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Citations for New England Biolabs :
251 - 300 of 3908 citations for Thymidine Kinase 1 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: ... The reverse primers are phosphorylated using Polynucleotide kinase (NEB).
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bioRxiv - Cancer Biology 2021Quote: ... Insert oligonucleotides were phosphorylated using T4 polynucleotide kinase (NEB) at 37 °C for 1 hr and were annealed by heating to 95°C for 5 min and slowly cooled to room temperature ...
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bioRxiv - Pathology 2021Quote: ... then treated with Kinase-Ligase-DpnI (New England Biolabs) prior to heat shock transformation into chemically competent DH5α Escherichia coli cells (Table 1 ...
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bioRxiv - Molecular Biology 2020Quote: ... T4 polynucleotide kinase (New England Biolabs, with 1mM ATP) and Terminal exonuclease (buffer A ...
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bioRxiv - Genetics 2019Quote: ... Annealed oligos were phosphorylated by T4 polynucleotide kinase (NEB) for two hours at 37°C followed by 65°C for 20 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... T4 Polynucleotide kinase PNK 200 U/ml (NEB M0201L); DNA Polymerase I Large (Klenow ...
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bioRxiv - Cell Biology 2022Quote: ... Oligos were phosphorylated with T4 Polynucleotide Kinase (NEB M0201) at 37oC for 30 minutes ...
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bioRxiv - Plant Biology 2022Quote: ... Following phosphorylation with T4 polynucleotide kinase (New England BioLabs), the PCR products were ligated into expression vector pQE-1 (Qiagen ...
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bioRxiv - Molecular Biology 2022Quote: ... 5’ phosphorylated by T4 polynucleotide kinase (New England Biolabs), ligated to a blunt-end linear vector (typically also PCR products) ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were circularized with T4 kinase (NEB, M0201L) and T4 ligase (NEB ...
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bioRxiv - Cancer Biology 2023Quote: ... DpnI restriction enzyme and polynucleotide kinase were used (NEB). Some constructs were made using the Gateway cloning system (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... and 7 μl Polynucleotide Kinase (10 U/µl; NEB) in a total of 100 μl and incubation for 20 minutes at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... These primers were phosphorylated using T4 polynucleotide kinase (NEB) prior to PCR ...
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bioRxiv - Biochemistry 2023Quote: ... and human casein kinase II (New England BioLabs, #P6010).
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bioRxiv - Biochemistry 2023Quote: ... followed by KLD (Kinase, Ligase, and DpnI) digestion (NEB) by using primers deltaTatA_F (5’-GTGTTTGATATCGGTTTTAGC-3’ ...
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bioRxiv - Biophysics 2023Quote: ... and 10 U T4 polynucleotide kinase (New England Biolabs) in a final volume of 10 µL ...
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bioRxiv - Neuroscience 2023Quote: ... and were then phosphorylated using T4 polynucleotide kinase (NEB) according to the manufacturer’s manual ...
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bioRxiv - Biophysics 2023Quote: ... The sample was phosphorylated using protein kinase A (NEB) and then further purified on size exclusion chromatography.
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bioRxiv - Cancer Biology 2023Quote: ... RNA was 3’ dephosphorylated using T4 Polynucleotide Kinase (NEB) and preadenylated linkers were ligated to the RNA fragments using T4 RNA Ligase 2 K277Q (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by 5’ phosphorylation with T4 Polynucleotide Kinase (NEB) and self-ligation with T4 DNA Ligase (NEB).
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bioRxiv - Molecular Biology 2023Quote: ... and T4 poly nucleotide kinase (New England Biolabs, M0201L) and then ligated with 3ʹ RNA linker (with sample barcodes ...
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bioRxiv - Biochemistry 2024Quote: ... and 10 U T4 polynucleotide kinase (New England Biolabs) in a final volume of 10 µL ...
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bioRxiv - Developmental Biology 2023Quote: ... and end-labeled using T4 polynucleotide kinase (M0201, NEB) and [γ-32P] ATP (NEG002A250UC ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant Cas9 protein (NEB) and sgRNA prepared by in vitro transcription were added to the mixture and incubated at 37℃ for ∼ 4h to remove rRNA ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 μl of 100 μM SARS_139 and SARS_140 primers were mixed with 1 μl T4 Polynucleotide kinase (PNK; NEB) in 1x PNK reaction buffer (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: ... The DNA probes (Extended Table 1) at 500 nM were 5’-[32P]-labeled using T4 Polynucleotide Kinase (NEB) and hybridized to the membrane overnight at 37°C in PerfectHyb™ Plus Hybridization Buffer (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... 20 pmol of the obtained RNA were then radiolabeled by incubation with 1 U of Polynucleotide Kinase (NEB) and 20 μCi of 32P-γATP for 1 h at 37°C in a 20 μL reaction volume ...
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bioRxiv - Microbiology 2022Quote: ... Immunoprecipitation was performed overnight under rotation at 4 using 1/100 T7RNA antibody (Biolabs CB MAB-0296MC) and antiflag (Sigma F1804 and F3165) ...
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bioRxiv - Biophysics 2021Quote: ... The E1371Q samples were phosphorylated using protein kinase A (NEB). The wild-type sample was de-phosphorylated using λ-phosphatase ...
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bioRxiv - Genetics 2021Quote: ... and 10 U polynucleotide kinase (New England Biolabs, Beverly, MA) as instructed by the manufacturer ...
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bioRxiv - Microbiology 2019Quote: ... and then modified using T4 Polynucleotide Kinase (New England Biolabs), which removes the 3’-phosphate and adds 5’-phosphates ...
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bioRxiv - Genetics 2019Quote: ... followed by treatment with T4 polynucleotide kinase (New England BioLabs) as described previously53 ...
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bioRxiv - Cancer Biology 2020Quote: ... annealed oligonucleotides were phosphorylated with T4 polynucleotide kinase (NEB, M0201S) and cloned into BsmBI-linearized ...
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bioRxiv - Genomics 2019Quote: ... the RNA products were treated with Polynucleotide Kinase (PNK, NEB) at 37°C for 30 min.
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bioRxiv - Molecular Biology 2020Quote: ... Telomere probe was labeled using T4 polynucleotide kinase (NEB M0201) and <ι>γ-P32-ATP (Perkin Elmer NEG035C ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated with 8 μl of T4 Polynucleotide Kinase (NEB) and 2 μl of 10 mM ATP for 30 min at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: ... the oligos were phosphorylated by T4 Polynucleotide Kinase (M0201S, NEB) at 37°C for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... followed by phosphorylation with T4 polynucleotide kinase (New England Biolabs). The vector pPEPZ-sgRNAclone was digested with BsmBI and carefully purified by gel extraction to ensure removal of the mCherry fragment ...
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bioRxiv - Neuroscience 2020Quote: ... Ca2+/calmodulin-dependent protein kinase II (CaMKII, New England Biolabs).
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bioRxiv - Cancer Biology 2022Quote: ... and 1.5 µ l of T4 Polynucleotide Kinase (NEB, M0201L). Following incubation ...
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bioRxiv - Neuroscience 2022Quote: ... and 1x NEB-uffer for Protein Kinases (New England Biolabs). The samples were then subjected to protein precipitation ...
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bioRxiv - Microbiology 2021Quote: ... and 0.4 μl of T4 polynucleotide kinase (New England Biolabs). The THN gene was amplified with the phosphorylated primers MR161-MR162 using Phusion Flash High-Fidelity PCR Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: The annealing reaction was performed using T4 polynucleotide kinase (NEB) under the following conditions ...
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bioRxiv - Biochemistry 2019Quote: Kinases were treated with 0.5µL lambda phosphatase (New England Biolabs) per 1µg protein for 1 hour at 30°C in PMP buffer (New England Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... RNA fragments were further dephosphorylated using T4 polynucleotide kinase (NEB) for 1h at 37°C followed by heat inactivation for 10min at 75°C ...
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bioRxiv - Microbiology 2019Quote: ... The strands were end phosphorylated by T4 polynucleotide kinase (NEB) and annealed ...
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bioRxiv - Microbiology 2019Quote: ... The strands were end phosphorylated by T4 polynucleotide kinase (NEB), annealed ...
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bioRxiv - Biochemistry 2021Quote: ... using a standard T4 polynucleotide kinase labeling protocol (M0201L, NEB). Each binding reaction (10 μL sample volume ...
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bioRxiv - Biochemistry 2021Quote: 5’ phosphorylation of micro HHrz RNA with polynucleotide kinase (NEB) as done following manufacturer’s directions ...
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bioRxiv - Immunology 2020Quote: ... the remaining RNA was T4 Polynucleotide Kinase (New England Biolabs) treated ...