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Citations for New England Biolabs :
501 - 550 of 3908 citations for Thymidine Kinase 1 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... Top and bottom guide RNAs were annealed with T4 Polynucleotide Kinase (3’ phosphatase minus, NEB) and T4 DNA Ligase Buffer (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... oligonucleotides were 5-end labeled with 32P-γATP using T4 polynucleotide kinase (New England Biolabs). Supercoiled pUC19 plasmid dsDNA was prepared by a method that did not involve DNA denaturation (Clewell and Helinski ...
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bioRxiv - Biochemistry 2020Quote: 100 ng of purified tsRNAs were treated with 0.5 U/µL T4 polynucleotide kinase (NEB) and 166 µM ATP (NEB ...
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bioRxiv - Synthetic Biology 2020Quote: ... if necessary (crRNA arrays) also phosphorylated with T4 polynucleotide kinase (New England Biolabs, MA, USA) and ligated with T4 DNA ligase in previously BsmbI digested vectors.
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bioRxiv - Genomics 2021Quote: ... PCR fragments were labeled using 32P-γ-ATP with T4 polynucleotide kinase (New England, Biolabs). Protein–DNA complexes were incubated for 1h at room temperature in binding buffer containing 25 mM Tris pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: ... which was purchased from Integrated DNA Technologies and end-labeled with T4 polynucleotide kinase (NEB) and γP32-ATP (6000 Ci/mmol ...
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bioRxiv - Biochemistry 2021Quote: ... RNA substrates were 5’ end labeled using γ32P-ATP and T4 Polynucleotide Kinase (NEB, MA)38 ...
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bioRxiv - Biochemistry 2020Quote: ... and subsequently 5’32P-labeled using [γ-32P]-ATP and T4 polynucleotide kinase (New England Biolabs) according to the manufacturer’s protocols.
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bioRxiv - Biochemistry 2021Quote: RNA substrates (listed in Table S1) were either 5’ labelled by T4 polynucleotide kinase (NEB) and 5’ 32P-γ −ATP ...
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bioRxiv - Molecular Biology 2022Quote: ... size selected ribosome footprints were treated with T4 Polynucleotide Kinase (#M0201L, New England Biolabs (NEB)) followed by ligation to a DNA linker using T4 RNA ligase 2 ...
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bioRxiv - Molecular Biology 2020Quote: ... PLPs were ordered as large pools and phosphorylated with T4 Polynucleotide Kinase (New England Biolabs). Sequences of all oligonucleotides can be found in Supplementary Table 1-4 ...
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bioRxiv - Microbiology 2021Quote: ... primers MR161 and MR162 (Table 2) were phosphorylated with T4 Polynucleotide Kinase (New England Biolabs) at 37°C for 1 h in a 4.5 μl reaction volume with 10 μM primer ...
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bioRxiv - Cancer Biology 2022Quote: ... Phosphorylation of the 5’ end using T4 polynucleotide kinase (New England Biolabs, Ipswich, MA, M0201L) (iii ...
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Phospho-sRNA-seq reveals extracellular mRNA/lncRNA fragments as potential biomarkers in human plasmabioRxiv - Molecular Biology 2019Quote: ... synthetic and plasma RNA samples were pretreated with T4 polynucleotide kinase (NEB, Ipswich, MA, US) using an RNA input of 7 ul in a final reaction volume of 10 ul and following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... When needed purified RNA was treated with T4 polynucleotide kinase (PNK) (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Biochemistry 2021Quote: ... When needed purified RNA was treated with T4 polynucleotide kinase (PNK) (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Biochemistry 2021Quote: ... oligonucleotide annealing was carried out in a thermocycler in T4 polynucleotide kinase reaction buffer (NEB) / 50 mM potassium acetate by heating oligonucleotide mixtures to 95 °C for 5 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... The ends of the fragmented RNA were repaired by treatment with T4 Polynucleotide Kinase (NEB) and Calf intestinal alkaline phosphatase (NEB ...
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bioRxiv - Cell Biology 2022Quote: ... gRNA was phosphorylated by a T4 polynucleotide kinase according to product instructions (New England Biolabs). For the amplification of phosphorylated sgRNA it was incubated with 1 μl of forward and reverse primers (100 μM ...
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bioRxiv - Biophysics 2023Quote: ... the insert PCR fragment was phosphorylated with P4 polynucleotide Kinase (M0201, New England Biolabs, UK) and the destination PCR-fragment was DPN1 treated (R0176 ...
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bioRxiv - Genetics 2023Quote: ... Synthetic oligonucleotides were labeled with [γ 32P]-ATP and T4 polynucleotide kinase (New England Biolabs) at their 5’ end ...
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bioRxiv - Cell Biology 2023Quote: ... Sense and antisense oligos were annealed with T4 Polynucleotide Kinase (PNK) (New England Biolabs; #M0201) and ligated with the digested cutting plasmid with T4 DNA ligase (New England Biolabs ...
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bioRxiv - Synthetic Biology 2023Quote: ... To prepare the TEV substrate two oligos were annealed using T4 Polynucleotide Kinase (NEB#M0201S) and T4 DNA ligase buffer (NEB#B0202S) ...
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bioRxiv - Molecular Biology 2023Quote: ... Small RNAs were 3’ dephosphorylated and 5’ phosphorylated with T4 polynucleotide kinase (T4PNK, NEB, #M0201S), and purified with RNA Clean and Concentrator-5 (Zymo Research ...
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bioRxiv - Microbiology 2023Quote: ... The 5’ ends were radiolabelled with 20 μCi γ32P-ATP using T4 polynucleotide kinase (NEB) and separated from free nucleotides using a MicroSpin G-50 column (Cytiva) ...
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bioRxiv - Synthetic Biology 2023Quote: ... the complementary oligos were phosphorylated using T4 polynucleotide kinase from New England Biolabs (NEB, USA) first and then ligated to pVT36b using Golden Gate Assembly (Engler et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... Purified fragments were dephosphorylated at their 3′ ends with T4 polynucleotide kinase (New England Biolabs) at 37 °C for 1 h and ligated to a 3′ adaptor sequence using T4 RNA ligase 2 (truncated K227Q ...
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bioRxiv - Molecular Biology 2023Quote: ... Probes were synthesized as ssDNA oligos and 5’ 32P-radiolabeled with T4 polynucleotide-kinase (NEB) and ψ-32P-ATP (6000 Ci/mmol ...
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Structural and mechanistic insights into disease-associated endolysosomal exonucleases PLD3 and PLD4bioRxiv - Biochemistry 2023Quote: Seventy picomoles of oligonucleotide (40T) was labelled with polynucleotide kinase (PNK) in PNK buffer (NEB) in the presence of γ32P-ATP (Perkin-Elmer ...
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bioRxiv - Biochemistry 2023Quote: ... 50 ng of GLORI-treated RNA sample was repaired using T4 Polynucleotide Kinase (NEB, #M0201S). Pre-adenylated 3’-adapter and 5’-adapter were sequentially ligated to the RNA using T4 RNA Ligase 2 ...
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bioRxiv - Biophysics 2023Quote: ... each primer pair was annealed and consequently phosphorylated with T4-polynucleotide kinase (T4-PNK; NEB). Restriction digestion of the pLentiCRISPRv2 vector was performed using FD-Esp3I (Thermo ...
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bioRxiv - Plant Biology 2024Quote: ... dephosphorylated F4 (5 µM) was incubated with γ-[32P]-ATP and T4 polynucleotide kinase (NEB) for 45 min at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... and the PCR products were subsequently 5’ phosphorylated using T4 polynucleotide kinase (New England Biolabs) and self-ligated by T4 DNA ligase (New England Biolabs ...
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bioRxiv - Genomics 2021Quote: ... The purified recombinant protein or commercial M.EcoGII (NEB, M0603S) was diluted with coating buffer (0.05 M NaHCO3 buffer ...
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bioRxiv - Biochemistry 2021Quote: ... This experiment was further repeated with recombinant Histones (NEB) (0.5µg ...
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bioRxiv - Genetics 2020Quote: ... MEFs were protein transfected with recombinant RNase H (NEB) using Project Reagent Transfection Kit (Thermo ...
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bioRxiv - Molecular Biology 2023Quote: ... a combination of both recombinant RBPMS and BSA (NEB). Splicing reactions were incubated for the 3 hr ...
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bioRxiv - Molecular Biology 2023Quote: ... 20pmol of recombinant histone H3.1-H4 tetramer (NEB, M2509S) were immediately added to the tube ...
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A crucial role for dynamic expression of components encoding the negative arm of the circadian clockbioRxiv - Biochemistry 2023Quote: ... Recombinant human Histone H2A (New England BioLabs, Catalog # M2502S) or H4 (New England BioLabs ...
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bioRxiv - Microbiology 2022Quote: ... Institut Jacques Boy), recombinant human IL-2 (rhIL2, 10 U/ml, Miltenyi) and DNase (1 U/ml, New England Biolabs), washed and enumerated ...
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bioRxiv - Cell Biology 2024Quote: ... and incubated with 200 µM ATP with ot without 1 µl of recombinant PKA catalytic subunit (PKAc; New England Biolabs) in manufacturer-supplied reaction buffer at 30° C for 30min with agitation ...
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bioRxiv - Biophysics 2021Quote: ... Fragmented 5’-OH sites were phosphorylated by treatment with 5 units of T4 polynucleotide kinase (NEB) for 1 hour at 37 °C ...
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bioRxiv - Genomics 2020Quote: ... 5mM DTT) and phosphorylated the 5’-ends with 25 units of T4 Polynucleotide Kinase (NEB #M0201) at 37°C for 15 minutes ...
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bioRxiv - Microbiology 2019Quote: ... adjacent primers containing the mutation of interest were 5′-phosphorylated using T4 polynucleotide kinase (NEB, M0201S) using the buffer from the T4 DNA ligase ...
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bioRxiv - Genetics 2019Quote: ... and phosphate groups added to the 5’ends using T4 Polynucleotide Kinase (New England Biolabs, M0201S) by first incubating for 30 minutes without ATP and then 30 min with ATP to remove and add the phosphate group ...
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bioRxiv - Microbiology 2021Quote: ... mutagenic oligonucleotides for each CDR were mixed and phosphorylated by T4 polynucleotide kinase (New England BioLabs) in 70mM Tris–HCl (pH7.6) ...
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bioRxiv - Cell Biology 2021Quote: ... The complexes on the beads were incubated with 40 units T4 Polynucleotide kinase (New England Biolabs), first with P32 labelled ATP for 45 min ...
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bioRxiv - Molecular Biology 2022Quote: ... Annealed oligonucleotides containing the homology region were phosphorylated with T4 polynucleotide kinase (New England Biolabs, M201) and then ligated into the Bbs1 site of the vectors pX335 and pKN7 (Appendix Tables S1 ...
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bioRxiv - Molecular Biology 2020Quote: ... 1µL of each Fw and Rv were annealed and phosphorylated with T4 Polynucleotide Kinase (PNK, NEB) using the following program ...
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bioRxiv - Molecular Biology 2019Quote: ... a master mix was prepared containing 2 µl 10x T4 polynucleotide kinase buffer without ATP (NEB) and 1 µl murine RNase inhibitor per sample and 3 µl were added to each sample together with 2 µl truncated T4 polynucleotide kinase (#M0201 ...