratories, CA, USA) and the primer pairs described in Table 1 ...
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bioRxiv - Molecular Biology 2024Quote: Two-step RT-qPCR was performed on a CFX96 Real-Time PCR Detection System (Bio-Rad, Hercules, CA) utilizing SYBR Green I chemistry ...
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bioRxiv - Developmental Biology 2024Quote: ... RT-PCR was performed on 1:10 cDNA dilutions using Sso Fast EvaGreen supermix (Bio-Rad, 172-5280) on a thermocycler (BioRad ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The resulting complete LhGAP3 sequence was then verified by RT-PCR using the iScript cDNA Synthesis Kit (BioRad) and the GoTaq DNA Polymerase (Promega) ...
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bioRxiv - Immunology 2023Quote: ... and cDNA was synthesized using iScript reverse transcription Supermix for RT-PCR (Bio-Rad Laboratories, Inc., CA, USA). The qPCR reactions were performed using iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative RT-PCR was performed on a BioRad CFX Connect system using iTaq Universal SYBR Green Supermix (BioRad). Designed primers were acquired from Integrated DNA Technologies and are provided in Table 2 ...
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bioRxiv - Cancer Biology 2022Quote: ... and real-time RT-PCR analyses were performed in triplicates using the iQ5 Optical System from Bio-Rad with SYBR Green mix (Life Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... Quantitative mRNA expression was determined by real-time RT-PCR using ITaq Universal SYBR Green Supermix (Bio-Rad). For a list of primers used ...
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bioRxiv - Cancer Biology 2024Quote: Reverse transcription quantitative real-time PCR (RT-qPCR) reactions were performed in 96-well plates (BioRad CFX platform) by using SyBr green in a total reaction volume of 10μl per well ...
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bioRxiv - Plant Biology 2024Quote: ... RT-qPCR was performed in a 384 well thermocycler (CFX384 TouchTM Real-Time PCR Detection System, Bio-Rad) using the GoTaq qPCR Mastermix (Promega ...
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bioRxiv - Genomics 2024Quote: Using the iScript RT Supermix for RT-qPCR kit (Biorad), 10 μL of RNA (concentration of 80 ng/μL ...
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bioRxiv - Developmental Biology 2021Quote: ... qPCR analysis was performed using the primers in Table S1 on CFX384 Touch Real-Time PCR Detection System (BioRad). Data were analysed using the CFX Maestro Software (BioRad) ...
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bioRxiv - Genomics 2020Quote: ... Primers were tested before use by quantitative real-time PCR on a CFX Connect (Bio-Rad Laboratories; Hercules, CA).
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bioRxiv - Microbiology 2020Quote: ... PCR reactions for each sample were performed in triplicate with specific primers using iQ SYBR Green Supermix (1708887, Biorad). Relative gene expression was quantified using the 2−ΔΔCT method (90) ...
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bioRxiv - Neuroscience 2021Quote: ... and specific primers for each gene (Supplementary Table) with a CFX384 Touch Real-Time PCR Detection System (Bio-Rad). Target genes were normalized to the geometric mean of control genes ...
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bioRxiv - Zoology 2019Quote: ... and specific primers (Table 1) on a CFX96 Touch™ Real-Time PCR Detection System (Biorad, Hercules, CA, USA). Actin was used as housekeeping gene normalization control (Table 1) ...
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bioRxiv - Biochemistry 2021Quote: ... Real-time PCR was then performed with each unique primer pair according to manufacturer’s protocols (iTaq Universal SYBR Green Supermix, BioRad) to quantify undigested (that is ...
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bioRxiv - Genetics 2020Quote: Quantitative PCR (qPCR) was performed using 400nM primer and 50% v/v iQ SYBR green master mix (Bio-rad) together with DNA in 25µL final volume ...
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bioRxiv - Cell Biology 2022Quote: ... TaKaRa, Cat ID RR820B) and primers (Bioserve, India) for validation of mRNA levels using quantitative real-time PCR (BioRad CFX96 Touch Real-Time PCR Detection System).
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bioRxiv - Plant Biology 2023Quote: ... with 7.5 ng cDNA and 200 nM primers (Table S1) on a CFX Opus 384 Real-time PCR System (BioRad). Mean PCR reaction efficiencies were calculated for each primer pair with LinRegPCR (Ruijter et al. ...
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bioRxiv - Genomics 2023Quote: ... For quantitative real-time PCR 20 ng cDNA and 1 pmol/μl primers were mixed with 2x SYBR (BioRad) in a total volume of 13 μl for each well ...
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bioRxiv - Plant Biology 2024Quote: ... with gene-specific primers (20 μM) in a CFX96 Touch Deep Well Real-Time PCR detection system (Bio-Rad). Two μl of initial cDNA were used as template in 10 μl qPCR reaction to evaluate the expression of reference genes ...
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bioRxiv - Molecular Biology 2024Quote: ... The primers used in our experiments included primers purchased from Bio-Rad (Srebf1 ...
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bioRxiv - Cell Biology 2020Quote: ... 1μg of total RNA of each sample was used for cDNA synthesis using RT-PCR System (Bio-Rad, USA) under following conditions ...
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bioRxiv - Developmental Biology 2020Quote: ... Evaluation of results was performed in the CFX96 Touch RT-PCR Detection System program (Bio-Rad, Hercules, CA, USA) and using GraphPad Prism 8 software (Prism ...
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bioRxiv - Neuroscience 2021Quote: ... The threshold amplification cycle number (Ct) was determined for each reaction by Quantitative RT-PCR (Bio-Rad CFX 96) in the linear phase of the amplification plot ...
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bioRxiv - Molecular Biology 2020Quote: ... The quantitative real-time RT-PCR analysis was performed by iTaqTM Universal SYBR Green Supremix (#172-5124, BIO-RAD). The reaction mixtures containing SYBR Green were composed following the manufacturer’s protocol and then CT values were obtained using a qPCR platform (QuantStudio 6 Flex Real-Time PCR System ...
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bioRxiv - Molecular Biology 2020Quote: ... Five nanograms cDNA was used for RT-qPCR analysis on CFX96 Touch Real-Time PCR Detection System (Bio-Rad) using the following primer pairs:
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bioRxiv - Bioengineering 2022Quote: ... The expression of selected genes was determined by RT-PCR using the iQ Universal SYBR Green Supermix (Bio-Rad) and a StepOnePlus real-time PCR equipment (Applied Biosystems) ...
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bioRxiv - Pathology 2022Quote: ... All RT-qPCR plates were run on a CFX96 Touch real-time PCR detection system (Bio-Rad, CA, USA). Primers utilized for RT-qPCR validations (see Supplementary Table 2 ...
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bioRxiv - Developmental Biology 2022Quote: ... qPCR was performed similar to fetal liver except using a CFX96 RT-PCR Detection System (Bio-Rad, Hercules, CA). Placental results were normalized to ACTB using the comparative Ct method ...
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bioRxiv - Genetics 2020Quote: ... digital droplet PCR (ddPCR) was performed using the One-step RT-ddPCR advanced kit for probes (Bio-Rad, #1864022). The final 20 μL reaction mix contained ...
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bioRxiv - Biochemistry 2021Quote: ... using a 96-well Bio-Rad CFX96 RT–PCR System with a C1000 Thermal Cycler (Bio-Rad, Hercules, CA) according to the manufacturers’ instructions ...