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Citations for New England Biolabs :
51 - 100 of 2182 citations for Transcription Factor SOX 10 SOX10 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... In vitro transcription reactions were treated with DNAse (NEB) for 1 hour at 37°C ...
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bioRxiv - Genomics 2023Quote: ... Further amplification was achieved by in-vitro transcription that was performed overnight using a high-yield in vitro transcription kit (NEB, cat. no. E2050S). Reverse transcription was then performed on the RNA template using Maxima H-Reverse Transcriptase (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... the column was loaded with 80 units of Factor Xa (New England Biolabs, P8010) in cleavage buffer ...
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bioRxiv - Biochemistry 2023Quote: ... activation was performed overnight at 4 °C with 5 μg of Factor Xa (NEB) per mg of protein ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by in vitro transcription (HiScribe T7 polymerase, NEB #E2040S), DNA degradation (TurboDNAse ...
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bioRxiv - Molecular Biology 2022Quote: ... T7 in vitro transcription was performed using HiScribe (NEB E2050S), using PCR-generated DNA as template ...
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bioRxiv - Cell Biology 2020Quote: ... and amplified by in vitro transcription (HiScribe, New England Biolabs). RNA products were fragmented using Ambion RNA Fragmentation Reagents (AM8740 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Reverse transcription was performed using M-MulV Reverse Transcriptase (NEB) with 250 ng of total RNA using gene specific primers for Figure S11 and using an oligo-T primer for Figure 4e ...
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bioRxiv - Biochemistry 2020Quote: ... in vitro transcription reactions were treated with DNAseI (NEB M0303L) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: ... and used for in vitro transcription (SP6 RNA polymerase, NEB). Deletions were made using two guide RNAs and a single-stranded DNA oligonucleotide repair template with a co-conversion strategy (34) ...
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bioRxiv - Developmental Biology 2020Quote: ... were used for reverse transcription using ProtoSciprt II RT (NEB). Synthesized cDNA was diluted five times in water ...
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bioRxiv - Cell Biology 2021Quote: ... Reverse transcription was performed using LunaScript RT SuperMix Kit (NEB). Oligonucleotides used for qRT-PCR are listed in Supp ...
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bioRxiv - Molecular Biology 2021Quote: ... in vitro transcription reactions were treated with DNAseI (NEB M0303L) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... Each transcription reaction consisted of 9 mM rNTPs (NEB N0466), 10 mM Dithiothreitol (Invitrogen) ...
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bioRxiv - Molecular Biology 2022Quote: ... T7 in vitro transcription was performed using HiScribe (NEB E2050S), using PCR-generated DNA as template ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was obtained using reverse transcription PCR (Yugong Biolabs, China). The reaction system was prepared according to the kit’s requirements (Yugong Biolabs ...
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bioRxiv - Bioengineering 2024Quote: ... Transcription reactions using the Hiscribe IVT kit (New England Biolabs), were assembled to a final volume of 20 μl ...
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bioRxiv - Immunology 2023Quote: ... The transcription products were then purified (New England Biolabs, T2050L) and the cap1 tructure was added for efficient translation and evading the cellular innate immune response (New England Biolabs ...
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bioRxiv - Molecular Biology 2019Quote: The purified PCR products were used as templates for in vitro transcription of antisense and sense ssRNA by using the HiScribe™ T7 In Vitro Transcription Kit (NEB #E2030S, New England BioLabs Inc, Ipswich, Massachusetts, USA). Finally ...
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bioRxiv - Biochemistry 2020Quote: ... The PURExpress in vitro transcription-translation system was purchased from NEB. GeneJet PCR clean-up kit was purchased from Thermo-Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... SgRNA are produced by T7 Hiscribe transcription kit (New England Biolabs) and purified by EZNA microelute RNA clean up kit (Omega biotek) ...
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bioRxiv - Molecular Biology 2021Quote: ... Reverse transcription reaction with 0.2 U/μL AMV Reverse Transcriptase (NEB) was performed at 42°C for 30 min using a dNTP/ddCTP mix at 0.25 mM each (final concentration) ...
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bioRxiv - Physiology 2021Quote: ... T7 RNA polymerase mediated in vitro transcription (NEB, Frankfurt, Germany, E2040S) was used for sgRNA-generation from the pSpCas9(BB)-2A-GFP (PX458 ...
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bioRxiv - Molecular Biology 2022Quote: ... Reverse transcription (RT) was performed with M-MuLV reverse transcriptase (NEB) according to manufacturer instructions with a short oligonucleotide to prevent annealing of the oligonucleotides on RNAs that lost a few nucleotides at the 3’ end during the migration (and would thus run faster in the reverse run ...
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bioRxiv - Plant Biology 2022Quote: Reverse transcription was performed using the LunaScript RT SuperMix (NEB E3010) and 1μg DNase-treated total RNA with incubation at 25ºC for 2 min ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by reverse transcription using Protoscript II (New England Biolabs, M0368L) and circularization of cDNA using CircLigase ssDNA Ligase (Epicenter ...
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bioRxiv - Immunology 2023Quote: sgRNAs were produced using the transcription T7 high yield kit (NEB) and purified with the EZNA micro-elute RNA clean up kit (Omega Bio-tek) ...
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bioRxiv - Cancer Biology 2024Quote: ... The transcription products were purified with Monarch RNA Cleanup Kit (NEB) followed by polyadenylation using Poly(U ...
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bioRxiv - Microbiology 2024Quote: ... and reverse transcription was performed with ProtoScript II RT enzyme (NEB) using barcode primers (Supplementary Table S1) ...
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bioRxiv - Bioengineering 2022Quote: ... Then CaCl2 was added to 2mM and 1 µL (about 1 µg) of Factor Xa (NEB) was added per 50 µg of protein and the samples were left at RT for 20-24h ...
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bioRxiv - Molecular Biology 2021Quote: ... Customized PURExpress reconstituted systems lacking all amino acids and release factors (Δaa, ΔRF123) (New England Biolabs) (Fig ...
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bioRxiv - Neuroscience 2022Quote: In vitro transcription was performed using HiScribe Quick T7 kit (NEB E2050S) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... In vitro transcription was performed using HiScribe Quick T7 kit (NEB E2050S) following the manufacturer’s directions ...
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bioRxiv - Molecular Biology 2021Quote: ... digestion and then in vitro transcribed using HiScribe T7 Transcription Kit (NEB). The RNA transcripts were extracted using Trizol (Thermo ...
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bioRxiv - Molecular Biology 2020Quote: The 401 nt mRNA fragment was produced by in vitro transcription (NEB, HiScribe™ T7 High Yield RNA Synthesis Kit ...
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bioRxiv - Molecular Biology 2020Quote: ... Transcription reactions were carried out using SP6 polymerase (New England Biolabs, USA) for 1.5 hours at 37 °C and then added directly to nucleased RRL in the presence of radioactive 35S-methionine for 20 min at 32°C ...
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bioRxiv - Genomics 2020Quote: ... Reverse transcription was carried out using Protoscript II reverse transcriptase (NEB #M0368L) with oligo-dT priming according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... and reverse transcription performed using LunaScript RT SuperMix (E3010, New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... the purified PCR product was used for in vitro transcription (NEB, E2040S) followed by reverse transcription (Thermo Fisher ...
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bioRxiv - Genetics 2021Quote: ... Reverse transcription was carried out using ProtoScript II enzyme (New England Biolabs) and random 15-mer primers ...
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bioRxiv - Genetics 2020Quote: ... Total RNA was subjected to Oligo dT reverse transcription (NEB WarmStart RTx) and qPCR performed (NEB Luna qPCR MasterMix ...
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bioRxiv - Bioengineering 2020Quote: ... the SARS-CoV-2 RNA was obtained by in vitro transcription (NEB, HiScribe™ T7 High Yield RNA Synthesis Kit ...
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bioRxiv - Systems Biology 2021Quote: ... Reverse transcription was performed using Protoscript II reverse transcriptase (New England Biolabs) and oligo-dT primers (New England Biolabs) ...
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bioRxiv - Neuroscience 2023Quote: ... and in vitro transcription was performed with T7 RNA polymerase (NEB; M0251) and a digoxigenin RNA labelling kit (Roche ...
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bioRxiv - Evolutionary Biology 2023Quote: ... WMISH probes were generated via a SP6 or T7 transcription kit (NEB) and prospectively labelled with either Fluorescein or DIG-UTP ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA synthesis was achieved using the M-MuLV Reverse Transcription kit (NEB), according to manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2023Quote: ... The cDNA was synthesized using Proto Script II reverse transcription kit (NEB) and real-time PCR reactions were performed using Quant Studio 3 (Applied Biosystems ...
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bioRxiv - Microbiology 2023Quote: Reverse transcription was done using DNase-treated RNA using MMLV RT (NEB) according to the manufacture protocol ...
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bioRxiv - Immunology 2023Quote: ... Reverse transcription was carried out using LunaScript RT (New England Biolabs E3010) per manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... Reverse transcription was performed by the ProtoScript® II reverse transcriptase (NEB) using random primers ...