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Citations for New England Biolabs :
201 - 250 of 3908 citations for Thymidine Kinase 1 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... RNA was dephosphorylated using T4 Polynucleotide Kinase (NEB). The reverse transcriptase linker was then ligated with T4 RNA Ligase 2 truncated (NEB ...
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bioRxiv - Biophysics 2022Quote: ... λ DNA was phosphorylated with T4 polynucleotide kinase (NEB), ligated to PCR-Bio/Nt.BspQI with T4 DNA ligase (NEB ...
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bioRxiv - Cell Biology 2022Quote: ... were added to 1x protein kinase buffer (NEB) supplemented with 200 μM “cold” ATP (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... with 10 U of T4 Polynucleotide kinase (NEB) for 1 h at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... 0.5 units/µL T4 polynucleotide kinase (M0201, NEB), 0.12 units/µL T4 DNA polymerase (M0203 ...
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bioRxiv - Microbiology 2022Quote: ... containing T4 polynucleotide kinase (PNK; 0.2 μl; NEB) in a total volume of 10 μl to obtain double-stranded DNA flanked by XbaI and HindIII overhangs ...
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bioRxiv - Microbiology 2022Quote: ... The oligos were phosphorylated (T4 Polynucleotide Kinase; NEB) and annealed in a thermal cycler according to the following conditions ...
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bioRxiv - Microbiology 2022Quote: ... using the T4-polynucleotide kinase (New England Biolabs). For EMSA assays ...
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bioRxiv - Molecular Biology 2023Quote: ... dephosphorylated by T4 polynucleotide kinase (New England Biolabs), ligated to linkers by T4 RNA Ligase 2 ...
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bioRxiv - Bioengineering 2023Quote: T4 Polynucleotide Kinase (NEB cat. no. M0201S/L)
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bioRxiv - Molecular Biology 2023Quote: ... 40 units T4 polynucleotide kinase (New England Biolabs) for 45 min at 37°C in the manufacturer’s buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... dephosphorylated by T4 polynucleotide kinase (New England Biolabs), ligated to preadenylated linkers containing the T7 promoter region by T4 RNA ligase 2 ...
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bioRxiv - Molecular Biology 2023Quote: ... were used together with T4 polynucleotide kinase (NEB). Biotinylated ligation products were pulled down using streptavidin beads (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... followed by Casein Kinase II (New England Biolabs) in 1X PK buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... phosphorylation with T4 Polynucleotide Kinase (New England BioLabs), and circularization with an NEB Quick Ligation Kit (New England BioLabs) ...
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bioRxiv - Cancer Biology 2022Quote: ... and T4 polynucleotide kinase (New England Biolabs, #M0201S), and then ligating annealed sgRNAs and pre-digested and purified LentiCRISPRv2E vector with Quick Ligation Kit (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 units of T4 polynucleotide kinase (NEB, M0201S), 0.09 nM dNTPs and 0.045 μg/μL of BSA at 12°C for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: Purified RNA was treated with polynucleotide kinase (NEB) to prepare 5’ and 3’ ends for library construction as described for LORAX-seq.
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bioRxiv - Biophysics 2023Quote: ... and phosphorylated with protein kinase A (PKA) (NEB) for 1 hour at 20 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1.5ul of T4 Polynucleotide Kinase (NEB, M0201L). Sample was incubated at 37°C for 1 h and then inactivated by addition of EDTA at a final concentration of 20mM ...
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bioRxiv - Plant Biology 2023Quote: ... and 5′ phosphorylated with T4 Polynucleotide Kinase (NEB). The fragments were mixed with Golden Gate cloning adaptors (5′ adapter ...
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bioRxiv - Cancer Biology 2023Quote: ... 3 µl of T4 polynucleotide kinase (NEB, M0201L) and 4 µl of terminal deoxynucleotidyl transferase (Enzymatics ...
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bioRxiv - Genomics 2023Quote: ... and T4 Polynucleotide Kinase (New England Biolabs, M0201L). 1uL of 1uM annealed sgRNA oligos were then cloned into 30ng Lenti-sgRNA(MS2)-puro constructs (Addgene #73797 ...
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bioRxiv - Cancer Biology 2024Quote: ... and T4 polynucleotide kinase (New England BioLabs (NEB)) ...
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bioRxiv - Cancer Biology 2024Quote: ... and T4 polynucleotide kinase (New England BioLabs (NEB)) ...
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bioRxiv - Cancer Biology 2024Quote: ... and T4 polynucleotide kinase (T4 PNK, NEB, M0201) was used to add a phosphate group at the 5′ ends ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1.5ul of T4 Polynucleotide Kinase (NEB, M0201L). Samples were incubated at 37°C for 1h and then inactivated by addition of EDTA at a final concentration of 20mM ...
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bioRxiv - Genomics 2024Quote: ... 15 U T4 polynucleotide kinase (New England Biolabs), 5 U Klenow DNA polymerase (Roche) ...
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bioRxiv - Cancer Biology 2024Quote: ... After dephosphorylation with T4 Polynucleotide Kinase (NEB, #M0201S) the adapter Linker-1 (5’ rAppCTGTAGGCACCATCAAT/3ddC/ 3’ ...
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bioRxiv - Molecular Biology 2019Quote: ... 100 pmol of the oligonucleotides 5’-ATTGTCATACCGATCCCAATTCGA-3’ and 5’-AAACTCGAATTGGGATCGGTATGAC-3’ were phosphorylated for 30 min at 37 °C using 1 mM ATP and 1 unit polynucleotide kinase (NEB) in the buffer supplied by the manufacturer and then hybridized in a thermocycler (5 min 95 °C ...
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The RNA-binding protein RbpB is a central regulator of polysaccharide utilization in gut BacteroidesbioRxiv - Microbiology 2023Quote: ... 20 pmol of the dephosporylated RNA were 5’-end-labeled (20 µCi of 32P-γATP) in a 20 µL reaction for 1 h at 37°C using 1 U polynucleotide kinase (NEB). Labeled RNA was purified on a G50-column (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: Radiolabeling of oligonucleotides was carried out for 1 h at 37 °C using T4 polynucleotide kinase (NEB), 0.25 μM oligonucleotide ...
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A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... top and bottom oligonucleotides for a single R-S-R or dead end oligonucleotide pairs were combined 1 µL:1 µL and were phosphorylated in a 50 µL reaction using T4 Polynucleotide Kinase (NEB M0201L) for 2 h at 37 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA fragments were treated with T4 polynucleotide kinase (NEB), ligated to the linker (NEB Universal miRNA Cloning Linker ...
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bioRxiv - Microbiology 2019Quote: ... amplified fragment was phosphorylated using T4 polynucleotide kinase (NEB) and then ligated using T4 DNA ligase (NEB) ...
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bioRxiv - Molecular Biology 2021Quote: ... and dephosphorylated using T4 Polynucleotide Kinase (M0201, NEB, USA) prior to isopropanol precipitation and resuspension in 10 mM Tris [pH8]) ...
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bioRxiv - Molecular Biology 2021Quote: ... Total RNA was treated with T4 Polynucleotide Kinase (NEB) and rRNA depleted using an in-house protocol and cDNA library preparation was preceded by ultrasonic fragmentation ...
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bioRxiv - Molecular Biology 2021Quote: ... The oligos were radiolabeled by T4 polynucleotide kinase (NEB) with adenosine [γ32P]-triphosphate (6000 Ci/mmol ...
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bioRxiv - Genomics 2020Quote: ... 5′ end phosphorylation using T4 polynucleotide kinase (NEB, M0201L), (4 ...
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bioRxiv - Genomics 2020Quote: ... RNA overhangs are repaired with T4 Polynucleoide Kinase (NEB) with no ATP at 37°C for 20 min ...
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bioRxiv - Synthetic Biology 2020Quote: ... gDNAs were either phosphorylated using T4 Polynucleotide Kinase (NEB) in lab or phosphorylated guides were purchased from IDT ...
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bioRxiv - Bioengineering 2019Quote: ... 0.1 U/µL T4 Polynucleotide Kinase (T4 PNK, NEB), 1x CutSmart® buffer (NEB).
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bioRxiv - Plant Biology 2021Quote: ... oligos were end-labelled using T4 polynucleotide kinase (NEB) with γ-32P ...
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bioRxiv - Genetics 2020Quote: ... The oligonucleotides were phosphorylated with T4 polynucleotide kinase (NEB), annealed and inserted in the linearized vector with T4 DNA ligase (NEB) ...
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bioRxiv - Microbiology 2020Quote: ... and 4 μL T4 polynucleotide kinase (New England BioLabs) in a 100 μL reaction for 2 hours and purified using P-30 spin columns (BioRad) ...
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bioRxiv - Developmental Biology 2022Quote: ... and 5’ end-labeled using T4 polynucleotide kinase (NEB) and [γ-32P] ATP ...
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bioRxiv - Biochemistry 2022Quote: ... followed by KLD (Kinase, Ligase, and DpnI) digestion (NEB). Detailed primers used for TatA variant construction can be found in Supplemental Table 2 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and phosphorylated using T4 polynucleotide Kinase (New England Biolabs). The phosphorylated DNA was cleaned and concentrated again as above and then ligated using T4 DNA Ligase (New England Biolabs) ...
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bioRxiv - Biophysics 2019Quote: ... and T4 polynucleotide kinase were also purchased from NEB. All other chemicals were purchased from Sigma-Aldrich (St ...
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bioRxiv - Molecular Biology 2019Quote: ... [γ32P]ATP and T4 polynucleotide kinase (New England Biolabs).