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Citations for New England Biolabs :
51 - 100 of 2032 citations for Pops Par Solution Unlabeled 2000 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2022Quote: ... the pre-cleared lysates were collected and mixed with the biotin labeled pre-miRNA or biotin labeled shuffled pre-miRNA as negative control in the final concentration of 8.23 ng/ml and ATP (New England Biolabs) in the final concentration of 0.5 mM ...
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bioRxiv - Evolutionary Biology 2021Quote: ... PCR reactions contained 10 ng of template DNA and 1X 5PRIME HotMasterMix (Quanta Biosciences) as well as 0.4 mg ml-1 BSA (NEB) and 0.4 μM of each primer ...
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bioRxiv - Developmental Biology 2023Quote: ... The PCR product was injected into N2 worms at 10-20 ng/μl with 50 ng/μl pRF4 and 50 ng/μl DNA ladder (NEB, Ipswich, MA). The pRF4 plasmid produces a dominant roller phenotype ...
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bioRxiv - Genetics 2020Quote: ... 10 mL of a 10% BSA solution was incubated with 2.5 μL of Proteinase K (NEB P8107S) for 30 minutes at room temperature (∼22°C) ...
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bioRxiv - Molecular Biology 2020Quote: ... mouse monoclonal anti-MBP (1:2000, NEB), rabbit polyclonal anti-Spo11 (1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: ... and 2000 U T4 DNA Ligase (NEB) in 1x T4 DNA Ligase buffer (NEB) ...
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bioRxiv - Cancer Biology 2022Quote: PARP1 (500 ng, Tulip Biolabs), sheared Salmon Sperm DNA (100 ng ...
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bioRxiv - Biochemistry 2022Quote: ... 5 ng ET SSB (NEB), 4 nM phi29 DNAP in a 100 μl reaction and incubated for 3 h at 30°C ...
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bioRxiv - Genomics 2024Quote: ... Genomic DNA was harvested by discarding culture media and adding lysis buffer consisting of 20 mM Tris pH 8 and 0.1% Triton X-100 (MilliporeSigma T9284) with 60 ng/mL of Proteinase K (New England Biolabs P8107S) added immediately prior to use ...
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bioRxiv - Molecular Biology 2021Quote: ... their nuclei were isolated via centrifugation then resuspended in a 0.5 ml solution comprising 1.2 × restriction enzyme NEBuffer™ r3.1 (NEB) containing 0.3% SDS ...
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bioRxiv - Microbiology 2021Quote: ... 15 μL of PCR product were mixed with 5 μL of a 5X Exo-SAP solution (15% Shrimp Alkaline Phosphatase – 1000U/ ml – NEB, 10% Exonuclease I – 20000 U/ ml – NEB, 45% glycerol 80% and 30% dH2O). and incubated for 30 min at 37°C and for 15 min at 80°C ...
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bioRxiv - Genetics 2024Quote: ... A mixture of 20 ng/μL transgene and 70 ng/μL 1 kb ladder (NEB) filler DNA was microinjected into CZ26711 young adult hermaphrodites ...
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bioRxiv - Genetics 2022Quote: ... 2004) PCR reactions were performed in MiniAmp Plus Thermal Cycler with Q5® High-Fidelity DNA polymerase (2000 U/mL) (NEB Ipswich, Massachusetts) according to the manufacturer’s instructions ...
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A small molecule reveals role of insulin receptor-insulin like growth factor-1 receptor heterodimersbioRxiv - Cell Biology 2021Quote: ... Reverse transcription of RNA to cDNA was performed by combining 500 ng/ mL of the RNA samples with the LunaScript™ RT SuperMix (5X) (NEB) in 20 μL aliquots ...
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bioRxiv - Synthetic Biology 2023Quote: ... the PURExpress® kit containing Solution A and Solution B (NEB) was used to prepare the transcription-translation reaction ...
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bioRxiv - Cell Biology 2022Quote: ... and micrococcal nuclease (stock 2000 U/μL, NEB). 6* protein loading sample buffer without DTT or SDS was added to samples before loading into a precast native 4-12% tris glycine gel (Invitrogen ...
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bioRxiv - Genomics 2022Quote: ... and 2000 U T4 DNA ligase (NEB M0202M) was added ...
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bioRxiv - Microbiology 2023Quote: ... and Micrococcal nuclease (2000 U, New England Biolabs) to digest remaining free-floating eukaryotic and bacterial nucleic acids ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and resuspended in 50 µL of 1 mg/mL RNAse A solution (New England Biolabs, Ipswich, Massachusetts, United States) and statically incubated at 37 °C for 3 h ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were treated with 25 µg Proteinase K (1.25 µL of 20 mg/mL stock solution, New England Biolabs) and incubated at 50 °C for 20 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... 7.5 ng and 15 ng of digested DNA were amplified with 7.5 U φ29 polymerase (NEB) in 1X φ29 buffer (NEB ...
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bioRxiv - Cell Biology 2020Quote: ... Cas9 (416 ng/μl, NEB, USA). F1 worms carrying roller phenotype were preselected and cloned 4-6 days after the injection ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.1 ng of Lambda DNA (NEB) digested with BstEII (NEB) ...
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bioRxiv - Biophysics 2023Quote: ... Trypsin (0.15 ng) (New England Biolabs) was added following the removal of an aliquot at the zero-time point ...
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bioRxiv - Genomics 2023Quote: ... 1000 ng of Lambda Phage (NEB) was added to the tube ...
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bioRxiv - Synthetic Biology 2019Quote: ... DNA oligonucleotides were diluted (and mixed) to the desired concentration in buffer solution with 2 mg/mL BSA (Molecular Biology Grade, New England Biolabs) to minimize DNA adsorption to tubing and pipette tips and loaded to inlet port 3 ...
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bioRxiv - Microbiology 2021Quote: ... 15 μL of PCR product were mixed with 5 μL of a 5X Exo-SAP solution (15% Shrimp Alkaline Phosphatase – 1000U/ ml – NEB, 10% Exonuclease I – 20000 U/ ml – NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... 10 mM sodium ascorbate before collecting them by scraping in 1 mL of the same solution with Murine RNase Inhibitor (1:1000, NEB). Cells were collected in a microcentrifuge tube ...
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bioRxiv - Cancer Biology 2023Quote: ... 1:100 10 mg/mL BSA in distilled water) with 0.75 µL (150 U) micrococcal nuclease enzyme solution (1:10 micrococcal nuclease (NEB, USA) in micrococcal nuclease reaction buffer) ...
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bioRxiv - Neuroscience 2024Quote: ... the tissue was incubated in 5 mL of a clearing solution comprising Clearing Premix (Vizgen, PN 20300003) and 50 μL Proteinase K (New England BioLabs) at 37°C in a humidified benchtop incubator until the tissue was cleared.
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bioRxiv - Cell Biology 2020Quote: ... 2000 ligation units of T4 DNA ligase (NEB, M0202), and 30 units of T7 DNA polymerase (NEB ...
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bioRxiv - Microbiology 2019Quote: ... and 30 ng/ul Proteinase K (NEB)) ...
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bioRxiv - Bioengineering 2023Quote: ... and 60 ng/µL Proteinase K (NEB)) ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.5 ng for CTCF and 10 ng for input) were processed using NEBNext ChIP-seq library (New England Biolabs) with manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... we combined 50 ng of backbone with 25 ng of annealed oligo along with T4 ligase buffer (NEB #B0202S), T4 ligase (#M0202S) ...
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bioRxiv - Bioengineering 2023Quote: ... we combined 50 ng of backbone with ∼25 ng of annealed oligo along with T4 ligase buffer (NEB #B0202S), T4 ligase (#M0202S) ...
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bioRxiv - Developmental Biology 2023Quote: ... along with 2.5 ng/μl pCFJ90(Pmyo-2::mCherry) and 77.5 ng/μl of 2-Log DNA ladder (New England BioLabs), to generate two independent strains expressing Ex[Pcol-10::mCherry::his-58::SL2::drl-1 cDNA] (DLS515 ...
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Targeted Perturb-seq Reveals EGR1 and FOS as Key Regulators of the Transcriptional RAF-MAPK ResponsebioRxiv - Systems Biology 2024Quote: ... 100 ng digested pMB1-10x and 2.4 ng amplified sgRNA library insert were assembled using Gibson Assembly Master Mix (NEB) in a 20 μL reaction for 30 min ...
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bioRxiv - Synthetic Biology 2023Quote: ... The ligation reaction was performed with 100 ng of digested vector backbone and 70.8 ng of inserts using T4 DNA ligase (NEB) at 16°C for 15 hours.
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bioRxiv - Cell Biology 2023Quote: ... and one in-solution (NEB) DNase digestions were performed ...
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bioRxiv - Synthetic Biology 2024Quote: ... 3.2 μL solution A (NEB), 1 μL factor mix (NEB) ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were split in half for MNase (2000 units, NEB) treatment at 27°C for 30 min or mock treatment on ice ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1 μL Micrococcal Nuclease (2000 U) (New England Biolabs). The solution was incubated at 37°C for at least 3-4 hrs ...
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bioRxiv - Genomics 2020Quote: ... we heated samples to 95°C for 3min and snap-cooled on an ice slush before gently adding 0.5μl of enzyme solution (8,000 U/ml Bst DNA Polymerase Large Fragment, New England Biolabs, USA, in 1X amplification buffer) into the aqueous droplet.
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bioRxiv - Biochemistry 2021Quote: ... to 10 μl of the plasma/stop solution 70 μl of mixture consisting of 75 mU/ml ATP sulfurylase (New England Biolabs, Cambridge, MA), 1 μmol/L APS (Santa Cruz Biotechnology ...
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bioRxiv - Molecular Biology 2023Quote: ... was immersed in a 10-fold volume of digestion solution with 8 U/mL Proteinase K (P8107S, New England Biolabs, Ipswich, USA) in digestion buffer containing 50 mM Tris pH 8.0 ...
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bioRxiv - Cell Biology 2021Quote: ... 50 ng of vector and 150 ng of each fragment were mixed with 3 μl of HiFi DNA Master Mix (NEB) and incubated at 50°C for 1 hour to form the new pCDH-TagBFP-T2A-myc-BirA*-Tensin3 construct ...
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bioRxiv - Cancer Biology 2021Quote: ... We assembled a 20 ng amplified cassette into a 50 ng digested vector in a 20 μL Gibson reaction (NEB). The individual construct was verified using Sanger sequencing (Genewiz).
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bioRxiv - Microbiology 2020Quote: ... The resulting gDNA was used as template for PCR amplification of the shRNA encoding regions using the Fw-NGS and the Rev-NGS primers (IDT Technologies) and the Phusion High Fidelity DNA polymerase (NEB). Three PCRs per condition were performed and the amplicons combined ...
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bioRxiv - Genomics 2019Quote: ... D7 and D5 pre-annealed indexed adapters (2 ng and 200 ng, respectively) and T4 DNA ligase (400 U, NEB), and were incubated at 16°C overnight ...