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Citations for New England Biolabs :
1 - 50 of 2182 citations for Transcription Factor SOX 10 SOX10 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... rabbit anti-Sox-2 (New England Biolabs), and rabbit anti-NANOG (New England Biolabs) ...
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bioRxiv - Neuroscience 2023Quote: SOX10 (89356, NEB, 1:400), sox10 zebrafish (AB229331 ...
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bioRxiv - Molecular Biology 2020Quote: ... and 3 μL of factor mix (with RNA polymerase, and transcription/translation factors in 10 mM Mg2+) from the PURExpress® Δ Ribosome Kit (New England Biolabs). The reaction buffer was based on Shimizu et al ...
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bioRxiv - Microbiology 2022Quote: ... SOX was then subcloned into pFastBac HTD vector using restriction sites BamHI and SalI (New England Biolabs). This vector was modified to carry a GST affinity tag and PreScission protease cut site as described (25) ...
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bioRxiv - Evolutionary Biology 2021Quote: Mutagenesis of the binding sites for the predicted Caudal and DEAF-1 transcription factors was performed sequentially with the Q5 Site-Directed Mutagenesis kit (New England Biolabs) taking as a template the pGreenRabbit vector containing the FBti0019386 sequence following manufacturer’s instructions (25) ...
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bioRxiv - Bioengineering 2024Quote: ... This fragment was cloned into a lentiviral expression plasmid we have previously described (31) encoding the Notch1 core linked to the tTA transcription factor using NEBuilder HiFi DNA Assembly (New England Biolabs). The resultant plasmid encoded constitutive expression of CII-synNotch receptor from an EF1α promoter and production of the puromycin N-acetyl-transferase transgene via the constitutive PGK promoter for antibiotic selection ...
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bioRxiv - Molecular Biology 2022Quote: ... 10 μL of reverse transcription products were then PCR amplified using Phusion polymerase (NEB) and T7_fwd and T7_rev (11 cycles ...
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bioRxiv - Biophysics 2022Quote: ... 1 µL factor mix (NEB), 250 nM pre-incubated 50S ribosomal subunits ...
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bioRxiv - Biophysics 2022Quote: ... 2 µL factor mix (NEB), 250 nM pre-incubated 50S subunit ...
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bioRxiv - Biophysics 2023Quote: ... 1 μL factor mix (NEB), 250 nM pre-incubated 50S subunit ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1 μL factor mix (NEB), 250 nM pre-incubated 50S ribosomal subunits ...
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bioRxiv - Molecular Biology 2021Quote: ... for 12 h at 4 °C with 10 μg of Factor Xa protease (New England Biolabs, Hitchin, UK) per 1 g of mitochondria ...
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bioRxiv - Biophysics 2020Quote: ... Factor Xa protease (New England Biolabs) was added to the MT solution at a molar ratio of 1 ...
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bioRxiv - Plant Biology 2021Quote: ... Factor Xa (P8010S, New England BioLabs) or TEV protease (P8112S ...
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bioRxiv - Biochemistry 2023Quote: ... 20 µg factor Xa protease (NEB) and 5 mM CaCl2 with inversion at 10 °C overnight ...
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bioRxiv - Bioengineering 2020Quote: ... The post-transcription reaction mixture was incubated with 10 units of DNase I (New England Biolabs) at 37°C for 1 hour to remove the gblock template then purified using a RNA spin column (Zymo Research) ...
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bioRxiv - Microbiology 2022Quote: ... Parallel T7 reactions performed at the same time used 1.25 μg XbaI linearised pT7HCV(5’+)341N79wt and pT7HCV(5’+)341N79ko as templates in a 25μl final reaction volume containing 2.5μl 10 x T7 transcription buffer (NEB), 0.6μl RNaseOUT ...
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bioRxiv - Biochemistry 2020Quote: ... 10 µCi/µl) by in vitro (run off) transcription driven by T7 RNA polymerase (New England Biolabs), Next ...
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bioRxiv - Molecular Biology 2024Quote: dsRNA was generated by in vitro transcription (NEB T7 E2050s transcription kit) from PCR products generated by the following forward and reverse primers (separated by comma):
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bioRxiv - Developmental Biology 2023Quote: ... Transcription reaction volume was reduced to 10 µl and 0.5 units of thermostable inorganic pyrophosphatase (New England Biolabs) was included in the reaction ...
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bioRxiv - Cell Biology 2021Quote: All dox-inducible factors were generated by cloning the open reading frame of each factor into the pMINI vector (NEB) and then restricted with EcoRI or MfeI and inserted into the FUW-TetO expression vector ...
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A genome-scale CRISPR interference guide library enables comprehensive phenotypic profiling in yeastbioRxiv - Genomics 2020Quote: ... Reverse transcription was carried out using 10 ng of purified RNA in a reaction with ProtoScript II (NEB M0368S) using 2.0 pmol NI-1032 as a gene-specific primer (Table S1) ...
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bioRxiv - Microbiology 2022Quote: ... Reverse transcription of 10 µl RNA was performed using LunaScript RT Supermix Kit (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2021Quote: ... In vitro transcription (NEB E2040S) was carried out according to the manufacturer’s instructions using 100 ng of DNA template ...
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bioRxiv - Genomics 2023Quote: ... in vitro transcription (NEB E2040S) at 42°C for 8 hours in the presence of RNasin Ribonuclease Inhibitor (Promega N2111 ...
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bioRxiv - Genomics 2023Quote: ... performed reverse transcription (NEB, E3010L) and ran qPCR (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... 100 nM Ca2+ and 10 μM purified Calmodulin (CaM) in the coupled transcription/translation PURE system (New England Biolabs, USA), which contains a defined set of purified translation factors and E ...
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bioRxiv - Molecular Biology 2022Quote: ... 2.5 μl reverse transcription reactions or ∼10 ng genomic DNA were then subjected to PCR amplification by the high-fidelity Phusion polymerase (NEB) in 50 μl reactions using the primer pairs listed in Supplementary Table S1 and the following cycling program ...
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bioRxiv - Biochemistry 2023Quote: Transcription reactions were prepared at room temperature in 20 µL transcription buffer (40 µM Tris-HCl pH 8, 5 mM DTT, 10 mM MgCl2) with 2 mM NTP’s (NEB, N0450S) and 40 U/µL RNasin™ Plus (Promega ...
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bioRxiv - Immunology 2021Quote: ... In-vitro-transcription was carried out using the Hi-Scribe RNA transcription kit (New England BioLabs). Briefly ...
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bioRxiv - Systems Biology 2020Quote: ... Then in vitro transcription (NEB E2040S) followed by reverse transcription (Thermo Fisher EP7051 ...
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bioRxiv - Microbiology 2020Quote: ... the resin was treated with factor Xa protease (P8010L, NEB) overnight at 4°C ...
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Phosphorylation controls spatial and temporal activities of motor-PRC1 complexes to complete mitosisbioRxiv - Biochemistry 2023Quote: ... the MPB tag was cleaved overnight using Factor Xa (NEB) in dialysis buffer and loaded again on an MBP-Trap HP column ...
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bioRxiv - Molecular Biology 2019Quote: ... Reverse transcription was with SuperScript III (NEB), and subsequent PCR amplification of the cDNA was with Phusion (NEB) ...
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bioRxiv - Physiology 2020Quote: ... 5x transcription buffer (NEB, Hitchin, Hertfordshire, UK), 0.2 M dithiothreitol (DTT ...
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bioRxiv - Synthetic Biology 2021Quote: ... The transcriptions (1X template, 1X Homemade NEB Buffer ...
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bioRxiv - Biochemistry 2021Quote: ... MBP was cleaved from LH using Factor Xa (New England Biolabs) at a w/w ratio of 1% Factor Xa:LH ...
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bioRxiv - Zoology 2023Quote: Reverse transcription was conducted with 10 µL of eluted nucleic acids using the ProtoScript® II Reverse Transcriptase (New England Biolabs, Massachusetts, USA). For each sample ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by T7 in vitro transcription using the HiScribe T7 In Vitro Transcription Kit (New England Biolabs, Ipswich, MA) and purification with Trizol (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... In vitro transcription was performed with either HiScribe T7 ARCA mRNA kit or the HiScribe In Vitro Transcription Kit (NEB) according to the manufacturer’s recommendation ...
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bioRxiv - Microbiology 2023Quote: ... Transcription and fluorescent labelling of RNA in vitro transcription reactions were carried out using a T7 RNA transcription kit (HiScribe T7 High Yield; New England Biolabs) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: In vitro transcription reactions were carried out using a T7 RNA transcription kit (HiScribe T7 High Yield; New England Biolabs) with the following modifications ...
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bioRxiv - Genomics 2023Quote: ... A transcription reaction was then carried out on the purified and quantified dsDNA using the T7 HiScribe transcription kit (NEB). The T7 RNA Polymerase recognizes the T7 promoter region that seeds transcription of the adjacent 20-mer target sequence thus generating the targeting sgRNA for Cas9-mediated nicking of genomic DNA templates ...
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bioRxiv - Genomics 2020Quote: ... Reverse transcription was performed using Protoscript II (NEB) [4 µl 5x NEB FirstStrand buffer (NEB ...
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bioRxiv - Neuroscience 2020Quote: ... and a reverse transcription kit (NEB, Ipswich, MA) according to the manufacturer protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Reverse transcription was performed using ProtoScript II (NEB). qPCR reactions were assembled with Luna Master Mix (NEB ...
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bioRxiv - Molecular Biology 2019Quote: The purified PCR products were used as templates for in vitro transcription of antisense and sense ssRNA by using the HiScribe™ T7 In Vitro Transcription Kit (NEB #E2030S ...
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bioRxiv - Microbiology 2020Quote: ... Appropriate fractions were pooled and digested with factor Xa protease (New England Biolabs) in the presence of 2 mM CaCl2 at 4 °C overnight ...
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bioRxiv - Cell Biology 2020Quote: ... Transcription reactions were treated with RNase-free DNaseI (NEB) to digest the DNA template at 37°C for 1 hour ...
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bioRxiv - Molecular Biology 2023Quote: ... Transcription reactions were carried out with SP6 polymerase (NEB) for 1 hour at 37°C ...