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Citations for New England Biolabs :
251 - 300 of 2032 citations for Pops Par Solution Unlabeled 2000 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... 100 ng of genomic DNA was treated with McrBC enzyme (New England Biolabs, M0272L) at 37°C overnight and then for 10 min at 75°C to deactivate the enzyme ...
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bioRxiv - Biochemistry 2020Quote: ... 10-50 ng of mononucleosomal DNA was incubated with 1.25 Units Taq polymerase (NEB), 3.75 Units T4 DNA polymerase (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... 500 ng of genomic DNA was digested with MspI+EcoRI and HpaII+EcoRI (NEB) in parallel reactions ...
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bioRxiv - Developmental Biology 2022Quote: ... 50 ng PCR product was incubated with 2 µL 10X NEBuffer 2 (NEB, B7002S) and nuclease-free water adding up to 19 µL using the following program ...
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bioRxiv - Genomics 2022Quote: 500 ng to 1 μg gDNA were cleaved by SacI-HF (New England Biolabs) for 15 min at 37 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... 900 ng RNA was depleted of rRNA using the NEBNext rRNA Depletion kit (NEB). RNA-seq libraries were prepared from an equal amount of ribo-depleted RNA using the NEBNext Ultra II Directional RNA Library Prep kit ...
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bioRxiv - Biochemistry 2020Quote: ... 10-50 ng of mononucleosomal DNA was incubated with 1.25 Units Taq polymerase (NEB), 3.75 Units T4 DNA polymerase (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... and NGS libraries were quantified by qPCR using the NEBNext Library Quant Kit (NEB). Illumina sequencing was performed with a NextSeq mid-output kit with 150-cycle single-end reads and automated adaptor trimming and demultiplexing (Illumina).
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bioRxiv - Cancer Biology 2020Quote: ... 400 ng of RNA was digested by 5 U of RppH (New England Biolabs) for 2 h at 37 °C ...
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bioRxiv - Genomics 2020Quote: ... ddPCR absolute quantification was performed using 15 ng of DraI or AluI (NEB, US) pre-restriction digested DNA in a 20ul reaction mix containing 1x ddPCR Supermix for Probes (BioRad ...
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bioRxiv - Molecular Biology 2020Quote: ... 100 ng of isolated DNA was digested with MspI restriction enzyme (NEB, Ipswich, MA), carried out end-repair/adenylation (NEB ...
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bioRxiv - Microbiology 2022Quote: ... The final injection mix contained 300 ng/μL spCas9 protein (M0386, New England Biolabs), 40 ng/μL of each sgRNA (3 targeting exon 5 and 7 of Dicer2 and 4 targeting the kmo gene ...
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bioRxiv - Neuroscience 2023Quote: ... ∼100 ng of RNA was treated with NEBNext rRNA Depletion Kit (New England Biolabs). RNA-Seq was performed using libraries prepared with NEBNext Ultra™ Directional RNA Library Prep Kit for Illumina (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... Illumina NGS libraries were prepared using the NEBNext Ultra II DNA Kit (NEB, E7645S), and sequenced for 50-nt SE reads ...
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bioRxiv - Developmental Biology 2023Quote: ... Injection mixtures containing 500 ng/µl of Cas 9 protein (NEB; Cat. no.: M0641) and 300 ng/µl of guide RNA were prepared and injected into eggs within 4 hours of egg laying ...
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bioRxiv - Molecular Biology 2023Quote: ... NGS libraries were pooled and quantified by qPCR using NEBNext Library Quant Kit (NEB). Sequencing was performed with NextSeq high-output kit with 75/150-cycle single-end reads and automated adaptor trimming and demultiplexing (Illumina ...
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bioRxiv - Genomics 2023Quote: ... 500 ng of genomic DNA was digested with MspI+EcoRI and HpaII+EcoRI (NEB) in parallel reactions ...
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bioRxiv - Genetics 2023Quote: ... and NGS libraries were quantified by qPCR using the NEBNext Library Quant Kit (NEB). Illumina sequencing was performed using the NextSeq platform with automated demultiplexing and adaptor trimming (Illumina).
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bioRxiv - Microbiology 2023Quote: ... RNA (200 ng) was reverse transcribed into cDNA using LunaScript®RT SuperMix (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: 500 ng of sgRNA was combined with 20 µM EnGen Cas9 NLS (NEB #M0646) in 1x Cas9 Nuclease Reaction Buffer (NEB #B0386A ...
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bioRxiv - Biophysics 2023Quote: ... 100 ng or less template DNA and 24 μL Taq DNA polymerase (NEB M0273L). Purify and concentrate the PCR DNA product by ethanol precipitation ...
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bioRxiv - Developmental Biology 2024Quote: ... ∼300 ngs of genomic DNA were digested with 100U of MspI (New England Biolabs), and cleaned up using QIAquick PCR purification columns (Qiagen) ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA (>300 ng) was reverse transcribed using the Lunascript RT Supermix Kit (NEB, E3010).
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bioRxiv - Biophysics 2023Quote: 12mer arrays (40 ng) were digested with 1 U of MNase (New England Biolabs) in a 10 μL reaction for varying digestion times (2.5 to 10 minutes ...
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bioRxiv - Microbiology 2020Quote: ... and then added up to 5 fmol DNA (2000:1000:1 RNA:protein:DNA ratio) and NEBuffer 3.1 (NEB) to 1X ...
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bioRxiv - Cell Biology 2020Quote: ... then deglycosylated with 2000 U of Peptide-N-Glycosidase F (PNGase F) (New England BioLabs, MA, USA). All samples were incubated for 16 hours at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 nl of a solution containing 10µM EnGen Cas9 NLS (NEB) and 100 ng/µl of gRNAs was injected at the one-cell stage ...
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bioRxiv - Neuroscience 2022Quote: ... These solutions were added to 100 units of TEV protease (NEB) in aggregation buffer ...
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bioRxiv - Microbiology 2021Quote: ... and amplified with OneTaq 2x MasterMix solution (New England Biolabs, MA) mixed with T ...
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bioRxiv - Bioengineering 2023Quote: ... 200 uM of deoxynucleotide (dNTP) solution mix (New England Biolabs (NEB), cat# N0447L) ...
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bioRxiv - Bioengineering 2023Quote: ... 200 uM of deoxynucleotide (dNTP) solution mix (New England Biolabs (NEB), cat# N0447L) ...
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bioRxiv - Cell Biology 2023Quote: A 40 µL solution of 25% (v/v) amylose resin (NEB) was co-incubated with 500 nM MBP-RH domain and 4 µM Alexa Fluor 647-labeled RAB7A (life technologies ...
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FACS-Sortable Picoreactors for Ultra High-throughput Screening of Catalysts in Biphasic EnvironmentsbioRxiv - Bioengineering 2024Quote: ... the two inner solutions were comprised of 1) PURExpress reagents (NEB) or 2 ...
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bioRxiv - Synthetic Biology 2024Quote: ... and dNTPs solution mix (Cat. # N0447) from New England Biolabs (NEB). Nuclease free water (Merck ...
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bioRxiv - Biochemistry 2021Quote: ... Nb35–His (10µg/ml) and apyrase (25mU/ml, NEB); the suspension was incubated for 1h at room temperature ...
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bioRxiv - Cancer Biology 2019Quote: ... ligation was carried out at RT for 2h using 2000 U of T4 DNA ligase (New England Biolabs). DH5α cells (Thermo Fisher ...
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Essential roles of the ANKRD31-REC114 interaction in meiotic recombination and mouse spermatogenesisbioRxiv - Genetics 2023Quote: ... P5 adapters were ligated to the ends with 1 μl of 2000 U/μl T4 DNA Ligase (NEB) at 16 °C for 20 hr ...
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bioRxiv - Genetics 2021Quote: ... 0.5 μM MPRA_v3_F and MPRA_v3_20I_R primers (Supplementary Table 14) and 2 ng BSA (NEB, B9000). PCR master mix was emulsified by vortexing with 220 μL Tegosoft DEC (Evonik) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 100 ng of DNA were digested using PstI and MspI (New England Biolabs, Ipswich, MA) after which barcoded adapters amenable to Illumina sequencing were added by ligation with T4 ligase (New England Biolabs ...
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bioRxiv - Molecular Biology 2020Quote: ... or 500 ng of chromatin-bound or nucleoplasmic RNA with 2 µl Quick CIP (NEB) in a total volume of 20 µl for 90 min at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 20 ng of the resulting DNA was incubated with 7.5 U φ29 DNA polymerase (NEB) in reaction buffer [1 µg/µl BSA ...
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bioRxiv - Biochemistry 2020Quote: 100 ng of purified tsRNAs were treated with 0.5 U/µL T4 polynucleotide kinase (NEB) and 166 µM ATP (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... we performed RFLP on 200 ng of MSP PCR-product using Hph1 (New England Biolabs) restriction enzyme accordingly to manufacture’s instruction ...
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bioRxiv - Biochemistry 2021Quote: ... 1 ng/μl of template DNA and 1 U of Phusion polymerase (New England Biolabs). The mutant PCR fragments were then cloned into pSpurMS2 (a gift from Dr ...
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bioRxiv - Plant Biology 2020Quote: ... 100 ng of DNA was digested using PstI and BfaI (New England Biolabs, Ipswich, MA) after which barcoded adapters amenable to Illumina sequencing were added by ligation with T4 ligase (New England Biolabs ...
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bioRxiv - Molecular Biology 2021Quote: ... and 750 ng of each sample was digested with 40 U of EcoRI (NEB, R0101L) and 2 μl of 10× EcoRI buffer in a 20 μl reaction for 12 hours ...
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bioRxiv - Plant Biology 2021Quote: ... 300 ng of gRNA transcript was bound to a purified Cas9 protein (New England Biolabs) for 10 minutes at 25° C ...
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bioRxiv - Genetics 2021Quote: ... 250 ng – 1 μg of RNA samples were digested with Nucleoside Digestion mix (NEB, M069S) for 2 hr at 37°C ...
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bioRxiv - Microbiology 2019Quote: ... 50 ng of processed DNA was mixed with NEBuffer 3.1® (New England Biolabs®), pH 7.9 ...
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bioRxiv - Microbiology 2019Quote: ... 500 ng of isolated DNA was treated with CutSmart buffer® (New England Biolabs®), pH 7.9 ...