Labshake search
Citations for New England Biolabs :
551 - 600 of 615 citations for Universal TT epitope P2 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... One-step quantitative RT-PCR was used to determine gene expression levels using the Luna Universal One-Step RT-qPCR kit (New England Biolabs Inc.) according to the manufacturer’s recommendations using 4 ng of total RNA per 20µl reaction ...
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bioRxiv - Microbiology 2020Quote: ... RT-qPCR reactions were carried out using LightCycler 96 instrument and following reagents: Luna Universal One-Step RT-qPCR Kit (New England Biolabs, NEB) for hCoV-229E and hCoV-OC43 ...
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bioRxiv - Genomics 2021Quote: ... TF motif libraries in pGL4.10-Sasaki-SS (a) and pCpG-free-EF1α-SS (b) vectors were amplified using standard Illumina Universal and index primers (NEB #E7335S) and sequenced using standard Illumina chemistry ...
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bioRxiv - Genetics 2020Quote: ... Each reaction was run in 10μL volumes and included 1x Luna Universal qPCR Master Mix (New England BioLabs, Ipswich, Mass. USA), optimal forward and reverse concentrations were experimentally determined (200nM for both forward and reverse primers for 17S ...
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bioRxiv - Cell Biology 2020Quote: ... 200 ng/μL was used for subsequent reverse transcription assay with Luna® Universal One Step RT-qPCR kit (E3005, New England Biolabs) according manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... with a 384 well plate using the NEB Luna Universal One-Step RT-qPCR kit (NEB #E3005L, New England Biolabs Inc) and a reaction volume of 10 μl with 2.5 μl of sample ...
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bioRxiv - Microbiology 2020Quote: ... with a 384 well plate using the NEB Luna Universal One-Step RT-qPCR kit (NEB #E3005L, New England Biolabs Inc) and a reaction volume of 10 μl with 2.5 μl of sample ...
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bioRxiv - Immunology 2021Quote: ... 10 µL diluted cDNA was used with the NEB Luna® Universal Probe qPCR Master Mix (New England BioLabs® Inc.) following the manufacturer’s protocol and performed in 384-well plates using a QuantStudio™ 5 Real-Time PCR System (Applied Biosystems™) ...
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bioRxiv - Genomics 2021Quote: ... in a 20 μl reaction mixture consisting of Luna® Universal qPCR Master Mix (New England Biolabs, Inc., Cat. No.: NC1276266), forward and reverse primer pairs (F515-GTGCCAGCMGCCGCGGTAA and R806-GGACTACHVGGGTWTCTAAT ...
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bioRxiv - Microbiology 2022Quote: ... 1-step RT-qPCR was performed on 2μL of RNA using the Luna® Universal One-Step RT-qPCR Kit (NEB Biosciences) and primers for β-tubulin (F ...
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bioRxiv - Cell Biology 2022Quote: ... were incubated with 25 μL of containing TF or 18S primers and fluorescent Luna® Universal qPCR Master Mix (New England Biolabs), and qRT-PCR was carried out in triplicate for each sample on the CFX Connect real-time System (Bio-Rad Laboratories).
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bioRxiv - Developmental Biology 2022Quote: ... cDNA was diluted 1:25 in water and used as template for RT-qPCR using the Luna Universal qPCR master mix (NEB M3003S). Primers used are listed in Supplementary Table 3 ...
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bioRxiv - Cell Biology 2022Quote: ... Real-time PCR was performed to quantify the expression level of mRNAs by Luna® Universal qPCR Master Mix (NEB, M3003E) and LightCycler ®480 Real-Time PCR System (Roche) ...
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bioRxiv - Microbiology 2022Quote: ... Purified RNAs were used for library preparation using the NEB Next Multiplex Small RNA Library Prep kit for Illumina (E7300 L) with Universal miRNA Cloning Linker from Biolabs (S1315S) as the 3’ adaptor and in-house designed indexed primers ...
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bioRxiv - Plant Biology 2022Quote: ... Double promoter constructs were constructed by reamplifying the promoter region of interest with unique overhangs and cloning into the Level −1 universal acceptor backbones using the BsaI-HFv2 restriction enzyme (#R3733L New England Biolabs, USA). These parts were then assembled into the same Promoter + 5’ UTR acceptor backbone using the BpiI restriction enzyme (#ER1012 Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... primers AngiCOI_forward (S0963) and AC1R (S0966) were used in a qPCR reaction with Luna® Universal qPCR Mastermix (New England Biolabs, Australia). The qPCR reactions were run on the CFX96 Touch Real-Time PCR Detection System (BioRad ...
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bioRxiv - Plant Biology 2022Quote: ... 1.5ul of the resulting cDNA was used as template in 20ul scale qPCR reactions using Luna Universal qPCR master mix (New England Biolabs M3003) with forward primers specific to the microRNA of interest and a universal reverse primer ...
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bioRxiv - Microbiology 2023Quote: ... RNA was diluted 1:50 for RT-qPCR which was conducted using the Luna Universal One-Step RT-qPCR kit (New England Biolabs E3005E) on a C1000 touch thermal Cycler / CFX384 Real-Time System (Biorad) ...
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bioRxiv - Microbiology 2023Quote: RNA was used as template to detect and quantify viral genomes by duplex reverse transcriptase (RT) quantitative polymerase chain reaction (RT-qPCR) using a Luna Universal Probe one-step RT-qPCR kit (New England Biolabs, E3006E). SARS-CoV-2-specific RNAs were detected by targeting the ORF1ab gene using the following set of primers and probes ...
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bioRxiv - Microbiology 2023Quote: ... Quantification is based on real-time SYBR green amplification molecules with specific target primers using Luna® Universal qPCR Master Mix (Biolabs). Relative mRNA expression is calculated using the CFX Manager software (Bio-Rad) ...
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bioRxiv - Genomics 2022Quote: ... mScarlet and HPRT (housekeeping gene for normalization) levels were then measured by qPCR using the Luna Universal One-Step RT-qPCR Kit (NEB #E3005) with 100ng total RNA per reaction ...
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bioRxiv - Genomics 2023Quote: ... 21 µl of DNA was mixed with 2 µl of universal i5 and i7 primers (Buenrostro et al. 2015) and 25 µl of NEBNext HiFi 2X PCR master mix (NEB #M0544). Samples were amplified in a thermocycler as follows ...
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bioRxiv - Microbiology 2023Quote: ... Region of Cori and ter were quantitatively amplified using the Sybr green-based Luna Universal One-step RT-qPCR kit (New England Biolabs Inc.), without the reverse transcriptase enzyme ...
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bioRxiv - Neuroscience 2023Quote: ... Titration of viral particles was performed by quantitative PCR (qPCR) using serial dilutions of purified AAV concentrates and Luna Universal qPCR mix (NEB#M3003). Oligonucleotide primers for AAV titration purpose were forward woodchuck hepatitis virus post-transcriptional regulatory element (WPRE ...
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bioRxiv - Genetics 2023Quote: ... cDNA was then used as a template for qPCR using the Luna Universal Probe Master Mix (New England Biolabs, Ipswich, Massachusetts) with the Universal Probe Library #21 probe (Roche ...
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bioRxiv - Biochemistry 2023Quote: ... cDNA synthesis and qRT-PCR were performed in a one-pot reaction using Luna® Universal One-Step RT-qPCR Kit (NEB). A QuantStudio Real-Time PCR System (ThermoFisher ...
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bioRxiv - Microbiology 2024Quote: ... RT-qPCR reactions were carried out in 10 μL reactions using the Luna® One-Step Universal RT-qPCR kit (NEB) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: RT-qPCR was performed (in triplicate) using the Bio-Rad cycler CFX 96 and the Luna universal qPCR master mix (NEB, M3003X). Gene expression levels were normalized to housekeeping genes EF1 ...
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bioRxiv - Microbiology 2020Quote: ... bacilliformis total RNA (for each 25 µl reaction) collected from various conditions using the Luna Universal One-Step RT-qPCR kit (New England BioLabs; Ipswich, MA) according to the manufacturer ...
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bioRxiv - Developmental Biology 2021Quote: ... Probes for Po-EgfrA were synthesized from a PCR template using universal T7 primers and T7 polymerase (New England Biolabs, MA, USA). RNA probes for Popi-Dfd were synthetized from the plasmid template of Popi-Dfd3’ using T7/T3 RNA polymerase (New England Biolabs ...
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bioRxiv - Microbiology 2022Quote: ... Reverse-transcription polymerase chain reaction (RT-PCR) was performed using the Luna Universal Probe One-Step RT-qPCR kit (New England BioLabs; https://www.neb.ca). The 5 ‘ untranslated region (UTR ...
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bioRxiv - Synthetic Biology 2022Quote: ... 20 µL qPCR reactions were carried out in duplicate according to the protocol provided with the Luna Universal Probe qPCR Master Mix (New England Biolabs, Ipswich, MA). Quantification was performed by comparison to a standard curve of E ...
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bioRxiv - Neuroscience 2021Quote: ... RT-qPCR was performed on a CFX Connect thermocycler with the Luna Universal or Probe one-step qPCR Master Mixes (New England Biolabs, Ispwich, MA). RNA (1-4 ng ...
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bioRxiv - Microbiology 2021Quote: ... and ∼ 90 ng was used for one-step RT-qPCR using Luna® Universal one-step RT-qPCR kit (New England BioLabs Inc.) [10 min ...
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bioRxiv - Microbiology 2020Quote: ... PCRs were performed in a final volume of 20 μl including NEB Luna Universal Probe One-Step Reaction Mix and Enzyme Mix (both New England Biolabs, Herts, UK) and 5 μl of RNA sample ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative PCR was performed by transferring 2 μl of the RT mix to the qPCR mix prepared with Luna Universal qPCR Master Mix (M3003, New England Biolabs, MA, USA), according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2019Quote: ... Measurement of differential gene expression was conducted using a qTower 2.2 (Analytik Jena, Jena, Germany) with the Luna® Universal One-Step RT-qPCR Kit (New England Biolabs, Ipswitch, USA). Primer pairs used for the analysis are listed in Table S3 D ...
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bioRxiv - Microbiology 2021Quote: ... mixtures for the detection of viral RNA were made by a Luna® Universal Probe One-Step RT-qPCR Kit (New England Biolabs, USA) in accordance with the manufacturer’s instructions and then placed in a thermocycler (BIO-RAD CFX96™ Real-Time System ...
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bioRxiv - Cell Biology 2021Quote: ... Reverse transcription and qPCR were performed in a one-step reaction using Luna® Universal One-Step RT-qPCR (NEB, Ipswich MA) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... qRT-PCR was subsequently performed with primers specific for edited genes and fly sequences using Luna Universal qPCR Mix (New England Biolabs, Ipswich, Massachusetts) and Biorad CFX ConnectTM Real-Time PCR Detection System (BioRad ...
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bioRxiv - Immunology 2022Quote: ... as described by Corman et al84 were used alongside the Luna Universal One-Step RT-qPCR kit (New England Biolabs, Ipswitch, MA) and CFX384 Touch Real-Time System (Bio-Rad ...
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bioRxiv - Developmental Biology 2022Quote: ... Four µl cDNA was used as input for 10 µl qPCR reactions with 2x Luna Universal qPCR Master Mix (NEB cat. #M3003X) and 0.25 µM forward and reverse primers (Extended Data Table 1 ...
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bioRxiv - Microbiology 2022Quote: ... DNA was subjected to qRT-PCR analysis using the Luna Universal qPCR Master Mix Protocol (New England Biolabs, Frankfurt am Main, Germany) and a CFX96 Real-Time System ...
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bioRxiv - Microbiology 2022Quote: ... immitis and Wolbachia qPCR assays were carried out in 10 μL final volumes of Luna® Universal Probe qPCR Master Mix (New England Biolabs, Australia) containing 1 μL of template DNA isolated from whole dog blood and primers and probe at a final concentration of 400 nM and 100 nM ...
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bioRxiv - Immunology 2023Quote: ... Real-time quantitative PCR was run on the Eppendorf Mastercycler (SybrGreen) using the LUNA Universal qPCR Master Mix (New England Biolabs, Catalog # M3003). Primers used for real-time qPCR can be found in Supplementary Data 5.
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bioRxiv - Physiology 2023Quote: ... RT-qPCR (quantitative reverse transcriptase PCR) was then performed using a Luna® Universal One-Step RT-qPCR Kit (New England Biolabs; E3005) per manufacturer’s instructions and primers for the CaSeq1 gene of interest (IDT ...
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bioRxiv - Microbiology 2023Quote: ... at a final volume of 20 μL with 10 μL of Luna® Universal qPCR Master Mix (New England Biolabs, Frankfurt, Germany). Each primer was added at a final concentration of 300 nM ...
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bioRxiv - Microbiology 2021Quote: ... PCR reactions were carried out in a 20 µl volume containing Luna Universal qPCR Master Mix (New England Biolabs, Inc., Cat. no. NC1276266), F515 (GTGCCAGCMGCCGCGGTAA ...
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bioRxiv - Immunology 2022Quote: ... Viral RNA was quantified using a one-step RT qPCR reaction with the NEB Luna Universal Probe One-Step RT-qPCR (New England Biolabs, Ipswich, MA, USA) and the 2019-nCoV RT-qPCR primers and probe (E_Sarbeco)84 on a StepOnePlus RealTime PCR System (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Each sample was analyzed in duplicate 10-µl reactions containing 5 µl Luna Universal qPCR Master Mix (New England Biolabs, Ipswich, MA, USA), 2 µM of each primer ...