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Citations for New England Biolabs :
351 - 400 of 2182 citations for Transcription Factor SOX 10 SOX10 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... This amplicon was used as a template for synthesis of capped and poly(A)-tailed RNA using the T7 HiScribe in vitro transcription kit (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: Reverse transcription and amplification for figure 2 was performed using OneTaq One-Step RT-PCR Kit from NEB (cat. # E5315S). Both the OneTaq One-Step RT-PCR Kit and Luna Universal One-step RT-qPCR Kit (cat ...
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bioRxiv - Molecular Biology 2020Quote: ... and the CMV promoter was removed to prevent aberrant transcription by digesting the plasmid with ClaI-HF and BamHI-HF (NEB), gel extracting ...
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bioRxiv - Synthetic Biology 2019Quote: Immobilized toeholds strands bound on the magnetic beads were mixed with 30 µL of in vitro transcription buffer (NEB, E2050) containing 2 µL of T7 RNA Polymerase Mix and ATP ...
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bioRxiv - Biochemistry 2019Quote: ... complexes were eluted for 30 min at 25° with rotation in 40 μl of the Transcription Buffer with 60 units of either SspI-HF or PstI-HF (New England Biolabs) as indicated (see Fig ...
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bioRxiv - Microbiology 2019Quote: ... Reverse transcription was performed for 5 hours at 42 °C using 100 U of ProtoScript® II Reverse Transcriptase (NEB), 10 U of RNase Inhibitor (Murine ...
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bioRxiv - Molecular Biology 2019Quote: ... and set up in an overnight in vitro transcription reaction (HiScribe T7 Quick High Yield RNA Synthesis kit, New England BioLabs). The resulting RNA was purified using Silane beads (Life Technologies ...
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bioRxiv - Bioengineering 2019Quote: ... and 21 µg of gRNA transcripts produced by in vitro transcription of oligonucleotides (HiScribeTM T7 High Yield RNA Synthesis kit from NEB). Plugs are then treated overnight at 50°C with 10 µL of proteinase K ...
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bioRxiv - Immunology 2021Quote: The DNA sequences of B.1.351 and B.1.617 SARS-CoV-2 spikes for the mRNA transcription and pseudovirus assay were synthesized as gBlocks (IDT) and cloned by Gibson Assembly (NEB) into pcDNA3.1 plasmids ...
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bioRxiv - Biochemistry 2020Quote: DNA templates for in vitro transcription were amplified by PCR using custom DNA primers (IDT) and Phusion Hot Start polymerase (New England BioLabs). 2.5 mL transcription reactions were assembled using a 1000 μL PCR reaction ...
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bioRxiv - Bioengineering 2021Quote: Sense RNA fragments and and circular adRNA were made by in vitro transcription using the HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB) as per the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... were synthesized using a T7 RNA polymerase in vitro transcription with the HiScribe T7 ARCA mRNA Kit (with tailing) (NEB). The mRNAs were purified using a Monarch RNA Cleanup Kit (NEB) ...
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Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA-protein InteractionsbioRxiv - Biochemistry 2020Quote: ... Transcription reactions were set up with RNA NTPs (5 mM each ATP, CTP, UTP, 9 mM GTP) (NEB, Ipswitch, MA), 0.004 U/µL Thermostable Inorganic Pyrophosphatase (NEB ...
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bioRxiv - Plant Biology 2021Quote: ... 1µg of purified DNA was used for in-vitro transcription following the manufacturer instructions (HiScribe T7 High Yield RNA Synthesis Kit, NEB). Purified non-crosslinked chromatin obtained from five grams of MARS RNAi line 1 seedlings were resuspended in 1 mL of nuclei lysis buffer and split into five tubes ...
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bioRxiv - Plant Biology 2021Quote: ... the 560 bp region upstream of the transcription start site was amplified using Q5 high fidelity DNA polymerase (NEB, England) and ligated into EcoRV digested SK+ cloning vector ...
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bioRxiv - Microbiology 2021Quote: ... The DNA depleted RNA was used as the template for reverse transcription performed with Moloney murine leukemia virus (MMLV) reverse transcriptase (M0253, NEB). The cDNA samples were then diluted 1:4 in water and used in quantitative real-time PCR with genespecific primers using SsoAdvanced Universal Sybr green Supermix (1725271 ...
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bioRxiv - Microbiology 2021Quote: ... Five hundred nanograms of linearized pBSKS-dhfr vector was used as a DNA template for in vitro transcription with T7 RNA Polymerase (New England Biolabs) at 37 °C for 4 h in a 20 μl reaction mixture ...
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bioRxiv - Immunology 2022Quote: ... In vitro transcription (IVT) was performed at 37°C for 13-16h with the HiScribe T7 RNA Polymerase kit (NEB). The remaining DNA was digested by Turbo DNase I (Life Technologies ...
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bioRxiv - Genomics 2022Quote: ... Then we performed the bead-on T7 transcription reaction by HiScribe™ T7 High Yield RNA Synthesis Kit (NEB E2040S). The amplified RNAs were processed to the PCR-cDNA sequencing kit (nanopore) ...
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bioRxiv - Genetics 2022Quote: A DNA template for in vitro transcription was generated by PCR using oligos TE122 and TE126 on plasmid pCT-TE2 and Phusion DNA polymerase (NEB) in HF buffer (Table 1) ...
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bioRxiv - Cell Biology 2022Quote: ... The assembled template was purified and subjected to in vitro transcription by T7 RNA polymerase using the Hiscribe T7 High Yield RNA Synthesis Kit (New England Biolabs). The reaction product was treated with DNase I ...
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bioRxiv - Systems Biology 2022Quote: ... coding sequences and terminators were assembled together into the transcription unit acceptor plasmid (POT1-ccdB) by Golden Gate assembly using Esp3I (NEB); these are low-copy centromeric plasmids with URA3 selection marker ...
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bioRxiv - Cell Biology 2022Quote: ... 400 ng to 1 μg of input RNA was used as a template for reverse transcription using Protoscript II First Strand cDNA Synthesis Kit (NEB) and random hexamers ...
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bioRxiv - Developmental Biology 2021Quote: ... Reaction products were used as a template for the in vitro transcription (HiScribe T7 High Yield RNA Synthesis Kit, England Biolabs) and purified ...
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bioRxiv - Microbiology 2021Quote: In vitro RNA capping was achieved using RNAs produced by in vitro transcription as substrate and the Vaccinia capping system (NEB). The 37-mer (50 μM ...
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bioRxiv - Microbiology 2021Quote: ... NA and NP RNA were synthesized using a HiScribe T7 in vitro transcription kit (New England BioLabs, Ipswich, MA, USA) and incubated with 3D8 scFv purified protein (0.5 μg ...
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bioRxiv - Cell Biology 2022Quote: ... The purified DNA template was subjected to in vitro transcription by T7 RNA polymerase using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs). After being treated with DNase I (Takara Bio) ...
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bioRxiv - Bioengineering 2019Quote: ... with 3, 6, or 12 nM iSpinach DNA (IDT, USA, Ultramers) template in transcription buffer (1x RNAPol Reaction Buffer (NEB, No ...
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bioRxiv - Microbiology 2020Quote: ... The DNA depleted RNA was used as the template for reverse transcription performed with Moloney murine leukemia virus (MMLV) reverse transcriptase (M0253, NEB). The cDNA samples were then diluted 1:4 in water and used in quantitative real-time PCR with gene-specific primers using SsoAdvanced Universal Sybr green Supermix (1725271 ...
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bioRxiv - Developmental Biology 2020Quote: ... To make the sgRNA we linearized purified pDR274 containing the guide sequence with Dra1 and used a T7 RNA polymerase for in vitro transcription (NEB). The pMLM3613 plasmid encoding cas9 was used for in vitro transcription using the SP6 mMessage mMachine Kit (Ambion ...
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bioRxiv - Molecular Biology 2019Quote: ... The purified DNA was used to generate the corresponding RNA by in vitro transcription using HiScribe T7 polymerase (NEB #E2040S). After purifying the RNA with carboxylate-modified magnetic beads ...
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bioRxiv - Molecular Biology 2020Quote: Linear DNA templates for in vitro transcription were generated from pNB1u or pOO2-GW plasmid by PCR using Phusion High-Fidelity DNA Polymerase (NEB), according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... The in vitro transcription of ayRhp1 capped mRNA (cRNA) was performed with HiScribe T7 ARCA mRNA kit (New England Biolabs) on SphI linearized pGEM-HE-ayRhp1 vector followed by column purification (RNeasy MiniElute Cleanup Kit ...
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bioRxiv - Microbiology 2021Quote: ... Transcription of pgRNA constructs were carried out using a Hiscribe™ T7 High Yield RNA Synthesis Kit (New England Biolabs), after linearization of the DNA plasmid using HindIII ...
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bioRxiv - Molecular Biology 2020Quote: ... Region B or Region C were used as templates for in vitro transcription by HiScribe T7 quick high yield RNA synthesis kit (NEB). The obtained RNA products were converted back to DNA oligo probes via reverse transcription (RT ...
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bioRxiv - Immunology 2020Quote: ... In vitro transcription of gRNAs from assembled oligonucleotides was conducted using the HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB). Injected clutches of embryos were validated to contain CRISPR mediated mutagenesis by a T7 endonuclease assay ...
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bioRxiv - Molecular Biology 2020Quote: ... Guide RNAs were cloned into a T7 promoter vector followed by in vitro transcription (HiScribe T7 High Yield RNA Synthesis Kit, New England BioLabs) and spin column purification (RNeasy ...
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bioRxiv - Biochemistry 2020Quote: DNA templates for in vitro transcription were amplified by PCR using custom DNA primers (IDT) and Phusion Hot Start polymerase (New England BioLabs). 2.5 mL transcription reactions were assembled using 1000 µL PCR reactions as template (∼0.2 µM template DNA) ...
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bioRxiv - Molecular Biology 2019Quote: ... tRNA containing plasmids were linearized using restriction digestion and tRNAs were in vitro transcribed overnight using the HiScribe T7 transcription kit (NEB) per the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ssRNA was transcribed directly from the PCR amplicon product in a reverse transcription reaction using a HiScribe T7 polymerase (NEB). The DNA template was then removed through treatment with DNase1 ...
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bioRxiv - Microbiology 2021Quote: The DNA template for T7 transcription was prepared by linearizing the T7 replicon plasmid (#453, Supplemental Table I) with the restriction enzyme SacII (NEB), followed by protease K treatment ...
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bioRxiv - Pathology 2021Quote: Reverse-transcription polymerase chain reaction (RT-PCR) was performed using the Luna Universal Probe One-Step RT-qPCR kit (NEB). Two gene targets were used for SARS-CoV-2 RNA detection ...
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bioRxiv - Biophysics 2021Quote: ... In vitro transcription on the DNA substrates was performed by using HiScribe T7 High Yield RNA synthesis kit (E2040S, New England BioLabs) for 120 min at 37 °C in the presence of 1 mM GTP ...
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bioRxiv - Immunology 2020Quote: ... The linearized plasmids were purified and utilized as templates in a T7 ARCA in vitro transcription reaction (New England Biolabs). The mRNA product was then purified using an Invitrogen Purelink RNA mini kit according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... and then served as the DNA template for in vitro transcription reactions using the HiScribe T7 High Yield RNA Synthesis Kit (Cat. No. E2040S, NEB). Template DNA was removed by the addition of DNase I (Cat ...
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bioRxiv - Microbiology 2022Quote: ... Strand-specific CHIKV RNA primers were chosen from previous work [54] to amplify nucleotides 1-1560 of the CHIKV genome and used in reverse transcription reactions with the LunaScript8482 RT SuperMix Kit (NEB), followed by qPCR using the 2x qPCRBIO SyGreen Blue Mix (PCRBIOSYSTEMS) ...
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bioRxiv - Biophysics 2022Quote: ... vulnificus adenine riboswitch aptamer domain by in vitro transcription of the corresponding DNA oligonucleotides (HiScribe T7 High Yield RNA Synthesis Kit, New England Biolabs; oligonucleotides ordered from Eurofins) ...
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bioRxiv - Bioengineering 2022Quote: ... Synthetic ORF1a target RNA fragment was produced from the corresponding cDNA fragment by in vitro transcription using the HiScribe T7 High Yield RNA Synthesis Kit (NEB), according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Anti-sense RNA probes were then generated by in vitro transcription using Sp6 polymerase and purified with the Monarch RNA Cleanup Kit (NEB). Primers and gene block sequences are provided in Supplementary Table 1 and 2 ...
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bioRxiv - Molecular Biology 2022Quote: ... after reverse transcription of pulled-down RNA and synthesis of a second strand (NEBNext mRNA second strand synthesis module (NEB)) ...