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Citations for New England Biolabs :
401 - 450 of 2032 citations for Pops Par Solution Unlabeled 2000 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... A 1 ml aliquot of cells was incubated with 1 μl M13KO7 (1011 plaque-forming units per ml, pfu/ml) (NEB) for 10 min on ice to allow attachment ...
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bioRxiv - Developmental Biology 2024Quote: ... 47 ml water) and 4 µL 10mg/ml Proteinase K (P8108S, NEB). The samples were incubated for 8 hours at 55 °C followed by 10 min at 98 °C to inactivate the Proteinase K ...
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bioRxiv - Immunology 2021Quote: ... followed by ligation to the pF30P linker (Liu et al., 2000) via a splint oligonucleotide by T4 DNA ligase (NEB). After lambda exonuclease digestion to remove splint and unincorporated linker ...
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bioRxiv - Cell Biology 2022Quote: SDS-PAGE and Western blot analysis were performed as previously described (Alpy et al, 2005) using the following antibodies: rabbit anti-GFP (1:2000; TP401, Torrey Pine Biolabs), rabbit anti-MOSPD2 (1:250 ...
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bioRxiv - Developmental Biology 2020Quote: DNA was extracted from embryonic membranes or tail tissue using the HotShot method for 15 minutes (Truett et al., 2000) and 2 µl DNA was used for PCR with Phusion polymerase (New England Biolabs). For Geminin and Cre PCRs ...
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bioRxiv - Evolutionary Biology 2020Quote: ... with tailed primers and cloned the amplicon into pBac{3XP3-ECFPafm} (Horn and Wimmer 2000) digested with AscI and Bsu36I restriction enzymes using Gibson Assembly Master Mix (NEB). Primers are included in Supplemental Table 1 ...
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bioRxiv - Genetics 2020Quote: ... We used the restrict function in EMBOSS (version 6.6.0) (Rice, Longden, and Bleasby 2000) in conjunction with the REBASE database published by New England BioLabs (NEB; version 808) (Roberts and Macelis 1999 ...
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bioRxiv - Bioengineering 2022Quote: ... pRED-MAD7 was constructed by integration of this fragment into NcoI-linearized pKD46 (Datsenko and Wanner, 2000) by Gibson assembly (New England Biolabs).
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bioRxiv - Molecular Biology 2023Quote: ... in 100 µL MNase reaction mix (87 µL ddH2O, 10 µL 10x MNase buffer, NEB, 1 µL 100x BSA, 2 µL 2000 U/µL MNase, NEB). Digests were centrifuged (5 min ...
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bioRxiv - Molecular Biology 2024Quote: ... and sequenced on a NextSeq 2000 instrument (Srp2-HTP & Dbp2-HTP) or with the NEBNext Fast DNA Library Prep Set for Ion Torrent Kit (NEB) and sequenced on an Ion Torrent Proton (Life Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... 4 ml ScaI (BioLabs), followed by 3 h of incubation with a fresh portion ...
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bioRxiv - Molecular Biology 2020Quote: ... BSA (2mg/ml, NEB), dextran sulfate (10% ...
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bioRxiv - Biochemistry 2022Quote: ... 0.1mg/mL BSA (NEB), 0.1 mg/mL Baker’s Yeast tRNA (Sigma R5636) ...
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bioRxiv - Microbiology 2023Quote: ... 20μl/ml DNase (NEB) and RNase cocktail (Amersham) ...
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bioRxiv - Bioengineering 2024Quote: ... – 400 U/mL (NEB); Jack bean hexosaminidase (JBH ...
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bioRxiv - Evolutionary Biology 2020Quote: Purified DNA library (250 ng) was cut with the Hind III and Bam HI restriction enzymes (NEB) for 1h at 37 °C in a reaction mixture containing 1X of the NEB cut smart buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 ng of mRNA was decapped using either tobacco acid pyrophosphate (250 U; Epicenter) or RppH (NEB) in buffer provided by the supplier and then dephosphorylated by Antarctic phosphatase (NEB) ...
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bioRxiv - Molecular Biology 2020Quote: ... 20 ng of DNA was digested with 6 to 12 U of the restriction enzyme PvuII (NEB) overnight at 37°C.
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bioRxiv - Microbiology 2019Quote: ... A total amount of 25 ng cDNA was used in a standard PCR using Taq-Polymerase (NEB; number of cycles for product amplification ...
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bioRxiv - Genomics 2021Quote: ... 50 ng of DNA was digested using the 5-bp cutter ApeKI (New England Biolabs, Ipswich, MA) after which barcoded adapters were added by ligation with T4 ligase (New England Biolabs ...
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bioRxiv - Genomics 2019Quote: 200 ng purified genomic DNA was glucosylated by incubating with 20U of BGT (NEB, Ipswich MA, USA) for 2 h at 37°C (for 5-hmC detection) ...
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bioRxiv - Genetics 2021Quote: ... Total RNA (250 ng) was depleted for ribosomal RNA using NEBNext® rRNA DepletionKit (New England BioLabs) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... Approximately 200 ng/μl of RNA was used for cDNA synthesis by M-MuLV reverse transcriptase (NEB) and 3′ oligo-dT primer ...
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bioRxiv - Microbiology 2021Quote: ... and 20 ng of mRFP PCR products were incubated with Gibson master mix (New England Biolabs, #E2611S) at 50°C for 1 hour ...
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bioRxiv - Cell Biology 2021Quote: ... 500 ng of digested DNA were incubated with 7.5 U of phi29 DNA polymerase (New England Biolabs) in presence of dATP ...
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bioRxiv - Cancer Biology 2020Quote: ... 100 ng of total RNA was used to prepare cDNA using ProtoScript II Reverse Transcriptase (NEB #M0368) with oligo(dT ...
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bioRxiv - Biochemistry 2020Quote: ... 100 ng of gel purified DNA was used as a template for in vitro transcription (NEB E2040S) carried out according to the manufacturer’s instructions with the addition of 0.1µl of Cy3 (Sigma PA53026 ...
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bioRxiv - Plant Biology 2020Quote: A 200-ng quantity of genomic DNA was digested by the methylation-sensitive enzyme HaeIII (NEB, R0108S) in a 20-μl reaction mixture at 37℃ for 20 h ...
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bioRxiv - Bioengineering 2021Quote: ... 50 ng input genomic DNA was PCR amplified with Q5 High-Fidelity DNA Polymerase (New England Biolabs): (98 °C ...
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bioRxiv - Genomics 2021Quote: ... 10 ng A-tailed gel-isolated PCR product and 1 µl T4 DNA ligase (New England Biolabs) were mixed in a total reaction volume of 10 µl and incubated for 15 min at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... 400 ng of eluted DNA fragments were end-labeled with T4 Polynucleotide Kinase (T4 PNK) (NEB M0201L) and 10 µCi of [α-32P]-ATP (Perkins Elmer NEG002A ...
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bioRxiv - Immunology 2020Quote: ... mRNA was isolated from purified 1 ng total RNA using oligo-dT beads (New England Biolabs, Inc). NEBNext Ultra™ RNA kit was used for full-length cDNA synthesis and library preparation ...
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bioRxiv - Molecular Biology 2021Quote: ... Ribosomal RNA was depleted from 300 ng total RNA using the NEBNext rRNA Depletion Kit (NEB, #E6310L), and libraries were generated for next-generation sequencing using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Genomics 2021Quote: ... mRNA was isolated from 100 ng of total RNA per sample using oligo dT magnetic beads (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... 5 ng of α-HA ChIP or input DNA were end-repaired with T4 DNA polymerase (NEB), Klenow DNA polymerase (NEB) ...
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bioRxiv - Genomics 2023Quote: ... An NGS library was generated using an NEB Ultra II DNA Library Prep kit (NEB, Ipswich, MA) with an Illumina compatible y-adaptor ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 ng DNA was used to prepare libraries using the NEBNext Ultra II DNA kit (NEB E7645S) as described with modifications ...
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bioRxiv - Genetics 2023Quote: ... 250 ng of genomic was used in each PCR1 amplification with Q5 High-Fidelity DNA Polymerase (NEB) for 15 cycles ...
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bioRxiv - Genetics 2023Quote: Next-generation sequencing (NGS) amplicons were prepared by PCR amplification using Q5 High-Fidelity DNA Polymerase (NEB). 250 ng of template DNA was amplified in 15 cycles during the PCR1 step ...
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bioRxiv - Neuroscience 2023Quote: 150 ng of total RNA was applied to the NEBNext rRNA Depletion Kit (New England Biolabs, #E6310L) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 µl genomic DNA (input 90 ng) from naïve hESCs were digested with LpnPI (New England Biolabs) generating 32 bp fragments around the fully methylated recognition site containing a CpG ...
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bioRxiv - Cancer Biology 2023Quote: ... NGS libraries were prepared using NEBnext Ultra II DNA library preparation kit for Illumina (New England Biolabs) and quantified with using a TapeStation (Agilent Genomics) ...
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bioRxiv - Molecular Biology 2024Quote: ... 20-50 ng of purified RNA was processed by NEBNext rRNA Depletion Kit (New England BioLabs, Inc.) for removing ribosomal RNA ...
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bioRxiv - Biochemistry 2023Quote: ... PCR reactions were purified using GeneAid and ∼100 ng of DNA subjected to digestion by NaeI (NEB). Clones that showed no apparent digestion product (due to the modification of the NaeI restriction site ...
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bioRxiv - Genomics 2023Quote: ... An NGS library was generated using an NEB Ultra II DNA Library Prep kit (NEB, Ipswich, MA) with an Illumina compatible y-adaptor ...
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bioRxiv - Immunology 2023Quote: ... The library was diluted to 50 ng/uL in water and electroporated to competent (NEB DH5a cells) cells with an efficiency of ≥109 cfu/ug ...
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bioRxiv - Molecular Biology 2023Quote: ... 150 ng of deaminated DNAs were digested to nucleosides with the Nucleoside Digestion Mix (NEB, Ipswich, MA) following manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2021Quote: ... 1% NP-40, 1 mM DTT, 0.1 mg/ml cycloheximide, 1.25 mg/ml heparin, 400 U/ml NEB murine RNase inhibitor) and dounced ...
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bioRxiv - Molecular Biology 2020Quote: ... The complexation reaction was carried out in a solution containing 1X NEBuffer 2.1 (NEB) (composed of 100 mM NaCl ...
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bioRxiv - Molecular Biology 2021Quote: ... This was repeated with a 1:200 pA-Dam solution (∼60 NEB Dam units), followed by 2 wash steps ...